rhynchophylla Hance) 의 종자 내 배 유래 체세포배를 이용하여 생물반응기를 이용하여 유식물체를 대량생산하는 방법에 관한 것으로, 더욱 구체적으로 물푸레나무 종자의 날개와 배유를 제거하고 자엽 부분을 포함하는 배의 끝부분을 절단하는 단계; 절단된 배를 2,4-D를 포함하는 1/3 MS 배지에서 암배양을 수행한 후 다시 2,4-D가 첨가되지 않은 1/3로 줄인 MS 배지로 옮겨 광 조건 하에서 배발생 캘러스를 유도하는 단계; 유도된 캘러스를 15~17시간의 광주기로 3~5주간 조사하면서 배양하여 체세포배를 유도하는 단계; 유도된 체세포배를 공기부양식 생물반응기에 접종하고, 8~12vvm 공기를 주입하고, 1/2 MS 배지를 사용하여 25~35일 배양하여 체세포배를 증식하는 단계; 증식된 체세포배를 1/3 MS 배지의 고체 배지에 이식하여 유식물체를 형성하는 단계; 및 상기 유식물체를 식물체로 순화시키는 단계를 포함하는 생물반응기를 이용한 물푸레나무의 대량 증식 방법에 관한 것이다.
Abstract:
PURPOSE: A cosmetic composition containing composite plant fermentation extract is provided to ensure high antioxidation efficiency and to enhance skin absorption and physiological efficiency. CONSTITUTION: A method for preparing composite plant fermentation extract for cosmetics comprises: a step of putting Bacillus subtilis to a mixture of steamed soybean and tomato powder to prepare a fermentation; and a step of obtaining hot water extract or organic solvent extract from the fermentation. The fermentation further contains fermented ginseng powder. A cosmetic composition contains the composite plant fermentation extract.
Abstract:
PURPOSE: A composition for anti-obesity containing Liriope platyphylla Wang et Tang seed extract is provided to suppress alpha-glucosidase, alpha-amylase, and lipase and to prevent and treat obesity. CONSTITUTION: A composition for anti-obesity contains Liriope platyphylla Wang et Tang seed extract as an active ingredient. The composition contains anthocyanine-based colorant of chemical formula 1 isolated from the extract as an active ingredient. The extract is prepared using water, low alcohol of 1-4 carbon atoms, or mixture thereof. A health food for anti-obesity contains the extract as an active ingredient.
Abstract translation:目的:提供含有Liriope platyphylla Wang et Tang种子提取物的抗肥胖组合物,用于抑制α-葡萄糖苷酶,α-淀粉酶和脂肪酶,并预防和治疗肥胖。 构成:抗肥胖组合物含有Liriope platyphylla Wang et Tang种子提取物作为活性成分。 该组合物含有从提取物中分离出的作为活性成分的化学式1的基于花青的着色剂。 提取物是使用水,1-4个碳原子的低级醇或其混合物制备的。 用于抗肥胖的保健食品含有提取物作为活性成分。
Abstract:
A method for mass-producing an Oplopanax elatus Nakai plantlet is provided to mass-propagate the Oplopanax elatus Nakai effectively from embryogenetic callus derived from an Oplopanax elatus Nakai tissue, thereby preserving endangered Oplopanax elatus Nakai. A method for mass-producing an Oplopanax elatus Nakai plantlet comprises the steps of: (a) explanting a tissue segment of Oplopanax elatus Nakai a cell tissue cultures in an MS culture medium where a plant growth control material including TDZ is added to induce embryogenetic callus; (b) culturing the induced embryogenetic callus in a liquid culture medium or a solid culture medium to induce somatic embryos; (c) culturing the induced somatic embryos in a liquid culture medium or a solid culture medium to propagate them; (d) culturing the propagated somatic embryos in a liquid culture medium or a solid culture medium having a pH of 5.0-5.75 to redifferentiate a plantlet; and (e) acclimatizing the redifferentiated plantlet in a soil where bed soil and sand are mixed in a ratio of 1:1, wherein the culture medium of the step(a) is an MS culture medium where 2,4-D or NAA is further added as the plant growth controlling material. Further, the MS culture medium comprises 10-30 mg/l of sucrose as carbon source.
Abstract:
본 발명은 녹차로부터 클로로필류를 추출하는 방법에 관한 것으로서, 더욱 상세하게는 녹차로부터 분리된 클로로필류의 생리활성적 고찰과 이러한 색소계 화합물의 사용에 대한 특성을 파악하기 위하여 녹차로부터 클로로필류를 정제된 형태로 분리시키고, 분리된 각 색소 화합물에 대한 항산화 활성 및 항암활성을 검정하여 이들이 항산화 및 항암 활성을 가짐을 확인한 발명에 관한 것이다. 본 발명에 의하면, 녹차의 새로운 생리활성 기능을 밝히면서 항암 조성물의 새로운 원천을 제시할 수 있는 가능성 및 녹차의 항암 조성물로서의 새로운 기능을 제시할 수 있는 효과를 기대할 수 있다.
Abstract:
PURPOSE: A pharmaceutical composition containing chlorophyll isolated from soybeans is provided to ensure antioxidation and anticancer activities and to identify new physiological activity of a soybean. CONSTITUTION: A pharmaceutical composition with anticancer and antioxidation activities contains 0.1-80 wt% of chlorophyll isolated from soybeans. The chlorophyll includes chlorophyll a or chlorophyll b. The chlorophyll is separated from a soybean, sprouting bean, or bean sprout.
Abstract:
PURPOSE: An anticancer composition containing lutein isolated from green tea is provided to remove free radical groups and reactive oxygen species, and to protect DNA in a cell. CONSTITUTION: An anticancer composition contains lutein isolated from green tea. Lutein is free lutein. The cancer is prostate cancer or breast cancer. The anticancer composition is manufactured in the form of a tablet, troches, a lozenge, water soluble or oil suspension, powder, a granule, an emulsion, a hard and soft capsule, a syrup, or an elixir.
Abstract:
PURPOSE: An anticancer composition containing a petal extract of Camellia family plants is provided to enhance antioxidation and anticancer effect and to prevent and treat various diseases. CONSTITUTION: An anticancer composition contains a petal extract of Camellia family plants. The Camellia family includes Camellia sinensis, Camellia japonica, or a mixture thereof. The mixture contains petals of Camellia sinensis and Camellia japonica in a weight ratio of 1-7:1. A food additive for preventing cancer contains the anticancer composition as an active ingredient. A health functional food for preventing cancer contains food additives.