公开(公告)号：WO1991019729A1

公开(公告)日：1991-12-26

申请号：PCT/US1991003976

申请日：1991-06-05

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  ADAMS, Craig, W.

IPC: C07H21/00

CPC classification number: C07H21/00 ,  C12Q1/6816 ,  G01N33/532 ,  G01N33/58 ,  C12Q2563/131 ,  C12Q2563/125

Abstract: Nucleic acid probes and protein probes are disclosed. The nucleic acid probe comprises a probe polynucleotide, a charged hapten label, and a binding moiety. The protein probe comprises a probe protein, a charged hapten label and a binding moiety. The charged hpaten label joined to the binding moiety can comprise a negatively charged sulfophenylhydrazine tag compound. Polyclonal antibodies and monoclonal antibodies with specific affinity for the charged hapten labels are disclosed as are hybridomas capable of making the monoclonal antibodies. Methods and kits are disclosed for making the nucleic acid probes, making the protein probes, detecting a capture polynucleotide of the nucleic acid probe and detecting a capture molecule of the protein probe.

Abstract translation: 公开了核酸探针和蛋白质探针。 核酸探针包含探针多核苷酸，带电半抗体标记和结合部分。 蛋白质探针包括探针蛋白，带电半抗体标记和结合部分。 连接到结合部分的带电荷的标记物标签可以包含带负电荷的磺基苯肼标记化合物。 公开了对加入的半抗原标记具有特异性亲和力的多克隆抗体和单克隆抗体也是能够制备单克隆抗体的杂交瘤。 公开了用于制备核酸探针，制备蛋白质探针，检测核酸探针的捕获多核苷酸并检测蛋白质探针的捕获分子的方法和试剂盒。

公开(公告)号：WO1990013819A1

公开(公告)日：1990-11-15

申请号：PCT/US1990001964

申请日：1990-04-10

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  BAKER, Josefina, T. ,  SHOCKEY, David, R. ,  DIBIASE, Anthony

IPC: G01N33/72

CPC classification number: B01L3/5055 ,  G01N33/726 ,  Y10S435/805

Abstract: A method and specimen slide for obtaining fecal occult blood specimens. The slide includes first and second portions each including front and back panels. The first portion includes one or more apertures formed through its front panel which exposes a reagent-carrying sheet and the back panel of the first portion includes a flap that may be opened for the application of additional reagents to the back of the sheet. The second portion includes an aperture in its front panel and a flap formed in its back panel. A sheet is carried between the front and back panels of the second portion onto which a fecal specimen may be smeared. The sheet includes pre-perforated removable portions that may be easily accessed through the flap. As an alternative embodiment, the back panel of the second portion may include a removable tab to which is fixed the specimen sheet. The tab and specimen sheet may be removed for easy access to the removable portions of the specimen sheet.

公开(公告)号：WO1990000252A1

公开(公告)日：1990-01-11

申请号：PCT/US1989002372

申请日：1989-05-31

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  STERNBERG, James, C.

IPC: G01N33/543

CPC classification number: G01N33/54306 ,  G01N33/581 ,  G01N33/582

Abstract: Specific binding assays for measuring the concentration of an analyte in a sample involve forming a complex comprising a solid support, the analyte or an analogue of the analyte, and an enzyme. The enzyme is used to cleave non-isotopic labels that are directly detectable from a substrate/label conjugate to provide a plurality of released labels in the free state, which are then separated from conjugate containing unreleased label. The released labels are detected for measuring the concentration of the analyte in the sample. Preferably the analyte or analogue is connected to the solid support by a cleavable bond, so that the enzyme can be separated from the solid support for use in the enzymatic cleaving of labels from the conjugate. This preferred version reduces the adverse effect of non-specific binding on the sensitivity and accuracy of the assay.

Abstract translation: 用于测量样品中分析物浓度的特异性结合测定包括形成包含固体支持物，分析物或分析物的类似物的复合物和酶。 该酶用于切割可从底物/标记缀合物直接检测的非同位素标记，以提供游离状态的多个释放的标记，然后将其与含有未释放标签的缀合物分离。 检测释放的标签以测量样品中分析物的浓度。 优选地，分析物或类似物通过可切割键连接到固体支持物，使得酶可以与固体支持物分离，用于从缀合物酶标记的酶切割。 该优选的形式减少非特异性结合对测定的灵敏度和准确性的不利影响。

公开(公告)号：WO1989012826A1

公开(公告)日：1989-12-28

申请号：PCT/US1989002193

申请日：1989-05-19

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  OH, Chan, S. ,  STONE, Linda, J.

IPC: G01N33/82

CPC classification number: G01N33/82

Abstract: A competitive binding assay for vitamin B12 in a test sample gives a steep dose-response curve in the clinically significant concentration regions, yielding great accuracy. The assay uses immobilized vitamin B12 to compete with the vitamin B12 in the test sample for a binding partner. The quantity of the binding partner bound to the immobilized vitamin B12 can be measured and is inversely proportional to the concentration of free vitamin B12 in the test sample. The binding partner is typically biotinylated intrinsic factor prepared so that substantially all the molecules of intrinsic factor are biotinylated and the binding activity of the intrinsic factor for vitamin B12 is not diminished. The bound biotinylated intrinsic factor can then be determined by reacting it with avidin coupled to a reporter group. The reporter group is typically an enzyme such as horseradish peroxidase, but can be another detectable label. The immobilized vitamin B12 is typically bound to a solid support such as diazotized amine-derivatized beads, and can be bound through a protein linker. Other binding partners such as anti-vitamin B12 antibody are also usable. The binding partner can be labeled directly with the reporter group. The method is adaptable to the assay of serum, and methods of inactivating endogenous vitamin B12-binding proteins in serum are described.

Abstract translation: 测试样品中维生素B12的竞争性结合测定在临床显着的浓度区域提供了陡峭的剂量 - 反应曲线，产生了很高的准确性。 该测定使用固定的维生素B12与测试样品中的维生素B12竞争结合配偶体。 可以测量与固定化维生素B12结合的结合配偶体的数量，并与测试样品中游离维生素B12的浓度成反比。 结合配偶体通常是生物素化的固有因子，使得基本上所有内在因子的分子都被生物素化，并且维生素B12的内在因子的结合活性不会降低。 然后可以通过与结合报告基团的抗生物素蛋白反应来确定结合的生物素化内在因子。 记者组通常是诸如辣根过氧化物酶的酶，但可以是另一个可检测的标记。 固定的维生素B12通常与固体支持物结合，例如重氮化胺衍生的珠，并且可以通过蛋白质接头结合。 其他结合配偶体如抗维生素B12抗体也是可用的。 绑定对象可以直接与记者组进行标注。 该方法适用于血清测定，描述了血清中内源性维生素B12结合蛋白失活的方法。

公开(公告)号：WO1989004215A1

公开(公告)日：1989-05-18

申请号：PCT/US1988003720

申请日：1988-10-20

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  PENHASI, Harry, Aron

IPC: B04B05/00

CPC classification number: B04B5/0442 ,  B04B9/08 ,  B04B2005/0471 ,  F16D1/112

Abstract: The present invention provides an improved rotor for a centrifuge that operates by centrifugal elutriation. The rotor consists of a chamber assembly, a disk assembly, and a quick release mechanism. The chamber assembly is relatively light and it includes one or two separation chambers, tubing and a rotating seal. The disk assembly consists of the relatively heavy support structure. The two piece construction facilitates handling the parts of the rotor that generally need to the autoclaved. The chamber assembly has a novel structure that allows the rotor to easily be configured for operation with one or two chambers. The disk assembly has a unique strain relief that prevents structural cracking. The quick release mechanism is held in the latched position by centrifugal force during operation of the rotor.

公开(公告)号：WO1989003042A1

公开(公告)日：1989-04-06

申请号：PCT/US1988003372

申请日：1988-09-30

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  HARRIS, Paul, C. ,  OH, Chan, S.

IPC: G01N33/531

CPC classification number: G01N33/531 ,  G01N33/532 ,  G01N33/539 ,  G01N33/54313 ,  G01N33/94

Abstract: A bidentate conjugate has two different chemical moieties, or bidentate members, attached through an adequate spacer moiety. Each bidentate member acts as a small molecule ligand and is capable of specifically binding to a different macromolecular substance. The bidentate members are relatively small molecules, usually less than about 7,000 Daltons in size. The spacer moiety is selected so that both bidentate members can simultaneously bind to their respective specific binding partners. Where the specific binding partners are multivalent, large complexes can be formed. The formation of these complexes can be inhibited by the presence of an unconjugated monovalent bidentate member, such as free analyte from a test sample. The bidentate is of particular use in turbidimetric or nephelometric inhibition immunoassay procedures.

公开(公告)号：WO1989002591A1

公开(公告)日：1989-03-23

申请号：PCT/US1988003071

申请日：1988-08-31

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  McNEAL, Jack, Dennis ,  LEVINE, Mark ,  BEERY, J., Harvey

IPC: G01N27/28

CPC classification number: G01N27/403 ,  Y10T74/18288 ,  Y10T74/18312

Abstract: An analysis system including an ion selective electrode assembly, a sample container and a transport mechanism. The electrode assembly includes a reference electrode within a reference gel-filled well and ion selective electrodes disposed on an elongated portion of the electrode assembly. The sample container includes a body defining a plurality of reservoirs and a slot retaining an absorbant wiping and/or blotting material. The electrode assembly may be removably fixed relative to the transport mechanism. The transport mechanism includes a support table adapted to receive the sample container. The support table moves relative to the electrode assembly under the control of a cam to sequentially position the elongated portion of the electrode assembly within the reservoirs and the slot of the sample container.

公开(公告)号：WO1988010429A1

公开(公告)日：1988-12-29

申请号：PCT/US1988002066

申请日：1988-06-16

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  SEATON, Charles, Bret

IPC: G01N33/70

CPC classification number: G01N33/70 ,  Y10T436/147777 ,  Y10T436/25125 ,  Y10T436/2525

Abstract: A cold stable liquid reagent system suitable for use in a Jaffe method for the determination of creatinine comprises: a) a homogeneous liquid picric acid component comprising picric acid in an amount of no more than about 35 mM, such that the picric acid component remains visibly homogeneous at a temperature between about 2 to about 8°C for 30 days or more; b) a homogeneous liquid alkaline buffer component comprising: i) an alkaline material in sufficient amount to maintain the pH of the alkaline buffer component at more than about 13, for controlling the pH of the assay ready reagent formed by combining the picric acid and alkaline buffer components; ii) at least one pH buffer material; iii) a detergent for reducing interference by proteins in the Jaffe method; and iv) borate for reducing interference by carbohydrates in the Jaffe method; wherein the alkaline buffer components contains substantially no metallic cations such that the component remains visibly homogeneous at a temperature between about 2 to about 8°C for 30 days or more. Preferably the alkaline material is tetramethylammonium hydroxide, and the detergent is diethanolamine dodecyl sulfate.

公开(公告)号：WO1988007668A1

公开(公告)日：1988-10-06

申请号：PCT/US1988001046

申请日：1988-03-31

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  HOLMQUIST, Barton ,  SCOPES, Robert, K.

IPC: G01N01/28

CPC classification number: B01F13/0016 ,  B01F3/08 ,  G01N1/38

Abstract: This invention relates to a diluting and mixing device which is capable of diluting a first solution to produce a second solution which is mixed with an undiluted third solution, to reproducibly produce a unique series of combined solutions. Each solution in said series of combined solutions may vary only in the concentration of a single (selected) reactant, and typically, each successive solution becomes increasingly more concentrated in the selected reactant. By employing a modification in procedure, each successive solution in said series may become decreasingly less concentrated in the selected reactant. This invention further relates to an automated system comprising the device connected to a stepping motor so as to rapidly and reproducibly produce said series of solutions, said device being further connected to an analyzer means for obtaining chemical, biochemical, or physical chemical data on said series of solutions. This invention especially relates to an embodiment of the device for obtaining kinetic data on enzyme reactions in solutions. The device is useful because it provides a means for rapidly obtaining chemical, biochemical, or physical chemical data on reactions in solution.

Abstract translation: 本发明涉及稀释和混合装置，其能够稀释第一溶液以产生与未稀释的第三溶液混合的第二溶液，以可重复地产生独特的一系列组合溶液。 所述一系列组合溶液中的每种溶液可以仅在单一（选择的）反应物的浓度上变化，并且通常，每个连续的溶液变得越来越多地浓缩在所选择的反应物中。 通过采用程序的改进，所述系列中的每个连续溶液可能在所选择的反应物中的浓度越来越少。 本发明还涉及一种自动化系统，其包括连接到步进电机的装置，以便快速且可重复地产生所述一系列解决方案，所述装置进一步连接到用于获得所述系列上的化学，生物化学或物理化学数据的分析装置 的解决方案。 本发明特别涉及用于获得溶液中酶反应动力学数据的装置的实施方案。 该设备是有用的，因为它提供了快速获得溶液中反应的化学，生物化学或物理化学数据的手段。

公开(公告)号：WO1988000705A1

公开(公告)日：1988-01-28

申请号：PCT/US1987001621

申请日：1987-07-08

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  MEYER, Richard, C. ,  GREENE, Malbone, W.

IPC: G01N35/00

CPC classification number: B01L7/00

Abstract: A temperature control apparatus for controlling the temperature of a plurality of cuvettes (32) consisting of an annular sealed chamber (20) containing a refrigerant (120), means (18) fixed to the sealed chamber (20) for receiving the sample cuvettes (32), a heater (44, 76) in thermal contact with the sealed chamber, and a temperature sensor (52, 78) in thermal contact with the sealed chamber (20). The sealed chamber (20) may include a plurality of thermally conductive posts (28) fixed to the chamber (20), the spacing between adjacent ones of the posts (28) being adapted to receive the sample cuvettes (32).