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公开(公告)号：KR101636966B1
公开(公告)日：2016-07-11
申请号：KR1020140024179
申请日：2014-02-28
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N5/0797 , C12N5/0735
Abstract: 본발명은배아줄기세포로부터신경교전구세포, 성상세포및 희소돌기아교세포로의분화방법에관한것이다. 본발명에따른배아줄기세포로부터신경교전구세포, 성상세포및 희소돌기아교세포로의분화방법을이용할경우, 수득된신경교전구세포의지속적인배양이가능하며, 상기신경교전구세포는성상세포및 희소돌기아교세포로의분화가가능하여이와관련된독성물질의스크리닝및 독성평가에유용하게이용될수 있다.
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公开(公告)号：KR1020150042344A
公开(公告)日：2015-04-21
申请号：KR1020130120602
申请日：2013-10-10
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N5/074 , C12N5/0775 , C12N5/10 , C12N15/867
CPC classification number: C12N5/0696 , C12N5/0018 , C12N5/0606 , C12N15/867
Abstract: 본발명은 CD105세포에역분화인자가도입된유도만능줄기세포및 이의제조방법에관한것이다. 본발명에따른유도만능줄기세포는 CD105세포에역분화인자가도입됨으로써, CD105세포에역분화인자가도입된경우보다역분화형성효율및 집락발달이우수하고, 줄기세포의마커유전자를발현하며, 내배엽, 중배엽및 외배엽으로분화가가능하여, 세포치료제로유용하게사용할수 있다.
Abstract translation: 本发明涉及具有引入的重编程因子的诱导多能干细胞的CD105-CELL及其制造方法，其中允许诱导的多能干细胞将重编程因子引入CD105细胞，因此具有突出的重编程效率 与将重编程因子引入CD105 +细胞相比较，并表达干细胞的标记基因，从而分化内胚层，中胚层和外胚层，并用作细胞处理剂。
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13.发明公开
公开(公告)号：KR1020140000810A
公开(公告)日：2014-01-06
申请号：KR1020120068128
申请日：2012-06-25
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12Q1/68 , G01N33/569 , C12Q1/04
CPC classification number: G01N33/5695 , C12Q1/04 , C12Q1/686
Abstract: The present invention relates to a detection method using magnetic nanoparticles for rapidly isolating Mycobacterium avium subsp. paratuberculosis from fecal samples and, more specifically, to a method for detecting the Mycobacterium avium subsp. paratuberculosis which isolates the Mycobacterium avium subsp. paratuberculosis with high sensitivity which can be existed in animal fecal samples using the magnetic nanoparticles to which Mycobacterium avium subsp. paratuberculosis specific antibodies are combined and rapidly and simply checks infection of the Mycobacterium avium subsp. paratuberculosis by performing polymerase chain reaction using primers which are specifically combined to DNA of the Mycobacterium avium subsp. paratuberculosis. According to the present invention, the detection method using the magnetic nanoparticles for rapidly isolating the Mycobacterium avium subsp. paratuberculosis from fecal samples can easily detect the Mycobacterium avium subsp. paratuberculosis even if small number of the Mycobacterium avium subsp. paratuberculosis is existed in biological samples such as feces and prevent contagion to different entities by isolating the infected entities from an allied army by performing the method simply and rapidly.
Abstract translation: 本发明涉及使用磁性纳米颗粒快速分离鸟分枝杆菌亚种的检测方法。 来自粪便样品的副结核病，更具体地，涉及用于检测鸟分枝杆菌亚种的方法。 分离鸟分枝杆菌亚种的副结核病 具有高灵敏度的副结核病，其可以存在于动物粪便样品中，使用鸟分枝杆菌亚种的磁性纳米颗粒。 组合结核分枝杆菌特异性抗体并快速简单地检查鸟分枝杆菌感染。 使用与鸟分枝杆菌亚种DNA的特异性组合的引物进行聚合酶链反应，来治疗副结核病。 副结核病。 根据本发明，使用磁性纳米颗粒快速分离鸟分枝杆菌亚种的检测方法。 来自粪便样本的副结核病可以很容易地检测到鸟分枝杆菌。 副结核病即使少数分枝杆菌分枝杆菌。 副结核病存在于粪便等生物样本中，通过简单，快速地进行方法，将受感染的实体与盟军分离，防止对不同实体的传染。
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14.发明公开
公开(公告)号：KR1020070079198A
公开(公告)日：2007-08-06
申请号：KR1020060009731
申请日：2006-02-01
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12Q1/68
CPC classification number: C12Q1/6883 , C12Q2600/158 , C12Q1/6837
Abstract: A DNA chip is provided to evaluate the harm to human body caused by changing of human flora, which is prepared by identifying and selecting 90 kinds of genes which significantly react to the change of the human flora in colon crypt cell of human flora-associated mice and amplifying and purifying the genes. The DNA chip for detecting specific reaction of human flora comprises at least one gene selected from a gene table(A) attached to a substrate, wherein the gene table(A) consists of Dap3, Rpa1, Ccnd2, Cdc45l, Gmnn, Cul4b, Tacc2, Bnip3l, Slc26a1, Ddx1, Cacnb3, Rpl27, Slc22a1l, Cav, Tuba4, Lrp10, Mt-1, Tiam2, Zdhhc3, Rhcg, Ipo4, 2610529I12Rik, Igj, Daf1, Il18, Tnfsf13b, Prkiri, Serping1, Eif2ak3, Mpp1, Lnx1, 4732481h14Rik, Rgs12, Dcamkl1, Mllt1, Per1, Keap1, Hdac5, Ncoa6, Crem, Crsp7, Rnf12, Alas1, Cotl11, Gstm2, Siat9, 5430437P03Rik, Purb, Col3a1, Il16, Mut, 6330590F17Rik, Srpk2, Klf3, Cited1, D230019K24Rik,, Ugcg, Au043625, Rw1-pending, Hsd17b2, 5031404N19, Tccr, Npm1, Sh3glb2, Aldh6a1, Plp, Ptgs1, Fads3, Parva, C130039O16, Epc1, CPd, Mtmr7, 4930455F23Rik, Rab6ip1, Mcpt4, Fn3k, 1110037F02Rik, 1110038M16Rik, 170012H12H17Rik, Thsd1, 98301480O20Rik, Ptgs2, Tcp11, Guca1a, Usp15, Ybx2, Tcl1, Cldn8 and Ckap2. The method comprises the steps of: (a) reacting the DNA chip with RNA which is collected from colon crypt cells and amplified; and (b) investigating the gene expression degree of the DNA chip.
Abstract translation: 提供DNA芯片以评估由人类菌群变化引起的对人体的伤害，其通过鉴定和选择与人类菌群相关小鼠的结肠隐窝细胞中的人类菌群的变化显着反应的90种基因而制备 并扩增和纯化基因。 用于检测人类菌群特异性反应的DNA芯片包含至少一种选自附着于底物的基因表（A）的基因，其中基因表（A）由Dap3，Rpa1，Ccnd2，Cdc451，Gmnn，Cul4b，Tacc2组成 ，Bnip3I，Slc26a1，Ddx1，Cacnb3，Rpl27，Slc22a1，Cav，Tuba4，Lrp10，Mt-1，Tiam2，Zdhhc3，Rhcg，Ipo4,2610529I12Rik，Igj，Daf1，Il18，Tnfsf13b，Prkiri，Serping1，Eif2ak3，Mpp1，Lnx1 ，4732481h14Rik，Rgs12，Dcamkl1，Mllt1，Per1，Keap1，Hdac5，Ncoa6，Crem，Crsp7，Rnf12，Alas1，Cotl11，Gstm2，Siat9,5430437P03Rik，Purb，Col3a1，Il16，Mut，6330590F17Rik，Srpk2，Klf3，Cited1，D230019K24Rik ，Ugcg，Au043625，Rw1-pending，Hsd17b2,5031404N19，Tccr，Npm1，Sh3glb2，Aldh6a1，Plp，Ptgs1，Fads3，Parva，C130039O16，Epc1，CPd，Mtmr7,4930455F23Rik，Rab6ip1，Mcpt4，Fn3k，1110037F02Rik，1110038M16Rik， 170012H12H17Rik，Thsd1,98301480O20Rik，Ptgs2，Tcp11，Guca1a，Usp15，Ybx2，Tcl1，Cldn8和Ckap2。 该方法包括以下步骤：（a）使DNA芯片与从结肠隐窝细胞收集的RNA进行反应并扩增; 和（b）研究DNA芯片的基因表达程度。
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公开(公告)号：KR1020150051122A
公开(公告)日：2015-05-11
申请号：KR1020140024179
申请日：2014-02-28
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N5/0797 , C12N5/0735
CPC classification number: C12N5/0618
Abstract: 본발명은배아줄기세포로부터신경교전구세포, 성상세포및 희소돌기아교세포로의분화방법에관한것이다. 본발명에따른배아줄기세포로부터신경교전구세포, 성상세포및 희소돌기아교세포로의분화방법을이용할경우, 수득된신경교전구세포의지속적인배양이가능하며, 상기신경교전구세포는성상세포및 희소돌기아교세포로의분화가가능하여이와관련된독성물질의스크리닝및 독성평가에유용하게이용될수 있다.
Abstract translation: 本发明涉及诱导胚胎干细胞将其分化成胶质祖细胞，星形胶质细胞和少突胶质细胞的方法。 根据本发明的一个实施方案，该方法包括以下步骤：1）培养胚胎干细胞以形成胚状体; 2）将胚状体分离成单个细胞并培养分离的单细胞以诱导神经干细胞的产生; 3）另外培养神经干细胞，将培养的神经干细胞分离成单细胞，将分离的神经干细胞传代培养5〜40次。 当使用根据本发明的方法时，可以连续培养获得的胶质祖细胞并诱导胶质祖细胞分化为星形胶质细胞和少突胶质细胞，从而有效地用于筛选与细胞有关的毒性物质并评估毒性 。
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Patent Agency Ranking
公开(公告)号：KR1020140082021A
公开(公告)日：2014-07-02
申请号：KR1020120150936
申请日：2012-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N5/0735 , C12N5/02
CPC classification number: C12N5/0696
Abstract: The present invention relates to a complete induced pluripotent stem cell obtained by treating sodium butyrate and a treating method thereof which contain a process of treating the sodium butyrate onto the complete induced pluripotent stem cell. According to the present invention, the production method can be effectively used for research of the relevant fields, as a partial induced pluripotent stem cell having an interval characteristic of a somatic cell and a stem cell can be efficiently converted into the complete induced pluripotent stem cell.
Abstract translation: 本发明涉及通过处理丁酸钠而获得的完全诱导的多能干细胞及其治疗方法，其包含将丁酸钠处理到完全诱导的多能干细胞上的方法。 根据本发明，生产方法可以有效地用于相关领域的研究，因为具有体细胞间隔特征的部分诱导多能干细胞和干细胞可以有效地转化为完全诱导的多能干细胞 。
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公开(公告)号：KR1020140082016A
公开(公告)日：2014-07-02
申请号：KR1020120150885
申请日：2012-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: C12N15/11 , C12Q1/6844 , C12Q1/6886 , C12Q2600/154
Abstract: The present invention relates to a marker composition for diagnosis of bone marrow cancer and a kit and a method for diagnosis of bone marrow cancer including the marker. The marker composition of the present invention amplifies a certain area where CpG area of the gene BCL11a, RB1, GLIS2 and ABI2 is included, in order to compare similarities and differences of the methylation level of the gene. Hence, the present invention can effectively be used to promptly and accurately diagnose bone marrow cancer including T-cell lymphoma, B-cell lymphoma and leukemia.
Abstract translation: 本发明涉及用于诊断骨髓癌的标记组合物和用于诊断包括该标记物的骨髓癌的试剂盒和方法。 为了比较基因的甲基化水平的相似度和差异，本发明的标记物组合物扩增了包含基因BCL11a，RB1，GLIS2和ABI2的CpG面积的某一区域。 因此，本发明可以有效地用于及时，准确地诊断包括T细胞淋巴瘤，B细胞淋巴瘤和白血病在内的骨髓癌。
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18.发明授权아플라톡신 Ｂ1에 대한 결합 특이성 및 친화성이 향상된 단클론 항체 및 이를 포함하는 아플라톡신 Ｂ1 검출용 키트 有权单克隆抗体增强了与黄曲霉毒素B1的结合的特异性和亲和力，以及用于检测黄曲霉毒素B1的黄曲霉毒素B1的试剂盒
公开(公告)号：KR101149874B1
公开(公告)日：2012-06-27
申请号：KR1020090047325
申请日：2009-05-29
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A monoclonal antibody with improved binding specificity and affinity and a kit for detecting aflatoxin B1 are provided to enhance purification efficiency and detection intensity. CONSTITUTION: A conjugate comprises aflatoxin B1, a linker, and a carrier protein. The mole ratio of aflatoxin B1 and carrier protein is 1:5.5-1:6.5, respectively. The carrier protein is Bovine Serum Albumin(BSA), KLH(keyhole limpet hemocyanin), or ovalbumin. A kit for detecting aflatoxin B1 contains the monoclonal antibody.
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19.发明公开아플라톡신 Ｂ1에 대한 결합 특이성 및 친화성이 향상된 단클론 항체 및 이를 포함하는 아플라톡신 Ｂ1 검출용 키트 有权单克隆抗体增强了与AFLATOXIN B1的结合的特异性和亲和力，以及用于检测其包含的AFLATOXIN B1的试剂盒
公开(公告)号：KR1020100128739A
公开(公告)日：2010-12-08
申请号：KR1020090047325
申请日：2009-05-29
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A monoclonal antibody with improved binding specificity and affinity and a kit for detecting aflatoxin B1 are provided to enhance purification efficiency and detection intensity. CONSTITUTION: A conjugate comprises aflatoxin B1, a linker, and a carrier protein. The mole ratio of aflatoxin B1 and carrier protein is 1:5.5-1:6.5, respectively. The carrier protein is Bovine Serum Albumin(BSA), KLH(keyhole limpet hemocyanin), or ovalbumin. A kit for detecting aflatoxin B1 contains the monoclonal antibody.
Abstract translation: 目的：提供具有改善的结合特异性和亲和力的单克隆抗体和用于检测黄曲霉毒素B1的试剂盒，以提高净化效率和检测强度。 构成：缀合物包含黄曲霉毒素B1，接头和载体蛋白。 黄曲霉毒素B1和载体蛋白的摩尔比分别为1：5.5-1：6.5。 载体蛋白是牛血清白蛋白（BSA），KLH（匙孔血蓝蛋白）或卵白蛋白。 用于检测黄曲霉毒素B1的试剂盒含有单克隆抗体。
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20.发明公开제랄레논에 대한 결합 특이성 및 친화성이 향상된 단클론 항체 및 이를 포함하는 제랄레논 검출용 키트 无效单克隆抗体增强了结合ZEARALENONE和用于检测包含其的ZEARALENON的试剂盒的特异性和亲和力
公开(公告)号：KR1020100127407A
公开(公告)日：2010-12-06
申请号：KR1020090045839
申请日：2009-05-26
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C07K14/415 , C07K14/76 , C07K16/18 , G01N33/53
Abstract: PURPOSE: A monoclonal antibody with improved binding specificity and affinity and a kit having the same for detecting zearalenone are provided to effectively remove zearalenone contamination in feed or food. CONSTITUTION: A conjugate contains zearalenone, linker, and carrier protein. The mole ratio of the zearalenone and carrier protein is 1:22-1:26. The carrier protein is bovine serum albumin. A monoclonal antibody of deposit number KCLRF-BP-00207 is produced by inputting the conjugate to a mouse. A kit for detecting KCLRF-BP-00207 contains the monoclonal antibody.
Abstract translation: 目的：提供具有改善的结合特异性和亲和性的单克隆抗体以及用于检测玉米赤霉烯酮的试剂盒，以有效去除饲料或食物中的玉米赤霉烯酮污染物。 构成：缀合物含有玉米赤霉烯酮，接头和载体蛋白。 玉米赤霉烯酮和载体蛋白的摩尔比为1:22-1:26。 载体蛋白是牛血清白蛋白。 通过将偶联物输入小鼠产生保藏号为KCLRF-BP-00207的单克隆抗体。 用于检测KCLRF-BP-00207的试剂盒含有单克隆抗体。
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