公开(公告)号：WO2007063425A3

公开(公告)日：2007-11-08

申请号：PCT/IB2006003982

申请日：2006-06-15

Applicant: UNIV HEALTH NETWORK ,  KOLIOS MICHAEL ,  CZARNOTA GREGORY J ,  SHERAR MICHAEL D ,  TUNIS ADAM ,  HUNT JOHN W

Inventor： KOLIOS MICHAEL ,  CZARNOTA GREGORY J ,  SHERAR MICHAEL D ,  TUNIS ADAM ,  HUNT JOHN W

IPC: A61B8/00 ,  A61B8/08

CPC classification number: A61B8/08 ,  A61B2090/378 ,  A61F7/00 ,  A61N5/062 ,  A61N5/10

Abstract: A method of detecting cellular damage within a subject comprises transmitting low frequency ultrasound (20MHz or below) into a selected site within the subject wherein the selected site has been exposed to a stress capable of causing cellular death at the selected site. At least a portion of ultrasound backscattered from the ultrasound transmitted into the selected site is received. The received backscattered ultrasound is compared to a control backscatter measurement. An increase or a decrease in intensity or spectral slope of the received backscattered ultrasound when compared to the control backscatter measurement indicates cellular death or damage at the selected site within the subject.

Abstract translation: 检测受试者内的细胞损伤的方法包括将低频超声波（20MHz或更低）发送到受试者内的选定部位，其中所选择的部位已暴露于能够在所选位点引起细胞死亡的应激。 从被发送到所选位置的超声波向后散射的超声波的至少一部分被接收。 将接收的反向散射超声波与控制反向散射测量进行比较。 与控制反向散射测量相比，接收的反向散射超声的强度或光谱斜率的增加或减小表明在受试者内的选定部位处的细胞死亡或损伤。

公开(公告)号：WO2007063425A2

公开(公告)日：2007-06-07

申请号：PCT/IB2006/003982

申请日：2006-06-15

Applicant: UNIVERSITY HEALTH NETWORK ,  KOLIOS, Michael ,  CZARNOTA, Gregory, J. ,  SHERAR, Michael, D. ,  TUNIS, Adam ,  HUNT, John, W.

Inventor： KOLIOS, Michael ,  CZARNOTA, Gregory, J. ,  SHERAR, Michael, D. ,  TUNIS, Adam ,  HUNT, John, W.

CPC classification number: A61B8/08 ,  A61B2090/378 ,  A61F7/00 ,  A61N5/062 ,  A61N5/10

Abstract: A method of detecting cellular damage within a subject comprises transmitting low frequency ultrasound (20MHz or below) into a selected site within the subject wherein the selected site has been exposed to a stress capable of causing cellular death at the selected site. At least a portion of ultrasound backscattered from the ultrasound transmitted into the selected site is received. The received backscattered ultrasound is compared to a control backscatter measurement. An increase or a decrease in intensity or spectral slope of the received backscattered ultrasound when compared to the control backscatter measurement indicates cellular death or damage at the selected site within the subject.

Abstract translation: 检测受试者体内细胞损伤的方法包括将低频超声波（20MHz或以下）发射到受试体内的选定部位，其中所选部位已暴露于能够导致细胞在 选定的网站。 接收从发送到所选位置的超声波反向散射的超声的至少一部分。 将接收到的背散射超声与控制后向散射测量进行比较。 与对照反向散射测量值相比，所接收的反向散射超声波的强度或谱斜率的增加或减少指示受试者内选定部位的细胞死亡或损伤。

公开(公告)号：WO0011468A3

公开(公告)日：2000-06-02

申请号：PCT/CA9900770

申请日：1999-08-19

Applicant: ONTARIO CANCER INST ,  SHERAR MICHAEL D ,  CZARNOTA GREGORY J ,  HUNT JOHN W ,  KOLIOS MICHAEL C

Inventor： SHERAR MICHAEL D ,  CZARNOTA GREGORY J ,  HUNT JOHN W ,  KOLIOS MICHAEL C

IPC: A61B8/08 ,  G01N29/06 ,  G01N29/46 ,  G01S7/52 ,  G01S15/89 ,  A61B8/13

CPC classification number: G01S7/52036 ,  A61B8/08 ,  G01N29/0672 ,  G01N29/46 ,  G01N2291/02854 ,  G01S15/895 ,  G01S15/8956

Abstract: A non-invasive method of monitoring apoptosis in cell culture, ex-vivo tissues and in-vivo tissues using high frequency ultrasound imaging is provided. The method includes the steps of: 1) imaging a selected site of the cell culture or tissues using high frequency (above 20 MHz) ultrasound imaging (before image); 2) exposing the selected site to an apoptosis-inducing stress; 3) imaging the selected site or a portion thereof, using ultrasound imaging at subsequent timed intervals (after image(s)); 4) measuring the signal amplitude of a region of interest of the selected site in the before and after images; 5) comparing the signal amplitude measurements for the regions of interest in the before and after images and determining whether the after image regions exhibit an increase in amplitude as compared to the before image regions which is an indication that apoptosis has begun; and 6) measuring the change in the frequency spectrum of the radiofrequency ultrasound backscatter signal in the region of interest in the before and after images and confirming that apoptosis has begun when the slope of the frequency spectrum has increased.

Abstract translation: 提供了使用高频超声成像监测细胞培养，离体组织和体内组织凋亡的非侵入性方法。 该方法包括以下步骤：1）使用高频（高于20MHz）超声成像（图像之前）成像细胞培养物或组织的选定位点; 2）将选择的位点暴露于凋亡诱导应激; 3）在随后的定时间隔（图像之后）使用超声成像对所选择的位点或其部分进行成像; 4）测量前后图像中所选位置的感兴趣区域的信号幅度; 5）比较前后图像中感兴趣区域的信号幅度测量值，并确定后一图像区域是否与作为凋亡开始指示的先前图像区域之间的振幅增加; 以及6）测量前后图像中感兴趣区域中的射频超声后向散射信号的频谱变化，并确认当频谱的斜率增加时已经开始凋亡。