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公开(公告)号：KR1020120027991A
公开(公告)日：2012-03-22
申请号：KR1020100089922
申请日：2010-09-14
Applicant: 삼성전자주식회사
CPC classification number: C12Q1/6806 , C12Q1/686 , C12Q1/25 , C12Q2527/125
Abstract: PURPOSE: A method for enhancing PCR sensitivity and speed and a composition for the same are provided to quickly detect target microorganisms, cells or genes in a sample. CONSTITUTION: A method for enhancing PCR efficiency and speed comprises: a step of preparing a biological sample and PCR mixture and adding alkali to one or both; and a step of mixing the biological sample and PCR mixture and performing PCR. The biological sample includes cells, body fluid, or nucleic acids. The alkali is sodium hydroxide or potassium hydroxide. A composition for efficient PCR contains the PCR mixture and alkali.
Abstract translation: 目的：提供增强PCR灵敏度和速度的方法及其组合物，以快速检测样品中的目标微生物，细胞或基因。 构成：提高PCR效率和速度的方法包括：制备生物样品和PCR混合物并向一种或两种添加碱的步骤; 以及混合生物样品和PCR混合物并进行PCR的步骤。 生物样品包括细胞，体液或核酸。 碱是氢氧化钠或氢氧化钾。 用于高效PCR的组合物含有PCR混合物和碱。
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公开(公告)号：KR1020100027698A
公开(公告)日：2010-03-11
申请号：KR1020080086711
申请日：2008-09-03
Applicant: 삼성전자주식회사
IPC: G01N35/00
CPC classification number: B01L3/502715 , B01L2200/16 , B01L2300/0806 , B01L2300/0816 , B01L2300/087 , B01L2300/1833 , B01L2300/1866 , B01L2400/0409 , Y10T436/25125
Abstract: PURPOSE: A dielectric heating method using microwaves and anions is provided to reduce heating time of objects by increasing a heating rate through the addition of negative ions with high charge density promoting polarization of molecule. CONSTITUTION: A dielectric heating method using microwaves and anions comprises the steps of: adding negative ions with high charge density, which interacts with a medium molecule strongly than a hydrogen bond between medium molecules of dielectric, to a dielectric for dielectric heating; and irradiating microwaves to the dielectric to heat the dielectric.
Abstract translation: 目的：提供使用微波和阴离子的电介质加热方法，通过加入具有高电荷密度的负离子促进分子的极化，从而通过增加加热速率来减少物体的加热时间。 构成：使用微波和阴离子的电介质加热方法包括以下步骤：将具有高电荷密度的负离子与媒介分子强烈地相互作用于电介质介质分子之间的氢键; 并向该电介质照射微波以加热电介质。
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公开(公告)号：KR100647335B1
公开(公告)日：2006-11-23
申请号：KR1020050082442
申请日：2005-09-05
Applicant: 삼성전자주식회사
Abstract: 본 발명은 물 접촉각(water contact angle)이 70~90도인 소수성 고체 지지체에 pH 2.5~4의 조건에서 세포 또는 바이러스를 포함한 용액을 접촉시키는 단계를 포함하는 소수성 고체 지지체를 이용한 세포 또는 바이러스의 분리 방법에 관한 것이다. 본 발명에 따르면, 소수성 고체 지지체에 단지 세포 또는 바이러스를 흡착시킴으로써 세포 또는 바이러스를 분리할 수 있으므로, 신속하고 간단하게 세포 또는 바이러스 분리 효율을 증가시킬 수 있다.
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公开(公告)号：KR100601985B1
公开(公告)日：2006-07-18
申请号：KR1020050006577
申请日：2005-01-25
Applicant: 삼성전자주식회사
Abstract: 본 발명은 세포 또는 바이러스를 포함한 용액을 알루미나에 접촉시키는 단계; 및 상기 세포 또는 바이러스가 결합된 알루미나를 세정하는 단계를 포함하는 알루미나를 이용한 세포 또는 바이러스의 분리 방법에 관한 것이다. 본 발명에 따르면, 세포 또는 바이러스의 분리 효율을 증가시키고, 세포 또는 바이러스에 대한 검출 한계를 향상시키고, 신속하게 시료를 제조할 수 있다.
Abstract translation: 本发明涉及生产细胞的方法，包括：使含有细胞或病毒的溶液与氧化铝接触; 用氧化铝洗涤细胞或病毒结合的氧化铝。 根据本发明，可以提高细胞或病毒的分离效率，提高对细胞或病毒的检测限，并且可以快速制备样品。
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Patent Agency Ranking16.发明公开Ｂ형 간염 검사용 ＰＣＲ 프라이머 세트 및 이를 포함하는 Ｂ형 간염 검사 키트 有权用于检测乙型肝炎病毒的PCR检测装置将检测时间减少到30分钟，并提高检测重现性和用于检测乙型肝炎病毒B的方法
公开(公告)号：KR1020050018511A
公开(公告)日：2005-02-23
申请号：KR1020030056432
申请日：2003-08-14
Applicant: 삼성전자주식회사
IPC: C12Q1/68
CPC classification number: C12Q1/706 , C12Q2565/629 , C12Q2565/543 , C12Q2531/113
Abstract: PURPOSE: A PCR primer set for detection of hepatitis B and a method for detecting hepatitis B using the same primer set are provided, thereby reducing the detection time to 30 minutes and improving the detection reproductivity by optimizing the PCR condition and using specific PCR primer set. CONSTITUTION: The PCR primer set for detection of hepatitis B is selected from the group consisting of: (a) a set of primers having the nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:2; (b) a set of primers having the nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:3; (c) a set of primers having the nucleotide sequences of SEQ ID NO:4 and SEQ ID NO:5; (d) a set of primers having the nucleotide sequences of SEQ ID NO:4 and SEQ ID NO:6; (e) a set of primers having the nucleotide sequences of SEQ ID NO:4 and SEQ ID NO:7; (f) a set of primers having the nucleotide sequences of SEQ ID NO:7 and SEQ ID NO:8; (g) a set of primers having the nucleotide sequences of SEQ ID NO:9 and SEQ ID NO:10; (h) a set of primers having the nucleotide sequences of SEQ ID NO:11 and SEQ ID NO:12; (i) a set of primers having the nucleotide sequences of SEQ ID NO:13 and SEQ ID NO:14; (j) a set of primers having the nucleotide sequences of SEQ ID NO:15 and SEQ ID NO:16; (k) a set of primers having the nucleotide sequences of SEQ ID NO:17 and SEQ ID NO:18; (l) a set of primers having the nucleotide sequences of SEQ ID NO:19 and SEQ ID NO:20; (m) a set of primers having the nucleotide sequences of SEQ ID NO:21 and SEQ ID NO:22; (n) a set of primers having the nucleotide sequences of SEQ ID NO:23 and SEQ ID NO:24; (o) a set of primers having the nucleotide sequences of SEQ ID NO:25 and SEQ ID NO:26; (p) a set of primers having the nucleotide sequences of SEQ ID NO:27 and SEQ ID NO:28; (q) a set of primers having the nucleotide sequences of SEQ ID NO:29 and SEQ ID NO:30; (r) a set of primers having the nucleotide sequences of SEQ ID NO:31 and SEQ ID NO:32; (s) a set of primers having the nucleotide sequences of SEQ ID NO:33 and SEQ ID NO:34; (t) a set of primers having the nucleotide sequences of SEQ ID NO:35 and SEQ ID NO:36; (u) a set of primers having the nucleotide sequences of SEQ ID NO:37 and SEQ ID NO:38; and (a) a set of primers having the nucleotide sequences of SEQ ID NO:39 and SEQ ID NO:40.
Abstract translation: 目的：提供用于检测乙型肝炎的PCR引物组和使用相同引物组检测乙型肝炎的方法，从而将检测时间缩短到30分钟，并通过优化PCR条件并使用特异性PCR引物组来提高检测繁殖力 。 构成：用于检测乙型肝炎的PCR引物组选自：（a）一组具有SEQ ID NO：1和SEQ ID NO：2的核苷酸序列的引物; （b）具有SEQ ID NO：1和SEQ ID NO：3的核苷酸序列的一组引物; （c）具有SEQ ID NO：4和SEQ ID NO：5的核苷酸序列的一组引物; （d）具有SEQ ID NO：4和SEQ ID NO：6的核苷酸序列的一组引物; （e）具有SEQ ID NO：4和SEQ ID NO：7的核苷酸序列的一组引物; （f）具有SEQ ID NO：7和SEQ ID NO：8的核苷酸序列的一组引物; （g）具有SEQ ID NO：9和SEQ ID NO：10的核苷酸序列的一组引物; （h）具有SEQ ID NO：11和SEQ ID NO：12的核苷酸序列的一组引物; （i）具有SEQ ID NO：13和SEQ ID NO：14的核苷酸序列的一组引物; （j）具有SEQ ID NO：15和SEQ ID NO：16的核苷酸序列的一组引物; （k）具有SEQ ID NO：17和SEQ ID NO：18的核苷酸序列的一组引物; （1）具有SEQ ID NO：19和SEQ ID NO：20的核苷酸序列的一组引物; （m）具有SEQ ID NO：21和SEQ ID NO：22的核苷酸序列的一组引物; （n）具有SEQ ID NO：23和SEQ ID NO：24的核苷酸序列的一组引物; （o）具有SEQ ID NO：25和SEQ ID NO：26的核苷酸序列的一组引物; （p）一组具有SEQ ID NO：27和SEQ ID NO：28的核苷酸序列的引物; （q）具有SEQ ID NO：29和SEQ ID NO：30的核苷酸序列的一组引物; （r）具有SEQ ID NO：31和SEQ ID NO：32的核苷酸序列的一组引物; 一组具有SEQ ID NO：33和SEQ ID NO：34的核苷酸序列的引物; （t）具有SEQ ID NO：35和SEQ ID NO：36的核苷酸序列的一组引物; （u）具有SEQ ID NO：37和SEQ ID NO：38的核苷酸序列的一组引物; 和（a）具有SEQ ID NO：39和SEQ ID NO：40的核苷酸序列的一组引物。
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公开(公告)号：KR100436554B1
公开(公告)日：2004-06-18
申请号：KR1020010020573
申请日：2001-04-17
Applicant: 삼성전자주식회사
IPC: C12Q1/68
CPC classification number: C12Q1/6834 , C12Q2521/307
Abstract: The present invention provides a method for improving detection sensitivity of hybridized nucleic acid immobilized on a solid support of sensing device for gene assay, by removing non-hybridized nucleic acid probe using nuclease from the solid support. In accordance with the invented method, background signal caused by single stranded probe that is not hybridized with target nucleic acid or signal caused by non-specific binding of target nucleic acid to probe is decreased or removed, which improves detection sensitivity of hybridization with a high accuracy, and minimizes the loss of hybridized nucleic acid in the course of washing background signal removed in the conventional method.
Abstract translation: 本发明提供了一种通过使用来自固体支持物的核酸酶去除未杂交的核酸探针来提高固定在用于基因测定的感测装置的固体支持物上的杂交核酸的检测灵敏度的方法。 根据本发明的方法，由与靶核酸不杂交的单链探针引起的背景信号或由靶核酸与探针的非特异性结合引起的信号减少或消除，这提高了杂交的检测灵敏度， 准确性，并且使在常规方法中去除背景信号的洗涤过程中杂交核酸的损失最小化。
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公开(公告)号：KR1020020080803A
公开(公告)日：2002-10-26
申请号：KR1020010020573
申请日：2001-04-17
Applicant: 삼성전자주식회사
IPC: C12Q1/68
CPC classification number: C12Q1/6834 , C12Q2521/307
Abstract: PURPOSE: Provided is a method for improving a sensitivity of a sensing device in the detection of a hybridized nucleic acid by removing non-hybridized nucleic acid probes using a nuclease. CONSTITUTION: A sensitivity of a sensing device in the detection of hybridization of nucleic acids is improved by removing a non-hybridized nucleic acid probe, wherein the nucleic acids are fixed onto a base plate which is characteristically made of glass, quartz, silicon or plastic.
Abstract translation: 目的：提供了通过使用核酸酶去除非杂交核酸探针来提高感测装置在检测杂交核酸中的灵敏度的方法。 构成：通过去除非杂交的核酸探针来改善感测装置在检测核酸杂交中的灵敏度，其中核酸被固定在特征性地由玻璃，石英，硅或塑料制成的基板上 。
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公开(公告)号：KR101776215B1
公开(公告)日：2017-09-08
申请号：KR1020100107014
申请日：2010-10-29
Applicant: 삼성전자주식회사
CPC classification number: C12Q1/6806 , C12M1/33 , C12M47/06
Abstract: 세포파쇄용마이크로디바이스및 이를이용한세포파쇄방법에관해개시되어있다. 마이크로디바이스는제1 챔버, 제2 챔버, 상기제1 및제2 챔버사이의플렉시블멤브레인및 상기제1 및제2 챔버중 어느하나에구비된, 세포파쇄를위한마이크로수단을포함한다. 상기제1 및제2 챔버중 나머지는상기멤브레인을진동시키기위한가압및 감압챔버일수 있다. 상기마이크로수단은복수의마이크로비드일수 있다. 상기플렉시블멤브레인을진동시켜상기마이크로수단을진동시킬수 있고, 상기마이크로수단의진동과정에서세포는파쇄될수 있다.
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公开(公告)号：KR1020120063162A
公开(公告)日：2012-06-15
申请号：KR1020100124231
申请日：2010-12-07
Applicant: 삼성전자주식회사
CPC classification number: B01L7/52 , B01L3/502715 , B01L2200/027 , B01L2200/028 , B01L2200/04 , B01L2200/0605 , B01L2200/0621 , B01L2200/0684 , B01L2200/10 , B01L2200/16 , B01L2300/0627 , B01L2300/0816 , B01L2300/087 , B01L2300/0874 , B01L2300/0887 , B01L2300/18 , B01L2400/0487 , B01L2400/06 , B01L2400/0655 , C12Q1/68 , G01N21/75 , C12Q1/6851
Abstract: PURPOSE: A gene analysis apparatus and a method for gene analysis using the same are provided to prevent contamination by foreign materials and to enhance accuracy and reliability. CONSTITUTION: A gene analysis apparatus comprises: a chamber(40) for crushing cells; a pump(40P) for pumping the crushed cells by a microchannel(40C); microchannels(50C,52C,54C,56C) for transferring amplification reagent into first to fourth PCR chambers(60,62,64,66); pumps(50P,52P,54P,56P) for pumping a PCR mixture to each microchannel; pumps(60P,62P,64P,66P) for injecting crushed cells and PCR mixture into the first to fourth PCR chambers; and a micro valves(40V,50V,60V) for controlling fluid flow.
Abstract translation: 目的：提供基因分析装置和使用其的基因分析方法，以防止异物污染，提高准确性和可靠性。 构成：基因分析装置包括：用于破碎细胞的室（40）; 用于通过微通道（40C）泵送破碎的电池的泵（40P）; 用于将扩增试剂转移到第一至第四PCR室的微通道（50℃，52℃，54℃，56℃）（60,62,64,66）; 用于将PCR混合物泵送到每个微通道的泵（50P，52P，54P，56P） 用于将破碎细胞和PCR混合物注入第一至第四PCR室的泵（60P，62P，64P，66P） 以及用于控制流体流动的微型阀（40V，50V，60V）。
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