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    Production of biotin by expressing S-adenosyl-methionine synthase and second biotin synthesis gene in host cells
    146.
    发明专利
    Production of biotin by expressing S-adenosyl-methionine synthase and second biotin synthesis gene in host cells 未知

    公开(公告)号：DE19806872A1

    公开(公告)日：1999-08-26

    申请号：DE19806872

    申请日：1998-02-19

    Applicant: BASF AG

    Inventor： SCHROEDER HARTWIG

    IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/10 ,  C12N15/54 ,  C12N15/60 ,  C12P17/18 ,  C12R1/19 ,  C12N15/63 ,  C07H21/04 ,  C12N1/00 ,  C07D495/04

    Abstract: Preparation of biotin (I) comprises expressing, in a prokaryotic or eukaryotic host capable of producing (I): (a) an S-adenosyl-methionine synthase sequence (1), of 1155 bp (reproduced); and (b) at least one of the other biotin biosynthesis genes bioS1, 2 or 3 (sequences of 1206 (3), 1215 (5) and 1221 (7) bp, respectively, reproduced). Preparation of biotin (I) comprises expressing, in a prokaryotic or eukaryotic host capable of producing (I): (a) an S-adenosyl-methionine synthase sequence (1), of 1155 bp (reproduced); and (b) at least one of the other biotin biosynthesis genes bioS1, 2 or 3 (sequences of 1206 (3), 1215 (5) and 1221 (7) bp, respectively, reproduced). The transformed cells are cultured and synthesized (I) used directly, after removal of biomass, or after purification. Independent claims are also included for the following: (1) a gene construct containing (1) and at least one of (3), (5) or (7), or their functional variants, analogs or derivatives, linked to either one or more regulatory signals for increased gene and/or protein expression, and/or to their native regulators; (2) organisms containing the construct of (a); and (3) use of the bioS3 gene (7), or its variants, analogs and derivatives, alone or in combination with other biotin synthesis genes, for production of (I).

    Pgro expression units
    149.
    发明专利
    Pgro expression units 未知

    公开(公告)号：IN439CH2008A

    公开(公告)日：2008-09-19

    申请号：IN439CH2008

    申请日：2008-02-21

    Applicant: BASF AG

    Inventor： KROEGER BURKHARD ,  KLOPPROGGE CORINNA ,  HAEFNER STEFAN ,  SCHROEDER HARTWIG ,  ZELDER OSKAR

    IPC: C07K14/34 ,  C12N15/77 ,  C12P13/08

    Abstract: Use of a nucleic acid (I) with promoter activity for transcribing genes, where (I) is (a) a 164 bp sequence (SEQ ID No,:1); (b) a variant of (SEQ ID No.:1) with at least 90% identity and derived by substitution, insertion or deletion of nucleotides; (c) a sequence that hybridizes to (SEQ ID No.:1) under stringent conditions; or (d) a functionally equivalent fragment of (a)-(c), is new. Independent claims are also included for : (1) use of an expression unit (EU), containing (I) and functionally linked to a sequence (X) that ensures translation of RNA, for expressing genes; (2) (I), other than sequence (1), i.e. (Ia), as new compounds; (3) EU that contain (Ia) linked to (X); (4) altering (or producing) the transcription rate of genes in a microorganism, relative to the wild type; (5) expression cassette (EC) comprising at least one EU of (1), at least one other nucleic acid sequence (to be expressed) and optionally additional gene control elements, where at least the first two are linked and the sequence being expressed is heterologous with respect to EU; (6) expression vector (EV) that contains EC; (7) genetically modified microorganisms (GMO) having, for at least one gene, an altered (or induced) transcription rate, relative to the wild type; (8) preparing biosynthetic products by culturing GMO of (7); (9) use of the sequence ggaggga (53) as a ribosome-binding site in expression units that provide expression of genes in Corynebacterium or Brevibacterium; (10) use of the sequence tagagt (52) as a -10 region for expression of genes in Corynebacterium or Brevibacterium; and (11) expression units that contain sequences (52) or (53).

    150.
    发明专利
    未知

    公开(公告)号：BRPI0513227A

    公开(公告)日：2008-04-29

    申请号：BRPI0513227

    申请日：2005-07-16

    Applicant: BASF AG

    Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

    IPC: C12Q1/68 ,  C07K14/34

    Abstract: A specific nucleic acid (I) with promoter activity is used for transcribing genes, where (I) is: (a) a sequence (1) of 178 nucleotides (reproduced); (b) a derivative of (1) with >= 90% identity, formed by substitution, insertion or deletion; (c) a sequence that hybridizes to (1) under stringent conditions; or (d) a functional fragment of (a)-(c). Independent claims are also included for: (1) use of an expression unit (EU), containing and linked to a nucleic acid (NA) that ensures translation of RNA, for expressing genes; (2) (I), except sequence (1) itself, as new compounds and EU containing it; (3) method for altering (or causing) the transcription (or expression) rate of genes in microorganisms relative to the wild type; (4) expression cassette (EC) comprising EU, at least one other functionally linked NA to be expressed and optionally other genetic control elements that are heterologous with respect to EU; (5) expression vector containing EC; (6) genetically modified microorganism (GMM) in which the transcription rate of at least one gene is altered (or caused) relative to the wild type; (7) GMM containing EU and a functionally linked gene to be expressed, where this is heterologous with respect to EU; (8) preparation of biosynthetic products (A) by culturing the GMM of (6) or (7); (9) use of the sequence aggagga (21) as ribosome-binding site in expression units for expressing genes in Corynebacterium or Brevibacterium; (10) use of the sequences ttaatt (19) or taagct (20) as -10 regions in EU for expressing genes in Corynebacterium or Brevibacterium; and (11) EU that contain (21) or at least one of (19) or (20).

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