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    넙치 미세위성마커를 이용한 개체식별 및 친자확인 방법
    11.
    发明公开
    넙치 미세위성마커를 이용한 개체식별 및 친자확인 방법 有权
    使用橄榄石中的微生物标记鉴定和亲本的方法

    公开(公告)号：KR1020090069898A

    公开(公告)日：2009-07-01

    申请号：KR1020070137720

    申请日：2007-12-26

    Applicant: 대한민국(관리부서:국립수산과학원)

    Inventor： 김경길 ,  김종현 ,  이정호 ,  노재구 ,  김현철 ,  김우진 ,  강정하

    IPC: C12Q1/68 ,  C12N15/10 ,  C12N15/09

    CPC classification number: C12Q1/6876 ,  C12Q1/6827 ,  C12Q2527/101 ,  C12Q2563/107 ,  C12Q2600/124

    Abstract: An analysis method of genotype of each olive flounder is provided to amplify and analyze eight microsatellite markers of olive flounder and shorten analysis time and expense. A composition which specifically amplifies microsatellite markers of olive flounder of the sequence numbers 1-8 comprises primer pairs of oligonucleotide having sequences 9 and 10, 11 and 12, 13 and 14, 15 and 16, 17 and 18, 19 and 20, 21 and 22, 23 and 24 which specifically amplifies the sequence numbers 1-8. A method for identifying an individual of olive flounder comprises: a step of isolating DNA sample of fish; a step of amplifying the DNA sample using the composition which amplifies the microsatellite marker of olive flounder; and a step of analyzing band pattern of amplified product. A method for identifying parentage of olive flounder comprises: a step of isolating DNA sample of fishes; a step of amplifying the DNA sample; a step of analyzing the band pattern of amplified product; and a step of comparing the band pattern with parental generation fishes.

    Abstract translation: 提供了每个橄榄比目鱼基因型分析方法，以扩增和分析八个橄榄比目鱼的微卫星标记物，缩短分析时间和费用。 特异性扩增序列号1-8的橄榄比目鱼的微卫星标记物的组合物包括具有序列9和10,11和12,13和14,15和16,17和18,19和20,21的寡核苷酸的引物对， 22,23和24，其具体放大序列号1-8。 用于鉴定橄榄比目鱼的个体的方法包括：分离鱼的DNA样品的步骤; 使用放大橄榄比目鱼的微卫星标记物的组合物来扩增DNA样品的步骤; 以及分析扩增产物的带状图案的步骤。 用于鉴定橄榄比目鱼的亲本的方法包括：分离鱼类DNA样品的步骤; 扩增DNA样品的步骤; 分析扩增产物的带状图案的步骤; 以及将带状图案与亲代鱼类进行比较的步骤。

    신규 바실러스속 프로바이오틱스 균주
    17.
    发明公开
    신규 바실러스속 프로바이오틱스 균주 有权
    一个新的BACILLUS SP。 PROBIOTICS STRAIN

    公开(公告)号：KR1020120052667A

    公开(公告)日：2012-05-24

    申请号：KR1020100113932

    申请日：2010-11-16

    Applicant: 대한민국(관리부서:국립수산과학원) ,  씨제이제일제당 (주)

    Inventor： 김영옥 ,  김경길 ,  남보혜 ,  이상준 ,  공희정 ,  김우진 ,  김봉석 ,  김동균 ,  한현섭 ,  양시용

    IPC: C12N1/20 ,  A23K1/165 ,  A23K1/18 ,  C12R1/07

    CPC classification number: A61K35/742 ,  A23K10/18 ,  A23K50/80 ,  C12R1/07

    Abstract: PURPOSE: A novel Bacillus strain with a probiotic activity is provided to ensure antibacterial activity to fish pathogenic bacteria and to secrete useful enzymes. CONSTITUTION: A novel Bacillus 2-4(KCCM11107P) strain has a probiotic activity. A probiotic formulation contains the strain as an active ingredient. A feed or feed additive for cultivating fishes and/or crustaceans contains the strain as an active ingredient. A method for preventing mass mortality caused by pathogen of cultured fishes and/or crustaceans comprises a step of feeding the feed to the fishes and/or crustaceans. The pathogen includes Edwardsiella tarda, Streptococcus iniae, Vibrio anguillarum, Vibrio harveyi or Streptococcus parauberis.

    Abstract translation: 目的：提供一种具有益生菌活性的新型芽孢杆菌菌株，以确保对病原菌的抗菌活性，并分泌有用的酶。 构成：一种新型的芽孢杆菌2-4（KCCM11107P）菌株具有益生菌活性。 益生菌制剂含有作为活性成分的菌株。 用于培养鱼类和/或甲壳类动物的饲料或饲料添加剂含有该菌株作为活性成分。 用于预防由鱼类和/或甲壳类动物的病原体引起的大量死亡的方法包括将饲料喂入鱼类和/或甲壳类动物的步骤。 病原体包括Edwardsiella tarda，Streptococcus iniae，Vibrio anguillarum，Vibrio harveyi或Streptococcus parauberis。

    넙치 유래의 항균성 펩타이드인 베타디펜신을 암호화하는 신규한 유전자 및 그의 용도
    18.
    发明公开
    넙치 유래의 항균성 펩타이드인 베타디펜신을 암호화하는 신규한 유전자 및 그의 용도 无效
    新型基因编码橄榄球的β-缺陷及其用途

    公开(公告)号：KR1020110071057A

    公开(公告)日：2011-06-28

    申请号：KR1020110057469

    申请日：2011-06-14

    Applicant: 대한민국(관리부서:국립수산과학원)

    Inventor： 남보혜 ,  김영옥 ,  공희정 ,  김우진 ,  이상준 ,  김경길

    IPC: C07K14/46 ,  C12N15/12 ,  A61P31/00

    Abstract: PURPOSE: A novel gene encoding Paralichthys olivaceus-derived beta-defensin is provided to ensure high antibacterial activity and to develop natural antibiotics. CONSTITUTION: A Paralichthys olivaceus-derived antibacterial peptide is a polymepeptide, mutant thereof, or active fragment thereof. The polypeptide is selected from: a polypeptide encoded by cDNA having one of nucleic acids of sequence number s 1-5; a polypeptide encoding a polynucleotide which is hybridized with cDNA having the one of nucleic acids of sequence numbers 1-5; a polypeptide encoded by gDNA having one or nucleic acids of sequence numbers 6-10; a polypeptide encoding the polynucleotide hybridized with the gDNA; or a polypeptide having an amino acid among amino acid sequences of sequence numbers 11-15.

    Abstract translation: 目的：提供一种编码Paralichthys olivaceus来源的β-防御素的新基因，以确保高抗菌活性和开发天然抗生素。 构成：来自Paralichthys olivaceus的抗菌肽是多肽，其突变体或其活性片段。 所述多肽选自：由具有序列号为1-5的核酸之一的cDNA编码的多肽; 编码与序列号为1-5的核酸的cDNA杂交的多核苷酸的多肽; 由具有序列号6-10的一个或核酸的gDNA编码的多肽; 编码与所述gDNA杂交的多核苷酸的多肽; 或在序列号11-15的氨基酸序列中具有氨基酸的多肽。

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