公开(公告)号：KR1020020038844A

公开(公告)日：2002-05-24

申请号：KR1020000068693

申请日：2000-11-18

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 허문 ,  조성근 ,  조동희 ,  정병열

IPC: C07K14/23 ,  G01N33/50

CPC classification number: C07K14/23 ,  C07K1/145 ,  C07K1/16 ,  G01N33/536

Abstract: PURPOSE: Provided are 8kDa antigen derived from Brucella abortus RB51, its purification method and a detection method of antibody for RB51 using the same antigen. CONSTITUTION: 8kDa antigen is derived from the surface of Brucella abortus RB51 and purified by the steps of: treating Brucella abortus RB51 with sarcosine to extract antigens from its surface; concentrating the extract with ammonium sulfate; dissolving the concentrate in triton X-100 then passing it through Superose 12HR 10/30 column; then passing the fractions through Sephacryl S-100.

Abstract translation: 目的：提供来源于流产布鲁杆菌RB51的8kDa抗原，其纯化方法和使用相同抗原的RB51抗体检测方法。 构成：8kDa抗原来源于流产布鲁杆菌RB51的表面，并通过以下步骤纯化：用肌氨酸处理布鲁氏菌流产RB51，从其表面提取抗原; 用硫酸铵浓缩提取物; 将浓缩物溶解在triton X-100中，然后将其通过Superose 12HR 10/30柱; 然后将级分通过Sephacryl S-100。

公开(公告)号：KR1020090041663A

公开(公告)日：2009-04-29

申请号：KR1020070107293

申请日：2007-10-24

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 조윤상 ,  이상은 ,  고영준 ,  조동희 ,  이향심 ,  황인영 ,  남향미 ,  허은정 ,  김종만 ,  정석찬

IPC: C07K14/35 ,  C07K1/14

Abstract: A protein with antibody specificity against Bovine Tuberculosis-infected blood serum is provided to diagnose Bovine Tuberculosis and prevent spread of the Bovine Tuberculosis by improving specificity and sensitivity against the Bovine Tuberculosis. A protein with antibody specificity against Bovine Tuberculosis-infected blood serum represents a Mycobacterium bovis protein having amino acid sequence of SEQ ID NO:1, 2 or 3. A method for purifying the protein comprises the following steps of: immersing proteins isolated from cultivated supernatant of the Mycobacterium bovis; partitioning the proteins using anion exchange resin chromatography; and selecting the proteins which are reacted to the Bovine Tuberculosis-infected blood serum.

Abstract translation: 提供具有针对牛结核病感染血清的抗体特异性的蛋白质以诊断牛结核病并通过改善对牛结核病的特异性和敏感性来预防牛结核病的传播。 具有针对牛结核病感染血清的抗体特异性的蛋白质代表具有SEQ ID NO：1,2或3的氨基酸序列的牛分枝杆菌蛋白质。纯化蛋白质的方法包括以下步骤：将从培养上清液中分离的蛋白质 的牛分枝杆菌; 使用阴离子交换树脂色谱分离蛋白质; 并选择与牛结核感染血清反应的蛋白质。