公开(公告)号：WO2009023547A2

公开(公告)日：2009-02-19

申请号：PCT/US2008/072581

申请日：2008-08-08

Applicant: ARCXIS BIOTECHNOLOGIES ,  THOMPSON, Jesse ,  WEST, Jason, A., A.

Inventor： THOMPSON, Jesse ,  WEST, Jason, A., A.

IPC: B01L3/00 ,  C12M1/34 ,  C12Q1/00

CPC classification number: B01L3/502707 ,  B01J19/0046 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00722 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0887 ,  B01L2300/12 ,  B29L2031/756 ,  G01N33/54386

Abstract: Provided are microfluidic devices and methods for fabricating and bonding such devices. Also provided are kits for analyzing analyte-containing samples and for lysing cells.

Abstract translation: 提供了用于制造和结合这种装置的微流体装置和方法。 还提供了用于分析含分析物样品和裂解细胞的试剂盒。

公开(公告)号：WO2006036757A2

公开(公告)日：2006-04-06

申请号：PCT/US2005034045

申请日：2005-09-23

Applicant: SANDIA NAT LAB ,  WEST JASON A A ,  HUKARI KYLE W ,  PATEL KAMLESH D ,  PETERSON KENNETH A

Inventor： WEST JASON A A ,  HUKARI KYLE W ,  PATEL KAMLESH D ,  PETERSON KENNETH A

CPC classification number: B01L3/5027 ,  B01L7/00 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1827 ,  C12M47/06 ,  C12M47/20 ,  C12N1/066

Abstract: Devices and methods for thermally lysing of biological material, for example vegetative bacterial cells and bacterial spores, are provided. Hot solution methods for solubilizing bacterial spores are described. Systems for direct analysis are disclosed including thermal lysers coupled to sample preparation stations. Integrated systems capable of performing sample lysis, labeling and protein fingerprint analysis of biological material, for example, vegetative bacterial cells, bacterial spores and viruses are provided.

Abstract translation: 提供了用于热裂解生物材料的装置和方法，例如营养细菌细菌和细菌孢子。 描述了溶解细菌孢子的热溶解方法。 公开了用于直接分析的系统，包括耦合到样品制备站的热裂解器。 提供能够对生物材料进行样品裂解，标记和蛋白质指纹分析的集成系统，例如营养细菌细菌，细菌孢子和病毒。

公开(公告)号：WO2009023547A3

公开(公告)日：2009-04-16

申请号：PCT/US2008072581

申请日：2008-08-08

Applicant: ARCXIS BIOTECHNOLOGIES ,  THOMPSON JESSE ,  WEST JASON A A

Inventor： THOMPSON JESSE ,  WEST JASON A A

IPC: B01L3/00 ,  C12M1/34 ,  C12Q1/00

CPC classification number: B01L3/502707 ,  B01J19/0046 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00722 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0887 ,  B01L2300/12 ,  B29L2031/756 ,  G01N33/54386

Abstract: Provided are microfluidic devices and methods for fabricating and bonding such devices. Also provided are kits for analyzing analyte-containing samples and for lysing cells.

Abstract translation: 提供了用于制造和结合这种装置的微流体装置和方法。 还提供了用于分析含分析物的样品和裂解细胞的试剂盒。

公开(公告)号：WO2012054933A2

公开(公告)日：2012-04-26

申请号：PCT/US2011/057536

申请日：2011-10-24

Applicant: FLUIDIGM CORPORATION ,  LIVAK, Kenneth ,  WEST, Jason A., A. ,  JONES, Robert, C.

Inventor： LIVAK, Kenneth ,  WEST, Jason A., A. ,  JONES, Robert, C.

IPC: C12Q1/68

CPC classification number: C12Q1/682 ,  C12Q1/6876 ,  C12Q2600/178 ,  C12Q2525/155 ,  C12Q2525/307 ,  C12Q2537/161

Abstract: Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5' to the target sequence, and a second probe-binding sequence 3' to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, said first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.

Abstract translation: 提供了用于检测样品中靶多核苷酸存在的试剂和方法。 一方面，通过扩增靶核酸序列以产生包含靶序列，与靶序列5'的第一探针结合序列和第二探针结合序列3的扩增产物来产生标记的扩增产物的方法 '到靶序列，从而产生扩增产物; 并将第一检测探针与扩增产物杂交，所述第一检测探针包含与第一探针结合序列杂交的第一片段和与第二探针结合序列杂交的第二片段，从而产生标记的扩增产物

公开(公告)号：WO2006036757A3

公开(公告)日：2006-04-06

申请号：PCT/US2005/034045

申请日：2005-09-23

Applicant: SANDIA NATIONAL LABORATORIES ,  WEST, Jason, A., A. ,  HUKARI, Kyle, W. ,  PATEL, Kamlesh, D. ,  PETERSON, Kenneth, A.

Inventor： WEST, Jason, A., A. ,  HUKARI, Kyle, W. ,  PATEL, Kamlesh, D. ,  PETERSON, Kenneth, A.

IPC: C12M1/33 ,  B01L3/00 ,  C12N1/06

Abstract: Devices (100) and methods for thermally lysing of biological material, for example vegetative bacterial cells and bacterial spores, are provided. Hot solution methods for solubilizing bacterial spores are described. Systems for direct analysis are disclosed including thermal lysers (100) coupled to sample preparation stations. Integrated systems capable of performing sample lysis, labeling and protein fingerprint analysis of biological material, for example, vegetative bacterial cells, bacterial spores and viruses are provided.

公开(公告)号：WO2012054933A3

公开(公告)日：2012-06-28

申请号：PCT/US2011057536

申请日：2011-10-24

Applicant: FLUIDIGM CORP ,  LIVAK KENNETH ,  WEST JASON A A ,  JONES ROBERT C

Inventor： LIVAK KENNETH ,  WEST JASON A A ,  JONES ROBERT C

IPC: C12P19/34

CPC classification number: C12Q1/682 ,  C12Q1/6876 ,  C12Q2600/178 ,  C12Q2525/155 ,  C12Q2525/307 ,  C12Q2537/161

Abstract: Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5' to the target sequence, and a second probe-binding sequence 3' to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, said first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.

Abstract translation: 提供了用于检测样品中靶多核苷酸的存在的试剂和方法。 一方面，通过扩增靶核酸序列以产生包含靶序列的扩增产物，与靶序列的第一探针结合序列5'和第二探针结合序列3来产生标记的扩增产物的方法 '到靶序列，从而产生扩增产物; 并且将第一检测探针与扩增产物杂交，所述第一检测探针包括与第一探针结合序列杂交的第一片段和与第二探针结合序列杂交的第二片段，从而产生标记的扩增产物 。