公开(公告)号：KR1020120069446A

公开(公告)日：2012-06-28

申请号：KR1020100131000

申请日：2010-12-20

Applicant: 대한민국(농촌진흥청장)

Inventor： 조강희 ,  신일섭 ,  김정희 ,  김세희 ,  김대현 ,  허성 ,  김현란

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12Q1/6895 ,  C12Q2535/138 ,  C12Q2535/139 ,  C12Q2600/13

Abstract: PURPOSE: A SCAR marker for distinguishing pear species is provided to accurately distinguish native species and introduced species. CONSTITUTION: A SCAR marker for distinguishing pear species contains: P123_279 primer set of an oligonucleotide of sequence numbers 1 and 2; P561_331 primer set of an oligonucleotide of sequence numbers 3 and 4; P561_372 primer set having an oligonucleotide of sequence numbers 5 and 6; PH02_405 primer set having an oligonucleotide of sequence numbers 7 and 8; PK15_410 primer set having an oligonucleotide of sequence numbers 9 and 10; PH15_452 primer set having an oligonucleotide of sequence numbers 11 and 12; PT16_472 primer set having an oligonucleotide of sequence numbers 13 and 14; PT06_509 prime set having an oligonucleotide of sequence numbers of sequence numbers 15 and 16; PN11_516 primer set having an oligonucleotide of sequence numbers 17 and 18; PK17_556 primer set having an oligonucleotide of sequence numbers 19 and 20; PN15_575 primer set having an oligonucleotide of sequence numbers 21 and 22; PT14_578 primer set having an oligonucleotide of sequence numbers 23 and 24; P270_593 primer set having an oligonucleotide of sequence numbers 25 and 26; PF07_594 primer set having an oligonucleotide of sequence numbers 27 and 28; PQ11_649 primer set having an oligonucleotide of sequence numbers 29 and 30; PK15_672 primer set having an oligonucleotide of sequence numbers 31 and 32; PK17_771 primer set having an oligonucleotide of sequence numbers 33 and 34; PK10_514 primer set having an oligonucleotide of sequence numbers 35 and 36; or PK15_616 primer set having an oligonucleotide of sequence numbers 37 and 38.

Abstract translation: 目的：提供用于区分梨种的SCAR标记，以准确区分本地物种和引种物种。 构成：用于区分梨物种的SCAR标记物包含：序列号1和2的寡核苷酸的引物组：P123_279; P561_331序列号3和4的寡核苷酸的引物组; P561_372具有序列号5和6的寡核苷酸的引物组; 具有序列号7和8的寡核苷酸的PH02_405引物组; 具有序列号9和10的寡核苷酸的PK15_410引物组; 具有序列号11和12的寡核苷酸的PH15_452引物组; 具有序列号13和14的寡核苷酸的PT16_472引物组; PT06_509具有序列号为15和16的序列号的寡核苷酸的序列; 具有序列号17和18的寡核苷酸的PN11_516引物组; 具有序列号19和20的寡核苷酸的PK17_556引物组; 具有序列号21和22的寡核苷酸的PN15_575引物组; 具有序列号23和24的寡核苷酸的PT14_578引物组; P270_593具有序列号25和26的寡核苷酸的引物组; 具有序列号27和28的寡核苷酸的PF07_594引物组; PQ11_649具有序列号29和30的寡核苷酸的引物组; 具有序列号31和32的寡核苷酸的PK15_672引物组; 具有序列号33和34的寡核苷酸的PK17_771引物组; 具有序列号35和36的寡核苷酸的PK10_514引物组; 或具有序列号37和38的寡核苷酸的PK15_616引物组。