公开(公告)号：US20190352674A1

公开(公告)日：2019-11-21

申请号：US16399145

申请日：2019-04-30

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Alexander Brett FOSTER ,  Cristina Serrano AMATRIAIN ,  Gary J. Smith ,  Paul Sheldon Pearlman ,  Mark Paul Taylor ,  Jonathan Combe ,  Daniel Bawdon

IPC: C12P7/04 ,  C12N1/20 ,  C12P7/06 ,  C12P7/42 ,  C12N9/10 ,  C12N9/88 ,  C12N9/04

Abstract: Provided herein are methods for increasing the yield of an extracellular product synthesized by an organism cultured in a continuous aerobic fermentation system. The extracellular product yield is increased through the use of an organism modified to decreased production of polyhydroxyalkanoate, to increase production of the extracellular product, and to include promoters that can be inducible in response to nutrient limitation conditions. The extracellular product yield is also increased by operating the continuous fermentation system under particular nutrient limitation conditions. Also provided are non-naturally occurring organisms that have been modified for use with the provided methods, and extracellular products made using the provided methods.

公开(公告)号：US20190338377A1

公开(公告)日：2019-11-07

申请号：US16398401

申请日：2019-04-30

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Alexander Brett FOSTER ,  Emilie Sophie FRITSCH

IPC: C12R1/38 ,  C12N9/02 ,  C12N15/68 ,  C12N15/52 ,  C12N9/14 ,  C12P7/16

Abstract: Methods of redirecting carbon flux and increasing C2/C3 or a C4/5/6 carbon chain length carbon-based chemical product yield in an organism, nonnaturally occurring organisms with redirected carbon flux and increased C2/C3 or C4/5/6 carbon chain length carbon-based chemical product yield and methods for using these organisms in production of C2/C3 or C4/5/6 carbon chain length carbon-based chemical products are provided.

公开(公告)号：US20190338375A1

公开(公告)日：2019-11-07

申请号：US16398351

申请日：2019-04-30

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Alexander Brett Foster ,  Jonathan Paul Combe ,  Arghya Barman

IPC: C12R1/38 ,  C12N15/78 ,  C12P7/18 ,  C12N9/14 ,  C12P7/54 ,  C12P7/16

Abstract: Methods for increasing carbon-based chemical product yield in an organism by perturbing redox balance in an organism as well as nonnaturally occurring organisms with perturbed redox balance and methods for their use in producing carbon-based chemical products are provided.

公开(公告)号：US20190316072A1

公开(公告)日：2019-10-17

申请号：US16372106

申请日：2019-04-01

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Gary J. Smith ,  Paul S. Pearlman ,  Gregory S. Kirby

IPC: C12M1/00 ,  C12P7/62 ,  C12M1/34

Abstract: Provided herein are systems and methods for recycling and supplementing off-gas from a gas fed reaction process. The systems and methods are particularly useful for bioprocesses that convert hydrogen gas into one or more biosynthetic products. By maintaining separate hydrogen and oxygen feed gas streams, and forming a recycle gas that introduces a target component of the supply gas to the bioreactor within a target concentration range, the yields, productivities, and safety profiles of the bioprocess can be enhanced.

公开(公告)号：US20190300839A1

公开(公告)日：2019-10-03

申请号：US16372099

申请日：2019-04-01

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Gary J. Smith ,  Paul S. Pearlman ,  Gregory S. Kirby

IPC: C12M1/34

Abstract: The present disclosure provides methods for controlling oxygen concentration during aerobic biosynthesis, e.g., fermentation. The method may comprise feeding an oxygen-containing gas into a vessel including a fermentation feedstock and reacting the fermentation feedstock with the oxygen-containing gas to form a broth including a gaseous phase dispersed within the broth. The gaseous phase may comprise any unreacted oxygen from the oxygen-containing gas. The method further includes reducing the concentration of the unreacted oxygen in the dispersed gaseous phase to less than the limiting oxygen concentration (“LOC”) for flammability before separating the gaseous phase from the fermentation broth. The concentration of the unreacted oxygen in the gaseous phase is reduced by employing oxygen removal schemes or oxygen dilution schemes.

公开(公告)号：US20190300838A1

公开(公告)日：2019-10-03

申请号：US16372092

申请日：2019-04-01

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Gary J. Smith ,  Paul S. Pearlman ,  Gregory S. Kirby

IPC: C12M1/34 ,  C12M1/107 ,  C12M1/00 ,  C12M1/36

Abstract: Disclosed are materials and methods for managing aerobic biosynthesis. The materials include a fermenter system comprising a fermenter, a microorganism provided to the fermenter, and at least two control loops. The methods are directed to measuring and controlling different oxygen concentrations within the fermenter.

公开(公告)号：US20190271014A1

公开(公告)日：2019-09-05

申请号：US16256140

申请日：2019-01-24

Applicant: INVISTA North America S.á.r.l.

Inventor： Alexander Brett Foster ,  Mariusz Kamionka ,  Nadia Fatma Kadi ,  Adriana Leonora Botes ,  Alex Van Eck Conradie

IPC: C12P13/00 ,  C12P17/10 ,  C12P7/44 ,  C12P7/18 ,  C07H19/207 ,  C12P7/42 ,  C07H1/00 ,  C12N9/10

Abstract: This document describes materials and methods for, for example, producing 6-hydroxyhexanoic acid using a β-ketothiolase or synthase and an alcohol O-acetyltransferase to form a 6-acetyloxy-3-oxohexanoyl-CoA intermediate. This document describes biochemical pathways for producing 6-hydroxyhexanoic acid using a β-ketothiolase or synthase and an alcohol O-acetyltransferase to form a 6-acetyloxy-3-oxohexanoyl-CoA intermediate. 6-hydroxyhexanoic acid can be enzymatically converted to adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine or 1,6-hexanediol. This document also describes recombinant hosts producing 6-hydroxyhexanoic acid as well as adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine and 1,6-hexanediol.

公开(公告)号：US20190233853A1

公开(公告)日：2019-08-01

申请号：US16264765

申请日：2019-02-01

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Cristina Serrano Amatriain ,  Alexander Brett Foster ,  Stephen Thomas Cartman

IPC: C12P7/52 ,  C12N9/88 ,  C12N9/02 ,  C12N9/16

CPC classification number: C12P7/52 ,  C12N9/0008 ,  C12N9/16 ,  C12N9/88 ,  C12Y102/01003 ,  C12Y301/03021 ,  C12Y402/0103

Abstract: Methods and materials for the production of beta hydroxy acids, such as 3-hydroxypropanoic acid (3-HP) and/or derivatives thereof and/or compounds related thereto, are provided. Also provided are products produced in accordance with these methods and materials.

公开(公告)号：US20190233851A1

公开(公告)日：2019-08-01

申请号：US16264782

申请日：2019-02-01

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Jonathan KENNEDY ,  Alexander Brett FOSTER

IPC: C12P7/42 ,  C12N9/16 ,  C12N9/02 ,  C12N9/88 ,  C12N9/00 ,  C12N15/52 ,  C12R1/01

CPC classification number: C12P7/42 ,  C12N9/001 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12R1/01 ,  C12Y103/01039 ,  C12Y301/02007 ,  C12Y401/99005 ,  C12Y602/01003

Abstract: Methods and materials for the production of compounds involved in fatty acid metabolism, and/or derivatives thereof and/or compounds related thereto are provided. Also provided are products produced in accordance with the methods and materials of the present invention.

公开(公告)号：US20190218577A1

公开(公告)日：2019-07-18

申请号：US16337481

申请日：2017-09-27

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Stephen Thomas Cartman ,  Alexander Brett Foster ,  Ana Teresa De Santos Brito Mendes Roberts ,  Mark Paul Taylor

IPC: C12P5/00 ,  C12N9/90 ,  C12N9/88 ,  C07C11/12 ,  C08F136/08

CPC classification number: C12P5/007 ,  C07C11/12 ,  C08F136/08 ,  C12N9/88 ,  C12N9/90 ,  C12N15/74 ,  C12N2330/30 ,  C12P5/026 ,  C12R1/01 ,  C12Y402/03027 ,  C12Y503/03002 ,  Y02E50/343

Abstract: Methods and compositions for synthesizing dienes and derivative thereof, such as isoprene, in Cupriavidus necator are provided.