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公开(公告)号：KR102081586B1
公开(公告)日：2020-02-26
申请号：KR1020180159456
申请日：2018-12-11
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/6895 , C12Q1/6851
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公开(公告)号：KR101761293B1
公开(公告)日：2017-07-25
申请号：KR1020150113901
申请日：2015-08-12
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: 본발명은무 위황병저항성판별용프라이머세트및 이를포함하는키트에관한것으로, 구체적으로본 발명을통해무 위황병저항성과관련되는것으로밝혀진단일염기다형성(single nucleotide polymorphism, SNP)에따른유전자형을판별함으로써유전적으로위황병저항성이높거나낮은무를효과적으로판별해낼수 있도록하는프라이머세트, 조성물및 키트에관한것이다. 본발명에따르면무의위황병에대한저항성을빠르고정확하게판별할수 있다. 이에따라위황병에대한저항성이우수한무를선별하여재배할수 있어위황병으로인한농가의피해를줄이는데크게기여할수 있을뿐만아니라위황병에대한저항성을갖으면서다른유용한특성을갖는새로운품종을개발하는데에도매우유용할것이라기대된다.
Abstract translation: 本发明没有涉及电阻萎黄测定引物和试剂盒通过确定特定SNP（单核苷酸多态性，SNP）的基因型包括用于，油相同的已经发现通过实施本发明来影响没有萎黄电阻 本发明涉及一种用于有效区分对抗耐硼砂性的高或低耐受性的引物组，组合物和试剂盒。 根据本发明，可以快速和准确地鉴别对萝卜应变的抗性。 Yiettara而它可以长到到萎黄筛选萝卜性优异，不仅可以大大有助于减少农民的影响。由于萎黄gateu到萎黄预计将在发展与不同的实用功能的新品种有用性 是的。
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公开(公告)号：KR1020160042506A
公开(公告)日：2016-04-20
申请号：KR1020140136464
申请日：2014-10-10
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/02
Abstract: 본발명은종자를파종하여발생한본엽을잘라내는단계; 상기잘라낸본엽을챔버에넣고균주현탁액을분무하는단계; 및상기균주현탁액을분무한상기잘라낸본엽을통해서수박의병저항성을검정하는단계;를포함하는수박병저항성검정방법에관한것이다.
Abstract translation: 本发明涉及一种西瓜抗病检测方法，包括以下步骤：切割种子播种的真叶; 将切割的真叶插入室中并喷洒应变悬浮液; 并通过用应变悬浮液喷洒的切割真叶检查西瓜的抗病性。 该方法能够减少检查时间。
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14.发明授权
公开(公告)号：KR101522600B1
公开(公告)日：2015-05-26
申请号：KR1020130035303
申请日：2013-04-01
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명의목적은배추병저항성연관 SNP 프라이머쌍 7,645개의활용성을확대하고자, 배추의뿌리혹병및 모자이크바이러스병저항성과잎 특성을동시에평가할수 있는 SNP 분자마커를개발하고, 이의용도를제공하는것이다.
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Patent Agency Ranking
公开(公告)号：KR1020110093164A
公开(公告)日：2011-08-18
申请号：KR1020100013042
申请日：2010-02-11
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/8205 , A01H4/001 , A01H4/005 , C12N5/0025 , C12N15/8216 , C12N15/8241
Abstract: PURPOSE: A method for enhancing transformation efficiency of Brassica campestris L. using Agrobacterium is provided to establish stable transformation system of high efficiency. CONSTITUTION: A method for enhancing transformation efficiency of Brassica campestris L. comprises: a step of performing dark germination of Brassica campestris L. seeds at 24-26°C for 3-5 days; a step of culturing the hypocotyl part of Brassica campestris L. in a MS medium containing 0.5-1.5 mg/L of NAA and 3-5 mg/L of BA at 22-24°C for 1-3 days by photo-cultivation; a step of suspending Agrobacterium tumefaciens containing a recombinanat plant expression vector to a medium; a step of dipping the hypocotyl part of Brassica campestris L. in the prepared strain-inoculated medium for 5-15 minutes; a step of culturing the hypocotyl part of Brassica campestris L. in a medium I containing NAA, BA, AgNO_3, and antibiotics for 2-3 months to induce a shoot; and a step of culturing the shoot in a medium II.
Abstract translation: 目的：提供使用农杆菌提高芥菜转化效率的方法，建立高效率的稳定转化体系。 构成：提高芥菜转化效率的方法包括：在24-26℃下进行芸苔属种子黑萌发3-5天的步骤; 在含有0.5-1.5mg / L的NAA和3-5mg / L BA的MS培养基中培养芥菜的下胚轴部分在22-24℃下培养1-3天的步骤; 将含有重组酶植物表达载体的根癌农杆菌悬浮于培养基中的步骤; 将芥菜的下胚轴部分浸渍在制备的菌株接种培养基中5-15分钟的步骤; 在含有NAA，BA，AgNO_3和抗生素的培养基I中培养芥菜的下胚轴2-3个月以诱导芽的步骤; 并在培养基II培养苗条的步骤。
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公开(公告)号：KR1020110042400A
公开(公告)日：2011-04-27
申请号：KR1020090099059
申请日：2009-10-19
Applicant: 대한민국(농촌진흥청장) , 서울대학교산학협력단
CPC classification number: C12N15/8283 , C12N15/8213 , C12N15/8225 , C12Q1/6895
Abstract: PURPOSE: A method for growing Brassica campestris L. with turnip mosaic virus resistance is provided to stably and effectively select and grow TuMV resistance. CONSTITUTION: A Brassica campestris L. with turnip mosaic virus resistance is prepared by mutation of Brassica campestris L.-derived eIF(iso)4E 49th, 95th, 150th, or 191th amino acid. A 49th, 95th, 150th, or 191th amino acid is substituted with leucine.
Abstract translation: 目的：提供芜菁花叶病毒耐药性栽培芸苔属植物的方法，以稳定有效地选择和生长TuMV抗性。 构成：具有芜菁花叶病毒抗性的芸苔属芥菜L.通过突变芸苔属油菜来源的eIF（iso）4E第49,95，150或191位氨基酸制备。 第49，第95，第150或第191位氨基酸被亮氨酸取代。
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公开(公告)号：KR100948980B1
公开(公告)日：2010-03-25
申请号：KR1020070124615
申请日：2007-12-03
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 아그로박테리움을 이용한 마늘의 형질전환 효율을 증가시키는 방법 및 상기 방법에 의해 제조된 마늘 식물체에 관한 것으로, 더욱 상세하게는 (1) MS 배지에
0.5~2 mg/L의 2,4-D(2,4-dichlorophenoxyacetic acid)와 0.05~0.2mg/L의 IAA(indoleacetic acid)를 첨가하여 7~9주간 마늘 캘러스를 유도하는 단계; (2) 상기 유도된 마늘 캘러스에 재조합 식물 발현 벡터를 포함하는 아그로박테리움 튜머파시엔스
(Agrobacterium tumefaciens) 를 공동배양 배지에서 공동배양하여 형질전환시키는 단계; (3) 상기 공동배양한 마늘 세포를 항생제가 첨가된 선발 배지에서 11~13주간 선발하는 단계; 및 (4) 상기 선발된 마늘 세포를 재분화 배지에서 재분화하는 단계를 포함하는 마늘의 형질전환 효율을 증가시키는 방법과 상기 방법에 따라 제조된 마늘 식물체에 관한 것이다.
마늘, 아그로박테리움, 형질전환, 방법, 제초제, 저항성-
公开(公告)号：KR1020000031547A
公开(公告)日：2000-06-05
申请号：KR1019980047646
申请日：1998-11-07
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: PURPOSE: A simple and quick detection method of disease resistance red pepper using DNA marker is provided which selects bacterial spot disease resistant individuals of red pepper independently of environment conditions thus it saves money and time for the selection. CONSTITUTION: DNA pools are made from each 10 resistant and susceptible plant leaves.400 random primer consisted of 10 nucleotides are used to perform PCR and random amplified polymorphic DNA (RAPD) is screened. Two primers (OP3405 and OP3610) showing specific DNA bands to resistant DNA pool are selected as a resistance marker. Genetic analysis using BC1F1 variety reveals that OP3405 is located at 5.4 cm from resistant gene and OP3610 at 9.3 cM. Two RAPD markers are cloned and nucleotide sequence is determined. Using sequences of two marker, sequence specific primers are synthesized and used for amplification of sequence characterized amplified region (SCAR) marker. SCAR markers are specifically amplified from disease resistance plant and used for the selection of disease resistance plant.
Abstract translation: 目的：提供使用DNA标记的抗病性红辣椒的简单快速检测方法，其独立于环境条件选择红斑病的细菌斑点抗病个体，从而节省了选择的时间和资金。 构成：每10个抗性和敏感植物叶片制备DNA库。使用由10个核苷酸组成的随机引物进行PCR，并筛选随机扩增多态性DNA（RAPD）。 选择显示针对抗性DNA库的特异性DNA条带的两个引物（OP3405和OP3610）作为抗性标记。 使用BC1F1品种的遗传分析显示，OP3405位于距抗性基因5.4cm处，在9.3cM处位于OP3610。 克隆两个RAPD标记，确定核苷酸序列。 使用两个标记的序列，合成序列特异性引物并用于扩增序列表征的扩增区（SCAR）标记。 SCAR标记从抗病植物特异性扩增，用于选择抗病性植物。
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公开(公告)号：KR102125521B1
公开(公告)日：2020-07-07
申请号：KR1020190072526
申请日：2019-06-18
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/6895
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