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公开(公告)号：KR101909962B1
公开(公告)日：2018-10-22
申请号：KR1020160151765
申请日：2016-11-15
Applicant: 대한민국(관리부서:국립수산과학원)
Abstract: 본발명은바이러스성출혈성패혈증바이러스(Viral Hemorrhagic Septicemia Virus, VHSV)의 NV(non virion) 유전자와특이적으로결합하는 PNA 프로브및 이의융해패턴분석을이용한 VHSV의판별및 돌연변이를확인하는방법에관한것으로, 수산생물전염병원인바이러스의변이를간단ㆍ신속ㆍ정확하게판별하고, 어류의상기바이러스의감염여부를검출할수 있고 NV 유전자의변이를확인하는효과가있다.
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12.发明授权
公开(公告)号：KR101655071B1
公开(公告)日：2016-09-07
申请号：KR1020160052896
申请日：2016-04-29
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: C12Q1/70
CPC classification number: C12Q1/701 , C12Q2525/107 , C12Q2561/101 , C12Q2600/16
Abstract: 본발명은참돔이리도바이러스판별용유전자마커, 및이를이용한원인바이러스의판별방법에관한것으로, 더욱자세하게는참돔이리도바이러스병의원인바이러스인참돔이리도바이러스(Red Sea Bream Iridovirus, RSIV)의특정유전자를코딩하는 DNA 염기서열을선정하여증폭시킨다음, 증폭산물을특이적으로인식하는펩티드핵산(Peptide Nucleic Acid, PNA)을혼성화시키고, 혼성화된산물의온도조절을통해온도별융해곡선을얻고, 상기얻은융해곡선의분석을통해융해온도로부터참돔이리도바이러스를판별하거나어류의상기바이러스의감염여부를판별하는방법에관한것이다.
Abstract translation: 本发明涉及用于鉴别红鲷鱼痘病毒（RISV）的遗传标记，以及通过使用它们鉴别病原性病毒的方法。 更具体地，该方法包括：选择和扩增用于编码作为RSIV疾病的致病病毒的特定RSIV的DNA碱基序列; 杂交肽核酸（PNA），其特异性识别扩增产物; 通过调节杂交产物的温度，通过温度获得熔融曲线; 并通过分析获得的熔解曲线来区分病毒的RSIV或区分感染的熔解温度。
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13.发明授权
公开(公告)号：KR101644776B1
公开(公告)日：2016-08-04
申请号：KR1020160052907
申请日：2016-04-29
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: C12Q1/70
CPC classification number: C12Q1/701 , C12Q2525/107 , C12Q2561/101
Abstract: 본발명은참돔이리도바이러스검출용유전자마커, 및이를이용한원인바이러스의검출방법에관한것으로, 더욱자세하게는참돔이리도바이러스병의원인바이러스인참돔이리도바이러스(Red Sea Bream Iridovirus, RSIV)와감염성비장및 신장괴사바이러스(Infectious Spleen and Kidney Necrosis Virus, ISKNV)를구별할수 있도록, RSIV와 RSKNV 중 RSIV 특이적인특정유전자를코딩하는 DNA 염기서열을선정하여증폭시킨다음, 증폭산물을특이적으로인식하는펩티드핵산(Peptide Nucleic Acid, PNA)을혼성화시키고, 혼성화된산물의온도조절을통해온도별융해곡선을얻고, 상기얻은융해곡선의분석을통해융해온도로부터바이러스유형을판별하거나어류의상기바이러스유형의감염여부를검출하는방법에관한것이다.
Abstract translation: 本发明涉及一种用于检测红色鲷鱼虹鳟鱼病毒（RSIV）的遗传标记物，以及通过使用该病毒检测病原体病毒的方法。 更具体地，为了区分作为病原病毒的RSIV和感染性脾脏和肾坏死病毒（ISKNV），选择并扩增用于编码RSIV和RSKNV中RSIV特异性的特定基因的DNA碱基序列。 混合用于特异性识别扩增的副产物的肽核酸（PNA），通过控制混合副产物的温度获得熔解曲线。 通过分析获得的熔解曲线来检测病毒类型是从融化温度或病毒类型的感染来检测的。
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公开(公告)号：KR1020110136515A
公开(公告)日：2011-12-21
申请号：KR1020100056588
申请日：2010-06-15
Applicant: 대한민국(관리부서:국립수산과학원)
Abstract: PURPOSE: An inactivated vaccine for preventing vibrio harveyi infection, containing vibrio harveyi HN strain as an antigen is provided to reduce mortality of fishes and to enhance productivity of fishes. CONSTITUTION: A Vibrio harveyi HN(deposit number KCTC 11705BP) strain is isolated from flatfishes with vibrio harveyi infection. An inactivated vaccine for preventing vibrio herveyi infection contains the Vibrio harveyi HN(deposit number KCTC 11705BP) strain as an antigen. The vaccine further contains inactivated Streptococcus parauberis strain. A method for manufacturing the vaccine comprises a step of culturing the Vibrio harveyi HN(deposit number KCTC 11705BP) strain and inactivating. The medium for culturing the strain contains 50-200 umol of 2,2'-dipyridyl.
Abstract translation: 目的：提供一种用于预防弧菌弧菌感染的灭活疫苗，其包含哈氏弧菌HN菌株作为抗原，以减少鱼类的死亡率并提高鱼类的生产力。 构成：哈氏弧菌HN（保藏号KCTC 11705BP）菌株与哈氏弧菌感染的扁平鱼类分离。 用于预防弧菌感染感染的灭活疫苗含有作为抗原的Vibrio harveyi HN（保藏号KCTC 11705BP）菌株。 该疫苗还含有失活的欧洲白au链球菌菌株。 制备疫苗的方法包括培养哈氏弧菌HN（保藏号KCTC 11705BP）菌株并灭活的步骤。 用于培养菌株的培养基含有50-200μmol的2,2'-联吡啶。
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公开(公告)号：KR1020070088433A
公开(公告)日：2007-08-29
申请号：KR1020070080555
申请日：2007-08-10
Applicant: 대한민국(관리부서:국립수산과학원)
CPC classification number: A61K39/092 , A61K39/40 , Y10S424/827
Abstract: A method for preparing a mixture vaccine is provided to obtain the vaccine which is able to prevent Edwardsiellosis and beta-hemolysis streptococcal disease at the same time by one shot injection so that the health fish can be maintained. The method comprises the steps of: (a) after adding 20% glycerin to Edwardsiella tarda FP2018 and Streptococcus iniae JSL 0208, preserving it at a temperature of -85 deg.C; (b) after inoculating the preserved Edwardsiella tarda FP2018 and Streptococcus iniae JSL 0208 in a BHI(brain heart infusion) solid culture medium in which 1% of NaCl is added, culturing it at a temperature of 25 deg.C for 18-24 hours; (c) after respectively suspending cultured strains in sterile saline solution to prepare a suspended solution having the absorbance of 0.5, inoculating 5 ml of each of the obtained solution to 1 liter of a BHI liquid culture medium in which 1% of NaCl is added; (d) culturing each of the strains-inoculated each Erlenmeyer flask with mixing it in a shaking incubator at a temperature of 25 deg.C with the speed of 150 rotation per minute for 24 hours; (e) after respectively putting 37% formaldehyde in each of the strain culture solution to have the formaldehyde concentration of 0.4% and leaving it at room temperature for 1 hour, leaving it at 4 deg.C for 24 hours to inactivate each of the strains; (f) after centrifuging each of the inactivated strain culture solution with the speed of 6,000 rotation per minute for 20 minutes and measuring wet weight thereof, diluting it in sterile saline solution in which 0.02% of sodium azide is added to have the wet weight of 100mg/ml and storing it at a temperature of 4 deg.C; and (g) mixing a vaccine for Edwardsiellosis and a vaccine for beta-hemolysis Streptococcal disease in a ratio of 1:1.
Abstract translation: 提供一种制备混合疫苗的方法，以获得能够通过一次注射同时预防爱德华氏病和β-溶血性链球菌疾病的疫苗，从而保持健康的鱼类。 该方法包括以下步骤：（a）在Edwardsiella tarda FP2018和Streptococcus iniae JSL 0208中加入20％甘油后，在-85℃的温度下保存; （b）在保存的Edwardsiella tarda FP2018和Streptococcus iniae JSL 0208的BHI（脑心浸液）固体培养基中接种1％的NaCl后，在25℃的温度下培养18-24小时 ; （c）分别将培养菌株悬浮于无菌盐水溶液中以制备吸光度为0.5的悬浮液，将所得溶液5ml接种于1升加入1％NaCl的BHI液体培养基中; （d）将培养各菌株接种的每个锥形瓶，在振荡培养箱中在25℃的温度下以150转/分钟的速度混合24小时; （e）分别在每个菌株培养液中分别加入37％的甲醛使甲醛浓度为0.4％，并在室温下放置1小时，在4℃下放置24小时，使每个菌株失活 ; （f）以每分钟6,000转/分钟的速度离心每个灭活的菌株培养液20分钟并测量其湿重，将其稀释在无水盐水溶液中，其中加入0.02重量％的叠氮化钠以使湿重 100mg / ml，储存温度为4℃; 和（g）以1：1的比例混合用于爱德华氏病的疫苗和用于β-溶血性链球菌疾病的疫苗。
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Patent Agency Ranking
公开(公告)号：KR1020060065192A
公开(公告)日：2006-06-14
申请号：KR1020040103965
申请日：2004-12-10
Applicant: 대한민국(관리부서:국립수산과학원)
CPC classification number: C07K14/005 , A61K39/12 , A61K2039/523 , A61K2039/54 , A61K2039/552 , C12N2710/00022 , C12N2710/00034
Abstract: 본 발명은 한국형 돌돔 이리도바이러스의 면역원성을 갖는 단백질을 암호하는 유전자, 상기 유전자에 의해 암호되는 단백질, 상기 단백질에 의해 유래되는 합성 펩타이드, 상기 유전자를 포함하는 재조합 발현 벡터, 상기 재조합 벡터를 포함하는 형질전환체, 상기 형질전환체를 불활성화시켜 제조한 불활성화 백신 및 상기 백신을 이용하여 어류를 면역시키는 방법에 관한 것이다.
돌돔 이리도바이러스, 불활성화 백신-
公开(公告)号：KR102239384B1
公开(公告)日：2021-04-12
申请号：KR1020200145558
申请日：2020-11-03
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: C12Q1/70
Abstract: 본발명은 2종의뱀장어병원성바이러스의검출을위한프라이머조성물및 이를포함하는진단키트에관한것으로, 보다상세하게는뱀장어병원성바이러스인뱀장어헤르페스바이러스(AnHV) 및뱀장어내피세포괴사증바이러스(JEECV) 2종을신속하고정확하게검출할수 있는프라이머조성물및 이를포함하는진단키트에관한것이다.
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公开(公告)号：KR102136892B1
公开(公告)日：2020-07-22
申请号：KR1020200034535
申请日：2020-03-20
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: A61K31/27 , A61K31/4184 , A61K31/437 , A61K31/7048 , A61P33/10 , A23K20/105 , A23K20/137 , A23K20/132 , A23K20/163 , A23K50/80
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公开(公告)号：KR101655069B1
公开(公告)日：2016-09-07
申请号：KR1020160052902
申请日：2016-04-29
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: C12Q1/70
CPC classification number: C12Q1/701 , C12Q2525/107 , C12Q2561/101 , C12Q2600/16
Abstract: 본발명은잉어허피스바이러스그레이에스피에이치(HSV Gray Sph) 판별용유전자마커, 및이를이용한원인바이러스의판별방법에관한것으로, 더욱자세하게는잉어허피스바이러스그레이에스피에이치(HSV Gray Sph)의특정유전자를코딩하는 DNA 염기서열을선정하여증폭시킨다음, 증폭산물을특이적으로인식하는펩티드핵산(Peptide Nucleic Acid, PNA)을혼성화시키고, 혼성화된산물의온도조절을통해온도별융해곡선을얻고, 상기얻은융해곡선의분석을통해융해온도로부터잉어허피스바이러스그레이에스피에이치(HSV Gray Sph)를판별하거나어류의상기바이러스의감염여부를판별하는방법에관한것이다.
Abstract translation: 本发明涉及用于鉴别锦鲤疱疹病毒灰色SPH（HSV灰色SPH）的遗传标记，以及通过使用它们鉴别病原性病毒的方法。 更具体地说，该方法包括：选择和扩增用于编码HSV灰色SPH的特定基因的DNA碱基序列; 杂交肽核酸（PNA），其特异性识别扩增产物; 通过调节杂交产物的温度，通过温度获得熔融曲线; 并通过分析获得的熔解曲线来鉴别HSV灰色SPH或鉴别病毒感染鱼与熔解温度。
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