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公开(公告)号：KR1020120056733A
公开(公告)日：2012-06-04
申请号：KR1020100118410
申请日：2010-11-25
Applicant: 주식회사 세바바이오텍 , 강원대학교산학협력단
CPC classification number: A61K8/97 , A61K8/99 , A61K36/258 , A61K36/48 , A61K36/81 , A61K2236/19 , A61K2236/33 , A61Q19/00 , Y10S435/839 , Y10S514/844
Abstract: PURPOSE: A cosmetic composition containing composite plant fermentation extract is provided to ensure high antioxidation efficiency and to enhance skin absorption and physiological efficiency. CONSTITUTION: A method for preparing composite plant fermentation extract for cosmetics comprises: a step of putting Bacillus subtilis to a mixture of steamed soybean and tomato powder to prepare a fermentation; and a step of obtaining hot water extract or organic solvent extract from the fermentation. The fermentation further contains fermented ginseng powder. A cosmetic composition contains the composite plant fermentation extract.
Abstract translation: 目的：提供含有复合植物发酵提取物的化妆品组合物，以确保高抗氧化效果，增强皮肤吸收和生理效能。 构成：用于制备化妆品复合植物发酵提取物的方法包括：将枯草芽孢杆菌置于蒸大豆和番茄粉混合物中以制备发酵的步骤; 以及从发酵中获得热水提取物或有机溶剂提取物的步骤。 发酵还含有发酵人参粉末。 化妆品组合物含有复合植物发酵提取物。
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Patent Agency Ranking
公开(公告)号：KR100815439B1
公开(公告)日：2008-03-20
申请号：KR1020070014897
申请日：2007-02-13
Applicant: 강원대학교산학협력단
CPC classification number: A01H4/005 , A01H4/001 , A01H4/008 , C12N5/0025 , C12N2500/34 , C12N2501/30
Abstract: A method for mass-producing an Oplopanax elatus Nakai plantlet is provided to mass-propagate the Oplopanax elatus Nakai effectively from embryogenetic callus derived from an Oplopanax elatus Nakai tissue, thereby preserving endangered Oplopanax elatus Nakai. A method for mass-producing an Oplopanax elatus Nakai plantlet comprises the steps of: (a) explanting a tissue segment of Oplopanax elatus Nakai a cell tissue cultures in an MS culture medium where a plant growth control material including TDZ is added to induce embryogenetic callus; (b) culturing the induced embryogenetic callus in a liquid culture medium or a solid culture medium to induce somatic embryos; (c) culturing the induced somatic embryos in a liquid culture medium or a solid culture medium to propagate them; (d) culturing the propagated somatic embryos in a liquid culture medium or a solid culture medium having a pH of 5.0-5.75 to redifferentiate a plantlet; and (e) acclimatizing the redifferentiated plantlet in a soil where bed soil and sand are mixed in a ratio of 1:1, wherein the culture medium of the step(a) is an MS culture medium where 2,4-D or NAA is further added as the plant growth controlling material. Further, the MS culture medium comprises 10-30 mg/l of sucrose as carbon source.
Abstract translation: 提供了一种大规模生产Oplopanax elatus Nakai小植物的方法，有效地从Oplopanax elatus Nakai组织的胚胎发生愈伤组织中大量繁殖Oplopanax elatus Nakai，从而保存濒危的Oplopanax elatus Nakai。 一种大规模生产Oplopanax elatus Nakai小植物的方法包括以下步骤：（a）在MS培养基中移除Oplopanax elatus Nakai的组织段，其中加入包含TDZ的植物生长控制材料以诱导胚胎发生愈伤组织 ; （b）在液体培养基或固体培养基中培养诱导的胚胎发生愈伤组织以诱导体细胞胚; （c）在液体培养基或固体培养基中培养诱导的体细胞胚以繁殖它们; （d）在液体培养基或pH5.0-5.75的固体培养基中培养繁殖的体细胞胚以再分化植物; 和（e）将所述再分化的小植物驯化在比例为1:1的床土壤和沙子的土壤中，其中步骤（a）的培养基是MS培养基，其中2,4-D或NAA为 进一步添加作为植物生长控制材料。 此外，MS培养基包含10-30mg / l蔗糖作为碳源。
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公开(公告)号：KR1020100122543A
公开(公告)日：2010-11-23
申请号：KR1020090041487
申请日：2009-05-13
Applicant: 강원대학교산학협력단
IPC: A61K36/899 , A61K8/97 , A61Q19/00 , A61P31/04
CPC classification number: Y02A50/473 , Y02A50/481
Abstract: PURPOSE: A Sorghum bicolor Moench extract having an antibacterial activity is provided to prevent side effects by human body toxicity accumulation and to use as an alternative agent of conventional synthetic antibacterial agent. CONSTITUTION: A Sorghum bicolor Moench extract is isolated using low alcohol, methanol. The Sorghum bicolor Moench extract is fractioned using hexane, ethyl acetate, n-butanol or water. A cosmetic composition for anti-bacteria contains the Sorghum bicolor Moench extract as an active ingredient. The composition has antibacterial activities to Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, or Klebsiella pneumoniae. The cosmetic composition is used in the form of skin softener, nutrition skin, nutrition cream, essence, pack or shower powder.
Abstract translation: 目的：提供具有抗菌活性的高粱双色Moench提取物，以防止人体毒性积累的副作用，并用作常规合成抗菌剂的替代试剂。 构成：使用低酒精，甲醇分离高粱双色Moench提取物。 高粱双色Moench提取物用己烷，乙酸乙酯，正丁醇或水分级。 抗菌化妆品组合物含有高粱双色Moench提取物作为活性成分。 该组合物对金黄色葡萄球菌，大肠杆菌，鼠伤寒沙门氏菌或肺炎克雷伯杆菌具有抗菌活性。 化妆品组合物以皮肤柔软剂，营养皮肤，营养霜，精华液，包装或淋浴粉的形式使用。
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公开(公告)号：KR101315277B1
公开(公告)日：2013-10-07
申请号：KR1020100118410
申请日：2010-11-25
Applicant: 주식회사 세바바이오텍 , 강원대학교산학협력단
Abstract: 본 발명은 (a) 증자한 대두 및 토마토분말의 혼합물에 고초균을 투입하여 발효물을 제조하는 단계; 및
(b) 상기 단계 (a)에서 얻은 발효물로부터 열수 또는 유기용매의 추출물을 얻는 단계를 포함하는 화장료용 복합식물발효 추출물의 제조방법 및 동 복합식물발효 추출물을 함유하는 화장료 조성물 제공한다.-
公开(公告)号：KR100815436B1
公开(公告)日：2008-03-20
申请号：KR1020070014884
申请日：2007-02-13
Applicant: 강원대학교산학협력단
CPC classification number: A01H4/005 , A01H4/001 , A01H4/008 , C12N5/0025 , C12N2500/34
Abstract: A method for mass-producing an Oplopanax elatus Nakai plantlet is provided to establish an in vitro mass-production system of the Oplopanax elatus Nakai, thereby mass-propagating the Oplopanax elatus Nakai with useful medicinal effects but hard to be bred. A method for mass-producing an Oplopanax elatus Nakai plantlet comprises the steps of: (a) explanting a tissue segment of Oplopanax elatus Nakai roots in an MS culture medium to induce directly somatic embryos; (b) culturing the induced somatic embryos in a liquid culture medium or a solid culture medium to propagate them; (c) culturing the propagated somatic embryos in a liquid culture medium or a solid culture medium having a pH of 5.0-5.75 to redifferentiate a plantlet; and (d) acclimatizing the redifferentiated plantlet in a soil where bed soil and sand are mixed in a ratio of 1:1.
Abstract translation: 提供了一种大规模生产Oplopanax elatus Nakai小植株的方法，建立了Oplopanax elatus Nakai的体外批量生产系统，从而大量繁殖了具有有用药用效果但难以繁殖的Oplopanax elatus Nakai。 一种大规模生产Oplopanax elatus Nakai小植株的方法包括以下步骤：（a）在MS培养基中除去Oplopanax elatus Nakai根的组织段，诱导直接体细胞胚; （b）在液体培养基或固体培养基中培养诱导的体细胞胚以繁殖它们; （c）在液体培养基或pH5.0-5.75的固体培养基中培养繁殖的体细胞胚以再分化植物; 和（d）将土壤和沙子以1:1的比例混合的土壤中的再分化苗进行驯化。
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公开(公告)号：KR100788290B1
公开(公告)日：2007-12-27
申请号：KR1020070014919
申请日：2007-02-13
Applicant: 강원대학교산학협력단
CPC classification number: A01H4/005 , A01H4/001 , A01H4/008 , C12N5/0025 , C12N2500/34 , C12N2501/30
Abstract: A method for mass-propagating Oplopanax elatus Nakai plantlet is provided to establish the in vitro mass-production system of Oplopanax elatus Nakai, one of endangered plant species, thereby mass-producing the Oplopanax elatus Nakai which has useful pharmacological effects and is hard to be propagated. A method for mass-propagating Oplopanax elatus Nakai plantlet comprises the steps of: (a) germinating washed Oplopanax elatus Nakai seeds in an SH medium or a B5 medium at a temperature of 25 deg.C for 16 hours light condition and 8 hours dark condition; (b) culturing a fragment body of the germinated in vitro plant in a liquid medium or a solid medium to induce somatic embryos; (c) culturing the induced somatic embryos in a liquid medium or a solid medium to proliferate them; (d) culturing the proliferated somatic embryos in a liquid medium or a solid medium to redifferentiate a plantlet; and (e) acclimatizing the redifferentiated platelet in soil consisting of bed soil and sand in a mixture ratio of 1:1.
Abstract translation: 提供了一种大规模生产Oplopanax elatus Nakai小植物的方法，建立了濒危植物种类的Oplopanax elatus Nakai的体外批量生产系统，从而大量生产具有有效药理作用的Oplopanax elatus Nakai，难以成为 传播。 大量繁殖Oplopanax elatus Nakai小植物的方法包括以下步骤：（a）在SH培养基或B5培养基中在25℃的温度下将洗涤的Oplopanax elatus Nakai种子发芽16小时光条件和8小时黑暗条件 ; （b）在液体培养基或固体培养基中培养发芽的体外植物的片段体以诱导体细胞胚; （c）在液体培养基或固体培养基中培养诱导的体细胞胚以使其增殖; （d）在液体培养基或固体培养基中培养增殖的体细胞胚以再分化植物; 和（e）以1：1的混合比适应由床土和沙子组成的土壤中的再分化血小板。
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