公开(公告)号：KR101569210B1

公开(公告)日：2015-11-23

申请号：KR1020080106278

申请日：2008-10-29

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 유병조 ,  박재찬 ,  구현민 ,  진용수 ,  이기성

IPC: C12N15/52 ,  C12N15/56 ,  C12N15/09

CPC classification number: C12N15/1034 ,  C07K14/395 ,  C12P7/04 ,  C12P7/06 ,  C12P7/16 ,  C12P7/28 ,  Y02E50/10 ,  Y02E50/17

Abstract: 과발현시갈락토오스의대사이용성을증대시킬수 있는유전자에관한기술을제공한다. 구체적인예에서, 과발현시갈락토오스의대사이용성을증가시키는유전자로서, SNR84 유전자를제공한다. SNR84 유전자는과발현됨으로써갈락토오스의대사율을증가시킬수 있으므로, 갈락토오스를함유하는탄소원으로부터바이오알코올의생산성을크게증가시킬수 있다.

公开(公告)号：KR101882740B1

公开(公告)日：2018-07-27

申请号：KR1020120061819

申请日：2012-06-08

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 정순천 ,  구현민 ,  김재영 ,  김지은 ,  김진우 ,  박영경 ,  이소영 ,  조화영 ,  권대혁 ,  박재찬

IPC: C12N15/52 ,  C12P7/56 ,  C12N15/81 ,  C12N15/74

Abstract: 젖산의고효율생산을위한변형미생물, 상기변형미생물을제작하기위한발현벡터, 및상기변형미생물을이용한젖산의생산방법이개시된다. 상기변형미생물은산성조건하에서도고효율로젖산을생산할수 있다.

公开(公告)号：KR1020130124065A

公开(公告)日：2013-11-13

申请号：KR1020120047683

申请日：2012-05-04

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 유병조 ,  구현민 ,  박성민 ,  권대혁 ,  김재영 ,  박재찬

IPC: C12N1/19 ,  C12N15/55 ,  C12P7/06 ,  C12N15/81

CPC classification number: Y02E50/17 ,  C12N15/815 ,  C12N9/2402 ,  C12P7/10 ,  C12P7/40 ,  C12P13/04 ,  C12P19/02 ,  C12P19/04

Abstract: Disclosed is modified microorganisms for simultaneous saccharification and fermentation, an expression vector for preparing the modified microorganisms, and a method for producing chemical materials using the modified microorganisms. According to one side, disclosed is modified Kluyveromyces marxianus which produces chemical materials by simultaneous saccharification and fermentation and contains a replication origin; a promoter; a gene encodes one or more cellulose decomposing enzymes selected among beta-glucosidase, endoglucanase, exoglucanase, and cellobiohydrolase; and a terminator.

Abstract translation: 公开了用于同时糖化和发酵的改性微生物，用于制备改性微生物的表达载体，以及使用改性微生物生产化学物质的方法。 一方面，公开了通过同时进行糖化和发酵生产化学物质的马克斯克鲁维酵母（Kluyveromyces marxianus），并含有复制起点; 启动子 一种基因编码一种或多种选自β-葡糖苷酶，内切葡聚糖酶，外切葡聚糖酶和纤维二糖水解酶的纤维素分解酶; 和终结者。

公开(公告)号：KR1020100047407A

公开(公告)日：2010-05-10

申请号：KR1020080106278

申请日：2008-10-29

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 유병조 ,  박재찬 ,  구현민 ,  진용수 ,  이기성

IPC: C12N15/52 ,  C12N15/56 ,  C12N15/09

CPC classification number: C12N15/1034 ,  C07K14/395 ,  C12P7/04 ,  C12P7/06 ,  C12P7/16 ,  C12P7/28 ,  Y02E50/10 ,  Y02E50/17 ,  C12N15/52 ,  C12N9/24 ,  C12N15/09

Abstract: PURPOSE: An SNR84 gene enhancing usability of galactose metabolism is provided to enhance metabolic rate of galactose and enhance productivity of bioalcohol from carbon source. CONSTITUTION: An SNR84 gene enhances galactose metabolism rate. The gene is denoted by sequence number 1. A pRS424 recombinant vector contains the SNR84 gene of sequence number 1. A transformed recombinant microorganism is obtained using the recombinant vector. The recombinant microorganism overexpresses the SNR84 gene. The microorganism is yeast. The yeast is Saccharomyces sp., Pachysolen sp., Clavispora sp., Kluyveromyces sp., Debaryomyces sp., Schwanniomyces sp., Candida sp., Pichia sp., or Dekkera sp. The deposit number KCTC 11388 BP of the recombinant microorganism is CEN.PK2-1D/pRS424-SNR84.

Abstract translation: 目的：提供增强半乳糖代谢可用性的SNR84基因，以提高半乳糖的代谢率，并提高生物醇从碳源的生产力。 构成：SNR84基因增强半乳糖代谢率。 该基因由序列号1表示.PRS424重组载体含有序列号1的SNR84基因。使用重组载体获得转化的重组微生物。 重组微生物过表达SNR84基因。 微生物是酵母。 酵母是酵母属（Saccharomyces sp。），Pachysolen sp。，Clavispora sp。，Kluyveromyces sp。，Debaryomyces sp。，Schwanniomyces sp。，Candida sp。，Pichia sp。或Dekkera sp。 重组微生物的保藏号KCTC 11388 BP为CEN.PK2-1D / pRS424-SNR84。

公开(公告)号：KR1020130001121A

公开(公告)日：2013-01-03

申请号：KR1020120061819

申请日：2012-06-08

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 정순천 ,  구현민 ,  김재영 ,  김지은 ,  김진우 ,  박영경 ,  이소영 ,  조화영 ,  권대혁 ,  박재찬

IPC: C12N1/19 ,  C12N1/21 ,  C12N15/53 ,  C12P7/56

Abstract: PURPOSE: A modified microorganism for producing lactic acids and a method for producing lactic acids are provided to produce lactic acids with high efficiency under an acidic condition. CONSTITUTION: A modified microorganism for producing lactic acids with high efficiency has LDH activity of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, and Rattus norvegicus. The modified microorganism is yeast or bacteria. The modified microorganism is E.coli or Kluyveromyces marxianus. The modified microorganism produces lactic acids with 12.2% or more of glucose. An expression vector for producing the modified microorganism comprises: a replication origin; a promoter; a polynucleotide; and a terminator. A method for producing lactic acids comprises: a step of culturing the modified microorganism in a medium containing glucose; and a step of collecting lactic acids from the culture.

Abstract translation: 目的：提供用于生产乳酸的改性微生物和生产乳酸的方法，以在酸性条件下高效生产乳酸。 构成：用于高效生产乳酸的改良微生物具有日本芍药，鸟腥草，Tur ps属，and and属的LDH活性。 修饰的微生物是酵母或细菌。 修饰的微生物是大肠杆菌或马克斯克鲁维酵母（Kluyveromyces marxianus）。 经修饰的微生物产生具有12.2％或更多葡萄糖的乳酸。 用于产生修饰微生物的表达载体包括：复制起点; 启动子 多核苷酸; 和终结者。 制备乳酸的方法包括：在含有葡萄糖的培养基中培养经修饰的微生物的步骤; 以及从培养物中收集乳酸的步骤。

公开(公告)号：KR1020130001103A

公开(公告)日：2013-01-03

申请号：KR1020110139520

申请日：2011-12-21

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 정순천 ,  구현민 ,  김재영 ,  김지은 ,  김진우 ,  박영경 ,  이소영 ,  조화영 ,  권대혁 ,  박재찬

IPC: C12N15/63 ,  C12N1/21 ,  C12N15/53 ,  C12P7/56

Abstract: PURPOSE: A modified microorganism for producing lactic acids is provided to prepare lactic acids with high efficiency under an acidic condition. CONSTITUTION: A modified microorganism for producing lactic acids has a lactate dehydrogenase(LDH) activity of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, or Rattus norvegicus. The modified microorganism is Escherichia sp. or Kluyveromyces sp. The modified microorganism produces lactic acids with 34% or more of glucose. An expression vector contains: a replication origin for constructing the modified microroganisms; a promoter; a polynucleotide coding LDH activation of one or more species selected from the group consisting of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, or Rattus norvegicus; and a terminator. The replication origin is ARS/CEN replication origin.

Abstract translation: 目的：提供一种用于生产乳酸的改性微生物，用于在酸性条件下高效制备乳酸。 构成：用于生产乳酸的改良微生物具有日本芍药（Ornisorhynchus anatinus，Tursiops truncates）或褐藻（Rattus norvegicus）的乳酸脱氢酶（LDH）活性。 修饰的微生物是大肠杆菌属 或克鲁维酵母属 经修饰的微生物产生具有34％以上葡萄糖的乳酸。 表达载体包含：用于构建修饰的微结构的复制起点; 启动子 编码一种或多种选自以下的物质的LDH活化的多核苷酸：日本Pe us，鸟，us us us，，，;;;;;;;;;;;;;;;;;; 和终结者。 复制起点是ARS / CEN复制来源。

公开(公告)号：KR1020130000883A

公开(公告)日：2013-01-03

申请号：KR1020110061677

申请日：2011-06-24

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 구현민 ,  박성민 ,  유병조 ,  이기성 ,  박재찬 ,  권대혁

IPC: C12N15/70 ,  C12N15/81 ,  C12N15/67

CPC classification number: C12N15/815 ,  C12P21/00 ,  C12P21/02

Abstract: PURPOSE: An expression vector which overexpresses target proteins, and a method for producing the target protein are provided to obtain the proteins in K. marxianus. CONSTITUTION: An expression vector contains: a replication origin; a CYC promoter, a TEF promoter, a GPD promoter, or an ADH promoter; and a terminator. The CYC promoter contains a sequence of sequence number 1 or a sequence having 70% or more sequence homology with the sequence of sequence number 1. The TEF promoter has a sequence of sequence number 2 or a sequence with 70% or more sequence homology with the sequence of sequence number 2. GPD promoter contains a sequence of sequence number 3 or a sequence having 70% or more homology with the sequence of sequence number 3. The ADH promoter has a sequence of sequence number 4 or a sequence having 70% or more sequence homology with the sequence of sequence number 4.

Abstract translation: 目的：提供过表达靶蛋白的表达载体和产生靶蛋白的方法，以获得马克斯马氏霉属中的蛋白质。 构成：表达载体包含：复制起点; CYC启动子，TEF启动子，GPD启动子或ADH启动子; 和终结者。 CYC启动子含有序列号1的序列或与序列号1的序列具有70％以上序列同源性的序列.TEF启动子具有序列号2的序列或具有70％以上序列同源性的序列 序列号2的序列.GPD启动子含有序列号3的序列或与序列号3的序列具有70％以上同源性的序列.ADH启动子具有序列号4的序列或70％以上的序列 与序列号4的序列同源性。

公开(公告)号：KR1020100048224A

公开(公告)日：2010-05-11

申请号：KR1020080107277

申请日：2008-10-30

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 유병조 ,  박재찬 ,  구현민 ,  진용수 ,  이기성

IPC: C12N15/12 ,  C12N1/16 ,  C12N15/66

CPC classification number: C12P7/08 ,  C07K14/395 ,  Y02E50/17

Abstract: PURPOSE: A gene which enhances metabolic rate of galactose is provided to enhance productivity of bioalcohol from biomass. CONSTITUTION: A gene which enhances metabolic rate of galactose is formed by losing whole or partial expression suppressing domain in a regulatory gene which suppresses expression of galactose matobolic gene. The regulatory gene is a gene encoding a TUP1 protein. A domain suppressing expression of the gene is a C-terminal repressor domain. The regulatory gene has a polynucleotide sequence of sequence number 1. The TUP1 protein has an amino acid sequence of sequence number 2. A pRS424 recombinant vector contains the of sequence number 1. A transformed recombinant microorganism is prepared using the recombinant vector. The microorganism is yeast. The recombinant microorganism is Saccharomyces cerevisiae CEN.PK2-1D/pRS424-truncated TUP1 of deposit number KCTC 11387 BP.

Abstract translation: 目的：提高提高半乳糖代谢率的基因，以提高生物质中生物醇的生产力。 构成：通过在抑制半乳糖基质的表达的调节基因中失去全部或部分表达抑制结构域，形成提高半乳糖代谢率的基因。 调节基因是编码TUP1蛋白的基因。 抑制基因表达的结构域是C末端阻遏酶结构域。 调节基因具有序列号1的多核苷酸序列.TUP1蛋白具有序列号2的氨基酸序列.PRS424重组载体含有序列号1.使用重组载体制备转化的重组微生物。 微生物是酵母。 重组微生物是保藏号为KCTC 11387BP的酿酒酵母CEN.PK2-1D / pRS424-截短的TUP1。

公开(公告)号：KR1020140109183A

公开(公告)日：2014-09-15

申请号：KR1020130023575

申请日：2013-03-05

Applicant: 삼성전자주식회사

Inventor： 정순천 ,  구현민 ,  김지은 ,  박재찬 ,  박준성 ,  이주영

IPC: C12N1/19 ,  C12N1/15 ,  C12N5/10 ,  C12N15/63

CPC classification number: C12N15/80 ,  C12N15/81 ,  C12N15/815

Abstract: An embodiment of the present invention relates to a technology of multiple gene insertion by culturing a microorganism to be transformed to have 2.0 to 10.0 at OD600 and inserting a variety of genes into the microorganism at thee same time. According to the method, the variety of genes are introduced to the microorganism by one transformation. The present invention can be usefully used for industry because a transformed microorganism can easily be prepared.

Abstract translation: 本发明的一个实施方案涉及通过培养要转化的微生物在OD600处具有2.0至10.0并同时将多种基因插入到微生物中的多基因插入技术。 根据该方法，通过一次转化将多种基因导入微生物。 本发明可以有用地用于工业，因为可以容易地制备转化的微生物。

公开(公告)号：KR1020140043559A

公开(公告)日：2014-04-10

申请号：KR1020120105939

申请日：2012-09-24

Applicant: 삼성전자주식회사 ,  한국과학기술원

Inventor： 이규상 ,  김태용 ,  손승범 ,  구현민 ,  박재찬 ,  이상엽

IPC: C12N1/19 ,  C12N15/52 ,  C12N15/81 ,  C12P7/52

Abstract: An embodiment relates to a metabolic network model for analyzing metabolic characteristics of Kluyveromyces marxianus microorganisms for producing 3HP and to metabolic characteristic analysis of Kluyveromyces marxianus using the same. More specifically, the present invention relates to building metabolic network model of the Kluyveromyces marxianus using gene-protein-biochemistry reaction relationship, to analysis of metabolic characteristics of metabolic flux using the model, and to a method for predicting a novel metabolic path which enhances production capacity of the 3HP using simulation based on metabolic flux. The method of an embodiment can efficiently predict cell growth speed and production of microorganisms, save time and costs for optimizing the novel path, and provide variant microorganisms which can produce specific metabolites with high efficiency.

Abstract translation: 一个实施方案涉及用于分析用于产生3HP的马克斯克鲁维酵母微生物的代谢特征的代谢网络模型和使用其的马克斯克鲁维酵母的代谢特征分析。 更具体地说，本发明涉及使用基因 - 蛋白 - 生物化学反应关系构建马克斯克鲁维酵母的代谢网络模型，使用该模型分析代谢通量的代谢特征，以及用于预测增加生产的新型代谢途径的方法 使用基于代谢通量的模拟的3HP容量。 实施方案的方法可以有效地预测细胞生长速度和微生物的产生，从而节省了用于优化新途径的时间和成本，并提供可以高效生产特定代谢物的变体微生物。