中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

23 records (took 0.023 seconds)

    어류의 비브리오 하베이 감염증 예방용 불활성화 백신, 그 제조방법 및 투여방법
    1.
    发明公开
    어류의 비브리오 하베이 감염증 예방용 불활성화 백신, 그 제조방법 및 투여방법 有权
    灭活疫苗对血液中的VIBRIO HARVEYI感染性疾病，其制备方法及其施用方法

    公开(公告)号：KR1020110136515A

    公开(公告)日：2011-12-21

    申请号：KR1020100056588

    申请日：2010-06-15

    Applicant: 대한민국(관리부서:국립수산과학원)

    Inventor： 김명석 ,  박명애 ,  서정수 ,  권문경 ,  김진우 ,  조미영

    IPC: C12N1/20 ,  A61K39/02 ,  A61P31/00 ,  C12R1/63

    Abstract: PURPOSE: An inactivated vaccine for preventing vibrio harveyi infection, containing vibrio harveyi HN strain as an antigen is provided to reduce mortality of fishes and to enhance productivity of fishes. CONSTITUTION: A Vibrio harveyi HN(deposit number KCTC 11705BP) strain is isolated from flatfishes with vibrio harveyi infection. An inactivated vaccine for preventing vibrio herveyi infection contains the Vibrio harveyi HN(deposit number KCTC 11705BP) strain as an antigen. The vaccine further contains inactivated Streptococcus parauberis strain. A method for manufacturing the vaccine comprises a step of culturing the Vibrio harveyi HN(deposit number KCTC 11705BP) strain and inactivating. The medium for culturing the strain contains 50-200 umol of 2,2'-dipyridyl.

    Abstract translation: 目的：提供一种用于预防弧菌弧菌感染的灭活疫苗，其包含哈氏弧菌HN菌株作为抗原，以减少鱼类的死亡率并提高鱼类的生产力。 构成：哈氏弧菌HN（保藏号KCTC 11705BP）菌株与哈氏弧菌感染的扁平鱼类分离。 用于预防弧菌感染感染的灭活疫苗含有作为抗原的Vibrio harveyi HN（保藏号KCTC 11705BP）菌株。 该疫苗还含有失活的欧洲白au链球菌菌株。 制备疫苗的方法包括培养哈氏弧菌HN（保藏号KCTC 11705BP）菌株并灭活的步骤。 用于培养菌株的培养基含有50-200μmol的2,2'-联吡啶。

    어류의 에드와드병과 베타 용혈성 연쇄구균증에 대한혼합백신 및 그 제조방법
    2.
    发明公开
    어류의 에드와드병과 베타 용혈성 연쇄구균증에 대한혼합백신 및 그 제조방법 有权
    用于EDWARDSIELLISIS和β - 溶血性结膜疾病的混合疫苗及其制造方法

    公开(公告)号：KR1020070088433A

    公开(公告)日：2007-08-29

    申请号：KR1020070080555

    申请日：2007-08-10

    Applicant: 대한민국(관리부서:국립수산과학원)

    Inventor： 김명석 ,  김진우 ,  김이청 ,  도정완 ,  조미영 ,  정승희 ,  오윤경

    IPC: A61K39/09 ,  A61K39/40

    CPC classification number: A61K39/092 ,  A61K39/40 ,  Y10S424/827

    Abstract: A method for preparing a mixture vaccine is provided to obtain the vaccine which is able to prevent Edwardsiellosis and beta-hemolysis streptococcal disease at the same time by one shot injection so that the health fish can be maintained. The method comprises the steps of: (a) after adding 20% glycerin to Edwardsiella tarda FP2018 and Streptococcus iniae JSL 0208, preserving it at a temperature of -85 deg.C; (b) after inoculating the preserved Edwardsiella tarda FP2018 and Streptococcus iniae JSL 0208 in a BHI(brain heart infusion) solid culture medium in which 1% of NaCl is added, culturing it at a temperature of 25 deg.C for 18-24 hours; (c) after respectively suspending cultured strains in sterile saline solution to prepare a suspended solution having the absorbance of 0.5, inoculating 5 ml of each of the obtained solution to 1 liter of a BHI liquid culture medium in which 1% of NaCl is added; (d) culturing each of the strains-inoculated each Erlenmeyer flask with mixing it in a shaking incubator at a temperature of 25 deg.C with the speed of 150 rotation per minute for 24 hours; (e) after respectively putting 37% formaldehyde in each of the strain culture solution to have the formaldehyde concentration of 0.4% and leaving it at room temperature for 1 hour, leaving it at 4 deg.C for 24 hours to inactivate each of the strains; (f) after centrifuging each of the inactivated strain culture solution with the speed of 6,000 rotation per minute for 20 minutes and measuring wet weight thereof, diluting it in sterile saline solution in which 0.02% of sodium azide is added to have the wet weight of 100mg/ml and storing it at a temperature of 4 deg.C; and (g) mixing a vaccine for Edwardsiellosis and a vaccine for beta-hemolysis Streptococcal disease in a ratio of 1:1.

    Abstract translation: 提供一种制备混合疫苗的方法，以获得能够通过一次注射同时预防爱德华氏病和β-溶血性链球菌疾病的疫苗，从而保持健康的鱼类。 该方法包括以下步骤：（a）在Edwardsiella tarda FP2018和Streptococcus iniae JSL 0208中加入20％甘油后，在-85℃的温度下保存; （b）在保存的Edwardsiella tarda FP2018和Streptococcus iniae JSL 0208的BHI（脑心浸液）固体培养基中接种1％的NaCl后，在25℃的温度下培养18-24小时 ; （c）分别将培养菌株悬浮于无菌盐水溶液中以制备吸光度为0.5的悬浮液，将所得溶液5ml接种于1升加入1％NaCl的BHI液体培养基中; （d）将培养各菌株接种的每个锥形瓶，在振荡培养箱中在25℃的温度下以150转/分钟的速度混合24小时; （e）分别在每个菌株培养液中分别加入37％的甲醛使甲醛浓度为0.4％，并在室温下放置1小时，在4℃下放置24小时，使每个菌株失活 ; （f）以每分钟6,000转/分钟的速度离心每个灭活的菌株培养液20分钟并测量其湿重，将其稀释在无水盐水溶液中，其中加入0.02重量％的叠氮化钠以使湿重 100mg / ml，储存温度为4℃; 和（g）以1：1的比例混合用于爱德华氏病的疫苗和用于β-溶血性链球菌疾病的疫苗。

    양식어류의 수생균 및 어병세균 구제방법
    8.
    发明授权
    양식어류의 수생균 및 어병세균 구제방법 有权
    在鱼类中排除水分和鱼类致病菌的方法

    公开(公告)号：KR100831354B1

    公开(公告)日：2008-05-28

    申请号：KR1020070056882

    申请日：2007-06-11

    Applicant: 대한민국(관리부서:국립수산과학원)

    Inventor： 지보영 ,  정승희 ,  김진우 ,  서정수

    IPC: A01N65/08

    CPC classification number: A01N65/08

    Abstract: A method for controlling aquatic fungi and fish pathogenic bacteria of cultivated fish is provided to improve productivity of the cultivated fish by effectively controlling the aquatic fungi and fish pathogenic bacteria using a proper amount of an extract of Opuntia ficus-indica var. saboten Makino and provide safe aquatic products to consumers by using environment-friendly and harmless Opuntia ficus-indica var. saboten Makino extract. To exterminate aquatic fungi of fish eggs, the fish eggs are treated with 50-80 ppm of a hydrolysis fraction of Opuntia ficus-indica var. saboten Makino for 30 minutes every 3 days until the fish eggs become germinated egg. To exterminate aquatic fungi of fish, the fish is treated with 200 ppm of the hydrolysis fraction of Opuntia ficus-indica var. saboten Makino for 2 hours per day one or two times. To treat and prevent fish pathogenic bacteria of cultivated fish, 0.1% hydrolysis fraction of Opuntia ficus-indica var. saboten Makino is absorbed into an expansion pellet and the expansion pellet is administered into the cultivated fish for 4 weeks.

    Abstract translation: 提供了一种控制养殖鱼类水生真菌和鱼类病原菌的方法，通过使用适量的榕果芸苔提取物有效控制水生真菌和鱼类病原菌，提高培养鱼的生产力。 通过使用环保无害的仙人掌榕树变种，向消费者提供安全的水产品。 saboten Makino提取物。 为了灭绝鱼卵的水生真菌，鱼卵用50-80ppm的榕属梧桐变种的水解部分处理。 saboten牧野30分钟每3天，直到鱼卵成为发芽的鸡蛋。 为了灭绝鱼类的水生真菌，用200ppm的榕果芸苔的水解分数处理鱼。 saboten牧野每天2小时一两次。 治疗和预防培养鱼的鱼类致病菌，0.1％水果分数的仙人掌榕树变种。 saboten Makino被吸收到膨胀丸中，并且将膨胀丸投入培养的鱼中4周。

    멍게 물렁증 기생충의 유전자 및 멍게 물렁증 진단 방법
    9.
    发明公开
    멍게 물렁증 기생충의 유전자 및 멍게 물렁증 진단 방법 有权
    天竺葵引起死亡的基因及其诊断方法

    公开(公告)号：KR1020070085012A

    公开(公告)日：2007-08-27

    申请号：KR1020060098722

    申请日：2006-10-11

    Applicant: 대한민국(관리부서:국립수산과학원)

    Inventor： 최동림 ,  김진우 ,  박명애 ,  지보영 ,  정준기 ,  이형호 ,  권정노 ,  최혜승 ,  임영수 ,  허영백 ,  윤호동 ,  김윤

    IPC: A01K67/033

    CPC classification number: C12Q1/6893 ,  A01K67/0336 ,  C12Q2600/158

    Abstract: A gene of a parasite causing death of Halocynthia roretzi and a method for diagnosing a parasite are provided to obtain a living organism that causes perishing of Halocynthia roretzi, and to diagnose a parasite by using a part of 18S rDNA fraction causing perishing of Halocynthia roretzi. A method for diagnosing a parasite includes the steps of: separating the entire nucleic acid from Halocynthia roretzi; performing PCR reaction by having the separated nucleic acid as a template and having a front primer and a reverse primer having a partial sequence of 18SrDNA fraction having a nucleotide sequence of sequence number 1 as a primer pair; and checking the size of the product of the PCR reaction by gel electrophoresis and comparing the size of the aforesaids product with the size of the product that is anticipated from the used primer pair.

    Abstract translation: 提供了引起Halocynthia roretzi死亡的寄生虫的基因和用于诊断寄生虫的方法，以获得导致Halocynthia roretzi死亡的生物体，并且通过使用引起Halocynthia roretzi灭活的18S rDNA部分的一部分来诊断寄生虫。 用于诊断寄生虫的方法包括以下步骤：将全部核酸与Halocynthia roretzi分离; 通过使分离的核酸作为模板并具有前序引物和具有序列号为1的核苷酸序列的部分序列的18SrDNA部分的反向引物作为引物对进行PCR反应; 并通过凝胶电泳检查PCR反应产物的大小，并将无菌产物的大小与从使用的引物对预期的产物的大小进行比较。

Patent Agency Ranking