公开(公告)号：WO1994005983A1

公开(公告)日：1994-03-17

申请号：PCT/US1993008153

申请日：1993-08-30

Applicant: PACKARD INSTRUMENT COMPANY, INC.

Inventor： PACKARD INSTRUMENT COMPANY, INC. ,  VALENTA, Robert, J.

IPC: G01J01/08

CPC classification number: G01T1/40 ,  G01J1/08 ,  G01J1/18 ,  G01N21/76 ,  G01T7/00

Abstract: A normalization system for multi-photodetector single photon counting luminescent measuring systems and a standard for use in the same. The method comprises calculating one or more counting ratios for each photodetector in the single photon counting system and multiplying counts of unknown samples by the inverse of the appropriate counting ratio. A counting ratio for a particular photodetector is calculated by dividing the count of a standard measured by the photodetector by the count of the same standard measured by a reference photodetector. The particular standard developed employs calcium tungstate: lead as a scintillator and radio-active elements as tritium or carbon-14 thymidine as a means of exciting the scintillator. This standard has the advantages of providing a good model of typical luminescence chemistry and producing an emission pattern which is susceptible to single photon counting.

Abstract translation: 用于多光电探测器单光子计数发光测量系统的标准化系统及其使用的标准。 该方法包括计算单个光子计数系统中每个光电探测器的一个或多个计数比，并将未知样本的计数乘以适当计数比的倒数。 通过将由光电检测器测量的标准的计数除以由参考光电检测器测量的相同标准的计数来计算特定光电检测器的计数比。 开发的特定标准是使用钨酸钙：铅作为闪烁体，放射性活性元素作为氚或碳-14胸苷作为激发闪烁体的手段。 该标准具有提供典型发光化学的良好模型并产生易受单光子计数影响的发射图案的优点。

公开(公告)号：WO1991008489A1

公开(公告)日：1991-06-13

申请号：PCT/US1990006646

申请日：1990-11-14

Applicant: PACKARD INSTRUMENT COMPANY, INC.

Inventor： PACKARD INSTRUMENT COMPANY, INC. ,  VAN CAUTER, Gustaaf, C. ,  OSTEN, Donald, E. ,  NEUMANN, Kenneth, E.

IPC: G01N33/573

CPC classification number: G01N33/60 ,  G01N33/542 ,  G01N33/552

Abstract: A new support body (10) for use in a scintillation proximity radioimmunoassay consisting of a scintillation material. The support body (10) of the present invention permits extremely sensitive and specific assays for a broad range of biological and non-biological substances. The support body (10) of the present invention having a ligand (16) coupled to its surface which is capable of selectively biochemically binding to a reactant of interest (11) resulting in a complex (18).

Abstract translation: 一种用于由闪烁材料组成的闪烁近距离放射免疫测定的新的支撑体（10）。 本发明的支撑体（10）允许用于广泛范围的生物和非生物物质的非常灵敏和特异性的测定。 本发明的支撑体（10）具有与其表面偶联的配体（16），其能够选择性地与感兴趣的反应物（11）生物化学结合，导致复合物（18）。

公开(公告)号：WO1993003911A1

公开(公告)日：1993-03-04

申请号：PCT/US1992004465

申请日：1992-05-27

Applicant: PACKARD INSTRUMENT COMPANY, INC.

Inventor： PACKARD INSTRUMENT COMPANY, INC. ,  DANIELS, Leslie, Alan ,  OLSON, Robert, Elmer ,  ZIELINSKI, Stanley, Andrew

IPC: B29D22/00

CPC classification number: B01L3/50825 ,  C08J7/065 ,  G21F5/015 ,  G21Y2002/204 ,  G21Y2002/206 ,  G21Y2004/10 ,  G21Y2004/304

Abstract: A vial (10) for holding a radioactive sample for radiometric analysis which has an outer surface (14) on which a coating comprising a transparent antistatic agent (18) is deposited to prevent the accumulation of electrostatic charges on the vial. The vial (10) is treated to make the vial free of electrostatic charges by immersing the vial (10) and cap (12) with a solution containing the antistatic agent, spraying the vial (10) and cap (12) with a solution containing the antistatic agent or by contacting the vial (10) and cap (12) in a heated air stream with a flexible substrate carrying the antistatic agent.

Abstract translation: 一种用于放置用于放射性分析的放射性样品的小瓶（10），其具有外表面（14），在其上沉积包含透明抗静电剂（18）的涂层，以防止静电电荷积累在小瓶上。 通过将小瓶（10）和盖（12）浸入含有抗静电剂的溶液中来处理小瓶（10）以使小瓶没有静电电荷，用小瓶（10）和帽（12）将含有 或者通过使带有抗静电剂的柔性基底的加热空气流中的小瓶（10）和盖（12）接触。

公开(公告)号：WO1992020448A1

公开(公告)日：1992-11-26

申请号：PCT/US1992002183

申请日：1992-03-17

Applicant: PACKARD INSTRUMENT COMPANY, INC.

Inventor： PACKARD INSTRUMENT COMPANY, INC. ,  STANLEY, Deryl, B.

IPC: B01L03/00

CPC classification number: B01L3/5085

Abstract: A microplate (10) for radiometric analysis having a plurality of sample wells (12) for holding radioactive samples is manufactured from a gas barrier resin which is chemically resistant to hydrocarbon solvents. The resin is typically a rubber modified copolymer which includes at least 50 weight percent of an unsaturated nitrile component and a monomer component which is capable of being copolymerized with the nitrile component. A preferred gas barrier resin is an acrylonitrile-methyl acrylate copolymer. A white pigment, preferably titanium dioxide, is added to the resin to produce an opaque, highly reflective microplate (10).

Abstract translation: 用于放射性分析的微孔板（10）由具有化学耐受烃溶剂的阻气性树脂制成，具有用于保存放射性样品的多个样品孔（12）。 树脂通常是橡胶改性共聚物，其包含至少50重量％的不饱和腈组分和能够与腈组分共聚的单体组分。 优选的阻气性树脂是丙烯腈 - 丙烯酸甲酯共​​聚物。 将白色颜料（优选二氧化钛）加入到树脂中以产生不透明的高反射性微孔板（10）。

公开(公告)号：WO1996004571A1

公开(公告)日：1996-02-15

申请号：PCT/US1995009627

申请日：1995-07-31

Applicant: PACKARD INSTRUMENT COMPANY, INC.

Inventor： PACKARD INSTRUMENT COMPANY, INC. ,  VALENTA, Robert, J.

IPC: G01T01/202

CPC classification number: G01T1/204

Abstract: A scintillation measurement system for measuring optical events produced by scintillators (19) in response to the radioactive decay of a constituent or constituents of a sample (10) to be measured comprises a sample support for positioning a sample in a sample well (11); a bismuth germanate (BGO) scintillation crystal (19), such as Bi4Ge3O12, located adjacent the sample well; a plurality of photodetectors (16, 18) located outside the bismuth germanate crystal (19) for detecting optical events occuring in the sample well (11) or in the bismuth germanate crystal (19) and converting those optical events into electrical pulses; and a pulse analyzing system (40) for receiving the electrical pulses from the photodetectors and determining whether such pulses represent alpha, beta or gamma events. This system can be used with samples containing alpha, beta or gamma emitters, or any combination thereof.

Abstract translation: 用于测量由闪烁体（19）响应待测样品（10）的成分或成分的放射性衰变产生的光学事件的闪烁测量系统包括用于将样品定位在样品阱（11）中的样品支架; 位于样品井附近的锗酸铋（BGO）闪烁晶体（19），如Bi4Ge3O12; 位于锗酸铋晶体（19）外部的多个光电检测器（16,18），用于检测样品阱（11）或锗酸铋晶体（19）中发生的光学事件，并将这些光学事件转换为电脉冲; 以及脉冲分析系统（40），用于从光电检测器接收电脉冲并确定这样的脉冲是否表示α，β或γ事件。 该系统可用于含有α，β或γ发射体的样品，或其任何组合。

公开(公告)号：WO1994006932A1

公开(公告)日：1994-03-31

申请号：PCT/US1993008612

申请日：1993-09-10

Applicant: PACKARD INSTRUMENT COMPANY, INC.

Inventor： PACKARD INSTRUMENT COMPANY, INC. ,  ROELANT, Chris

IPC: C12Q01/06

CPC classification number: G01N33/5014 ,  C12Q1/06 ,  Y10S435/968 ,  Y10S435/975 ,  Y10S436/904 ,  Y10S436/905

Abstract: The present invention provides a process of quantifying the number of viable cells in an aqueous suspension of cells using an energy-emitting non-hazardous probe and a probe-trigger. The process provides quantification data in short periods of time without the use of hazardous materials. A process of the present invention can also be used to quantify negatively charged particle number, assay for cytotoxicity, assay for cell proliferation and assay for cell differentiation. Still further, the present invention provides an assay kit for quantification of cells or negatively charged particles.

Abstract translation: 本发明提供了使用能量发射非危险探针和探针触发剂来定量细胞水性悬浮液中活细胞数目的方法。 该过程在短时间内提供量化数据，而不使用危险物质。 本发明的方法还可以用于定量带负电的粒子数，测定细胞毒性，细胞增殖测定和细胞分化测定。 此外，本发明提供了用于定量细胞或带负电粒子的测定试剂盒。

公开(公告)号：EP0787288B1

公开(公告)日：2009-03-04

申请号：EP95933559.7

申请日：1995-10-18

Applicant: Packard Instrument Company, Inc.

Inventor： ANSORGE, Richard, Eric ,  RUSHBROOKE, John, Gordon ,  HOOPER, Claire, Elizabeth ,  NEALE, William, Wray

IPC: G01N21/64 ,  C12M1/34

CPC classification number: G01N21/6456 ,  G01N2021/6441

Abstract: A method of detecting the presence and position of labelled material in a sample in which the labelled material either gives of light or can be stimulated to do so. The sample is imaged onto an image intensified CCD camera which is scanned following each exposure. Measurements are performed on the data signals so obtained, to identify clusters of data values from adjacent regions of the CCD array caused by light emitted by the image intensifier incident on those regions. The measured signal values are compared with at least one threshold so as to distinguish clusters resulting from light emitting regions of labelled material from the remainder of the sample and the centroid of each light produced cluster of data values is computed with reference to the camera array, and a signal value corresponding to the centroid coordinates is stored in a memory together with the centroid coordinates of any other light produced clusters identified during the same interrogation. The coordinates from each of a succession of interrogations of the same sample may be stored in an accumulation store to enable a list of the recorded light emissions and/or display of the events, to be produced by reading out the store. Apparatus for performing this method is also described.

公开(公告)号：EP1181099B1

公开(公告)日：2006-03-29

申请号：EP01942582.6

申请日：2001-01-11

Applicant: Packard Instrument Company, Inc.

Inventor： PAPEN, Roeland, E.

IPC: B01L3/02 ,  G01N1/10 ,  B01L3/00 ,  G01N35/00

CPC classification number: B01L3/0268 ,  B01J2219/00378 ,  B01J2219/00527 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/0063 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00691 ,  C40B60/14 ,  G01F1/48 ,  G01F3/00 ,  G01N35/1016 ,  G01N35/1065 ,  G01N2035/00237 ,  G01N2035/1018 ,  G01N2035/1025 ,  G01N2035/1034 ,  G01N2035/1039 ,  G01N2035/1041 ,  Y10T436/2575

Abstract: A system for aspirating and ejecting microvolume drops (26) of liquid onto porous sites of a substrate wafer includes a microdispenser (16) employing a piezoelectric transducer (60) attached to a glass capillary (62), a means for priming and aspirating transfer liquid (24) into the microdispenser (16), for controlling the pressure of the system liquid (20), and for washing the microdispenser (16) between liquid transfers, and a pressure sensor (14) to measure the system liquid pressure and produce a corresponding electrical signal. The drops are generally in the 10 to 100 micron range and the pores are generally 10 to 10,000 times smaller than the diameter of the drops deposited thereon. The resulting spots are uniform, and only slightly larger in diameter of the drops. The drops are ejected from a distance greater than the diameter of the drops, thus avoiding any contact with the dispenser that could damage the wafer. The system detects dispensing of a drop onto the reaction site.

公开(公告)号：EP1348120B1

公开(公告)日：2006-03-08

申请号：EP01272703.8

申请日：2001-12-18

Applicant: Packard Instrument Company, Inc.

Inventor： RUSHBROOKE, John, Gordon ,  HOOPER, Claire, Elizabeth

IPC: G01N21/64

CPC classification number: G01N21/6452 ,  G01N21/6456 ,  G01N21/76 ,  G01N2021/6484 ,  G01N2201/0826 ,  G01N2201/0833

Abstract: A fibre optic epi-fluorescence imaging system in which the optical fibres are rearranged so that the system can be used for measuring luminescence samples. The system comprises at least two optical fibres (32, 46) or bundles of fibres which lead to a CCD camera (74), the fibres or bundles of fibres from all samples being arranged in two sets, a first set which are formed from a non-fluorescing material and a second set which are formed from a material which may fluoresce but enables the fibres formed therefrom to have a higher numerical aperature than those of the first set.

公开(公告)号：EP1515135A3

公开(公告)日：2005-07-20

申请号：EP04028285.7

申请日：2000-04-14

Applicant: Packard Instrument Company, Inc.

Inventor： Rushbrooke, John Gordon ,  Hooper, Claire Elizabeth

IPC: G01N21/64 ,  G01N27/447

CPC classification number: G01N21/6452 ,  B01J2219/00317 ,  B01J2219/00707 ,  B01L3/5085 ,  B01L2300/0893 ,  C40B60/14 ,  G01N2021/6484 ,  G01N2201/0846

Abstract: A system for imaging radiation emitted by assay couples into a photoeletric detector, the system including a fibre optic bundle (100) for conveying light to the detector, wherein a microlens (118), preferably a drum leans, is located at the impact end of the fibre optic bundle to match the field of view of the bundle to a potential area of interest in a sample.