公开(公告)号：WO2019089088A1

公开(公告)日：2019-05-09

申请号：PCT/US2018/036622

申请日：2018-06-08

Applicant: FLUIDIGM CANADA INC. ,  CHANG, Qing ,  ALLO, Bedilu ,  LUO, Xudong ,  BOUZEKRI, Alexander

Inventor： CHANG, Qing ,  ALLO, Bedilu ,  LUO, Xudong ,  BOUZEKRI, Alexander ,  ORNATSKY, Olga

IPC: C07K1/13 ,  C12N15/10 ,  C12Q1/68 ,  G01N33/68

Abstract: The present disclosure relates to reagents and their use for elemental imaging mass spectrometry of biological samples.

公开(公告)号：WO2017004203A1

公开(公告)日：2017-01-05

申请号：PCT/US2016/040095

申请日：2016-06-29

Applicant: FLUIDIGM CANADA INC.

Inventor： ORNATSKY, Olga

IPC: C07H19/00 ,  C07H21/02 ,  C07H21/00

CPC classification number: G01N33/6851 ,  C07H21/00 ,  C07H21/02 ,  C12N2310/3145 ,  C12N2310/315 ,  C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6872 ,  C12Q2565/627 ,  C12Q2600/16

Abstract: In certain embodiments, a method of detection includes providing cells labeled with a first element tag bound to a target RNA sequence and a second element tag bound to a target protein. The method may further include ionizing a cell, or a portion thereof, to produce an ionized first and second element tag, and simultaneously detecting the ionized first and second element tag. The ionization may be performed by an inductively coupled plasma (ICP) torch. The method may further comprise nebulizing the cells in suspension prior to ionizing. In certain embodiments, the ionized first and second element tag may be detected by time-of-flight mass spectrometry. Additional methods, and systems and reagents for simultaneous detection of RNA and protein by mass spectrometry are also described.

Abstract translation: 在某些实施方案中，检测方法包括提供标记有与靶RNA序列结合的第一元件标签的细胞和与靶蛋白结合的第二元件标签。 该方法还可以包括电离细胞或其一部分，以产生电离的第一和第二元件标签，同时检测电离的第一和第二元件标签。 电离可以通过电感耦合等离子体（ICP）手电筒进行。 该方法还可以包括在电离之前将悬浮液中的细胞雾化。 在某些实施方案中，可以通过飞行时间质谱法检测电离的第一和第二元件标签。 还描述了用于通过质谱法同时检测RNA和蛋白质的其它方法和系统和试剂。

公开(公告)号：WO2016109825A1

公开(公告)日：2016-07-07

申请号：PCT/US2015/068330

申请日：2015-12-31

Applicant: FLUIDIGM CANADA INC.

Inventor： LOBODA, Alexander V.

IPC: C12M1/34 ,  G01N35/10 ,  H01J49/04

CPC classification number: C12M25/00 ,  G01N1/286 ,  G01N33/4833 ,  G01N2001/045 ,  G01N2001/2886 ,  H01J49/0027 ,  H01J49/04

Abstract: Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer.

Abstract translation: 公开了将生物样品递送到质谱仪的ICP源的装置和方法。 生物材料被布置在载体上的多个离散位置上。 多个离散部位构造成保留生物材料并在施加能量时释放生物材料。 载体位于气体管道附近，并且在从离散位置释放时，生物材料被夹带在气流中，其将生物材料的离散部分通过导管输送到IC​​P源以通过质量细胞计量分析。 该装置和方法可以向质谱仪提供生物材料的分立部分的连续流。

公开(公告)号：WO2015077884A1

公开(公告)日：2015-06-04

申请号：PCT/CA2014/051131

申请日：2014-11-26

Applicant: FLUIDIGM CANADA INC.

Inventor： ORNATSKY, Olga

IPC: C12Q1/37 ,  C07K1/13 ,  C40B70/00 ,  G01N33/48

CPC classification number: G01N33/6848 ,  C07K1/13 ,  C12Q1/37 ,  G01N33/58 ,  G01N2333/95 ,  G01N2458/15

Abstract: The present invention generally relates to methods for the detection of enzymes using elemental analysis.

Abstract translation: 本发明一般涉及使用元素分析检测酶的方法。

公开(公告)号：WO2014063246A1

公开(公告)日：2014-05-01

申请号：PCT/CA2013/050797

申请日：2013-10-22

Applicant: FLUIDIGM CANADA INC.

Inventor： TANNER, Scott

IPC: G01N27/00 ,  C12M1/34 ,  G01N33/483 ,  G01N33/58 ,  C40B20/08 ,  C40B70/00 ,  H01J49/40

CPC classification number: G01N27/626 ,  G01N1/04 ,  G01N2001/045 ,  H01J49/0031 ,  H01J49/0418

Abstract: In a mass cytometer system, a tissue sample labeled with multiple metal tags is supported on an encoded substrate for distribution profile mapping by laser ablation. Groups of elemental ions from each plume generated by each laser pulse are detected by the mass cytometer and the data is mapped according to the encoded substrate. This configuration allows for the production of a 3-dimentional distribution profile of the multiple metal tags in the tissue sample.

Abstract translation: 在质谱仪系统中，通过激光烧蚀在经编码的衬底上支持用多个金属标签标记的组织样品用于分布轮廓图。 通过质谱仪检测由每个激光脉冲产生的每个羽流的元素离子组，并且根据编码的底物映射数据。 这种构造允许生成组织样品中多个金属标签的3维分布轮廓。

公开(公告)号：ES3000669T3

公开(公告)日：2025-03-03

申请号：ES15876373

申请日：2015-12-31

Applicant: FLUIDIGM CANADA INC

Inventor： LOBODA ALEXANDER V

IPC: H01J49/04 ,  G01N1/04 ,  G01N1/28 ,  G01N33/483

Abstract: Se describen aparatos y métodos para suministrar muestras biológicas a una fuente de ICP de un citómetro de masas. El material biológico se dispone en una pluralidad de sitios discretos sobre un portador. La pluralidad de sitios discretos están configurados para retener material biológico y liberar el material biológico tras la aplicación de energía. El portador se coloca en proximidad a un conducto de gas y, tras la liberación desde los sitios discretos, el material biológico queda atrapado en un flujo de gas, que suministra porciones discretas de material biológico a través del conducto a la fuente de ICP para su análisis mediante citometría de masas. El aparato y los métodos pueden proporcionar una corriente continua de porciones discretas de material biológico a un citómetro de masas. (Traducción automática con Google Translate, sin valor legal)

公开(公告)号：CA3145861A1

公开(公告)日：2021-02-11

申请号：CA3145861

申请日：2020-08-07

Applicant: FLUIDIGM CANADA INC ,  GOVERNING COUNCIL UNIV TORONTO

Inventor： LIU PENG ,  MAJONIS DANIEL ,  BARANOV VLADIMIR ,  WINNIK MITCHELL A ,  CHO HYUNGJUN

IPC: A61K51/04

Abstract: A new class of mass-tag polymers is provided, which include enriched metal isotopes such as zirconium and hafnium mass tags. The chemistry of these new mass tags are different from that of lanthanide mass tags, and opens up new mass channels that can be used in mass cytometry. These polymers may be used for mass cytometry, therapeutic delivery of a radioactive isotope, or screening of a therapeutic isotope. Aspects include a kit, method of making, and method of using a polymer, isotopic composition, or both. A kit may include a polymer. The polymer may include pendant groups that chelate an enriched isotope, such as zirconium and/or hafnium. The kit may include an isotopic composition comprising an enriched zirconium or hafnium isotope. Polymers may be conjugated to a biologically active material. Aspects may also include making a kit. Aspects include use of a kit, such as for mass cytometry.

公开(公告)号：SG10201901863RA

公开(公告)日：2019-03-28

申请号：SG10201901863R

申请日：2014-05-28

Applicant: FLUIDIGM CANADA INC

Inventor： TANNER SCOTT

Abstract: STRATEGIC DYNAMIC RANGE CONTROL FOR TIME-OF-FLIGHT MASS SPECTROMETRY A mass spectrometer of the type useful in mass cytometry includes an ion detector. A digitizing system for converting analog signals from the ion detector includes two analog-to-digital converters. The analog-to-digital converters are configured to provide an increased dynamic range for a targeted period while limiting the amount of data generated. [Figure 1]

公开(公告)号：CA3032861A1

公开(公告)日：2018-02-08

申请号：CA3032861

申请日：2017-08-02

Applicant: FLUIDIGM CANADA INC

Inventor： LOBODA ALEXANDER V

IPC: H01J49/04

Abstract: The invention relates to methods and devices for analysis of samples using laser ablation imaging mass cytometry and mass spectrometry. The invention provides methods and devices in which individual ablation plumes are distinctively captured and rapidly transferred to the ionization system, followed by analysis by mass spectrometry. A transfer conduit can be used to convey ablation plumes to an ionization system. The transfer conduit can include an asymmetric cone. The transfer conduit can be tapered. A flow sacrificing system can be adapted to divert a part of the sheath flow out a sacrificial outlet while the core of the sheath flow containing ablation plumes enters the ionization system.

公开(公告)号：ES2582169T3

公开(公告)日：2016-09-09

申请号：ES07710634

申请日：2007-02-13

Applicant: FLUIDIGM CANADA INC

Inventor： ORNATSKY OLGA

IPC: C12Q1/68 ,  C12P19/34 ,  C12Q1/00 ,  C40B30/04 ,  C40B70/00 ,  G01N33/483 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/58

Abstract: Un método para el análisis celular en una célula o partícula celular, en donde dicha célula es una célula entera de origen animal, vegetal, bacteriano o fúngico, o dicha partícula celular se selecciona del grupo que consiste en un cromosoma aislado, un núcleo aislado, una mitocondria aislada, un cloroplasto aislado, y un virus aislado, comprendiendo el método: (a) fijar y permeabilizar la célula o la partícula celular; (b) incubar la célula o la partícula celular en una solución de hibridación con una sonda de ácido nucleico específica para un ácido nucleico diana, donde la sonda marcada con una etiqueta elemental única tal que un tipo de dicha sonda marcada con un tipo de dicha etiqueta es distinguible de cualquier otro tipo de dicha sonda marcada con un tipo diferente de dicha etiqueta por análisis elemental ICP-MS; (c) separar la sonda no hibridada de la sonda hibridada al ácido nucleico diana mediante estrictas condiciones de lavado; y (d) analizar la célula o partícula celular mediante ICP-MS para identificar la sonda y cuantificar la sonda unida al ácido nucleico diana, en donde cada elemento marcado comprende un resto químico que incluye un átomo elemental o una multitud de átomos elementales con uno o muchos isótopos unidos a una estructura molecular de soporte, y en donde la etiqueta elemental comprende además unos medios de unión de dicha etiqueta a un sustrato.