公开(公告)号：WO2005121864A3

公开(公告)日：2006-02-09

申请号：PCT/US2005020250

申请日：2005-06-07

Applicant: FLUIDIGM CORP ,  UNGER MARC A

Inventor： UNGER MARC A

IPC: B01L3/00 ,  B01L7/00 ,  G01N27/447 ,  G02B21/02 ,  G02B21/16 ,  H01L27/00 ,  C12Q1/00 ,  G01J3/30 ,  G01N21/00 ,  G01N21/64 ,  G01T1/10 ,  H05B33/00

CPC classification number: C12Q1/686 ,  B01J2219/00576 ,  B01J2219/00704 ,  B01L3/5027 ,  B01L7/52 ,  G01N21/64 ,  G01N21/6452 ,  G01N21/6456 ,  G01N21/6486 ,  G01N21/75 ,  G01N21/8483 ,  G01N27/44721 ,  G01N2021/6421 ,  G01N2201/061 ,  G02B21/16 ,  G02B21/36

Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device (205). The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device (205). The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device (205). The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system (210, 212) coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.

Abstract translation: 一种用于从微流体装置（205）成像一个或多个选定的荧光指示的装置。 该装置包括耦合到至少一个微流体装置（205）中的至少一个室的成像路径。 成像路径提供从至少一个微流体装置（205）的至少一个室中的一个或多个样品衍生的一个或多个荧光发射信号的传输。 腔室具有腔室尺寸，腔室尺寸的特征在于与成像路径垂直的实际空间尺寸。 该装置还包括耦合到成像路径的光学透镜系统（210,212）。 光学透镜系统适于透射与腔室相关联的一个或多个荧光信号。

公开(公告)号：WO2004089810A3

公开(公告)日：2004-10-21

申请号：PCT/US2004/010492

申请日：2004-04-05

Applicant: FLUIDIGM CORP. ,  MCBRIDE, Lincoln ,  LUCERO, Michael ,  UNGER, Marc ,  NASSEF, Hany Ramez ,  FACER, Geoffrey

Inventor： MCBRIDE, Lincoln ,  LUCERO, Michael ,  UNGER, Marc ,  NASSEF, Hany Ramez ,  FACER, Geoffrey

IPC: B01L3/02

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

公开(公告)号：WO2004089810A2

公开(公告)日：2004-10-21

申请号：PCT/US2004010492

申请日：2004-04-05

Applicant: FLUIDIGM CORP ,  MCBRIDE LINCOLN ,  LUCERO MICHAEL ,  UNGER MARC ,  NASSEF HANY RAMEZ ,  FACER GEOFFREY

Inventor： MCBRIDE LINCOLN ,  LUCERO MICHAEL ,  UNGER MARC ,  NASSEF HANY RAMEZ ,  FACER GEOFFREY

IPC: B01L3/00 ,  B01L3/02 ,  B01L7/00 ,  B01L99/00 ,  B81B20060101 ,  C12Q1/68 ,  G01N1/10 ,  B81B

CPC classification number: B01L3/00 ,  B01L3/5027 ,  B01L3/502723 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/142 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0638 ,  C12Q1/686

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

Abstract translation: 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经预先沉积。 一旦将样品引入反应部位，试剂就会悬浮。 该装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。

公开(公告)号：WO02081935A8

公开(公告)日：2004-10-21

申请号：PCT/US0145294

申请日：2001-11-30

Applicant: FLUIDIGM CORP ,  FERNANDES DAVID N ,  CHOU HOU-PU ,  UNGER MARC A

Inventor： FERNANDES DAVID N ,  CHOU HOU-PU ,  UNGER MARC A

IPC: B01L3/00 ,  F15C3/04 ,  F15C5/00 ,  F16K99/00 ,  G06F17/50

CPC classification number: F15C3/00 ,  A61M2206/22 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0621 ,  B01L2200/14 ,  B01L2300/123 ,  B01L2400/0487 ,  B01L2400/0638 ,  B01L2400/0655 ,  F15C3/04 ,  F16K99/0001 ,  F16K99/0009 ,  F16K99/0015 ,  F16K99/0034 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/008 ,  F16K2099/0082 ,  F16K2099/0084 ,  G06F17/509 ,  G06F2217/16 ,  H01H2029/008 ,  Y10T137/206 ,  Y10T137/2202 ,  Y10T137/2218 ,  Y10T137/2224 ,  Y10T137/7879 ,  Y10T137/7891 ,  Y10T137/7892

Abstract: The present invention provides microfabricated fluidic systems and methods. Microfabricated fluidic devices of the present invention include switches (232, 233) that can be opened and closed to allow or block the flow of fluid through a channel in response to the pressure level in a gate of the switch. The microfabricated fluidic switches may be coupled together to perform logic functions and Boolean algebra, such as inverters, AND gates, NAND, gates, NOR gates, and OR gates. The logic gates may be coupled together to form flip-flops that latch signals. The present invention also includes microfabricated fluidic pressure multipliers that increase the pressure (P2) in a second chamber relative to a first chamber. Microfabricated fluidic devices of the present invention also include pressure sources (560). A pressure source of the present includes a pump coupled to a reservoir through unidirectional valves (500). The pressure source may be high pressure source or a low pressure source. Microfabricated fluidic devices of the present invention may also include devices that perform analog functions such as switching regulator.

Abstract translation: 本发明提供微制造流体系统和方法。 本发明的微型流体装置包括可以打开和关闭的开关（232,233），以响应于开关的门中的压力水平来允许或阻止流体流过通道的流动。 微制造的流体开关可以耦合在一起以执行逻辑功能和布尔代数，例如反相器，与门，NAND，门，NOR门和或门。 逻辑门可以耦合在一起以形成锁存信号的触发器。 本发明还包括相对于第一腔室增加第二腔室中的压力（P2）的微制式流体压力倍增器。 本发明的微型流体装置还包括压力源（560）。 本发明的压力源包括通过单向阀（500）联接到储存器的泵。 压力源可以是高压源或低压源。 本发明的微型流体装置还可以包括执行诸如开关调节器的模拟功能的装置。

公开(公告)号：WO2012162267A3

公开(公告)日：2014-05-15

申请号：PCT/US2012038894

申请日：2012-05-21

Applicant: FLUIDIGM CORP ,  ANDERSON MEGAN ,  CHEN PEILIN ,  FOWLER BRIAN ,  JONES ROBERT C ,  KAPER FIONA ,  LEBOFSKY RONALD ,  MAY ANDREW

Inventor： ANDERSON MEGAN ,  CHEN PEILIN ,  FOWLER BRIAN ,  JONES ROBERT C ,  KAPER FIONA ,  LEBOFSKY RONALD ,  MAY ANDREW

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6855 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/65 ,  C12N15/66 ,  C12Q1/686 ,  C12Q1/6874

Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

Abstract translation: 本文所描述的方法可用于掺入一个或多个衔接子和/或核苷酸标签和/或条形码核苷酸序列，一个或多个靶核苷酸序列。 在具体实施方案中，产生具有适配器（例如适合用于高通量DNA测序）的核酸片段。 在其它实施方案中，关于反应混合物的信息被编码成反应产物。 本文还描述了可用于扩增一种或多种靶核酸以准备应用如双向核酸测序的方法和试剂盒。 在具体实施方案中，本发明的方法还需要进行双向DNA测序。 本文还描述了通过引物延伸来编码和检测和/或定量等位基因的方法。

公开(公告)号：WO2012054933A3

公开(公告)日：2012-06-28

申请号：PCT/US2011057536

申请日：2011-10-24

Applicant: FLUIDIGM CORP ,  LIVAK KENNETH ,  WEST JASON A A ,  JONES ROBERT C

Inventor： LIVAK KENNETH ,  WEST JASON A A ,  JONES ROBERT C

IPC: C12P19/34

CPC classification number: C12Q1/682 ,  C12Q1/6876 ,  C12Q2600/178 ,  C12Q2525/155 ,  C12Q2525/307 ,  C12Q2537/161

Abstract: Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5' to the target sequence, and a second probe-binding sequence 3' to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, said first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.

Abstract translation: 提供了用于检测样品中靶多核苷酸的存在的试剂和方法。 一方面，通过扩增靶核酸序列以产生包含靶序列的扩增产物，与靶序列的第一探针结合序列5'和第二探针结合序列3来产生标记的扩增产物的方法 '到靶序列，从而产生扩增产物; 并且将第一检测探针与扩增产物杂交，所述第一检测探针包括与第一探针结合序列杂交的第一片段和与第二探针结合序列杂交的第二片段，从而产生标记的扩增产物 。

公开(公告)号：WO2011142836A9

公开(公告)日：2012-01-05

申请号：PCT/US2011000887

申请日：2011-05-16

Applicant: FLUIDIGM CORP ,  JONES ROBERT C ,  LIVAK KENNETH J ,  MAY ANDREW ,  MIR ALAIN ,  PIEPRZYK MARTIN ,  QIN JIAN ,  RAMAKRISHNAN RAMESH ,  SPURGEON SANDRA ,  WANG JUN ,  ZIMMERMANN BERNHARD G

Inventor： JONES ROBERT C ,  LIVAK KENNETH J ,  MAY ANDREW ,  MIR ALAIN ,  PIEPRZYK MARTIN ,  QIN JIAN ,  RAMAKRISHNAN RAMESH ,  SPURGEON SANDRA ,  WANG JUN ,  ZIMMERMANN BERNHARD G

IPC: C12Q1/68 ,  G01N33/52 ,  G06F19/18

CPC classification number: C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6858 ,  G01N33/542 ,  G01N33/582 ,  G01N33/6893 ,  G01N2800/368 ,  C12Q2521/301 ,  C12Q2521/501 ,  C12Q2525/155 ,  C12Q2535/131 ,  C12Q2537/143 ,  C12Q2537/16 ,  C12Q2563/173

Abstract: The present invention provides amplification-based methods for detection of genotype, mutations, and/or aneuploidy. These methods have broad applicability, but are particularly well-suited to detecting and quantifying target nucleic acids in free fetal DNA present in a maternal bodily fluid sample.

Abstract translation: 本发明提供了用于检测基因型，突变和/或非整倍性的基于扩增的方法。 这些方法具有广泛的适用性，但特别适合于检测和定量存在于母体液样品中的游离胎儿DNA中的靶核酸。

公开(公告)号：WO2010088288A2

公开(公告)日：2010-08-05

申请号：PCT/US2010022258

申请日：2010-01-27

Applicant: FLUIDIGM CORP ,  DUBE SIMANT ,  MIR ALAIN ,  RAMAKRISHNAN RAMESH ,  WEAVER LESLEY SUZANNE ,  ZIMMERMANN BERNHARD G

Inventor： DUBE SIMANT ,  MIR ALAIN ,  RAMAKRISHNAN RAMESH ,  WEAVER LESLEY SUZANNE ,  ZIMMERMANN BERNHARD G

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6853 ,  C12Q1/6809 ,  C12Q1/6886 ,  C12Q2600/16 ,  C12Q2561/113 ,  C12Q2545/10 ,  C12Q2527/107

Abstract: The present invention provides for determining relative copy number difference for one or more target nucleic acid sequences between a test sample and a reference sample or reference value derived therefrom. The methods facilitate the detection of copy number differences less than 1.5-fold.

Abstract translation: 本发明提供了确定测试样品和参考样品之间的一个或多个目标核酸序列的相对拷贝数差异或其衍生的参考值。 该方法有助于检测小于1.5倍的拷贝数差异。

公开(公告)号：WO2008141183A2

公开(公告)日：2008-11-20

申请号：PCT/US2008063247

申请日：2008-05-09

Applicant: FLUIDIGM CORP ,  FOWLER BRIAN ,  MAY ANDREW

Inventor： FOWLER BRIAN ,  MAY ANDREW

IPC: G01T1/16

CPC classification number: G01N23/20025

Abstract: An integrated fluidic circuit includes a substrate layer and a first structure coupled to the substrate layer and including a plurality of channels. The first structure is configured to provide for flow of one or more materials through the plurality of channels. The integrated fluidic circuit also includes a second structure coupled to the substrate layer. The second structure includes a plurality of control channels configured to receive an actuation pressure. The integrated fluidic circuit is characterized by a thickness of less than 1.5 mm.

Abstract translation: 集成流体回路包括衬底层和耦合到衬底层并且包括多个通道的第一结构。 第一结构被配置为提供通过多个通道的一种或多种材料的流动。 集成流体回路还包括耦合到衬底层的第二结构。 第二结构包括被配置为接收致动压力的多个控制通道。 集成流体回路的特征在于厚度小于1.5mm。

公开(公告)号：WO2008043046A3

公开(公告)日：2008-08-07

申请号：PCT/US2007080489

申请日：2007-10-04

Applicant: FLUIDIGM CORP ,  WANG JING ,  NASSEF HANY R

Inventor： WANG JING ,  NASSEF HANY R

IPC: F16K1/00 ,  F16K15/00

CPC classification number: F16K99/0026 ,  B01L3/502738 ,  B01L2200/12 ,  B01L2300/0887 ,  B01L2400/0605 ,  B01L2400/0638 ,  F16K99/0001 ,  F16K99/0057 ,  F16K2099/0078 ,  F16K2099/008

Abstract: A microfluidic check valve and a method of using the check valve In microfluidic devices includes a check valve comprising two stacked chambers that are separated by a pore-containing membrane. The membrane is composed of an elastomeric material and can be configured in normally open or normally closed state. The normally open check valve can be implemented so that the degree of back pressure necessary to close the valve can be set.

Abstract translation: 微流体止回阀和使用止回阀的方法在微流体装置中包括止回阀，其包括由含孔膜隔开的两个堆叠室。 膜由弹性体材料构成，可以配置成常开或常闭状态。 可以实现常开止回阀，使得可以设定关闭阀所需的背压程度。