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公开(公告)号：KR100968069B1
公开(公告)日：2010-07-08
申请号：KR1020070107293
申请日：2007-10-24
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 우 결핵균 항체에 특이적으로 반응하는 단백질 및 그 정제 방법에 관한 것으로, 더욱 상세하게는 우 결핵균 단백질 중 우결핵균 감염 혈청에 대한 항체 특이 단백질 및 그 정제 방법에 관한 것이다. 본 발명은 우결핵균의 혈청학적 진단 기술을 향상시킴으로써 우결핵의 확산방지를 도모할 수 있다.
우결핵, 단백질항원, 이온교환크로마토그래피, 항체검사, 면역블롯팅-
公开(公告)号：KR1020100066652A
公开(公告)日：2010-06-18
申请号：KR1020080125050
申请日：2008-12-10
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A medium for selective culture of brucella is provided to selectively culture and isolate brucella in a short time and to research in infection analysis and dynamic study. CONSTITUTION: A medium for selective culture of brucella contains 0.01-1.0 weight% of erythritol as a carbon source. The medium additionally contains neutral red. The medium is tryptic soy agar in which dextrose is removed. The medium also contains: bacitracin or vancomycin as an antibacterial agent to gram-positive bacteria; azotrenam or nalidixic acid as an antibacterial agent to gram-negative bacteria; or amphotericine B or nystatin as an antifungal agent.
Abstract translation: 目的：提供选择性培养布鲁氏菌的培养基，以便在短时间内选择性培养和分离布鲁杆菌，并进行感染分析和动态研究。 构成：用于选择性培养布鲁氏菌的培养基含有0.01-1.0重量％的赤藓糖醇作为碳源。 该介质还含有中性红。 培养基是去除葡萄糖的胰蛋白酶大豆琼脂。 培养基还含有：杆菌肽或万古霉素作为革兰氏阳性菌的抗菌剂; azotrenam或萘啶酸作为革兰氏阴性细菌的抗菌剂; 或两性霉素B或制霉菌素作为抗真菌剂。
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公开(公告)号：KR1020120051196A
公开(公告)日：2012-05-22
申请号：KR1020100112506
申请日：2010-11-12
Applicant: 주식회사 바이오노트 , 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A kit for diagnosing bovine tuberculosis containing monoclonal antibodies and polyclonal antibodies is provided to effectively measure tuberculosis attack. CONSTITUTION: A monoclonal antibody which specifically binds to an epitope is denoted by an amino acid sequence of sequence number 8 in a bovine interferon gamma receptor-binding region. The monoclonal antibody is produced from hydridoma cells(deposit number KCTC 11784BP). A kit for diagnosing bovine contains monoclonal antibody or antigen binding fragment and anti-bovine interferon gamma polyclonal antibody.
Abstract translation: 目的：提供用于诊断含有单克隆抗体和多克隆抗体的牛结核的试剂盒，以有效测量结核病的发作。 构成：特异性结合表位的单克隆抗体由牛干扰素γ受体结合区中的序列号8的氨基酸序列表示。 单克隆抗体由Hydridoma细胞（保藏号KCTC 11784BP）产生。 用于诊断牛的试剂盒含有单克隆抗体或抗原结合片段和抗牛干扰素γ多克隆抗体。
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4.发明公开면역특이단백질 항원을 사용하는 우결핵 특이 효소면역진단키트 및 이를 이용한 우결핵 진단방법 有权包含免疫特异性蛋白质抗原和其他免疫原的BOVINE TUBCCOSOS特异性酶免疫测定试剂盒及其使用方法诊断牛血管病毒
公开(公告)号：KR1020090052175A
公开(公告)日：2009-05-25
申请号：KR1020070118743
申请日：2007-11-20
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 우결핵 특이 효소면역진단 키트 및 이를 이용한 우결핵 진단방법에 관한 것으로, 보다 상세하게는 우결핵균의 단백질항원 중 면역특이단백질 항원인 MPB70 등을 이용한 효소면역측정법을 통하여 신속하고 정확하게 우결핵을 진단하는 우결핵 특이 효소면역진단 키트 및 이를 이용한 우결핵 진단방법에 관한 것이다.
우결핵, 정제단백질, 효소면역측정법, 피내검사-
公开(公告)号：KR1020090007086A
公开(公告)日：2009-01-16
申请号：KR1020070070724
申请日：2007-07-13
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: C07K14/35 , C07K14/00 , G01N33/53 , G01N33/6854
Abstract: An immunity-specific protein antigen for diagnosis of bovine tuberculosis is provided to supply the information on the protein antigen which specifically and immunologically reacts with the blood serum of a subject infected by Mycobacterium bovis among protein antigens existing in the upper layer of the Mycobacterium bovis-cultured medium. The protein antigen is obtained from the upper layer of the cultured medium of Mycobacterium bovis which is tuberculous bacterium, comprises one or more amino acid seqeunces selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20 and SEQ ID NO:21, and raises the immune response in the blood serum of the subject infected with Mycobacterium bovis.
Abstract translation: 提供了用于诊断牛结核病的免疫特异性蛋白质抗原，以提供与牛分枝杆菌分枝杆菌上层存在的蛋白质抗原中特异性和免疫学上与牛分枝杆菌感染的受试者血清特异性和免疫反应的蛋白质抗原的信息， 培养基。 蛋白质抗原是从作为结核菌的牛分枝杆菌培养基的上层得到的，包含一个或多个选自SEQ ID NO：1，SEQ ID NO：2，SEQ ID NO：1的氨基酸序列。 3，SEQ ID NO：4，SEQ ID NO：5，SEQ ID NO：6，SEQ ID NO：7，SEQ ID NO：8，SEQ ID NO：9，SEQ ID NO：10，SEQ ID NO： SEQ ID NO：12，SEQ ID NO：13，SEQ ID NO：14，SEQ ID NO：15，SEQ ID NO：16，SEQ ID NO：17，SEQ ID NO：18，SEQ ID NO：19， NO：20和SEQ ID NO：21，并且提高感染牛分枝杆菌的受试者的血清中的免疫应答。
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Patent Agency Ranking
公开(公告)号：KR1020110052295A
公开(公告)日：2011-05-18
申请号：KR1020090109275
申请日：2009-11-12
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A method for diagnosing cattle tuberculosis using intradermal diagnostic composition is provided to easily prepare a large amount of intradermal diagnostic composition and to ensure diagnosis accuracy. CONSTITUTION: An intradermal diagnostic composition for testing cattle tuberculosis contains recombinant proteins of Esat6, HspX, PhoS, MPB70, MPB64, MPB83, Ag85, and SahH. A method for diagnosing tuberculosis of mammal except for human is performed using the intradermal diagnostic composition containing the recombinant proteins. The recombinant protein is prepared by isolating from Mycobacterium bovis and performing PCR.
Abstract translation: 目的：提供使用皮内诊断组合物诊断牛结核病的方法，以容易地制备大量皮内诊断组合物并确保诊断准确性。 构成：用于测试牛结核的皮内诊断组合物含有Esat6，HspX，PhoS，MPB70，MPB64，MPB83，Ag85和SahH的重组蛋白。 使用含有重组蛋白质的皮内诊断组合物进行用于诊断除人以外的哺乳动物结核病的方法。 通过从牛分枝杆菌中分离并进行PCR来制备重组蛋白。
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公开(公告)号：KR1020080068361A
公开(公告)日：2008-07-23
申请号：KR1020070006055
申请日：2007-01-19
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: A plate agglutination tester and a method for measuring an immuno-reaction using the same are provided to decrease error of the result analysis in accordance with proficiency of a person performing diagnosis, show the degree of various immuno-reactions in an objective value and perform various statistical analyses, thereby being very useful for measuring the immuno-reaction. A plate agglutination tester consists of a transparent film and is characterized in that a section, which consists of a plurality of circles and where an antigen-antibody agglutination reaction occurs, is arranged and formed in horizontal and vertical directions on an upper side of the film. A method for measuring an immuno-reaction comprises the steps of: (a) mixing an antigen and an antibody specifically bound to the antigen with the plate agglutination tester to induce an agglutination reaction; (b) scanning the agglutination reaction induced plate agglutination tester and converting a scanning result into an image file; and (c) analyzing the obtained image file using a computer program capable of detecting fine agglutination reactants to numberize the intensity of the agglutination reaction. Further, the transparent film is an over head project film.
Abstract translation: 提供平板凝集试验机和使用其的免疫反应测定方法，根据进行诊断的人员的熟练程度来减少结果分析的误差，以目标值显示各种免疫反应的程度，并进行各种 统计分析，因此对测量免疫反应非常有用。 板凝集试验仪由透明膜构成，其特征在于，在膜的上侧，在水平和垂直方向上布置并形成由多个圆组成并且发生抗原 - 抗体凝集反应的部分 。 测定免疫反应的方法包括以下步骤：（a）将抗原与抗原特异性结合的抗体与平板凝集试验仪混合以诱导凝集反应; （b）扫描凝集反应诱导的板凝集测试仪并将扫描结果转换为图像文件; 和（c）使用能够检测细微凝集反应物的计算机程序来分析所获得的图像文件，以对凝集反应的强度进行编号。 此外，透明膜是顶部投影膜。
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公开(公告)号：KR101263913B1
公开(公告)日：2013-05-14
申请号：KR1020100112506
申请日：2010-11-12
Applicant: 주식회사 바이오노트 , 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 소의인터페론감마에특이적으로결합하는단일클론항체및 폴리클론항체를포함하는우결핵진단키트및 이를이용한우결핵진단방법에관한것이다. 일구체예에따른소의인터페론감마에특이적으로결합하는단일클론항체, 폴리클론항체를포함하는우결핵진단키트및 이를이용한우결핵진단방법에따르면, 소과동물의우결핵발병여부를효율적으로측정할수 있다.
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公开(公告)号：KR1020090041663A
公开(公告)日：2009-04-29
申请号：KR1020070107293
申请日：2007-10-24
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: A protein with antibody specificity against Bovine Tuberculosis-infected blood serum is provided to diagnose Bovine Tuberculosis and prevent spread of the Bovine Tuberculosis by improving specificity and sensitivity against the Bovine Tuberculosis. A protein with antibody specificity against Bovine Tuberculosis-infected blood serum represents a Mycobacterium bovis protein having amino acid sequence of SEQ ID NO:1, 2 or 3. A method for purifying the protein comprises the following steps of: immersing proteins isolated from cultivated supernatant of the Mycobacterium bovis; partitioning the proteins using anion exchange resin chromatography; and selecting the proteins which are reacted to the Bovine Tuberculosis-infected blood serum.
Abstract translation: 提供具有针对牛结核病感染血清的抗体特异性的蛋白质以诊断牛结核病并通过改善对牛结核病的特异性和敏感性来预防牛结核病的传播。 具有针对牛结核病感染血清的抗体特异性的蛋白质代表具有SEQ ID NO：1,2或3的氨基酸序列的牛分枝杆菌蛋白质。纯化蛋白质的方法包括以下步骤：将从培养上清液中分离的蛋白质 的牛分枝杆菌; 使用阴离子交换树脂色谱分离蛋白质; 并选择与牛结核感染血清反应的蛋白质。
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