사과의 품종 판별용 SCAR 표지 및 이의 용도
    1.
    发明公开
    사과의 품종 판별용 SCAR 표지 및 이의 용도 有权
    用于歧视苹果品种及其用途的标识标记

    公开(公告)号:KR1020110074204A

    公开(公告)日:2011-06-30

    申请号:KR1020090131107

    申请日:2009-12-24

    CPC classification number: C12N15/11 C12Q1/6844 C12Q2600/158

    Abstract: PURPOSE: A SCAR(sequence characterized amplified region) marker for determining apples is provided to reinforce protection of apple species and quickly and accurately certify apple species. CONSTITUTION: A SCAR marker for determining apple species contains AL1_343(sequence number 35[SEQ ID NO:35]), AW15_368(sequence number 36), A330_424(sequence number 37), AL1_427(sequence number 38), AN11_433(sequence number 39), AT6_453 (sequence number 40), AG14_502 (sequence number 41), AN8_566 (sequence number 42), AK17_574 (sequence number 43), AK14_575 (sequence number 44), A408_592 (sequence number 45), AK17_653 (sequence number 46), AM16_708 (sequence number 47), AO4_711 (sequence number 48), AO4_779 (sequence number 49), AT14_789 (sequence number 50), or AL1_851(sequence number 51). A kit for determining apple species comprises: oligonucleotide SCAR primer set for amplifying SCAR marker; and reagent for amplification.

    Abstract translation: 目的:提供确定苹果的SCAR(序列特征扩增区)标记,以加强对苹果物种的保护,并快速,准确地认证苹果种类。 构成:用于确定苹果物种的SCAR标记包含AL1_343(序列号35 [SEQ ID NO:35]),AW15_368(序列号36),A330_424(序列号37),AL1_427(序列号38),AN11_433(序列号39 ),AT6_453(序列号40),AG14_502(序列号41),AN8_566(序列号42),AK17_574(序列号43),AK14_575(序列号44),A408_592(序列号45),AK17_653(序列号46) ,AM16_708(序列号47),AO4_711(序列号48),AO4_779(序列号49),AT14_789(序列号50)或AL1_851(序列号51)。 用于测定苹果种的试剂盒包括:用于扩增SCAR标记的寡核苷酸SCAR引物组; 和扩增试剂。

    포도부채잎바이러스 피시알 검사 시스템
    3.
    发明公开
    포도부채잎바이러스 피시알 검사 시스템 有权
    PCR检测系统,用于格拉菲犬FANLEAF VIRUSSPOT病毒

    公开(公告)号:KR1020100083607A

    公开(公告)日:2010-07-22

    申请号:KR1020090003073

    申请日:2009-01-14

    CPC classification number: C12Q1/70 C12N15/11 C12Q1/686 G01N33/56983

    Abstract: PURPOSE: A PCR test system for diagnosing GFLV(grapevine fanleaf virus) is provided to diagnose and to easily diagnose. CONSTITUTION: A primer for diagnosing grapevine fanleaf virus is primer complex sequence numbers 8 and 19, sequence numbers 9 and 18, sequence number 11 and 16, and sequence number 12 and 15. The diagnosis is performed by RT-PCR using the primer with a genome RNA as a template.

    Abstract translation: 目的:提供诊断GFLV(葡萄叶扇叶病毒)的PCR检测系统进行诊断和诊断。 构成:用于诊断葡萄扇叶病毒的引物是引物复合序列号8和19,序列号9和18,序列号11和16以及序列号12和15.通过RT-PCR进行诊断,使用引物 基因组RNA作为模板。

    국내 분리주 사과줄기패임바이러스 감염 진단을 위한 프라이머 및 이를 이용한 실시간 검정법
    6.
    发明公开
    국내 분리주 사과줄기패임바이러스 감염 진단을 위한 프라이머 및 이를 이용한 실시간 검정법 无效
    用于诊断苹果病毒感染(ASPV)感染的方法和使用实时PCR诊断方法

    公开(公告)号:KR1020120067481A

    公开(公告)日:2012-06-26

    申请号:KR1020100128900

    申请日:2010-12-16

    Abstract: PURPOSE: A primer for diagnosing apple stem pitting virus using SYBR green I is provided to accurately diagnose the infection and to enhance diagnosis efficiency. CONSTITUTION: A primer set for diagnosing ASPV(apple stem pitting virus) infection contain primers of sequence numbers 1 and 2. A method for real-time detection of the infection comprises: a step of performing real-time PCR of DNA from a subject and a part of the DNA using the primer set and a marker; and a step of analyzing curve of the real-time PCR product. The marker is SYBR Green I.

    Abstract translation: 目的:使用SYBR绿I诊断苹果茎点蚀病毒的底漆,以准确诊断感染并提高诊断效率。 构成:用于诊断ASPV(苹果干点蚀病毒)感染的引物组含有序列号1和2的引物。用于实时检测感染的方法包括:从受试者进行DNA的实时PCR的步骤, 使用引物组和标记物的DNA的一部分; 以及分析实时PCR产物曲线的步骤。 标记是SYBR Green I.

    사과 바이로이드 감염 진단을 위한 프라이머 및 이를이용한 진단방법
    7.
    发明公开
    사과 바이로이드 감염 진단을 위한 프라이머 및 이를이용한 진단방법 有权
    用于诊断苹果皮肤病毒感染的准备和使用该方法进行诊断的方法

    公开(公告)号:KR1020100010349A

    公开(公告)日:2010-02-01

    申请号:KR1020080071277

    申请日:2008-07-22

    CPC classification number: C12Q1/701 C12Q2600/112 C12Q2600/13

    Abstract: PURPOSE: A primer for diagnosing ASSVd(apple scar skin viroid) infection and a diagnosis method using the same are provided to accurately detect infection by ASSVd and improve diagnosis accuracy. CONSTITUTION: A primer for diagnosing ASSVd(apple scar skin viroid) comprises a sequence number 1 or 2. A method of NASBA(nucleic acid sequence based amplification) for diagnosing infection by ASSVd comprises: a first step of annealing a first primer on a sense RNA and synthesizing single-stranded DNA molecule; a second step of reacting RNase to DNA-RNA hybrid molecule to remove RNA; a third step of annealing a second primer on single-stranded DNA molecule and reacting reverse transcriptase to synthesize double stranded DNA; and a step of synthesizing antisense RNA mediating RNA polymerase with single-stranded DNA molecule as an template.

    Abstract translation: 目的:提供用于诊断ASSVd(苹果瘢痕皮肤病毒)感染的引物和使用其的诊断方法,以准确检测ASSVd感染并提高诊断准确性。 构成:用于诊断ASSVd(苹果瘢痕皮肤病毒)的引物包含序列号1或2.一种用于诊断ASSVd感染的NASBA(基于核酸序列的扩增)的方法包括:第一步骤,对第一引物进行退火 RNA和合成单链DNA分子; 使RNA酶与DNA-RNA杂交分子反应以除去RNA的第二步; 将第二引物退火在单链DNA分子上并使反转录酶反应合成双链DNA的第三步骤; 以单链DNA分子为模板合成反义RNA介导RNA聚合酶的步骤。

    신고 배 조직 배양용 배지
    8.
    发明公开
    신고 배 조직 배양용 배지 有权
    NIITAKA PEARS组织培养基

    公开(公告)号:KR1020160042503A

    公开(公告)日:2016-04-20

    申请号:KR1020140136456

    申请日:2014-10-10

    CPC classification number: C12N5/0025 A01H4/00 C12N5/00

    Abstract: 본발명은신고배의조직배양용배지에관한것으로서, 더욱상세하게는 MS 무기염, LS비타민, NAA (1-Naphthaleneacetic acid), BA (benzylaminopurine), 탄소공급원(carbon source), 겔화제(gelling agent) 및증류수를포함함으로써신고배의신초재분화율을향상시킬수 있는식물조직배양용배지에관한것이다. 본발명에따른신고배 조직배양용배지를사용함으로써신고배의재분화율을 16.4%까지높일수 있는신고배 재분화체계를구축할수 있다.

    Abstract translation: 本发明涉及Niitaka梨的组织培养培养基,更具体地涉及用于植物组织培养的培养基,其可以通过包括MS无机盐,LS维生素,1-萘乙酸来提高日本梨的芽再分化速率 (NAA),苄基氨基嘌呤(BA),碳源,胶凝剂和蒸馏水。 通过使用Niitaka梨的组织培养培养基构建新泻梨的再分化系统,其中Niitaka梨的再分化速率可以提高至16.4%。

    감의 품종 판별용 스카 마커 조성물 및 이의 용도
    9.
    发明公开
    감의 품종 판별용 스카 마커 조성물 및 이의 용도 有权
    用于识别PERSIMMON CULTIVARS的标记标记及其用途

    公开(公告)号:KR1020140065617A

    公开(公告)日:2014-05-30

    申请号:KR1020120130687

    申请日:2012-11-19

    CPC classification number: C12Q1/6844 C12N15/11

    Abstract: The present invention relates to a sequence characterized amplified region (SCAR) marker for identification of Persimmon cultivars and uses thereof, and more specifically to a SCAR marker for identification of persimmon cultivars, a kit for identification of persimmon cultivars comprising a SCAR primer set, and a method for determining the variety of Persimmon cultivars using the SCAR primer set.

    Abstract translation: 本发明涉及用于鉴定柿子品种的扩增区(SCAR)标记的序列及其用途,更具体地涉及用于鉴定柿子品种的SCAR标记物,用于鉴定包含SCAR引物组的柿子品种的试剂盒,以及 使用SCAR引物组确定柿子品种的方法。

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