Abstract:
PURPOSE: A modified microorganism for producing lactic acids and a method for producing lactic acids are provided to produce lactic acids with high efficiency under an acidic condition. CONSTITUTION: A modified microorganism for producing lactic acids with high efficiency has LDH activity of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, and Rattus norvegicus. The modified microorganism is yeast or bacteria. The modified microorganism is E.coli or Kluyveromyces marxianus. The modified microorganism produces lactic acids with 12.2% or more of glucose. An expression vector for producing the modified microorganism comprises: a replication origin; a promoter; a polynucleotide; and a terminator. A method for producing lactic acids comprises: a step of culturing the modified microorganism in a medium containing glucose; and a step of collecting lactic acids from the culture.
Abstract:
PURPOSE: A modified microorganism for producing lactic acids is provided to prepare lactic acids with high efficiency under an acidic condition. CONSTITUTION: A modified microorganism for producing lactic acids has a lactate dehydrogenase(LDH) activity of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, or Rattus norvegicus. The modified microorganism is Escherichia sp. or Kluyveromyces sp. The modified microorganism produces lactic acids with 34% or more of glucose. An expression vector contains: a replication origin for constructing the modified microroganisms; a promoter; a polynucleotide coding LDH activation of one or more species selected from the group consisting of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, or Rattus norvegicus; and a terminator. The replication origin is ARS/CEN replication origin.
Abstract:
The present invention provides: a bacteria cell that has at least one increased activity of pathway for transforming alpha-ketoglutrate into succinic acid semialdehyde (SSA) and of succinyl semialdehyde dehydrogenase, and is genetically modified; and a method for producing succinic acid using the same. When the bacterial cell is used, succinic acid can be efficiently produced.