Abstract:
A gene encoding pest resistant defensin derivered from brassica rapa is provided to produce a transgenic plant body having resistance to biological stress including mold, virus, bacteria, globodera rostochiensis and pest and abiotic stress including drying, injury from salt, and heavy metal. A recombinant vector pMJC-GB-BrD1 is prepared by inserting a pest resistant defensin gene BrD1 derivered from brassica rapa, into pSB1. A transgenic body is obtained by introducing the recombinant vector pMJC-GB-BrD1 to Agrobacterium tumefaciens. A transgenic plant body is obtained by introducing the transgenic body to plants.
Abstract:
A vector comprising hrp(hypersensitive response and pathogenicity) gene encoding harpin protein, derived from Erwinia pyrifoliae, and a transgenic Agrobacterium tumefaciens and a pathogenic resistant transgenic plant by using the same vector are provided to activate the pathogenic resistant mechanisms of the plant itself without inducing hypersensitive response and replace toxic agricultural chemicals. The vector pMJC-GB-hrpEp comprises hrpEP gene derived from Erwinia pyrifoliae wild type #3 KCCM 10283. The transgenic Agrobacterium tumefaciens is produced by transforming Agrobacterium tumefaciens by the vector pMJC-GB-hrpEp. The pathogenic resistant transgenic plant is produced by transforming a plant by the vector pMJC-GB-hrpEp or the transgenic Agrobacterium tumefaciens, wherein the plant is tobacco or rice.