公开(公告)号：KR1020120116791A

公开(公告)日：2012-10-23

申请号：KR1020110034445

申请日：2011-04-13

Applicant: 서울대학교산학협력단

Inventor： 박태현 ,  홍승훈 ,  이상훈 ,  진혜준 ,  송현석 ,  박주훈

IPC: G01N27/414 ,  G01N27/327 ,  G01N27/416

Abstract: PURPOSE: A method for manufacturing a bio sensor platform in which an olfactory receptor protein is pattern-evaporated, the bio sensor platform manufactured by the same, a field-effect transistor including the same, and a bio sensor are provided to introduce a functional stage by chemically processing a surface of a patterned nano structure and to function as an olfactory receptor, thereby obtaining improved selectivity and sensitivity for detecting smell substances. CONSTITUTION: A method for manufacturing a bio sensor platform in which an olfactory receptor protein is pattern-evaporated is as follows. A substrate surface is patterned with photoresist. A self-assembled monolayer of polyethylene glycol is pattern-formed on the patterned substrate surface. The photoresist is eliminated from the substrate in which the self-assembled monolayer of the polyethylene glycol is formed. A nano structure is evaporated in a portion of the substrate in which the self-assembled monolayer of the polyethylene glycol is not formed so that the polyethylene glycol and nano structure manufacture a pattern-evaporated substrate. The nano structure evaporated in the substrate is chemically processed, thereby having an amine. A surface of the nano structure is activated. The olfactory receptor protein is fixed to the activated nano structure.

Abstract translation: 目的：提供一种生物传感器平台的制造方法，其中嗅觉受体蛋白被图案蒸发，由其制造的生物传感器平台，包括该生物传感器平台的场效应晶体管和生物传感器，以引入功能级 通过化学处理图案化纳米结构的表面并用作嗅觉受体，从而获得用于检测气味物质的改进的选择性和灵敏度。 构成：嗅觉受体蛋白质模式蒸发的生物传感器平台的制造方法如下。 衬底表面用光致抗蚀剂构图。 聚乙二醇的自组装单层图案形成在图案化的衬底表面上。 光致抗蚀剂从其中形成聚乙二醇的自组装单层的基材中除去。 在其中未形成聚乙二醇的自组装单层的部分基底中蒸发纳米结构，使得聚乙二醇和纳米结构制造图案蒸发的基底。 在衬底中蒸发的纳米结构被化学处理，从而具有胺。 纳米结构的表面被激活。 嗅觉受体蛋白质固定在活性纳米结构上。

公开(公告)号：KR101637530B1

公开(公告)日：2016-07-11

申请号：KR1020140170505

申请日：2014-12-02

Applicant: 서울대학교산학협력단

Inventor： 박태현 ,  홍승훈 ,  고휘진 ,  손만기 ,  조동국 ,  임종현 ,  박주훈

IPC: G01N27/414 ,  G01N33/18 ,  C07K14/435

Abstract: 본발명은후각수용체단백질을포함하는나노베시클이고정화된전계효과트랜지스터및 이를포함하는수질측정용바이오센서에관한것이다. 구체적으로, 본발명에따른수질측정용바이오센서는후각수용체단백질 hOR51S1 및 hOR3A4를포함하는나노베시클이고정화된전계효과트랜지스터를이용하여수질내의대표적인악취물질인 Geosmin과 2-메틸이소보르네올(2-Methylisoborneol)을높은민감도로실시간검지가능하다.

公开(公告)号：KR1020160066625A

公开(公告)日：2016-06-13

申请号：KR1020140170505

申请日：2014-12-02

Applicant: 서울대학교산학협력단

Inventor： 박태현 ,  홍승훈 ,  고휘진 ,  손만기 ,  조동국 ,  임종현 ,  박주훈

IPC: G01N27/414 ,  G01N33/18 ,  C07K14/435

CPC classification number: G01N27/414 ,  C07K14/435 ,  G01N33/18

Abstract: 본발명은후각수용체단백질을포함하는나노베시클이고정화된전계효과트랜지스터및 이를포함하는수질측정용바이오센서에관한것이다. 구체적으로, 본발명에따른수질측정용바이오센서는후각수용체단백질 hOR51S1 및 hOR3A4를포함하는나노베시클이고정화된전계효과트랜지스터를이용하여수질내의대표적인악취물질인 Geosmin과 2-메틸이소보르네올(2-Methylisoborneol)을높은민감도로실시간검지가능하다.

Abstract translation: 本发明涉及具有嗅觉受体蛋白质的纳米尺寸固定场效应晶体管和包含该嗅觉受体蛋白质的水质测量生物传感器。 更具体地，根据本发明，水质测量生物传感器使用包含hOR51S1嗅觉受体蛋白和hOR3A4嗅觉受体蛋白的纳米尺寸固定场效应晶体管，以实时检测高灵敏度的Geosmin和2-甲基异硼醇，其中 Geosmin和2-甲基异山梨醇是水中的代表性气味物质。

公开(公告)号：KR101489951B1

公开(公告)日：2015-02-06

申请号：KR1020130016062

申请日：2013-02-14

Applicant: 서울대학교산학협력단

Inventor： 홍승훈 ,  남좌민 ,  박은진 ,  박주훈 ,  이동준

IPC: G01N27/72 ,  C12Q1/68

CPC classification number: G01N27/745

Abstract: 본 발명은 미량의 타겟물질도 빠른 시간 내에 검출할 수 있는 바이오센서 및 이를 이용한 타겟물질 검출방법을 위하여, 수용체가 고정될 수 있으며 강자성체물질을 포함하는 수용체고정부와, 상기 수용체고정부에 방향이 가변하는 자기장을 인가할 수 있는 자기장인가부를 구비하는, 바이오센서 및 이를 이용한 타겟물질 검출방법을 제공한다.

公开(公告)号：KR1020140102568A

公开(公告)日：2014-08-22

申请号：KR1020130016062

申请日：2013-02-14

Applicant: 서울대학교산학협력단

Inventor： 홍승훈 ,  남좌민 ,  박은진 ,  박주훈 ,  이동준

IPC: G01N27/72 ,  C12Q1/68

CPC classification number: G01N27/745 ,  C12Q1/6834 ,  C12Q1/6837 ,  G01N27/72

Abstract: The present invention provides a biosensor and a method for detecting a target material using the same. The biosensor can quickly detect the target material, even in a small amounts. The biosensor includes the following: a receptor fixing part in which a receptor is fixed and a ferromagnetic substance is included; a magnetic field applying part which can apply an electric field with various directions to the receptor fixing part.

Abstract translation: 本发明提供一种生物传感器和使用其的目标材料的检测方法。 生物传感器即使少量也可快速检测目标物质。 生物传感器包括：固定受体的受体固定部和包含铁磁性物质的受体固定部; 可以将各种方向的电场施加到受体固定部的磁场施加部。

公开(公告)号：KR1020130084553A

公开(公告)日：2013-07-25

申请号：KR1020120005415

申请日：2012-01-17

Applicant: 서울대학교산학협력단

Inventor： 홍승훈 ,  남궁선 ,  박주훈

IPC: C01B31/02 ,  C12M3/00 ,  C12N11/02 ,  C12N5/07

CPC classification number: C01B32/168 ,  C01B2202/08 ,  C01B2202/20 ,  C12M3/00 ,  C12N5/06 ,  C12N11/02

Abstract: PURPOSE: A manufacturing method of a carbon nanotube substrate for regulating the growth and differentiation of cells is provided to regulate the growth and differentiation of Human mesenchymal stem cells (hMSCs) separated from bone marrow without a differentiation medium by spin-coating carbon nanotubes on a substrate in order to control the density of the carbon nanotubes on the substrate. CONSTITUTION: A manufacturing method of a carbon nanotube substrate for regulating the growth and differentiation of cells comprises the steps of: aligning carbon nanotubes on a substrate in one direction based on a spin-coating method; and coating poly (ethylene glycol) (PEG) on the substrate with aligned carbon nanotubes. The substrate is a glass cover coated with titanium (Ti) and gold (Au). The carbon nanotubes are single-walled carbon nanotubes (SWNT). The rotation speed of the spin-coating process is in the range of 4000-6000 rpm. During the carbon nanotube aligning step, the surface density of the aligned carbon nanotubes is controlled on the substrate in order to regulate the growth and differentiation of hMSCs. A method for regulating the growth and differentiation of hMSCs comprises a step of culturing hMSCs separated from bone marrow on a carbon nanotube substrate. [Reference numerals] (AA) Example 1; (BB) Comparative example 1

Abstract translation: 目的：提供一种用于调节细胞生长和分化的碳纳米管底物的制造方法，以通过在碳纳米管上旋转涂覆来调节从没有分化培养基的骨髓分离的人间充质干细胞（hMSCs）的生长和分化 底物，以便控制衬底上的碳纳米管的密度。 构成：用于调节细胞生长和分化的碳纳米管衬底的制造方法包括以下步骤：基于旋涂法在一个方向上将碳纳米管沿着一个方向排列; 并在具有排列的碳纳米管的基底上涂覆聚（乙二醇）（PEG）。 基材是涂有钛（Ti）和金（Au）的玻璃盖。 碳纳米管是单壁碳纳米管（SWNT）。 旋涂速度在4000-6000rpm的范围内。 在碳纳米管取向步骤期间，将对准的碳纳米管的表面密度控制在基底上，以调节hMSC的生长和分化。 调节hMSCs生长和分化的方法包括在碳纳米管底物上培养从骨髓分离的hMSCs的步骤。 （标号）（AA）实施例1; （BB）比较例1