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公开(公告)号：KR1020090117448A
公开(公告)日：2009-11-12
申请号：KR1020080043496
申请日：2008-05-09
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A23K10/30 , A23K20/195
Abstract: PURPOSE: An antioxidant for supplementing feed using Forsythiae fructus extract and a preparation method for the Forsythiae Fructus extract are provided to show improved daily gain, feed intake, and feed efficiency compared to a control group. CONSTITUTION: An antioxidant for supplementing feed contains Forsythiae fructus extract. A method for preparing Forsythiae fructus extract comprises the following steps of: washing 100g Forsythiae fructus with water to remove dust; placing the washed Forsythiae fructus in a white bag; adding 1,000ml distilled water into the white bag to soak for 20-30 minutes; boiling down the soaked Forsythiae fructus for 150 minutes and obtaining an extract; adding another 800ml distilled water and boiling down for 150 minutes to obtain the extract; adding another 700ml distilled water and boiling down for 150 minutes to obtain the extract; collecting the extracts and concentrating until the volume becomes 100ml; and freeze-drying the concentrate.
Abstract translation: 目的：提供使用连翘果提取物补充饲料的抗氧化剂和连翘果提取物的制备方法，以显示与对照组相比改善的日增重，进食量和饲料效率。 构成：补充饲料的抗氧化剂含有连翘果提取物。 连翘果提取物的制备方法为：用水洗涤连翘100份，除去灰尘; 将洗涤的连翘属植物置于白色袋中; 在白色袋中加入1000ml蒸馏水浸泡20-30分钟; 煮熟连翘150分钟，得到提取物; 加入800ml蒸馏水，煮沸150分钟，得到提取物; 再加入700ml蒸馏水，煮沸150分钟，得到提取物; 收集提取物并浓缩直到体积变成100ml; 并冷冻干燥浓缩物。
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公开(公告)号：KR1020090093292A
公开(公告)日：2009-09-02
申请号：KR1020080018738
申请日：2008-02-29
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K36/06
Abstract: A bacteriolysis extract of Saccharomyces chevalieri is provided to increase the activation of neutrophil and enhance non-specific defense activity against the pathogenic bacteria. An immune enhancer, vaccine adjuvant, adjuvant treating agent or feed additive comprises bacteriolysis extract of Saccharomyces chevalieri as an active ingredient. A method for manufacturing a bacteriolysis extract of Saccharomyces chevalieri comprises: a step of culturing Saccharomyces chevalieri in media at 26°C for 72 hours; a step of centrifuging media and suspending in PBS; a step of adding dissolution agent and treating at room temperature for 2-3 days; a step of confirming the dissolution status with a microscope; a step of adjusting pH concentration to 6.5-7.2; and a step of drying and maintaining in a refrigerator.
Abstract translation: 提供雪花酵母的溶菌提取物以增加嗜中性粒细胞的活化并增强针对致病菌的非特异性防御活性。 免疫增强剂，疫苗佐剂，佐剂处理剂或饲料添加剂包括作为活性成分的雪花酵母的溶菌提取物。 酿酒酵母的溶菌提取物的制备方法包括：在培养基中在26℃下培养雪花酵母（Saccharomyces chevalieri）72小时的步骤; 离心培养基并悬浮于PBS中的步骤; 加入溶解剂并在室温下处理2-3天的步骤; 用显微镜确认溶出状态的步骤; 将pH浓度调节至6.5-7.2的步骤; 以及在冰箱中干燥和保持的步骤。
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公开(公告)号：KR1020090091884A
公开(公告)日：2009-08-31
申请号：KR1020080017089
申请日：2008-02-26
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K36/06
Abstract: An immune enhancer and vaccine adjuvant additive for animal, which contains Zygosaccharomyces bailii lysates and an auxiliary therapeutic agent are provided to enhance the activation of neutrophil and non-specific protection effect to attack inoculation. An immune enhancer, vaccine adjuvant additive, auxiliary therapeutic agent and feed additive comprises a Zygosaccharomyces bailii lysates. A method for manufacturing the Zygosaccharomyces bailii lysate comprises: a step of culturing the Zygosaccharomyces bailii in the media at 26‹C for 72 hours; a step of centrifuging the cultured media and suspending in phosphate buffered saline (PBS); a step of adding dissolution agent and stirring at room temperature for two to three days; a step of adjusting pH concentration to 6.5-7.2; and a step of drying and maintaining in a thermostatic chamber.
Abstract translation: 提供含有接合酵母发酵酵母裂解物和辅助治疗剂的动物免疫增强剂和疫苗佐剂添加剂，以增强中性粒细胞的活化和非特异性保护作用来攻击接种。 免疫增强剂，疫苗佐剂添加剂，辅助治疗剂和饲料添加剂包括接合酵母（Zygosaccharomyces bailii lysates）。 一种制造接合酵母发酵酵母裂解物的方法，包括：在培养基中培养接合酵母属酵母在26℃72小时的步骤; 将培养基离心并悬浮于磷酸盐缓冲盐水（PBS）中的步骤; 在室温下加入溶解剂并搅拌2〜3天的步骤; 将pH浓度调节至6.5-7.2的步骤; 以及在恒温室中干燥和保持的步骤。
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4.发明公开동물의 면역능을 증강시키는 노란 민들레 열탕 추출물, 그제법 및 용도 无效用于改善动物免疫力的UM UM AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME
公开(公告)号：KR1020090021611A
公开(公告)日：2009-03-04
申请号：KR1020070086251
申请日：2007-08-27
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K36/288 , A61P37/02
Abstract: Provided is an immunostimulant using dandelion extract which improves a function of a nonspecific defense against pathogen and increases activity of animal immunocytes such as neutrophil and macrophages. An immunostimulant using dandelion extract comprises dandelion extract obtained by a hot water process as an active ingredient. A method for preparing the immunostimulant comprises a steps of extracting the dandelion extract at a temperature of 90~100°C by hot distilled water. The dandelion extract can be used independently or with additives which are generally used.
Abstract translation: 提供使用蒲公英提取物的免疫刺激剂，其改善对病原体的非特异性防御的功能并增加动物免疫细胞如嗜中性粒细胞和巨噬细胞的活性。 使用蒲公英提取物的免疫刺激剂包含通过热水法获得的蒲公英提取物作为活性成分。 制备免疫刺激剂的方法包括以下步骤：通过热蒸馏水在90〜100℃的温度下提取蒲公英提取物。 蒲公英提取物可以单独使用或与通常使用的添加剂一起使用。
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公开(公告)号：KR1020080081499A
公开(公告)日：2008-09-10
申请号：KR1020070021570
申请日：2007-03-05
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K36/064 , C12N1/18
Abstract: An exo-polysaccharide derived from a Saccharomyces spp. strain is provided to enhance various activities of neutrophil, macrophage and lymphocyte, thereby being widely applied to an animal immunity-enhancing agent, a vaccine supplemental additive and an adjuvant therapeutic agent. An animal immunity enhancing agent comprises exo-polysaccharides extracted from a culture material of a Saccharomyces spp. strain. A method for preparing the exo-polysaccharides derived from the Saccharomyces spp. strain comprises the steps of: (a) after suspending pure colonies obtained by culturing the Saccharomyces spp. strain in sterile saline solution, inoculating 1 ml of the suspension into 1,000 ml of yeast malt broth and culturing it at a temperature of 28 deg.C for 72 hours; (b) after adding two times amount of distilled water into a culture material obtained from the step(a) and mixing it, boiling the mixture at a temperature of 100 deg.C for 1 hour and centrifuging the boiled mixture under 5,000xg for 30 minutes to collect supernatant therefrom; and (c) after adding two times amount of ethyl alcohol to the supernatant and mixing it, leaving a mixture in a cool chamber at a temperature of 4 deg.C for 2 days and collecting precipitated polysaccharides therefrom.
Abstract translation: 衍生自酵母属（Saccharomyces spp。）的外多糖。 提供菌株以增强嗜中性粒细胞，巨噬细胞和淋巴细胞的各种活性，从而广泛应用于动物免疫增强剂，疫苗补充添加剂和辅助治疗剂。 动物免疫增强剂包括从酵母属的培养物质中提取的外多糖。 应变。 一种制备衍生自酵母属（Saccharomyces spp）的外多糖的方法。 菌株包括以下步骤：（a）悬浮通过培养酵母属（Saccharomyces spp）获得的纯菌落后。 在无菌生理盐水溶液中，将1ml悬浮液接种到1,000ml酵母麦芽培养液中，并在28℃的温度下培养72小时; （b）在向步骤（a）获得的培养物中加入两倍量的蒸馏水并混合后，在100℃的温度下将混合物煮沸1小时，并将煮沸的混合物在5,000xg下离心30分钟 分钟收集上清液; 和（c）在向上清液中加入两倍量的乙醇并混合后，在4℃的温度下在冷室中留下混合物2天，并从中收集沉淀的多糖。
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Patent Agency Ranking
公开(公告)号：KR1020080068361A
公开(公告)日：2008-07-23
申请号：KR1020070006055
申请日：2007-01-19
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: A plate agglutination tester and a method for measuring an immuno-reaction using the same are provided to decrease error of the result analysis in accordance with proficiency of a person performing diagnosis, show the degree of various immuno-reactions in an objective value and perform various statistical analyses, thereby being very useful for measuring the immuno-reaction. A plate agglutination tester consists of a transparent film and is characterized in that a section, which consists of a plurality of circles and where an antigen-antibody agglutination reaction occurs, is arranged and formed in horizontal and vertical directions on an upper side of the film. A method for measuring an immuno-reaction comprises the steps of: (a) mixing an antigen and an antibody specifically bound to the antigen with the plate agglutination tester to induce an agglutination reaction; (b) scanning the agglutination reaction induced plate agglutination tester and converting a scanning result into an image file; and (c) analyzing the obtained image file using a computer program capable of detecting fine agglutination reactants to numberize the intensity of the agglutination reaction. Further, the transparent film is an over head project film.
Abstract translation: 提供平板凝集试验机和使用其的免疫反应测定方法，根据进行诊断的人员的熟练程度来减少结果分析的误差，以目标值显示各种免疫反应的程度，并进行各种 统计分析，因此对测量免疫反应非常有用。 板凝集试验仪由透明膜构成，其特征在于，在膜的上侧，在水平和垂直方向上布置并形成由多个圆组成并且发生抗原 - 抗体凝集反应的部分 。 测定免疫反应的方法包括以下步骤：（a）将抗原与抗原特异性结合的抗体与平板凝集试验仪混合以诱导凝集反应; （b）扫描凝集反应诱导的板凝集测试仪并将扫描结果转换为图像文件; 和（c）使用能够检测细微凝集反应物的计算机程序来分析所获得的图像文件，以对凝集反应的强度进行编号。 此外，透明膜是顶部投影膜。
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公开(公告)号：KR100787830B1
公开(公告)日：2008-01-02
申请号：KR1020060007213
申请日：2006-01-24
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명자는 돈단독불활화백신의 면역효능을 향상시키기 위하여 불활화한 돈단독균체에 돈단독균 특이방어항원인 분비단백질을 정제, 농축하여 첨가한 돈단독불활화백신의 면역능에 미치는 효과를 시험하여 다음과 같은 결과를 얻었다.
돈단독균 배양상층액중의 정제단백항원의 항체형성능이 균체항원보다 월등하였으며 마우스 방어효과도 보다 우수하였다. 또한 돈단독불활화 항원에 정제단백항원을 첨가하므로서 항체가 및 방어가 모두가 향상되었다.
상기의 불활화항원과 정제단백 혼합항원에 돈단독 특이항체 또는 glucan 등 면역증강물질을 첨가하므로서 항체가 및 방어효과의 현저한 상승효과를 나타내었다.
마우스에서 면역원성 향상효과가 확인된 항원으로 제조한 돈단독불활화백신을 목적동물인 이유자돈에 접종하고 항체형성능 및 안전성을 시험한 바 불활화항원만으로 만든 백신보다 균질화항원에 항체를 첨가하거나 불활화 항원에 정제단백 및 항체를 첨가한 군의 항체가가 월등히 높았으며 시험기간 동안 발열 등 이상반응이 관찰되지 않아 안전성이 확인되었다.
돈단독 불활화백신, 정제단백항원, 돈단독 특이항체, 면역증강물질-
8.发明公开돈단독균 불활화항원에 돈단독균 특이정제단백 항원을 첨가한 돈단독균 불활화백신의 제조방법 有权SWINE ERYSIPELAS杀死疫苗制备灭活细菌培养物和E.Husiopathiae的保护性蛋白抗原
公开(公告)号：KR1020070077588A
公开(公告)日：2007-07-27
申请号：KR1020060007213
申请日：2006-01-24
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K39/0241 , A61K2039/522 , A61K2039/552 , Y10S424/825
Abstract: A swine erysipelas inactivated vaccine is provided to significantly improve the immune capacity by adding a protein antigen obtained from a culture supernatant to a swine erysipelas inactivated antigen. The swine erysipelas inactivated vaccine is prepared by the method comprising the steps of: (a) after inoculating swine erysipelas seed into a swine erysipelas producing culture medium, culturing it at a temperature of 37 deg.C for 18-20 hours and adding formaline thereto to inactivate it, centrifuging it with the speed of 6,000 rpm for 30 minutes and collecting supernatant after removing settled down bacteria therefrom; (b) gradually adding ammonium sulfate to the supernatant until the solution being saturated to separate protein therefrom; (c) after putting the protein obtained from the step (b) in a dialysis membrane to remove impurities therefrom and obtain purified protein; (d) floating the purified protein in PBS(pH of 7.2) to prepare a swine erysipelas specific antigen; and (e) adding the swine erysipelas specific antigen to a swine erysipelas inactivated antigen to have 10-50% of the swine erysipelas specific antigen therein and mixing it. The swine erysipelas inactivated vaccine further comprises an immunostimulant.
Abstract translation: 提供猪丹毒灭活疫苗以通过将从培养上清获得的蛋白质抗原加入到猪丹毒灭活的抗原中来显着提高免疫能力。 通过包括以下步骤的方法制备猪丹毒灭活疫苗：（a）将猪丹毒种子接种到猪丹磺酸生产培养基中，在37℃的温度下培养18-20小时，并加入福尔马林 使其灭活，以6,000rpm的速度离心30分钟，并从其中除去沉降的细菌后收集上清液; （b）向上清液中逐渐加入硫酸铵，直到溶液饱和，从而分离蛋白质; （c）将从步骤（b）获得的蛋白质放入透析膜中以从其中除去杂质并获得纯化的蛋白质; （d）将纯化的蛋白质漂浮在PBS（pH 7.2）中以制备猪丹毒特异性抗原; 和（e）将猪丹毒特异性抗原加入到猪丹毒灭活的抗原中，使其中具有10-50％的猪丹毒特异性抗原并混合。 猪丹毒灭活疫苗还包含免疫刺激剂。
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公开(公告)号：KR100634585B1
公开(公告)日：2006-10-16
申请号：KR1020040042854
申请日：2004-06-11
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: G01N33/543
Abstract: 본 발명은 살모넬라 티피뮤리움(Salmonella typhimurium), 살모넬라 엠반다카(Salmonella mbandaka), 및 살모넬라 엔테라이티디스(Salmonella enteritidis)에서 추출한, 그룹특이성 및 민감성이 우수하고 항원성이 뛰어난 LPS(lipopolysaccharide)를 혼합하여 항원으로 사용하는 것을 특징으로 하는 동물의 살모넬라 감염항체 검사키트를 제공한다.
살모넬라 감염항체, 살모넬라 티피뮤리움, 살모넬라 엔테라이티디스, 살모넬라 엠반다카, 리포폴리사카라이드(LPS), ELISA, 검사키트-
公开(公告)号：KR1020060075483A
公开(公告)日：2006-07-04
申请号：KR1020040114282
申请日：2004-12-28
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: G01N33/53
Abstract: 본 발명은 소 타일레리아병을 진단하는 방법에 관한 것으로서, 보다 상세하게는 라텍스 비드에 코팅된 재조합 p33 단백질에 소의 혈청을 반응시켜 침전여부를 확인함으로써 소 타일레리아병을 진단하는 방법에 관한 것이다. 본 발명의 진단 방법은 소 타일레리아병에 대한 높은 민감도와 특이도를 나타내며, 별도의 장비가 필요없이 육안으로도 결과를 판정할 수 있기 때문에 야외에서 편리하게 소 타일레리아병을 진단하는데 유용하게 사용될 수 있다.
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