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公开(公告)号：KR200461955Y1
公开(公告)日：2012-08-22
申请号：KR2020090001646
申请日：2009-02-13
Applicant: 대한민국(농촌진흥청장)
IPC: A01H1/02
Abstract: 화분을 채취하도록 가위형태로 마련된 채취기와, 채취기에 의해 채취된 화분을 바로 수집하도록 마련된 수집통을 일체로 구비하는 화분채취기구가 개시된다.
이와 같이 마련되는 화분채취기구는 화분을 거의 채취와 동시에 수집할 수 있게 되어 화분채취작업성을 향상시키게 되며, 화분 채취를 위해 꽃을 크게 손상시킬 우려가 없어 화분을 자가채집하기에 적합하다.Abstract translation: 具有布置和剪刀的形式提供，以收集花粉集电极，以便立即收集由收集器收集的花粉一体地设置的收集器的收集花粉机制。
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公开(公告)号：KR1020120069446A
公开(公告)日：2012-06-28
申请号：KR1020100131000
申请日：2010-12-20
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6895 , C12Q2535/138 , C12Q2535/139 , C12Q2600/13
Abstract: PURPOSE: A SCAR marker for distinguishing pear species is provided to accurately distinguish native species and introduced species. CONSTITUTION: A SCAR marker for distinguishing pear species contains: P123_279 primer set of an oligonucleotide of sequence numbers 1 and 2; P561_331 primer set of an oligonucleotide of sequence numbers 3 and 4; P561_372 primer set having an oligonucleotide of sequence numbers 5 and 6; PH02_405 primer set having an oligonucleotide of sequence numbers 7 and 8; PK15_410 primer set having an oligonucleotide of sequence numbers 9 and 10; PH15_452 primer set having an oligonucleotide of sequence numbers 11 and 12; PT16_472 primer set having an oligonucleotide of sequence numbers 13 and 14; PT06_509 prime set having an oligonucleotide of sequence numbers of sequence numbers 15 and 16; PN11_516 primer set having an oligonucleotide of sequence numbers 17 and 18; PK17_556 primer set having an oligonucleotide of sequence numbers 19 and 20; PN15_575 primer set having an oligonucleotide of sequence numbers 21 and 22; PT14_578 primer set having an oligonucleotide of sequence numbers 23 and 24; P270_593 primer set having an oligonucleotide of sequence numbers 25 and 26; PF07_594 primer set having an oligonucleotide of sequence numbers 27 and 28; PQ11_649 primer set having an oligonucleotide of sequence numbers 29 and 30; PK15_672 primer set having an oligonucleotide of sequence numbers 31 and 32; PK17_771 primer set having an oligonucleotide of sequence numbers 33 and 34; PK10_514 primer set having an oligonucleotide of sequence numbers 35 and 36; or PK15_616 primer set having an oligonucleotide of sequence numbers 37 and 38.
Abstract translation: 目的：提供用于区分梨种的SCAR标记，以准确区分本地物种和引种物种。 构成：用于区分梨物种的SCAR标记物包含：序列号1和2的寡核苷酸的引物组：P123_279; P561_331序列号3和4的寡核苷酸的引物组; P561_372具有序列号5和6的寡核苷酸的引物组; 具有序列号7和8的寡核苷酸的PH02_405引物组; 具有序列号9和10的寡核苷酸的PK15_410引物组; 具有序列号11和12的寡核苷酸的PH15_452引物组; 具有序列号13和14的寡核苷酸的PT16_472引物组; PT06_509具有序列号为15和16的序列号的寡核苷酸的序列; 具有序列号17和18的寡核苷酸的PN11_516引物组; 具有序列号19和20的寡核苷酸的PK17_556引物组; 具有序列号21和22的寡核苷酸的PN15_575引物组; 具有序列号23和24的寡核苷酸的PT14_578引物组; P270_593具有序列号25和26的寡核苷酸的引物组; 具有序列号27和28的寡核苷酸的PF07_594引物组; PQ11_649具有序列号29和30的寡核苷酸的引物组; 具有序列号31和32的寡核苷酸的PK15_672引物组; 具有序列号33和34的寡核苷酸的PK17_771引物组; 具有序列号35和36的寡核苷酸的PK10_514引物组; 或具有序列号37和38的寡核苷酸的PK15_616引物组。
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公开(公告)号：KR101144988B1
公开(公告)日：2012-06-27
申请号：KR1020090131107
申请日：2009-12-24
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A SCAR(sequence characterized amplified region) marker for determining apples is provided to reinforce protection of apple species and quickly and accurately certify apple species. CONSTITUTION: A SCAR marker for determining apple species contains AL1_343(sequence number 35[SEQ ID NO:35]), AW15_368(sequence number 36), A330_424(sequence number 37), AL1_427(sequence number 38), AN11_433(sequence number 39), AT6_453 (sequence number 40), AG14_502 (sequence number 41), AN8_566 (sequence number 42), AK17_574 (sequence number 43), AK14_575 (sequence number 44), A408_592 (sequence number 45), AK17_653 (sequence number 46), AM16_708 (sequence number 47), AO4_711 (sequence number 48), AO4_779 (sequence number 49), AT14_789 (sequence number 50), or AL1_851(sequence number 51). A kit for determining apple species comprises: oligonucleotide SCAR primer set for amplifying SCAR marker; and reagent for amplification.
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公开(公告)号：KR1020120067473A
公开(公告)日：2012-06-26
申请号：KR1020100128885
申请日：2010-12-16
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6851 , C12Q1/6895 , C12Q1/70 , G01N33/5097
Abstract: PURPOSE: A primer for diagnosing apple mosaic virus(ApMV) infection using SYBR Green I is provided to accurately diagnose the infection and to enhance detection efficiency. CONSTITUTION: A primer set for diagnosing apple mosaic virus(ApMV) infection contains a primer of sequence number 1 and a primer of sequence number 2. A method for real-time detection method for ApMV comprises: a step of performing real-time PCR using sample DNA or partial DNA using a marker and primer set; and a step of analyzing curve of the real-time PCR products. The marker is SYBR green I.
Abstract translation: 目的：提供用于诊断使用SYBR Green I的苹果花叶病毒（ApMV）感染的引物，以准确诊断感染并提高检测效率。 构成：用于诊断苹果花叶病毒（ApMV）感染的引物组包含序列号1的引物和序列号为2的引物。用于ApMV的实时检测方法的方法包括：使用 使用标记物和引物组对样本DNA或部分DNA进行检测; 以及分析实时PCR产物曲线的步骤。 标记是SYBR绿I。
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公开(公告)号：KR101258694B1
公开(公告)日：2013-04-29
申请号：KR1020100131000
申请日：2010-12-20
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 서열번호 1 내지 38의 올리고뉴클레오티드로 구성된 프라이머 세트의 군으로부터 선택되는 8 이상의 프라이머 세트를 포함하는 배 품종 식별용 SCAR 프라이머 세트 조성물 및 그 용도에 관한 것이다.
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Patent Agency Ranking
公开(公告)号：KR1020120067481A
公开(公告)日：2012-06-26
申请号：KR1020100128900
申请日：2010-12-16
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6851 , C12Q1/6895 , C12Q1/70 , C12Q2563/107 , G01N33/5097
Abstract: PURPOSE: A primer for diagnosing apple stem pitting virus using SYBR green I is provided to accurately diagnose the infection and to enhance diagnosis efficiency. CONSTITUTION: A primer set for diagnosing ASPV(apple stem pitting virus) infection contain primers of sequence numbers 1 and 2. A method for real-time detection of the infection comprises: a step of performing real-time PCR of DNA from a subject and a part of the DNA using the primer set and a marker; and a step of analyzing curve of the real-time PCR product. The marker is SYBR Green I.
Abstract translation: 目的：使用SYBR绿I诊断苹果茎点蚀病毒的底漆，以准确诊断感染并提高诊断效率。 构成：用于诊断ASPV（苹果干点蚀病毒）感染的引物组含有序列号1和2的引物。用于实时检测感染的方法包括：从受试者进行DNA的实时PCR的步骤， 使用引物组和标记物的DNA的一部分; 以及分析实时PCR产物曲线的步骤。 标记是SYBR Green I.
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公开(公告)号：KR1020100010349A
公开(公告)日：2010-02-01
申请号：KR1020080071277
申请日：2008-07-22
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q2600/112 , C12Q2600/13
Abstract: PURPOSE: A primer for diagnosing ASSVd(apple scar skin viroid) infection and a diagnosis method using the same are provided to accurately detect infection by ASSVd and improve diagnosis accuracy. CONSTITUTION: A primer for diagnosing ASSVd(apple scar skin viroid) comprises a sequence number 1 or 2. A method of NASBA(nucleic acid sequence based amplification) for diagnosing infection by ASSVd comprises: a first step of annealing a first primer on a sense RNA and synthesizing single-stranded DNA molecule; a second step of reacting RNase to DNA-RNA hybrid molecule to remove RNA; a third step of annealing a second primer on single-stranded DNA molecule and reacting reverse transcriptase to synthesize double stranded DNA; and a step of synthesizing antisense RNA mediating RNA polymerase with single-stranded DNA molecule as an template.
Abstract translation: 目的：提供用于诊断ASSVd（苹果瘢痕皮肤病毒）感染的引物和使用其的诊断方法，以准确检测ASSVd感染并提高诊断准确性。 构成：用于诊断ASSVd（苹果瘢痕皮肤病毒）的引物包含序列号1或2.一种用于诊断ASSVd感染的NASBA（基于核酸序列的扩增）的方法包括：第一步骤，对第一引物进行退火 RNA和合成单链DNA分子; 使RNA酶与DNA-RNA杂交分子反应以除去RNA的第二步; 将第二引物退火在单链DNA分子上并使反转录酶反应合成双链DNA的第三步骤; 以单链DNA分子为模板合成反义RNA介导RNA聚合酶的步骤。
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公开(公告)号：KR1020110074204A
公开(公告)日：2011-06-30
申请号：KR1020090131107
申请日：2009-12-24
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6844 , C12Q2600/158
Abstract: PURPOSE: A SCAR(sequence characterized amplified region) marker for determining apples is provided to reinforce protection of apple species and quickly and accurately certify apple species. CONSTITUTION: A SCAR marker for determining apple species contains AL1_343(sequence number 35[SEQ ID NO:35]), AW15_368(sequence number 36), A330_424(sequence number 37), AL1_427(sequence number 38), AN11_433(sequence number 39), AT6_453 (sequence number 40), AG14_502 (sequence number 41), AN8_566 (sequence number 42), AK17_574 (sequence number 43), AK14_575 (sequence number 44), A408_592 (sequence number 45), AK17_653 (sequence number 46), AM16_708 (sequence number 47), AO4_711 (sequence number 48), AO4_779 (sequence number 49), AT14_789 (sequence number 50), or AL1_851(sequence number 51). A kit for determining apple species comprises: oligonucleotide SCAR primer set for amplifying SCAR marker; and reagent for amplification.
Abstract translation: 目的：提供确定苹果的SCAR（序列特征扩增区）标记，以加强对苹果物种的保护，并快速，准确地认证苹果种类。 构成：用于确定苹果物种的SCAR标记包含AL1_343（序列号35 [SEQ ID NO：35]），AW15_368（序列号36），A330_424（序列号37），AL1_427（序列号38），AN11_433（序列号39 ），AT6_453（序列号40），AG14_502（序列号41），AN8_566（序列号42），AK17_574（序列号43），AK14_575（序列号44），A408_592（序列号45），AK17_653（序列号46） ，AM16_708（序列号47），AO4_711（序列号48），AO4_779（序列号49），AT14_789（序列号50）或AL1_851（序列号51）。 用于测定苹果种的试剂盒包括：用于扩增SCAR标记的寡核苷酸SCAR引物组; 和扩增试剂。
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公开(公告)号：KR101024493B1
公开(公告)日：2011-03-24
申请号：KR1020080071277
申请日：2008-07-22
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 일측에 RNA 폴리머라제 프라이머가 결합되는 서열번호 1로 이루어지는 프라이머를 제 1프라이머로 하여 NASBA 검정법에 의해 사과 바이로이드 안티센스 RNA를 증폭하여 사과 바이로이드 감염여부를 확인하는 사과 바이로이드 진단방법을 제공한다.
ASSVd, NASBA-
公开(公告)号：KR2020100008377U
公开(公告)日：2010-08-23
申请号：KR2020090001646
申请日：2009-02-13
Applicant: 대한민국(농촌진흥청장)
IPC: A01H1/02
Abstract: 화분을 채취하도록 가위형태로 마련된 채취기와, 채취기에 의해 채취된 화분을 바로 수집하도록 마련된 수집통을 일체로 구비하는 화분채취기구가 개시된다.
이와 같이 마련되는 화분채취기구는 화분을 거의 채취와 동시에 수집할 수 있게 되어 화분채취작업성을 향상시키게 되며, 화분 채취를 위해 꽃을 크게 손상시킬 우려가 없어 화분을 자가채집하기에 적합하다.
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