Abstract:
PURPOSE: A SCAR(sequence characterized amplified region) marker for determining apples is provided to reinforce protection of apple species and quickly and accurately certify apple species. CONSTITUTION: A SCAR marker for determining apple species contains AL1_343(sequence number 35[SEQ ID NO:35]), AW15_368(sequence number 36), A330_424(sequence number 37), AL1_427(sequence number 38), AN11_433(sequence number 39), AT6_453 (sequence number 40), AG14_502 (sequence number 41), AN8_566 (sequence number 42), AK17_574 (sequence number 43), AK14_575 (sequence number 44), A408_592 (sequence number 45), AK17_653 (sequence number 46), AM16_708 (sequence number 47), AO4_711 (sequence number 48), AO4_779 (sequence number 49), AT14_789 (sequence number 50), or AL1_851(sequence number 51). A kit for determining apple species comprises: oligonucleotide SCAR primer set for amplifying SCAR marker; and reagent for amplification.
Abstract translation:目的:提供确定苹果的SCAR(序列特征扩增区)标记,以加强对苹果物种的保护,并快速,准确地认证苹果种类。 构成:用于确定苹果物种的SCAR标记包含AL1_343(序列号35 [SEQ ID NO:35]),AW15_368(序列号36),A330_424(序列号37),AL1_427(序列号38),AN11_433(序列号39 ),AT6_453(序列号40),AG14_502(序列号41),AN8_566(序列号42),AK17_574(序列号43),AK14_575(序列号44),A408_592(序列号45),AK17_653(序列号46) ,AM16_708(序列号47),AO4_711(序列号48),AO4_779(序列号49),AT14_789(序列号50)或AL1_851(序列号51)。 用于测定苹果种的试剂盒包括:用于扩增SCAR标记的寡核苷酸SCAR引物组; 和扩增试剂。
Abstract:
본 발명은 일측에 RNA 폴리머라제 프라이머가 결합되는 서열번호 1로 이루어지는 프라이머를 제 1프라이머로 하여 NASBA 검정법에 의해 사과 바이로이드 안티센스 RNA를 증폭하여 사과 바이로이드 감염여부를 확인하는 사과 바이로이드 진단방법을 제공한다. ASSVd, NASBA
Abstract:
PURPOSE: A PCR test system for diagnosing GFLV(grapevine fanleaf virus) is provided to diagnose and to easily diagnose. CONSTITUTION: A primer for diagnosing grapevine fanleaf virus is primer complex sequence numbers 8 and 19, sequence numbers 9 and 18, sequence number 11 and 16, and sequence number 12 and 15. The diagnosis is performed by RT-PCR using the primer with a genome RNA as a template.
Abstract:
본 발병은 포도 등에 발생하고 있는 포도부채잎바이러스(GFLV)를 진단하기 위한 PCR 검사시스템에 관한 것이다. PCR 검사시스템은 GFLV 진단용 최적 프라이머 조합 및 이에 부합하는 검정용 프라이머 조합(nested primer)과 이를 뒷받침할 수 있는 종 특이 프라이머 조합 및 양성대조구를 포함하고 있다.
Abstract:
PURPOSE: A primer for diagnosing apple stem pitting virus using SYBR green I is provided to accurately diagnose the infection and to enhance diagnosis efficiency. CONSTITUTION: A primer set for diagnosing ASPV(apple stem pitting virus) infection contain primers of sequence numbers 1 and 2. A method for real-time detection of the infection comprises: a step of performing real-time PCR of DNA from a subject and a part of the DNA using the primer set and a marker; and a step of analyzing curve of the real-time PCR product. The marker is SYBR Green I.
Abstract translation:目的:使用SYBR绿I诊断苹果茎点蚀病毒的底漆,以准确诊断感染并提高诊断效率。 构成:用于诊断ASPV(苹果干点蚀病毒)感染的引物组含有序列号1和2的引物。用于实时检测感染的方法包括:从受试者进行DNA的实时PCR的步骤, 使用引物组和标记物的DNA的一部分; 以及分析实时PCR产物曲线的步骤。 标记是SYBR Green I.
Abstract:
PURPOSE: A primer for diagnosing ASSVd(apple scar skin viroid) infection and a diagnosis method using the same are provided to accurately detect infection by ASSVd and improve diagnosis accuracy. CONSTITUTION: A primer for diagnosing ASSVd(apple scar skin viroid) comprises a sequence number 1 or 2. A method of NASBA(nucleic acid sequence based amplification) for diagnosing infection by ASSVd comprises: a first step of annealing a first primer on a sense RNA and synthesizing single-stranded DNA molecule; a second step of reacting RNase to DNA-RNA hybrid molecule to remove RNA; a third step of annealing a second primer on single-stranded DNA molecule and reacting reverse transcriptase to synthesize double stranded DNA; and a step of synthesizing antisense RNA mediating RNA polymerase with single-stranded DNA molecule as an template.
Abstract:
The present invention relates to a sequence characterized amplified region (SCAR) marker for identification of Persimmon cultivars and uses thereof, and more specifically to a SCAR marker for identification of persimmon cultivars, a kit for identification of persimmon cultivars comprising a SCAR primer set, and a method for determining the variety of Persimmon cultivars using the SCAR primer set.