Abstract:
PURPOSE: A method for producing cloned canidae is provided to conditionally express a specific gene using a Tet-on system. CONSTITUTION: A method for producing cloned canidae is as follows. A donor nucleus cell is prepared from a somatic cell collected from a canine animal. A DNA structure containing a target gene and rtTA2S-M2 trans activator sequence is introduced into the donor nucleus cell and is expressed. The donor nucleus cell with the target gene and rtTA2 S-M2 trans activator sequence is cultivated. The cultivated donor nucleus cell is transplanted to a recipient to prepare a nuclear transfer embryo and the nuclear transfer embryo is activated. The activated nuclear transfer embryo is transplanted to a surrogate mother.
Abstract:
PURPOSE: A composition containing bacteriocin cerein 13061 for killing germ is provided to replace antibiotics used in the food industry or livestock industry. CONSTITUTION: A polypeptide with a bacteriocin activity contains an amino acid sequence of sequence number 1 or 2. A polynucleotide encoding the amino acid of sequence number 1 has a nucleic acid sequence of sequence number 3. A polynucleotide encoding the amino acid of sequence number 2 has a nucleic acid sequence of sequence number 5. A composition for killing germ independently contains the polypeptide of sequence number 1 or 2 or a combination of the polypeptides. The composition contains a culture of Bacillus cereus ATCC 13061 as an active ingredient.