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公开(公告)号：US20160139141A1
公开(公告)日：2016-05-19
申请号：US14940951
申请日：2015-11-13
Applicant: Fluidigm Canada Inc.
Inventor： Alexander V. Loboda
IPC: G01N33/68
CPC classification number: G01N33/6848 , G01N33/58 , G01N2458/15
Abstract: Methods of imaging a biological sample by mass cytometry using molecular tagging are disclosed.
Abstract translation: 公开了通过使用分子标记的质谱法对生物样品进行成像的方法。
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公开(公告)号：US20160131635A1
公开(公告)日：2016-05-12
申请号：US15001589
申请日：2016-01-20
Applicant: Fluidigm Canada Inc.
Inventor： Vladimir Baranov
IPC: G01N33/483 , G01N15/10
CPC classification number: G01N15/10 , C12M1/343 , G01N1/04 , G01N33/4833 , G01N33/569 , G01N2001/045 , G01N2015/1006 , G01N2015/1093
Abstract: A combination of mutually exclusive cell-based analytical techniques can be applied to the same group of cells for analysis. The same group of cells can be prepared for analysis by each technique resulting with candidate cells targeted for mass cytometry analysis. This configuration allows for the correlation of the information between each technique to produce a matrix of multi dimension of cellular information with the same group of cells.
Abstract translation: 相互排斥的基于细胞的分析技术的组合可以应用于同一组细胞用于分析。 可以通过用针对质粒细胞分析的候选细胞产生的每种技术来制备相同组的细胞用于分析。 该配置允许每个技术之间的信息的相关性，以产生具有相同组的单元的蜂窝信息的多维矩阵。
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公开(公告)号：US20160123970A1
公开(公告)日：2016-05-05
申请号：US14894287
申请日：2014-05-22
Applicant: FLUIDIGM CANADA INC.
Inventor： Dmitry R. BANDURA , Vladimir I. BARANOV , Alexander V. LOBODA , Olga ORNATSKY , Scott D. TANNER
IPC: G01N33/543 , G01N15/10 , G01N33/532 , G01N27/62
CPC classification number: G01N33/54366 , G01N15/1031 , G01N15/1404 , G01N15/1459 , G01N27/622 , G01N27/624 , G01N33/483 , G01N33/532 , G01N33/54313 , G01N2015/1006 , G01N2458/15
Abstract: The invention relates to methods and devices for molecular cytometry. The molecular cytometer is for use in the analysis of molecular tag labeled particles. In the molecular cytometer, volatile molecular tags attached to the particles, such as cells, are released as groups of molecular tags and ionized by the method of soft ionization to produce corresponding groups of molecular ions. The molecular cytometer has two detectors, one to detect the presence of the groups of molecular ions and the other to detect mobility separated molecular ions. The mobility separated molecular ions are synchronized to correspond with the groups of molecular ions.
Abstract translation: 本发明涉及用于分子细胞计数的方法和装置。 分子光谱仪用于分子标签标记颗粒的分析。 在分子细胞仪中，附着于粒子（如细胞）的挥发性分子标记作为分子标签的基团释放，并通过软电离方法离子化以产生相应的分子离子组。 分子细胞仪有两个检测器，一个用于检测分子离子组的存在，另一个用于检测迁移率分离的分子离子。 迁移率分离的分子离子被同步以对应于分子离子的组。
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公开(公告)号：US09296838B2
公开(公告)日：2016-03-29
申请号：US14659224
申请日：2015-03-16
Applicant: FLUIDIGM CANADA INC.
Inventor： Mitchell A. Winnik , Mark Nitz , Vladimir Baranov , Xudong Lou
IPC: G01N33/544 , G01N33/545 , G01N33/24 , C08F8/32 , C08F8/42 , C08F220/36 , G01N33/532 , G01N33/58 , C08F8/14
CPC classification number: C08F8/32 , C08F8/14 , C08F8/42 , C08F220/36 , C08F220/56 , G01N33/532 , G01N33/57426 , G01N33/58 , Y10T436/24
Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).
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公开(公告)号：US20140323339A1
公开(公告)日：2014-10-30
申请号：US14327948
申请日：2014-07-10
Applicant: FLUIDIGM CANADA INC.
Inventor： Olga Ornatsky
CPC classification number: G01N33/6848 , C12Q1/42 , C12Q1/485 , G01N33/569 , G01N33/58 , G01N33/6842 , G01N2333/91205 , G01N2333/916 , G01N2440/14 , G01N2458/15 , G01N2560/00 , Y10S435/975
Abstract: Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enyzmes
Abstract translation: 提供了涉及翻译后修饰的酶的方法和试剂盒。 该方法采用元素分析法，包括ICP-MS。 这些方法允许通过参与翻译后修饰的酶（包括激酶和磷酸酶enyzmes）方便和准确地分析底物的翻译后修饰
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Patent Agency Ranking
公开(公告)号：US11776801B2
公开(公告)日：2023-10-03
申请号：US17470769
申请日：2021-09-09
Applicant: Fluidigm Canada Inc.
Inventor： Alexander Loboda , Raymond Jong , Michael Sullivan , Serguei Vorobiev , Robert Rotenberg , Emil D. Stratulativ , Maxim Voronov , Mark Armstrong
CPC classification number: H01J49/105 , H01J49/0027 , H01J49/0463 , H05H1/2406 , H05H1/30
Abstract: Inductively coupled plasma (ICP) analyzers use an ICP torch to generate a plasma in which a sample is atomized an ionized. Analysis of the atomic ions can be performed by atomic analysis, such as mass spectrometry (MS) or atomic emission spectrometry (AES). Particle based ICP analysis includes analysis of particles such as cells, beads, or laser ablation plumes, by atomizing and ionizing particles in an ICP torch followed by atomic analysis. In mass cytometry, mass tags of particles are analyzed by mass spectrometry, such as by ICP-MS. Systems and methods of the subject application include one or more of: a demountable ICP torch holder assembly, an external ignition device; an ICP load coil comprising an annular fin, particle suspension sample introduction fluidics, and ICP analyzers thereof.
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公开(公告)号：US20230013375A1
公开(公告)日：2023-01-19
申请号：US17756475
申请日：2020-11-25
Applicant: FLUIDIGM CANADA INC.
Inventor： Daaf Sandkuijl , Alexander Loboda , Vincent Yi-Ching Yeh , Ladan Gheiratmand
Abstract: Methods and systems for automated slide handling for imaging applications are described herein. In certain aspects, an automated slide handler may be operatively coupled to a slide hotel and/or one or more imaging systems described herein. The automated slide handler may be a robotic arm with up to 6 degrees of freedom. Automated slide handling may include sample preparation, such as sectioning and staining. Suitable imaging systems include a fluorescence microscope or an imaging mass cytometer. Methods and systems disclosed herein enable high throughput profiling of tissue sections.
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公开(公告)号：US20210356376A1
公开(公告)日：2021-11-18
申请号：US17274252
申请日：2019-09-09
Inventor： Taunia Closson , Vladimir Baranov , Mitchell A. Winnik
Abstract: This disclosure relates to reagents and their use for elemental imaging mass spectrometry of biological samples.
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公开(公告)号：US20210333173A1
公开(公告)日：2021-10-28
申请号：US17274331
申请日：2019-09-10
Applicant: Fluidigm Canada Inc.
Inventor： DAAF SANDKUIJL , Alexander V. Loboda , ADAM CAREW , MINALINI LAKSHMAN
Abstract: This disclosure relates to systems and methods for high speed modulation sample imaging. Disclosed herein are systems and methods for performing imaging mass cytometry, including analysis of labelling atoms by elemental (e.g., atomic) mass spectrometry. Aspects include a sampling system having, and method of using, a femtosecond (fs) laser and/or laser scanning. Alternatively or in addition, aspects include systems and methods for co-registering other imaging modalities with imaging mass cytometry.
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公开(公告)号：US10669358B2
公开(公告)日：2020-06-02
申请号：US16047927
申请日：2018-07-27
Applicant: FLUIDIGM CANADA INC.
Inventor： Mitchell A. Winnik , Mark Nitz , Vladimir Baranov , Xudong Lou
IPC: G01N33/532 , C08F8/42 , C08F8/32 , C08F220/36 , G01N33/58 , C08F8/14 , C08F220/56 , G01N33/574
Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).
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