公开(公告)号：KR1020040012399A

公开(公告)日：2004-02-11

申请号：KR1020020045969

申请日：2002-08-03

Applicant: 대한민국(농촌진흥청장) ,  학교법인 성균관대학

Inventor： 이강노 ,  조세연 ,  지상덕 ,  권학철

IPC: C07D209/36

Abstract: PURPOSE: Provided are an extract of Paecilomyces sp. J300 having antitumor activity and a pharmaceutical composition containing the same. The extract contains novel antitumor matters, and is thus useful for an antitumor drug composition. CONSTITUTION: A extract of Paecilomyces sp. J300 having antitumor activity contains, as antitumor matters, 5,3-dihydroxy-1-N-methyl-indoline-2-carbonylamino-alanyl-hydoxyisoleucinyl-glycine represented by the formula(1) or its derivatives. An antitumor drug composition contains one of 5,3-dihydroxy-1-N-methyl-indoline-2-carbonylamino-alanyl-hydoxyisoleucinyl-glycine or its derivatives including 5 alpha, 6 alpha-epoxy-(22E,24R)-ergosta-8(14), 22-diene-3 beta, 7 alpha-diol of the formula(2), 5 alpha, 6 alpha-epoxy-(22E, 24R)-ergosta-8, 22-diene-3 beta, 7 alpha-diol of the formula(3), ergosta-4, 6, 8(14), 22-tetraene-3-one of the formula(4) and 3 beta, 5 alpha-dihydroxy-6 beta-methoxyergosta-7, 22-diene of the formula(5).

Abstract translation: 目的：提供拟青霉菌属 J300具有抗肿瘤活性和含有其的药物组合物。 提取物含有新的抗肿瘤物质，因此可用于抗肿瘤药物组合物。 构成：拟青霉菌提取物 具有抗肿瘤活性的J300含有作为抗肿瘤物质的由式（1）表示的5,3-二羟基-1-N-甲基 - 二氢吲哚-2-羰基氨基 - 丙氨酰 - 羟基异丁烯基 - 甘氨酸或其衍生物。 一种抗肿瘤药物组合物包含5,3-二羟基-1-N-甲基 - 二氢吲哚-2-羰基氨基 - 丙氨酰 - 羟基异亮氨酰基甘氨酸或其衍生物之一，包括5α，6α-环氧 - （22E，24R） 8（14），22-二烯-3β，7α-二醇，5α，6α-环氧 - （22E，24R） - 麦角甾-8,22-二烯-3β，7α 式（3）的二醇，式（4）的麦角胺-4,6,8（14），22-四烯-3-酮和3β，5α-二羟基-6β-甲氧麦角甾-7,22 - 二烯（式（5））。

公开(公告)号：KR1020030012998A

公开(公告)日：2003-02-14

申请号：KR1020010047271

申请日：2001-08-06

Applicant: 대한민국(농촌진흥청장)

Inventor： 지상덕 ,  조세연 ,  남성희 ,  송병권 ,  이효순

IPC: A01G1/04

CPC classification number: A61K36/068

Abstract: PURPOSE: A method for culturing Isaria sinclairii is provided by inoculating Isaria sinclairii to the pupa or larva of a domestic silkworm, thereby mass producing Isaria sinclairii . CONSTITUTION: The method for culturing Isaria sinclairii using a domestic silkworm comprises the steps of: inoculating spores of Isaria sinclairii to the larva or pupa of a domestic silkworm by spraying the spores on the surface of the larva or the pupa; and forming a fruit body of Isaria sinclairii from the infected pupa, wherein the inoculation process is carried out by diluting the spores of Isaria sinclairii in water in the concentration of 106/ml to 108/ml, spraying the diluted solution on the surfaces of the larvae or pupae of the domestic silkworms aged 2 weeks, 3 weeks, 4 weeks and 5 weeks, and leaving them under 95% of humidity for 12 hours; the larvae or pupae of the domestic silkworms are stored at 20 deg. C or less prior to the inoculation; and the fruit body formation process is carried out under conditions of 15 to 25 deg. C and humidity of 95%.

Abstract translation: 目的：通过将Isaria sinclairii接种到家蚕的蛹或幼虫来提供Isaria sinclairii的培养方法，从而大量生产Isaria sinclairii。 构成：使用家蚕培养伊蚊（Isaria sinclairii）的方法包括以下步骤：通过将孢子喷洒在幼虫或蛹的表面上，将Isaria sinclairii的孢子接种到家蚕的幼虫或蛹中; 并从感染的蛹中形成Isaria sinclairii的果实体，其中通过在浓度为106 / ml至108 / ml的水中稀释Isaria sinclairii的孢子进行接种过程，将稀释的溶液喷洒在 家蚕的幼虫或蛹2周，3周，4周和5周，并将其置于95％湿度下12小时; 家蚕的幼虫或蛹保存在20度。 C以下; 果实体形成处理在15〜25℃的条件下进行。 C和湿度95％。

公开(公告)号：KR1020020067367A

公开(公告)日：2002-08-22

申请号：KR1020010007899

申请日：2001-02-16

Applicant: 대한민국(농촌진흥청장)

Inventor： 남성희 ,  조세연 ,  정이연 ,  지상덕 ,  최영숙

IPC: A01G1/04

CPC classification number: C12N1/14

Abstract: PURPOSE: Provided is a preparation method of a medium for Entomopathogenic fungi effectively and economically by using silkworm powder used as a hypoglycemic agent. The medium increases the growth rate of Entomopathogenic fungi. CONSTITUTION: The medium for Entomopathogenic fungi is characterized by dissolving 24g of silkworm powder in 1L of distilled water; sterilizing it at 121 deg.C for 15 minutes; filtering the sterilized solution with gauze and a sieve sequentially; adding 15g of agar the pure solution; sterilizing it; and pipetting 21mL of the solution into each petridish the solidifying them.

Abstract translation: 目的：通过使用用作降血糖药的蚕粉，有效和经济地提供了一种用于昆虫病原真菌的培养基的制备方法。 培养基增加了真菌致病真菌的生长速度。 构成：昆虫病原真菌的特征是将24g蚕粉溶解在1L蒸馏水中; 在121℃下灭菌15分钟; 用纱布和筛子依次过滤灭菌液; 加入15g琼脂纯溶液; 消毒; 并将21mL的溶液移液到每个头皮中固化。

公开(公告)号：KR100187897B1

公开(公告)日：1999-06-01

申请号：KR1019960062054

申请日：1996-12-05

Applicant: 대한민국(농촌진흥청장)

Inventor： 조세연 ,  지상덕 ,  임수호

IPC: A01G1/04

Abstract: 본 발명은 항암·면역증강 등 각종 효능이 뛰어난 것으로 알려져 있으나 자연에서의 채집이 극히 어려운 관계로 인간에게 유용하게 이용되지 못하고 있는 동충하초(Paecilomyces japonica)를 인공재배하는 방법에 관한 것으로, 동충하초의 일종인 페실로마이세스 자포니카균 포자를 분무기를 이용하여 가잠의 4∼5령기 유충 또는 번데기 표피에 경피 접종한 후, 균사체를 형성한 번데기에서 자실체를 형성시키는 것을 특징으로 한다.

公开(公告)号：KR1020050045982A

公开(公告)日：2005-05-17

申请号：KR1020050036489

申请日：2005-04-30

Applicant: 문창현 ,  대한민국(농촌진흥청장)

Inventor： 안미영 ,  조세연 ,  지상덕 ,  이용기 ,  문창현 ,  정이숙 ,  이보경 ,  김찬식

IPC: A23L1/29

CPC classification number: A23L33/00 ,  A23L2/38 ,  A23L33/105

Abstract: 본 발명은 애매미유충눈꽃동충하초 자체, 수용성 추출물 또는 알콜 추출물을 유효성분으로 하는 혈압강하용 조성물 및 체중감소용 조성물에 관한 것이다.
본 발명의 조성물은 혈압상승에 의해 야기되는 각종 질환 및 체중증가에 의해 야기되는 각종 질환의 예방 및 치료제로 사용할 수 있다.

公开(公告)号：KR1019980025198A

公开(公告)日：1998-07-06

申请号：KR1019970065095

申请日：1997-12-01

Applicant: 대한민국(농촌진흥청장)

Inventor： 지상덕 ,  임수호 ,  조세연

IPC: A01G1/04

Abstract: 본 발명은 항암·면역증강 등 각종 효능이 뛰어난 것으로 알려져 있으나, 자연에서의 채집이 극히 어려운 관계로 인간에게 유용하게 이용되지 못하고 있는 동충하초(Cordyceps militaris)를 인공재배하는 방법에 관한 것으로, 동충하초의 일종인 코디셉스 밀리타리스의 포자를 가잠의 5령기 유충 표피에 분무 접종한 후, 균사체를 형성한 번데기에서 자실체를 형성시키는 것을 특징으로 한다.

公开(公告)号：KR1020110127324A

公开(公告)日：2011-11-25

申请号：KR1020100046741

申请日：2010-05-19

Applicant: 한림대학교 산학협력단 ,  인하대학교 산학협력단 ,  대한민국(농촌진흥청장)

Inventor： 최수영 ,  음원식 ,  박진서 ,  장상호 ,  김대원 ,  조용준 ,  조성민 ,  신승훈 ,  권해용 ,  이광길 ,  강석우 ,  진형준 ,  조세연

IPC: A61F2/02 ,  A61L27/22 ,  C08J3/205 ,  C08L89/00

CPC classification number: A61F2/02 ,  A61L27/227 ,  C08J3/205 ,  C08L89/00

Abstract: PURPOSE: An artificial dura mater and a manufacturing method thereof are provided to guarantee safety in operation and to do not cause inflammation in the operation. CONSTITUTION: Silk fibroin aqueous solution is cast in smooth and flat place and is dried. The crystallization induction of the dried silk fibroin is executed. A crystallization induction step puts the dried silk fibroin is executed into an enclosure container in which relative humidity is over 80% and executes annealing with water.

Abstract translation: 目的：提供人造硬膜及其制造方法，以保证手术中的安全性，并且不会在手术中引起炎症。 构成：将丝素蛋白水溶液浇铸在光滑平坦的地方，并干燥。 干燥的丝素蛋白的结晶诱导被执行。 结晶诱导步骤将干燥的丝素蛋白进行到相对湿度超过80％的封闭容器中，并用水进行退火。

公开(公告)号：KR100407072B1

公开(公告)日：2003-11-28

申请号：KR1020010047271

申请日：2001-08-06

Applicant: 대한민국(농촌진흥청장)

Inventor： 지상덕 ,  조세연 ,  남성희 ,  송병권 ,  이효순

IPC: A01G1/04

Abstract: PURPOSE: A method for culturing Isaria sinclairii is provided by inoculating Isaria sinclairii to the pupa or larva of a domestic silkworm, thereby mass producing Isaria sinclairii . CONSTITUTION: The method for culturing Isaria sinclairii using a domestic silkworm comprises the steps of: inoculating spores of Isaria sinclairii to the larva or pupa of a domestic silkworm by spraying the spores on the surface of the larva or the pupa; and forming a fruit body of Isaria sinclairii from the infected pupa, wherein the inoculation process is carried out by diluting the spores of Isaria sinclairii in water in the concentration of 106/ml to 108/ml, spraying the diluted solution on the surfaces of the larvae or pupae of the domestic silkworms aged 2 weeks, 3 weeks, 4 weeks and 5 weeks, and leaving them under 95% of humidity for 12 hours; the larvae or pupae of the domestic silkworms are stored at 20 deg. C or less prior to the inoculation; and the fruit body formation process is carried out under conditions of 15 to 25 deg. C and humidity of 95%.

Abstract translation: 目的：通过将Isaria sinclairii接种到家蚕的蛹或幼虫来提供Isaria sinclairii的培养方法，由此大量生产Isaria sinclairii。 组成：使用家蚕培养Isaria sinclairii的方法包括以下步骤：通过将孢子喷洒在幼虫或蛹表面上，将Isaria sinclairii的孢子接种到家蚕的幼虫或蛹中; 并从感染的蛹中形成Isaria sinclairii的果实体，其中接种过程通过将Isaria sinclairii的孢子以106 / ml的浓度稀释到10 8 / ml，将稀释的溶液喷雾到 2周，3周，4周和5周的家蚕的幼虫或蛹，并将其置于95％的湿度下12小时; 家蚕的幼虫或蛹储存在20℃。 接种前C或更少; 并且在15至25度的条件下进行子实体形成过程。 C和95％的湿度。

公开(公告)号：KR1020020078322A

公开(公告)日：2002-10-18

申请号：KR1020010018664

申请日：2001-04-09

Applicant: 대한민국(농촌진흥청장) ,  학교법인 한동대학교

Inventor： 조세연 ,  지상덕 ,  송성규 ,  이관희

IPC: C07D241/08

Abstract: PURPOSE: Provided are 3-(5-(methoxy-ethyl)-3,6-dioxo-piperazine-2-yl)-propionic acid of the formula(1) extracted and purified from the J300 Cordyceps militaris, and a pharmaceutical composition having anti-HIV activity, an agent for treating AIDS and a supplementary food containing the same. CONSTITUTION: The 3-(5-(methoxy-ethyl)-3,6-dioxo-piperazine-2-yl)-propionic acid of the formula(1) is manufactured by the steps of: extracting Cordyceps militaris powders with water; performing HPLC with the extract using distilled water; isolating the distilled water fraction by HPLC using methanol; and isolating the methanol fraction by activated carbon chromatography to obtain J300 Cordyceps militaris water extract Dong1-2-4 having anti-HIV activity.

Abstract translation: 目的：提供从J300冬虫夏草提取和纯化的式（1）的3-（5-（甲氧基 - 乙基）-3,6-二氧代 - 哌嗪-2-基） - 丙酸和具有 抗艾滋病毒活动，治疗艾滋病的药物和含有艾滋病毒的补充食品。 构成：式（1）的3-（5-（甲氧基 - 乙基）-3,6-二氧代 - 哌嗪-2-基） - 丙酸通过以下步骤制备：用水提取冬虫夏草粉末; 使用蒸馏水用提取物进行HPLC; 使用甲醇通过HPLC分离蒸馏水部分; 并通过活性炭色谱分离甲醇馏分，得到具有抗HIV活性的J300冬虫夏草水提取物Dong1-2-4。

公开(公告)号：KR100307670B1

公开(公告)日：2001-09-13

申请号：KR1019990016428

申请日：1999-05-08

Applicant: 대한민국(농촌진흥청장)

Inventor： 김삼은 ,  조세연 ,  최지영 ,  지상덕 ,  김종길 ,  임수호

IPC: A01G1/04

Abstract: 본발명은가잠을이용하여동충하초(冬蟲夏草)를인공재배하는방법에있어서, 가잠대신에나용() 돌연변이누에를기주곤충으로이용하므로써고치를절개하여감염번데기를선별하는종래의수작업과정을생략할수 있어노동력을절감할수 있으며, 자실체의형성율또한향상시킬수 있는방법에관한것이다.