公开(公告)号：WO2020037121A1

公开(公告)日：2020-02-20

申请号：PCT/US2019/046649

申请日：2019-08-15

Applicant: HYPERFINE RESEARCH, INC. ,  4CATALYZER CORPORATION ,  LAZARUS, Carole ,  KUNDU, Prantik ,  TANG, Sunli ,  MOSHEN SALEHI, Seyed, Sadegh ,  SOFKA, Michal ,  SCHLEMPER, Jo ,  DYVORNE, Hadrien, A. ,  O'HALLORAN, Rafael ,  SACOLICK, Laura ,  POOLE, Michael, Stephen ,  ROTHBERG, Jonathan, M.

Inventor： LAZARUS, Carole ,  KUNDU, Prantik ,  TANG, Sunli ,  MOSHEN SALEHI, Seyed, Sadegh ,  SOFKA, Michal ,  SCHLEMPER, Jo ,  DYVORNE, Hadrien, A. ,  O'HALLORAN, Rafael ,  SACOLICK, Laura ,  POOLE, Michael, Stephen ,  ROTHBERG, Jonathan, M.

IPC: G06K9/40 ,  G01R33/56 ,  G06K9/46 ,  G06K9/62 ,  G06N3/04 ,  G06T5/00 ,  G01R33/34 ,  G01R33/385

Abstract: Techniques for removing artefacts, such as RF interference and/or noise, from magnetic resonance data. The techniques include: obtaining (302) input magnetic resonance data using at least one radio-frequency coil (526) of a magnetic resonance imaging system (500); and generating (306) a magnetic resonance image from the input magnetic resonance data at least in part by using a neural network model (130) to suppress (304, 308) at least one artefact in the input magnetic resonance data.

公开(公告)号：WO2010138182A3

公开(公告)日：2010-12-02

申请号：PCT/US2010/001543

申请日：2010-05-27

Applicant: LIFE TECHNOLOGIES CORPORATION ,  ROTHBERG, Jonathan M. ,  HINZ, Wolfgang ,  DAVIDSON, John F. ,  VAN OIJEN, Antoine M. ,  LEAMON, John H. ,  HUBER, Martin ,  BUSTILLO, James M. ,  MILGREW, Mark, James ,  SCHULTZ, Jonathan ,  MARRAN, David ,  REARICK, Todd ,  JOHNSON, Kim L.

Inventor： ROTHBERG, Jonathan M. ,  HINZ, Wolfgang ,  DAVIDSON, John F. ,  VAN OIJEN, Antoine M. ,  LEAMON, John H. ,  HUBER, Martin ,  BUSTILLO, James M. ,  MILGREW, Mark, James ,  SCHULTZ, Jonathan ,  MARRAN, David ,  REARICK, Todd ,  JOHNSON, Kim L.

IPC: A61M37/00 ,  A61B5/145

Abstract: Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by- synthesis reactions. Some methods provided herein relate to improving signal (and also signal-to-noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

公开(公告)号：WO2010008480A3

公开(公告)日：2010-06-03

申请号：PCT/US2009003766

申请日：2009-06-25

Applicant: ION TORRENT SYSTEMS INC ,  ROTHBERG JONATHAN M ,  HINZ WOLFGANG ,  JOHNSON KIM L ,  BUSTILLO JAMES M ,  LEAMON JOHN H ,  SCHULTZ JONATHAN

Inventor： ROTHBERG JONATHAN M ,  HINZ WOLFGANG ,  JOHNSON KIM L ,  BUSTILLO JAMES M ,  LEAMON JOHN H ,  SCHULTZ JONATHAN

IPC: C12Q1/68 ,  G01N27/414

CPC classification number: C12Q1/6874 ,  B01L3/502761 ,  B01L2300/046 ,  B01L2300/0663 ,  B01L2300/0851 ,  B01L2400/086 ,  C12Q1/6869 ,  G01N27/4145 ,  G01N27/4148 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2565/629

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

Abstract translation: 涉及用于分析物测量的超大规模FET阵列的方法和装置。 可以使用基于改善的FET像素和阵列设计的常规CMOS处理技术来制造ChemFET（例如，ISFET）阵列，这些技术增加了测量灵敏度和准确度，并且同时促进了显着小的像素尺寸和密集阵列。 改进的阵列控制技术可以从大型密集阵列中快速获取数据。 这种阵列可用于检测各种化学和/或生物过程中各种分析物类型的存在和/或浓度变化。 在一个实例中，chemFET阵列基于监测无机焦磷酸（PPi），氢离子和三磷酸核苷酸浓度的变化来促进DNA测序技术。

公开(公告)号：WO2007081385A8

公开(公告)日：2008-11-27

申请号：PCT/US2006021280

申请日：2006-06-01

Applicant: RAINDANCE TECHNOLOGIES INC ,  LINK DARREN R ,  BOITARD LAURENT ,  BRANCIFORTE JEFFREY ,  CHARLES YVES ,  FEKE GILBERT ,  LU JOHN Q ,  MARRAN DAVID ,  TABATABAI AHMADALI ,  WEINER MICHAEL ,  HINZ WOLFGANG ,  ROTHBERG JONATHAN M

Inventor： LINK DARREN R ,  BOITARD LAURENT ,  BRANCIFORTE JEFFREY ,  CHARLES YVES ,  FEKE GILBERT ,  LU JOHN Q ,  MARRAN DAVID ,  TABATABAI AHMADALI ,  WEINER MICHAEL ,  HINZ WOLFGANG ,  ROTHBERG JONATHAN M

IPC: G01N33/536 ,  B01J19/00 ,  C07B61/00 ,  C07K1/04 ,  C12Q1/68 ,  G01N33/68

CPC classification number: C12N15/1075 ,  B01F3/0807 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/00479 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00599 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/084 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/047 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2561/119 ,  C12Q2563/103 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/629 ,  C12Q2600/16 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/6458 ,  G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/6845 ,  G01N33/6854 ,  G01N2500/10

Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label, thereby identifying a single compound from a library of compounds.

Abstract translation: 本发明提供了可用于进行高通量筛选测定和组合化学的新型微流体装置和方法。 这样的方法可以包括通过将化合物的水溶液和独特的液体标签的水溶液乳化在微流体装置上来标记化合物文库，所述微流体装置包括多个可电寻址的通道支承的流体模块，其整体地布置在微细加工的基底上， 提供了用于不混溶流体流动的通道，由此每种化合物用独特的液体标记标记，汇集标记的乳液，将标记的乳液与含有特定细胞或酶的乳液聚结，从而形成纳米反应器，筛选纳米反应器以获得所需的反应 在纳米反应器的内容物之间，并解码液体标签，从而从化合物文库鉴定单一化合物。

公开(公告)号：WO2008063227A3

公开(公告)日：2008-11-06

申请号：PCT/US2007011460

申请日：2007-05-11

Applicant: RAINDANCE TECHNOLOGIES INC ,  LINK DARREN R ,  WEINER MICHAEL ,  MARREN DAVID ,  ROTHBERG JONATHAN M

Inventor： LINK DARREN R ,  WEINER MICHAEL ,  MARREN DAVID ,  ROTHBERG JONATHAN M

IPC: B01F3/08 ,  B01F13/00 ,  B01J19/00 ,  B01L3/00 ,  B81C1/00 ,  G01N15/14 ,  G01N33/50

CPC classification number: B01F3/0807 ,  B01F13/0062 ,  B01J19/0093 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502784 ,  B01L3/565 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  C12N15/1068 ,  C12N15/1086 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2565/628 ,  C12Q2565/629 ,  G01N15/147 ,  G01N21/05 ,  G01N21/64 ,  G01N27/3275 ,  G01N35/08 ,  G01N2021/0346 ,  G01N2035/00237 ,  G01N2035/00326 ,  G01N2201/024 ,  Y10T137/87571 ,  Y10T137/87619 ,  Y10T137/87652 ,  C12Q2525/191 ,  C12Q2563/159 ,  C12Q2535/101 ,  C12Q2563/149

Abstract: The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.

Abstract translation: 本发明提供了可用于进行生物学，化学和诊断测定的新型微流体底物和方法。 基板可以包括多个可电寻址的通道支承流体模块，其一体地布置成使得提供用于不混溶流体流动的连续通道。

公开(公告)号：WO2008076406A8

公开(公告)日：2008-09-12

申请号：PCT/US2007025721

申请日：2007-12-14

Applicant: ION TORRENT SYSTEMS INC ,  ROTHBERG JONATHAN M ,  HINZ WOLFGANG ,  JOHNSON KIM L ,  BUSTILLO JAMES

Inventor： ROTHBERG JONATHAN M ,  HINZ WOLFGANG ,  JOHNSON KIM L ,  BUSTILLO JAMES

IPC: G01N27/414 ,  G01N33/543

CPC classification number: G01N27/4148 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N33/54373 ,  G01N33/5438 ,  H01L27/088 ,  H01L29/42324 ,  H01L29/78 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01073 ,  H01L2924/14 ,  H01L2924/1433 ,  Y10T436/22 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101

Abstract: A method for sequencing a nucleic acid may include disposing a plurality of template nucleic acids into a plurality of reaction chambers, wherein the plurality of reaction chambers is in contact with a chemical-sensitive field effect transistor (chemFET) array comprising at least one chemFET for each reaction chamber, wherein the at least one chemFET has a floating gate, and wherein each of the template nucleic acids is hybridized to a sequencing primer and is bound to a polymerase. The method may further include synthesizing a new nucleic acid strand by incorporating one or more known nucleotide triphosphates sequentially at the 3' end of the sequencing primer and detecting the incorporation of the one or more known nucleotide triphosphates by a change in current at the at least one chemFET within the array.

公开(公告)号：WO2007081385A3

公开(公告)日：2007-07-19

申请号：PCT/US2006/021280

申请日：2006-06-01

Applicant: RAINDANCE TECHNOLOGIES, INC. ,  LINK, Darren, R. ,  BOITARD, Laurent ,  BRANCIFORTE, Jeffrey ,  CHARLES, Yves ,  FEKE, Gilbert ,  LU, John, Q. ,  MARRAN, David ,  TABATABAI, Ahmadali ,  WEINER, Michael ,  HINZ, Wolfgang ,  ROTHBERG, Jonathan, M.

Inventor： LINK, Darren, R. ,  BOITARD, Laurent ,  BRANCIFORTE, Jeffrey ,  CHARLES, Yves ,  FEKE, Gilbert ,  LU, John, Q. ,  MARRAN, David ,  TABATABAI, Ahmadali ,  WEINER, Michael ,  HINZ, Wolfgang ,  ROTHBERG, Jonathan, M.

IPC: G01N33/536 ,  C12Q1/68 ,  B01J19/00 ,  C07B61/00 ,  C07K1/04 ,  G01N33/68

Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label, thereby identifying a single compound from a library of compounds.

公开(公告)号：WO2005049652A3

公开(公告)日：2006-01-12

申请号：PCT/US2004038488

申请日：2004-11-17

Applicant: ROTHBERG INST FOR CHILDHOOD DI ,  WEINER MICHAEL P ,  ROTHBERG JONATHAN M

Inventor： WEINER MICHAEL P ,  ROTHBERG JONATHAN M

IPC: C07K16/00 ,  C07K16/24 ,  C07K16/28 ,  C12N15/10 ,  C12Q1/68

CPC classification number: C07K16/2803 ,  C07K16/241 ,  C07K2317/34 ,  C07K2317/622

Abstract: The present invention provides a high throughput method for generating fully human monoclonal antibodies.

Abstract translation: 本发明提供了用于产生完全人单克隆抗体的高通量方法。

公开(公告)号：WO2010008480A2

公开(公告)日：2010-01-21

申请号：PCT/US2009/003766

申请日：2009-06-25

Applicant: ION TORRENT SYSTEMS INCORPORATED ,  ROTHBERG, Jonathan, M. ,  HINZ, Wolfgang ,  JOHNSON, Kim, L. ,  BUSTILLO, James, M. ,  LEAMON, John, H. ,  SCHULTZ, Jonathan

Inventor： ROTHBERG, Jonathan, M. ,  HINZ, Wolfgang ,  JOHNSON, Kim, L. ,  BUSTILLO, James, M. ,  LEAMON, John, H. ,  SCHULTZ, Jonathan

IPC: C12Q1/68 ,  G01N27/414

CPC classification number: C12Q1/6874 ,  B01L3/502761 ,  B01L2300/046 ,  B01L2300/0663 ,  B01L2300/0851 ,  B01L2400/086 ,  C12Q1/6869 ,  G01N27/4145 ,  G01N27/4148 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2565/629

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

Abstract translation: 与用于分析物测量的非常大规模的FET阵列相关的方法和装置。 可以使用基于提高测量灵敏度和精度的改进的FET像素和阵列设计的传统CMOS处理技术来制造ChemFET（例如，ISFET）阵列，并且同时促进显着小的像素尺寸和致密阵列。 改进的阵列控制技术提供了从大型和密集阵列的快速数据采集。 可以使用这样的阵列来检测各种化学和/或生物过程中各种分析物类型的存在和/或浓度变化。 在一个示例中，chemFET阵列基于监测无机焦磷酸盐（PPi），氢离子和核苷酸三磷酸盐的浓度变化来促进DNA测序技术。

公开(公告)号：WO2007081387A1

公开(公告)日：2007-07-19

申请号：PCT/US2006/021380

申请日：2006-06-01

Applicant: RAINDANCE TECHNOLOGIES, INC. ,  LINK, Darren, R. ,  BOITARD, Laurent ,  BRANCIFORTE, Jeffrey ,  CHARLES, Yves ,  FEKE, Gilbert ,  LU, John, Q. ,  MARRAN, David ,  TABATABAI, Ahmadali ,  WEINER, Michael ,  HINZ, Wolfgang ,  ROTHBERG, Jonathan, M.

Inventor： LINK, Darren, R. ,  BOITARD, Laurent ,  BRANCIFORTE, Jeffrey ,  CHARLES, Yves ,  FEKE, Gilbert ,  LU, John, Q. ,  MARRAN, David ,  TABATABAI, Ahmadali ,  WEINER, Michael ,  HINZ, Wolfgang ,  ROTHBERG, Jonathan, M.

IPC: G01N33/536 ,  C12Q1/68 ,  B01J19/00 ,  C07B61/00 ,  C07K1/04 ,  G01N33/68

CPC classification number: C12N15/1075 ,  B01F3/0807 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/00479 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00599 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/084 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/047 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2561/119 ,  C12Q2563/103 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/629 ,  C12Q2600/16 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/6458 ,  G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/6845 ,  G01N33/6854 ,  G01N2500/10

Abstract: The present invention provides novel microfluidic devices, kits, and methods that are useful for performing high-throughput screening assays and diagnostics. Such diagnostic methods can include emulsifying an aqueous library of compounds with a set of uniquely dyecoded-labeled droplets in an inert fluorocarbon medium, thereby forming an interactor library, emulsifying an aqueous sample from a subject in an inert fluorocarbon medium, wherein said sample contains a compound that will react with at least one interactor molecule from the interactor library, coalescing the emulsions to form a nanoreactor, and screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, herein one or more steps are performed on a microfluidic device, which can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids.

Abstract translation: 本发明提供了用于进行高通量筛选测定和诊断的新型微流体装置，试剂盒和方法。 这样的诊断方法可以包括用惰性碳氟介质中的一组独特的经编码标记的液滴乳化化合物的水性文库，从而形成相互作用文库，在惰性碳氟介质中乳化来自受试者的含水样品，其中所述样品含有 将与来自相互作用文库的至少一个相互作用分子反应的化合物，聚合乳液以形成纳米反应器，并筛选纳米反应器以获得纳米反应器内容物之间所需的反应，这里，一个或多个步骤在微流体装置上进行， 其可以包括整体地布置在微加工基板上的多个可电寻址的通道支承流体模块，从而为不混溶流体的流动提供连续通道。