公开(公告)号：KR1020070066352A

公开(公告)日：2007-06-27

申请号：KR1020050127392

申请日：2005-12-21

Applicant: 대한민국(농촌진흥청장)

Inventor： 윤두학 ,  김종주 ,  전진태 ,  박응우 ,  이승환 ,  오성종

IPC: C12Q1/68 ,  G06F19/28

Abstract: A method for identifying Korean native cattle is provided to distinguish the Korean native cattle from imported beef with excellent accuracy by using microsatellite loci, thereby improving income of a farmhouse raising the Korean native cattle and the reliability of consumers. The method comprises the steps of: (a) preparing a PCR reaction solution for amplifying alleles corresponding to microsatellite loci of a selected cow such as ILSTS005, ILSTS028, ILSTS103, ILSTS006, ILSTS008, ILSTS023, ILSTS050, TGLA122, TGLA126, MGTG4B, TGLA227, BM1824, BM2112, ETH10, ETH225, ETH3, and SPS115; (b) amplifying the alleles by performing the multiple PCR reaction thereon using a PCR reaction solution which is a combination of ETH225, ETH10, ETH3, BM2113, BM1824 and SPS115; (c) isolating each of the amplified alleles and then analyzing the size of the each of the alleles; (d) putting the size of the alleles by individuals and species in order and preparing the number and the frequency distribution of the alleles by the species based thereon; and (e) building an information bank of the alleles corresponding to the microsatellite loci to identify the species according to the statistical analysis.

Abstract translation: 提供了一种识别韩国本地牛的方法，通过使用微卫星位点，以极高的准确度将韩国本地牛与进口牛肉区分开来，从而提高了养殖韩国本地牛的农场收入和消费者的可靠性。 该方法包括以下步骤：（a）制备PCR反应溶液，用于扩增相应于所选择的牛的微卫星位点的等位基因，例如ILSTS005，ILSTS028，ILSTS103，ILSTS006，ILSTS008，ILSTS023，ILSTS050，TGLA122，TGLA126，MGTG4B，TGLA227， BM1824，BM2112，ETH10，ETH225，ETH3和SPS115; （b）通过使用作为ETH225，ETH10，ETH3，BM2113，BM1824和SPS115的组合的PCR反应溶液在其上进行多重PCR反应来扩增等位基因; （c）分离每个扩增的等位基因，然后分析每个等位基因的大小; （d）按个人和物种排列等位基因的大小，并根据物种准备等位基因的数量和频率分布; 和（e）建立与微卫星位点相对应的等位基因的信息库，以根据统计分析鉴定该物种。

公开(公告)号：KR101931617B1

公开(公告)日：2018-12-21

申请号：KR1020160150636

申请日：2016-11-11

Applicant: 대한민국(농촌진흥청장)

Inventor： 황성수 ,  박응우 ,  임기순 ,  오건봉 ,  옥선아 ,  양현 ,  곽태욱 ,  이주영

IPC: C12N9/10 ,  C07K16/40 ,  G01N33/68

Abstract: 본 발명은 서열번호 2의 염기서열을 이용하는, 초급성 면역 반응 억제 확인용 항원성 폴리펩티드, 발현벡터, 항체 및 이의 용도에 관한 것이다. 본 발명에 의하면, 초급성 면역반응을 유발하는 인자인 iGb3s를 제어(-/-)한 돼지의 특성 분석 및 iGb3s에 대한 정확한 분석이 가능할 수 있다.

公开(公告)号：KR101099593B1

公开(公告)日：2011-12-28

申请号：KR1020090113983

申请日：2009-11-24

Applicant: 대한민국(농촌진흥청장)

Inventor： 황성수 ,  박수봉 ,  박응우 ,  양병철 ,  오건봉 ,  고응규 ,  곽태욱

IPC: C12N5/07 ,  A01N1/02

Abstract: 본 발명은 체세포를 동결보존하는 방법에 관한 것으로서, 본 발명의 목적은, 진공포장 방법을 이용하여 체세포를 초저온(-70℃~-80℃)에서 보존할 수 있는, 체세포 동결보존 방법을 제공하는 것이다. 이를 위해 본 발명은, 동결튜브(cryotube)에 담겨진 체세포를 동결시키는 단계; 동결된 상기 동결튜브를 필름접착기를 이용하여 진공포장하는 단계; 및 진공포장이 완료된 상기 동결튜브를 초저온 냉동고에 보관하는 단계를 포함한다.
체세포, 동결보존, 진공포장, 냉동고

公开(公告)号：KR1020080006719A

公开(公告)日：2008-01-17

申请号：KR1020060065802

申请日：2006-07-13

Applicant: 대한민국(농촌진흥청장)

Inventor： 박응우 ,  윤두학 ,  이승환 ,  조용민 ,  오성종

IPC: C12Q1/68 ,  G06F19/20

CPC classification number: C12Q1/686 ,  C12Q1/683 ,  G06F19/20

Abstract: A DNA marker is provided to determine an individual genetic trait of Korean native cattle by using genotypes of three independent loci of a FABP4(adipocyte-fatty acid binding protein) gene which has the significantly effect for carcass weight and marbling of the Korean native cattle, thereby being utilized for an early selection of a fatting beef and an accurate estimate of breeding values. A method for selecting a DNA marker of determining genetic traits comprises the steps of: (a) identifying a genotype of a linkage group detected from a Korean native cattle a fatty acid binding protein(FABP4) gene of SEQ ID : NO. 7 using the decoding of 17 SNP loci depicted at a Fig. 1; (b) after pre-denaturing the Korean native cattle FABP4 gene of SEQ ID : NO. 7 as a template using a PCR primer of SEQ ID : NOs. 1 to 6 at a temperature of 94 deg.C for 5 minutes, performing 35 times of a cycle where one minute annealing at a temperature of 58 deg.C and two-minute expanding at a temperature of 72 deg.C are done and then finally reacting it for 10 minutes at a temperature of 72 deg.C for PCR amplification; (c) after cleaving an amplification product obtained from the step(b) with an restriction enzyme such as Hpy188I, RsaI and NlaIII, subjecting it to an electrophoresis; and (d) obtaining a picture and an image of the electrophoresis product to determine an individual genotype through a restriction fragment length polymorphism analysis, a heteroduplex analysis, a single strand conformational polymorphism(SSCP) and a Taqman probe method. A DNA marker is selected by the method and constructed to recognize three loci of Hpy188I(C->G), RsaI(A->T) and NlaIII(A->G) described in the Fig.1 among variable regions of a FAB4 gene expressed in adipocyte.

Abstract translation: 提供DNA标记，通过使用FABP4（脂肪细胞 - 脂肪酸结合蛋白）基因的三个独立基因座的基因型来确定韩国本地牛的个体遗传特征，该基因对韩国本地牛的胴体重量和大理石花纹具有显着影响， 从而用于早期选择肥胖的牛肉和对育种价值的准确估计。 选择确定遗传性状的DNA标记的方法包括以下步骤：（a）鉴定从韩国本土牛检测的连锁群的基因型，其为SEQ ID NO：1的脂肪酸结合蛋白（FABP4）基因。 使用图7所示的17个SNP位点的解码。 1; （b）将SEQ ID NO：1的韩国本地牛FABP4基因预变性后， 7作为模板，使用SEQ ID：NO的PCR引物。 1〜6℃，温度94℃5分钟，进行35次循环，在58℃的温度下进行1分钟的退火，72℃的温度下进行2分钟的膨胀 最后在72℃的温度下反应10分钟进行PCR扩增; （c）用限制酶如Hpy188I，RsaI和NlaIII切割从步骤（b）获得的扩增产物后，进行电泳; 和（d）通过限制性片段长度多态性分析，异源双链分析，单链构象多态性（SSCP）和Taqman探针法获得电泳产物的图片和图像以确定个体基因型。 通过该方法选择DNA标记，并构建为在FAB4的可变区中识别图1中描述的Hpy188I（C-> G），RsaI（A-> A）和NlaIII（A-> G）的三个位点 在脂肪细胞中表达的基因。

公开(公告)号：KR1020070066353A

公开(公告)日：2007-06-27

申请号：KR1020050127393

申请日：2005-12-21

Applicant: 대한민국(농촌진흥청장)

Inventor： 윤두학 ,  이준헌 ,  이승환 ,  권영숙 ,  이경연 ,  박응우 ,  오성종

IPC: C12Q1/68

Abstract: A method for identifying Korean native cattle and imported beef is provided to accurately identify the Korean native cattle derived from Bos taurus and the imported beef derived from Bos indicus by using structural differences of an RFLP genotype of a sex-determining region Y(SRY) gene. The method comprises the steps of: (a) collecting DNA from Korean native cattle and imported beef respectively; (b) respectively combining a forward primer having a nucleotide sequence of 5'-TTAGAACGCTTACACCGCATATTACTTC-3' and a backward primer having a nucleotide sequence of 5'-TAGTAAAATTGAGATAAAGAGCGCCTTTGTTAG-3' of an SRY gene with each of the collected DNA; (c) amplifying the combined product through PCR in which a nucleotide sequence portion of 1003-1803 in a Bos taurus DNA(registration number : AB039748) and a nucleotide sequence portion of 273-1073 of a Bos indicus DNA(registration number : AY079145) are amplified; and (d) after cutting the amplified product with an MseI restriction enzyme, analyzing polymorphism thereof to divide genotypes of the Korean native cattle and the imported beef.

Abstract translation: 提供了一种识别韩国本地牛和进口牛肉的方法，通过使用性别决定区Y（SRY）基因的RFLP基因型的结构差异来准确地识别来自Bos金牛的韩国本地牛和来自Bos indicus的进口牛肉 。 该方法包括以下步骤：（a）分别从韩国本地牛和进口牛收集DNA; （b）分别组合具有5'-TTAGAACGCTTACACCGCATATTACTTC-3'的核苷酸序列的正向引物和具有SRY基因的5'-TAGTAAAATTGAGATAAAGAGCGCCTTGTTAG-3'的核苷酸序列的反向引物与每个收集的DNA的组合; （c）通过PCR扩增组合产物，其中Bos Taurus DNA（登记号：AB039748）中的1003-1803的核苷酸序列部分和Bos indicus DNA的273-1073的核苷酸序列部分（登记号：AY079145） 被放大; 和（d）用MseI限制酶切割扩增产物后，分析其多态性以分割韩国本地牛和进口牛肉的基因型。