公开(公告)号：WO1988010429A1

公开(公告)日：1988-12-29

申请号：PCT/US1988002066

申请日：1988-06-16

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  SEATON, Charles, Bret

IPC: G01N33/70

CPC classification number: G01N33/70 ,  Y10T436/147777 ,  Y10T436/25125 ,  Y10T436/2525

Abstract: A cold stable liquid reagent system suitable for use in a Jaffe method for the determination of creatinine comprises: a) a homogeneous liquid picric acid component comprising picric acid in an amount of no more than about 35 mM, such that the picric acid component remains visibly homogeneous at a temperature between about 2 to about 8°C for 30 days or more; b) a homogeneous liquid alkaline buffer component comprising: i) an alkaline material in sufficient amount to maintain the pH of the alkaline buffer component at more than about 13, for controlling the pH of the assay ready reagent formed by combining the picric acid and alkaline buffer components; ii) at least one pH buffer material; iii) a detergent for reducing interference by proteins in the Jaffe method; and iv) borate for reducing interference by carbohydrates in the Jaffe method; wherein the alkaline buffer components contains substantially no metallic cations such that the component remains visibly homogeneous at a temperature between about 2 to about 8°C for 30 days or more. Preferably the alkaline material is tetramethylammonium hydroxide, and the detergent is diethanolamine dodecyl sulfate.

公开(公告)号：WO1988007668A1

公开(公告)日：1988-10-06

申请号：PCT/US1988001046

申请日：1988-03-31

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  HOLMQUIST, Barton ,  SCOPES, Robert, K.

IPC: G01N01/28

CPC classification number: B01F13/0016 ,  B01F3/08 ,  G01N1/38

Abstract: This invention relates to a diluting and mixing device which is capable of diluting a first solution to produce a second solution which is mixed with an undiluted third solution, to reproducibly produce a unique series of combined solutions. Each solution in said series of combined solutions may vary only in the concentration of a single (selected) reactant, and typically, each successive solution becomes increasingly more concentrated in the selected reactant. By employing a modification in procedure, each successive solution in said series may become decreasingly less concentrated in the selected reactant. This invention further relates to an automated system comprising the device connected to a stepping motor so as to rapidly and reproducibly produce said series of solutions, said device being further connected to an analyzer means for obtaining chemical, biochemical, or physical chemical data on said series of solutions. This invention especially relates to an embodiment of the device for obtaining kinetic data on enzyme reactions in solutions. The device is useful because it provides a means for rapidly obtaining chemical, biochemical, or physical chemical data on reactions in solution.

Abstract translation: 本发明涉及稀释和混合装置，其能够稀释第一溶液以产生与未稀释的第三溶液混合的第二溶液，以可重复地产生独特的一系列组合溶液。 所述一系列组合溶液中的每种溶液可以仅在单一（选择的）反应物的浓度上变化，并且通常，每个连续的溶液变得越来越多地浓缩在所选择的反应物中。 通过采用程序的改进，所述系列中的每个连续溶液可能在所选择的反应物中的浓度越来越少。 本发明还涉及一种自动化系统，其包括连接到步进电机的装置，以便快速且可重复地产生所述一系列解决方案，所述装置进一步连接到用于获得所述系列上的化学，生物化学或物理化学数据的分析装置 的解决方案。 本发明特别涉及用于获得溶液中酶反应动力学数据的装置的实施方案。 该设备是有用的，因为它提供了快速获得溶液中反应的化学，生物化学或物理化学数据的手段。

公开(公告)号：WO1988000705A1

公开(公告)日：1988-01-28

申请号：PCT/US1987001621

申请日：1987-07-08

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  MEYER, Richard, C. ,  GREENE, Malbone, W.

IPC: G01N35/00

CPC classification number: B01L7/00

Abstract: A temperature control apparatus for controlling the temperature of a plurality of cuvettes (32) consisting of an annular sealed chamber (20) containing a refrigerant (120), means (18) fixed to the sealed chamber (20) for receiving the sample cuvettes (32), a heater (44, 76) in thermal contact with the sealed chamber, and a temperature sensor (52, 78) in thermal contact with the sealed chamber (20). The sealed chamber (20) may include a plurality of thermally conductive posts (28) fixed to the chamber (20), the spacing between adjacent ones of the posts (28) being adapted to receive the sample cuvettes (32).

公开(公告)号：WO1988000704A1

公开(公告)日：1988-01-28

申请号：PCT/US1987001609

申请日：1987-07-08

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  ZAKOWSKI, Jack, J. ,  CUOMO, Vincent, A. ,  BLANKE, Gordon, C. ,  MEYER, Richard, C.

IPC: G01N35/00

CPC classification number: G01N35/025 ,  Y10T436/111666

Abstract: A method of operating a clinical analyzer wherein the analyzer includes a plurality of reaction locations (30) divided into two groups. Predetermined operations are performed on the first and second groups during corresponding processing cycles. A process that is common to both cycles is performed simultaneously for reaction locations (30) in the first and second groups during only one of the cycles. The other of the cycles can thus accommodate operations unique to particular tests that would not otherwise be available on the analyzer. The common process may be washing of adjacent reaction locations included in the first and second groups.

Abstract translation: 一种操作临床分析仪的方法，其中分析仪包括分成两组的多个反应位置（30）。 在相应的处理周期期间对第一和第二组执行预定的操作。 对于两个循环共同的过程在仅一个循环期间同时对第一组和第二组中的反应位置（30）进行。 因此，其他循环可以适应特定测试所独有的操作，否则分析仪将无法使用这些测试。 常见的方法可能是洗涤包括在第一和第二组中的相邻反应位置。

公开(公告)号：WO1986006004A1

公开(公告)日：1986-10-23

申请号：PCT/US1986000746

申请日：1986-04-07

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  HARRIS, Paul, C. ,  STONE, Linda, J.

IPC: B01L03/00

CPC classification number: B01L3/502

Abstract: A system and method of immunoassay, both including the use of a reaction capsule (14) having a hydrophobic membrane (22) which may be repeatedly wetted and rendered hydrophobic. A pressure differential across the membrane causes liquid flow therethrough to be initiated and the hydrophobic state is then achieved by flowing gas through the membrane. The system includes a turntable (12) supporting a plurality of reaction capsules (14) and eccentric (66) means for agitating the turntable and capsules. The turntable is rotated to position the capsules at various processing stations, including sample introduction (86), reagent introduction (98), wash (100), substrate introduction (130) and read stations (132). A single cylinder two-inlet valve (178) may be used, one inlet (190) connected to liquid (196) and a second inlet (192) connected to a gas source (202), to provide both liquid and gas flow through the membrane.

Abstract translation: 一种免疫测定的系统和方法，包括使用具有疏水膜（22）的反应胶囊（14），所述疏水膜可被反复润湿并变得疏水。 跨过膜的压差导致液体流过其中，并且然后通过使气体流过膜来实现疏水状态。 该系统包括支撑多个反应胶囊（14）的转台（12）和用于搅拌转盘和胶囊的偏心（66）装置。 转盘旋转以将胶囊定位在各种处理站，包括样品引入（86），试剂引入（98），洗涤（100），基底引入（130）和读取站（132）。 可以使用单缸双入口阀（178），连接到液体（196）的一个入口（190）和连接到气体源（202）的第二入口（192），以提供液体和气体流过 膜。

公开(公告)号：WO1985005568A1

公开(公告)日：1985-12-19

申请号：PCT/US1985001032

申请日：1985-05-31

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  SUTTON, John, H., III

IPC: B04B05/04

CPC classification number: B04B5/0421

Abstract: A centrifuge rotor (18) is presented having a pivotal mount (26) for a sample container (10) upwardly offset from the radial plane (A-A') of maximal strength of the rotor (18) permitting a smaller diameter rotor (18) container (10) assembly and reducing exposed surface area of the rotor (18) container assembly.

Abstract translation: 离心机转子（18）具有用于样品容器（10）的枢转安装件（26），该样品容器（10）向上偏离转子（18）的最大强度的径向平面（A-A'），允许较小直径的转子 ）容器（10）组件并减小转子（18）容器组件的暴露表面积。

公开(公告)号：WO1985005052A1

公开(公告)日：1985-11-21

申请号：PCT/US1985000441

申请日：1985-03-18

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  WEDEMEYER, Robert, Carl ,  GIEBELER, Robert, Henry, Jr.

IPC: B04B15/02

CPC classification number: F25B21/02 ,  B04B15/02

Abstract: A thermoelectric temperature control assembly (10) for transferring heat to or from a heat sink (30). A nonconducting substrate (40) is provided with a plurality of mounting openings (44) for receiving the mounting features of a plurality of respective thermoelectric devices (50), (52) and (54). Each mounting openings (44) is internally partitioned so as to form a pair of flexible tongues (48) by which the thermoelectric devices may be clamped to a heat sink (30) to assure a good thermal contact therewith.

公开(公告)号：WO1985002258A1

公开(公告)日：1985-05-23

申请号：PCT/US1984001888

申请日：1984-11-16

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  CRAGLE, Linda, Katherine ,  HARRIS, Paul, C. ,  LEE, Shih-Yun ,  TUNG, Ker, Kong ,  VODIAN, Morton, Alan

IPC: G01N33/53

CPC classification number: G01N33/54393 ,  G01N33/53 ,  G01N33/54306 ,  G01N33/577 ,  Y10S435/81 ,  Y10S435/962 ,  Y10S435/975 ,  Y10S436/805 ,  Y10S436/808 ,  Y10S436/818 ,  Y10S436/819

Abstract: A improved immunoassay for assaying an antigenic substance (Ag) in a fluid. The immunoassay is the type which comprises contacting the fluid with at least one first entity selected from a group consisting of an antibody (Ab) to the Ag, a soluble, labeled antibody (L-Aba) to the Ag, and an antibody (Abb) to the Ag bound to a solid support (SC). The immunoassay is characterized in that the fluid is contacted with at least one additional entity selected from a group consisting of at least one different type of soluble, labeled antibody (L-Abc) to the Ag, at least one different type of antibody (Abd) bound to a solid carrier (SC1), and at least one different type of antibody (Abe) to the Ag. The SC1 is selected from a group consisting of SC, at least one different solid carrier (SC2), and mixtures thereof (SC and SC2). Each type of L-Abc, Abd-SC1, and Abe has a lower average affinity constant (K) for Ag than each respective K of L-Aba, Abb-SC and Abe; and the additional entity is present in an amount sufficient to avoid a hook effect. Also, an improved reagent of the type comprising at least one first entity selected from a group consisting of L-Aba, Abb-SC, and Ab. The reagent is characterized in that it further comprises at least one additional entity selected from a group consisting of at least one different type of L-Abc and at least one different type Abd-SC1, and at least one different type of Abe. Ab, L-Aba, Abb-SC, L-Abc, Abd-SC1, and Abe are as defined above.

Abstract translation: 用于测定流体中的抗原物质（Ag）的改进的免疫测定。 免疫测定法是将流体与至少一种选自Ag抗体（Ab），Ag的可溶性标记抗体（L-Aba）和抗体（Abb ）到与固体支持物（SC）结合的Ag。 免疫测定的特征在于使流体与至少一种选自由至少一种不同类型的可溶性标记抗体（L-Abc）至Ag，至少一种不同类型的抗体（Abd）的另外的实体接触 ）与固体载体（SC1）结合，并且至少一种不同类型的抗体（Abe）与Ag结合。 SC1选自SC，至少一种不同的固体载体（SC2）及其混合物（SC和SC2）。 每种类型的L-Abc，Abd-SC1和Abe对于Ag的平均亲和常数（K）低于L-Aba，Abb-SC和Abe的各自的K; 并且额外的实体以足以避免钩效应的量存在。 此外，还包括一种包含至少一种选自由L-Aba，Abb-SC和Ab组成的组的第一实体的改良试剂。 该试剂的特征在于，其还包含至少一种选自至少一种不同类型的L-Abc和至少一种不同类型的Abd-SC1和至少一种不同类型的Abe的附加实体。 Ab，L-Aba，Abb-SC，L-Abc，Abd-SC1和Abe如上所定义。

公开(公告)号：WO1985001348A1

公开(公告)日：1985-03-28

申请号：PCT/US1984001506

申请日：1984-09-19

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  CHIPMAN, Russell, A. ,  OBREMSKI, Robert, J. ,  BROWN, Christopher, W.

IPC: G01N21/27

CPC classification number: G01N21/274 ,  G01J3/42

Abstract: A multicomponent quantitative analytical method and apparatus wherein the method includes the steps of and the apparatus is capable of performing steps of obtaining a plurality of calibration spectra, transforming the calibration spectra using a transform with orthogonal basis vectors, obtaining a calibration matrix relating the transform spectra to concentrations of analytes in the calibration samples, obtaining a spectrum for an unknown sample, transforming the unknown sample spectrum, and relating the transformed unknown sample spectrum to the calibration matrix to thereby determine the concentration of analytes in the unknown sample.

公开(公告)号：WO1985000708A1

公开(公告)日：1985-02-14

申请号：PCT/US1984001166

申请日：1984-07-23

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  MEYER, Richard, C. ,  KAN, Peter

IPC: H02P08/00

CPC classification number: H02P8/165 ,  H02P8/32

Abstract: The method includes varying the width of the high level drive impulses of a bi-level drive system based on the desired speed to be achieved by the stepper motor. A microprocessor is used to vary the pulse width.

Abstract translation: 该方法包括基于由步进电机实现的期望速度来改变双级驱动系统的高电平驱动脉冲的宽度。 微处理器用于改变脉冲宽度。