-
公开(公告)号：KR100815439B1
公开(公告)日：2008-03-20
申请号：KR1020070014897
申请日：2007-02-13
Applicant: 강원대학교산학협력단
CPC classification number: A01H4/005 , A01H4/001 , A01H4/008 , C12N5/0025 , C12N2500/34 , C12N2501/30
Abstract: A method for mass-producing an Oplopanax elatus Nakai plantlet is provided to mass-propagate the Oplopanax elatus Nakai effectively from embryogenetic callus derived from an Oplopanax elatus Nakai tissue, thereby preserving endangered Oplopanax elatus Nakai. A method for mass-producing an Oplopanax elatus Nakai plantlet comprises the steps of: (a) explanting a tissue segment of Oplopanax elatus Nakai a cell tissue cultures in an MS culture medium where a plant growth control material including TDZ is added to induce embryogenetic callus; (b) culturing the induced embryogenetic callus in a liquid culture medium or a solid culture medium to induce somatic embryos; (c) culturing the induced somatic embryos in a liquid culture medium or a solid culture medium to propagate them; (d) culturing the propagated somatic embryos in a liquid culture medium or a solid culture medium having a pH of 5.0-5.75 to redifferentiate a plantlet; and (e) acclimatizing the redifferentiated plantlet in a soil where bed soil and sand are mixed in a ratio of 1:1, wherein the culture medium of the step(a) is an MS culture medium where 2,4-D or NAA is further added as the plant growth controlling material. Further, the MS culture medium comprises 10-30 mg/l of sucrose as carbon source.
Abstract translation: 提供了一种大规模生产Oplopanax elatus Nakai小植物的方法，有效地从Oplopanax elatus Nakai组织的胚胎发生愈伤组织中大量繁殖Oplopanax elatus Nakai，从而保存濒危的Oplopanax elatus Nakai。 一种大规模生产Oplopanax elatus Nakai小植物的方法包括以下步骤：（a）在MS培养基中移除Oplopanax elatus Nakai的组织段，其中加入包含TDZ的植物生长控制材料以诱导胚胎发生愈伤组织 ; （b）在液体培养基或固体培养基中培养诱导的胚胎发生愈伤组织以诱导体细胞胚; （c）在液体培养基或固体培养基中培养诱导的体细胞胚以繁殖它们; （d）在液体培养基或pH5.0-5.75的固体培养基中培养繁殖的体细胞胚以再分化植物; 和（e）将所述再分化的小植物驯化在比例为1:1的床土壤和沙子的土壤中，其中步骤（a）的培养基是MS培养基，其中2,4-D或NAA为 进一步添加作为植物生长控制材料。 此外，MS培养基包含10-30mg / l蔗糖作为碳源。
-
公开(公告)号：KR101857005B1
公开(公告)日：2018-05-14
申请号：KR1020160048072
申请日：2016-04-20
Applicant: 강원대학교산학협력단
Abstract: 본발명은게르마늄을함유하는땃두릅나무부정근의생산방법, 상기방법에의해생산된게르마늄을함유하는땃두릅나무부정근및 상기게르마늄을함유하는땃두릅나무부정근을유효성분으로하는항산화및 피부미백용화장료조성물에관한것이다. 본발명은땃두릅나무부정근배양과정에서, 유기산및 킬레이트를처리하는방법과 2단계배양법을통하여배양하였을때 부정근내 유기게르마늄축적및 생산이효과적으로이루어지며, 상기유기게르마늄을축적한땃두릅나무부정근은피부미백, 항산화활성및 총페놀함량이증가하는효과를나타냄을확인하였다. 본발명의방법에의해제조된부정근을이용하여땃두릅나무의유용성분뿐만아니라유기게르마늄생산에의한고기능성원료를확보하고, 이의피부미백및 항산화증진효과를확인하여산업화에부합되는소재로서의개발을가능하게할 것으로기대된다.
-
公开(公告)号：KR101653770B1
公开(公告)日：2016-09-02
申请号：KR1020140124186
申请日：2014-09-18
Applicant: 강원대학교산학협력단
Abstract: 본발명은땃두릅나무부정근의대량생산방법및 상기방법에의해생산된땃두릅나무부정근에관한것이다. 본발명의땃두릅나무부정근의생산방법으로부터부정근을유도, 증식한후 이로부터최적의기내배양조건을규명함으로써대량생산체계의기초를확립하였다. 본발명의방법에의해제조된부정근을이용하여땃두릅나무의유용성분을생산하고, 멸종위기의땃두릅나무종묘생산에도활용이가능하므로그 이용가치가높을것이다.
-
Patent Agency Ranking
公开(公告)号：KR101537806B1
公开(公告)日：2015-07-29
申请号：KR1020130098930
申请日：2013-08-21
Applicant: 강원대학교산학협력단
Abstract: 본발명은옻나무추출물을유효성분으로함유하는식물병원균방제및 내성증진용조성물에관한것으로, 더욱상세하게는옻나무추출물을유효성분으로함유하는식물병원균방제및 내성증진용조성물, 상기조성물을식물에처리하는단계를포함하는식물의병원균방제또는내성증진방법및 옻나무추출물을유효성분으로함유하는식물병원균방제및 내성증진용생물농약에관한것으로, 본발명의옻나무추출물은알러지를유발하는독성물질인우루시올(urushiol)이제거되어소비자에게안전하게제공될수 있을뿐만아니라, 식물의초기생육을촉진시켜주고, 재배시문제가되는식물병원균방어관련유전자들이강하게발현되고, 실질적으로바이러스병에대한저항성을나타내므로본 발명의옻나무추출물은사용자에게는안전하면서재배작물에게는유용한식물병원균방제또는내성증진용생물농약으로유용하게사용될수 있다.
-
公开(公告)号：KR1020160033408A
公开(公告)日：2016-03-28
申请号：KR1020140124186
申请日：2014-09-18
Applicant: 강원대학교산학협력단
CPC classification number: A01H4/001 , A01H4/00 , A01H4/005 , C12N5/0025 , C12N2500/34 , C12N2501/30 , C12N2529/10
Abstract: 본발명은땃두릅나무부정근의대량생산방법및 상기방법에의해생산된땃두릅나무부정근에관한것이다. 본발명의땃두릅나무부정근의생산방법으로부터부정근을유도, 증식한후 이로부터최적의기내배양조건을규명함으로써대량생산체계의기초를확립하였다. 본발명의방법에의해제조된부정근을이용하여땃두릅나무의유용성분을생산하고, 멸종위기의땃두릅나무종묘생산에도활용이가능하므로그 이용가치가높을것이다.
Abstract translation: 本发明涉及一种用于大量生产卵黄的不定根的方法以及由此产生的黄鳝的不定根。 本发明通过使用大规模生产Oplopanax elatus的不定根的方法诱导不定根，扩散大量生产系统，从而产生最佳的体外培养条件，从而建立了大规模生产系统的基础。 本发明可以非常有用，因为通过本发明的方法生产的Oplopanax elatus的不定根可以用于生产Oplopanax elatus的活性成分和生产濒危的Oplopanax种的幼苗。
-
公开(公告)号：KR1020140104400A
公开(公告)日：2014-08-28
申请号：KR1020140095608
申请日：2014-07-28
Applicant: 강원대학교산학협력단
CPC classification number: Y02P60/149
Abstract: The present invention relates to a method for increasing biomass of ginseng adventitious roots, including a step of cultivating ginseng adventitious roots by irradiating the ginseng adventitious roots with red light with a wavelength of 625 to 630 nm from a light emitting diode (LED). Using the irradiation of a specific wavelength by the LED according to the present invention, provided is a method for mass production of ginseng adventitious roots, which achieves the increase of biomass as well as the development and growth of favorable ginseng adventitious roots by developing a most proliferous condition for cultivating ginseng adventitious roots in bioreactors, and thus industrializes the mass production of ginseng adventitious roots in bioreactors. Accordingly, high value-added ginseng can be produced all the year around without being affected by the conditions of climate or surroundings and offered to various consumer groups at affordable prices.
Abstract translation: 本发明涉及一种提高人参不定根生物量的方法，其中包括通过用发光二极管（LED）从波长为625至630nm的红光照射人参不定根而培养人参不定根的步骤。 使用根据本发明的LED照射特定波长的方法，提供了人参不定根大规模生产的方法，其通过开发最多的方法实现生物量的增加以及有利人参不定根的发育和生长 在生物反应器中培养人参不定根的繁殖条件，从而将生物反应器中人参不定根的大量生产工业化。 因此，高附加值人参可以全年生产，不受气候或环境条件的影响，并以合理的价格向各消费群体提供。
-
17.发明授权
公开(公告)号：KR100815436B1
公开(公告)日：2008-03-20
申请号：KR1020070014884
申请日：2007-02-13
Applicant: 강원대학교산학협력단
CPC classification number: A01H4/005 , A01H4/001 , A01H4/008 , C12N5/0025 , C12N2500/34
Abstract: A method for mass-producing an Oplopanax elatus Nakai plantlet is provided to establish an in vitro mass-production system of the Oplopanax elatus Nakai, thereby mass-propagating the Oplopanax elatus Nakai with useful medicinal effects but hard to be bred. A method for mass-producing an Oplopanax elatus Nakai plantlet comprises the steps of: (a) explanting a tissue segment of Oplopanax elatus Nakai roots in an MS culture medium to induce directly somatic embryos; (b) culturing the induced somatic embryos in a liquid culture medium or a solid culture medium to propagate them; (c) culturing the propagated somatic embryos in a liquid culture medium or a solid culture medium having a pH of 5.0-5.75 to redifferentiate a plantlet; and (d) acclimatizing the redifferentiated plantlet in a soil where bed soil and sand are mixed in a ratio of 1:1.
Abstract translation: 提供了一种大规模生产Oplopanax elatus Nakai小植株的方法，建立了Oplopanax elatus Nakai的体外批量生产系统，从而大量繁殖了具有有用药用效果但难以繁殖的Oplopanax elatus Nakai。 一种大规模生产Oplopanax elatus Nakai小植株的方法包括以下步骤：（a）在MS培养基中除去Oplopanax elatus Nakai根的组织段，诱导直接体细胞胚; （b）在液体培养基或固体培养基中培养诱导的体细胞胚以繁殖它们; （c）在液体培养基或pH5.0-5.75的固体培养基中培养繁殖的体细胞胚以再分化植物; 和（d）将土壤和沙子以1:1的比例混合的土壤中的再分化苗进行驯化。
-
公开(公告)号：KR100788290B1
公开(公告)日：2007-12-27
申请号：KR1020070014919
申请日：2007-02-13
Applicant: 강원대학교산학협력단
CPC classification number: A01H4/005 , A01H4/001 , A01H4/008 , C12N5/0025 , C12N2500/34 , C12N2501/30
Abstract: A method for mass-propagating Oplopanax elatus Nakai plantlet is provided to establish the in vitro mass-production system of Oplopanax elatus Nakai, one of endangered plant species, thereby mass-producing the Oplopanax elatus Nakai which has useful pharmacological effects and is hard to be propagated. A method for mass-propagating Oplopanax elatus Nakai plantlet comprises the steps of: (a) germinating washed Oplopanax elatus Nakai seeds in an SH medium or a B5 medium at a temperature of 25 deg.C for 16 hours light condition and 8 hours dark condition; (b) culturing a fragment body of the germinated in vitro plant in a liquid medium or a solid medium to induce somatic embryos; (c) culturing the induced somatic embryos in a liquid medium or a solid medium to proliferate them; (d) culturing the proliferated somatic embryos in a liquid medium or a solid medium to redifferentiate a plantlet; and (e) acclimatizing the redifferentiated platelet in soil consisting of bed soil and sand in a mixture ratio of 1:1.
Abstract translation: 提供了一种大规模生产Oplopanax elatus Nakai小植物的方法，建立了濒危植物种类的Oplopanax elatus Nakai的体外批量生产系统，从而大量生产具有有效药理作用的Oplopanax elatus Nakai，难以成为 传播。 大量繁殖Oplopanax elatus Nakai小植物的方法包括以下步骤：（a）在SH培养基或B5培养基中在25℃的温度下将洗涤的Oplopanax elatus Nakai种子发芽16小时光条件和8小时黑暗条件 ; （b）在液体培养基或固体培养基中培养发芽的体外植物的片段体以诱导体细胞胚; （c）在液体培养基或固体培养基中培养诱导的体细胞胚以使其增殖; （d）在液体培养基或固体培养基中培养增殖的体细胞胚以再分化植物; 和（e）以1：1的混合比适应由床土和沙子组成的土壤中的再分化血小板。
-
-
-
-
-
-
-
Search Results
18
records
(took 0.018 seconds)
