멍게 물렁병 진단용 마커 및 프라이머 세트
    14.
    发明公开
    멍게 물렁병 진단용 마커 및 프라이머 세트 有权
    用于诊断软体综合症的标记和检测器

    公开(公告)号:KR1020130084823A

    公开(公告)日:2013-07-26

    申请号:KR1020120005701

    申请日:2012-01-18

    CPC classification number: C12Q1/6883 C12Q2531/113 C12Q2600/158

    Abstract: PURPOSE: A marker and a primer set for diagnosing soft tunic syndrome in sea squirt are provided to quickly and accurately diagnose soft tunic syndrome and to effectively prevent soft tunic syndrome in sea squirt. CONSTITUTION: A marker composition for diagnosing soft tunic syndrome in sea squirt contains a forward primer and a reverse primer with a partial sequence of one protein selected among flagellated protozoa alpha-tubulin, beta-tubulin, actin, and endo or 60S ribosomal protein L7. One or more primer sets are selected among a primer set of sequenced numbers 1 and 2, a primer set of sequence numbers 3 and 4, a primer set of sequence numbers 5 and 6, a primer set of sequence numbers 7 and 8, and a primer set of sequence numbers 9 and 10. A kit for diagnosing soft tunic syndrome in sea squirt contains the composition.

    Abstract translation: 目的:提供一种用于诊断海绵软性综合征的标记物和引物,用于快速准确地诊断软性综合征,有效预防海绵软性综合征。 构成:用于诊断海绵软性综合征的标记组合物含有正向引物和反向引物,其具有选自鞭毛原生动物α-微管蛋白,β-微管蛋白,肌动蛋白和内切或60S核糖体蛋白L7中的一种蛋白质的部分序列。 在序列号1和2的引物组,序列号3和4的引物组,序列号5和6的引物组,序列号7和8的引物组以及序列号7和8的引物组中选择一个或多个引物组 序列号9和10的引物组。用于诊断海绵软性综合征的试剂盒含有组合物。

    넙치로부터 분리 및 정제된 면역글로불린에 대한단클론항체
    15.
    发明公开
    넙치로부터 분리 및 정제된 면역글로불린에 대한단클론항체 无效
    从OLIVE FLOUNDER分离和鉴定的免疫球蛋白单克隆抗体

    公开(公告)号:KR1020080008799A

    公开(公告)日:2008-01-24

    申请号:KR1020060068534

    申请日:2006-07-21

    CPC classification number: C07K16/18 C07K1/22 G01N33/532 G01N33/543

    Abstract: An immunoglobulin purified from Paralichthys olivaceus is provided to be used for studying about L.garvieae through further study utilized by the same and provide useful information required for developing a diagnostic marker and a vaccine. An immunoglobulin is characterized in that it is isolated and purified from Paralichthys olivaceus serum and consists of a heavy chain having an isoelectric point of 5.0-6.4 and a molecular weight of 66-68 kDa and a light chain having an isoelectric point of 4-7 and a molecular weight of 25-27 kDa. A method for preparing a monoclonal antibody against the immunoglobulin of Paralichthys olivaceus comprises the steps of: (a) after immunizing the Paralichthys olivaceus with an immunoglobulin of an animal, isolating serum of the Paralichthys olivaceus; (b) purifying immunoglobulin from the serum using protein A, mannan binding protein, or immunoaffinity column; and (c) after collecting spleens from mouse immunized by the immunoglobulin, fusing them with myeloma cells. A kit for detecting immunoglobulin of Paralichthys olivaceus comprises a solid support where a monoclonal antibody regarding the immunoglobulin of the Paralichthys olivaceus is fixed by a covalent bond or a physical absorption, at least one solution including detectable color developing agent, a buffered washing solution and a sample required for quality management.

    Abstract translation: 提供从Paralichthys olivaceus纯化的免疫球蛋白,用于通过进一步研究L.garvieae进行研究,并提供开发诊断标记和疫苗所需的有用信息。 免疫球蛋白的特征在于其从Paralichthys olivaceus血清中分离和纯化,并且由等电点为5.0-6.4,分子量为66-68kDa的重链和等电点为4-7的轻链组成 分子量为25-27 kDa。 制备针对Paralichthys olivaceus免疫球蛋白的单克隆抗体的方法包括以下步骤:(a)用动物免疫球蛋白免疫Paralichthys olivaceus后,分离橄榄属Paralichthys olivaceus的血清; (b)使用蛋白A,甘露聚糖结合蛋白或免疫亲和柱从血清纯化免疫球蛋白; 和(c)收集由免疫球蛋白免疫的小鼠的脾脏,将其与骨髓瘤细胞融合。 用于检测枸杞子的免疫球蛋白的试剂盒包括固体支持物,其中关于通过共价键或物理吸收固定缬氨酸Paralichthys的免疫球蛋白的单克隆抗体,至少一种溶液,包括可检测的显色剂,缓冲的洗涤溶液和 样品需要质量管理。

    어류 바이러스성 출혈성 패혈증에 대한 예방 또는 치료용 DNA 백신

    公开(公告)号:KR101863335B1

    公开(公告)日:2018-05-31

    申请号:KR1020160101563

    申请日:2016-08-10

    Abstract: 본발명은 DDx41 유전자및 VHS 바이러스당단백질유전자를포함하는재조합발현벡터를유효성분으로하는어류의바이러스성출혈성패혈증의예방또는치료용 DNA 백신조성물에관한것이다. 본발명의 DNA 백신은분자보조제로서 DDx41 유전자를사용하였고, 바이러스항원으로서는 VHSV 당단백질유전자를사용하여상기 DNA 백신을넙치치어에접종한결과높은방어율을보임을확인하였다. 따라서, 본발명의 DNA 백신은 VHS 바이러스에대한분자보조제를첨가하여만든효과적인 DNA 백신으로써, 어류, 특히넙치의바이러스성출혈성패혈증감염에대한예방및 치료용백신으로유용하게이용될수 있을것으로기대된다.

    어류 바이러스성 출혈성 패혈증에 대한 예방 또는 치료용 DNA 백신
    18.
    发明公开
    어류 바이러스성 출혈성 패혈증에 대한 예방 또는 치료용 DNA 백신 有权
    用于预防和治疗鱼病毒性出血性败血症的DNA DNA疫苗

    公开(公告)号:KR1020180017554A

    公开(公告)日:2018-02-21

    申请号:KR1020160101563

    申请日:2016-08-10

    Abstract: 본발명은 DDx41 유전자및 VHS 바이러스당단백질유전자를포함하는재조합발현벡터를유효성분으로하는어류의바이러스성출혈성패혈증의예방또는치료용 DNA 백신조성물에관한것이다. 본발명의 DNA 백신은분자보조제로서 DDx41 유전자를사용하였고, 바이러스항원으로서는 VHSV 당단백질유전자를사용하여상기 DNA 백신을넙치치어에접종한결과높은방어율을보임을확인하였다. 따라서, 본발명의 DNA 백신은 VHS 바이러스에대한분자보조제를첨가하여만든효과적인 DNA 백신으로써, 어류, 특히넙치의바이러스성출혈성패혈증감염에대한예방및 치료용백신으로유용하게이용될수 있을것으로기대된다.

    Abstract translation: 本发明涉及一种基因及DDx41预防或DNA疫苗组合物用于治疗的重组表达载体的,鱼的病毒性出血性败血症为包括基因每次VHS病毒的活性成分。 本发明的DNA疫苗作为分子佐剂DDx41基因,病毒抗原被确认为结果表明使用该基因每VHSV DNA疫苗到大比目鱼鱼苗高ERA接种。 因此,本发明作为一种有效的DNA疫苗的DNA疫苗通过加入VHS病毒的分子佐剂创建,预期有用地作为鱼,病毒性出血性败血症的预防和对感染的治疗性疫苗的特别比目鱼。

    멍게 방제 시스템
    20.
    发明公开
    멍게 방제 시스템 有权
    HALOCYNTHIA RORETZI病虫害控制系统

    公开(公告)号:KR1020130049341A

    公开(公告)日:2013-05-14

    申请号:KR1020110114302

    申请日:2011-11-04

    Inventor: 정태성

    CPC classification number: Y02A40/81 A01K61/00 A01K63/006 A01K63/042 A61L2/16

    Abstract: PURPOSE: A Halocynthia roretzi protection system is provided to effectively prevent parasite and bacteria causing Halocynthia roretzi diseases. CONSTITUTION: A Halocynthia roretzi protection system comprises: a supply pipe(100) with a spray hole; an air pump(200) which guides air to the supply pipe; a medicine case(300) containing a medicine; a medicine pump(400) which guides the medicine to the supply pipe; and a control unit(500) which controls driving of the air pump and the medicine pump. The control unit controls mixture ratio of the medicine and air and has an operation panel which controls administration time of the medicine.

    Abstract translation: 目的:提供一种Halocynthia roretzi保护系统,有效预防寄生虫和细菌引起的Halocynthia roretzi病。 构成:卤虫属保护系统包括:具有喷孔的供应管(100); 空气泵(200),其将空气引导到所述供给管; 含有药物的药盒(300) 将药剂引导到供给管的药泵(400) 以及控制空气泵和药剂泵的驱动的控制单元(500)。 控制单元控制药物和空气的混合比例,并具有控制药剂的给药时间的操作面板。

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