Abstract:
본 발명은 인간 미각 수용체 기능화된 단일벽 탄소 나노튜브-전계효과 트랜지스터(swCNT-FET)에 기초하고 인간의 미각 시스템을 모사한 고선택성의 바이오 전자혀에 관한 것이다. 본 발명자들은 최초로 미각 수용체 단백질을 인공혀에 적용하였으며 고선택성, 고민감성의 전자혀를 성공적으로 증명하였다. 이는 인간의 미각 수용체 단백질을 전자혀의 1차 신호 변환기로 사용함으로써 인간의 미각 시스템을 모방하는 전자혀를 구현하였다. 또한 기존의 전자혀에 비해 선택성과 민감성 측면에서 매우 우수하다. 본 발명에 의한 바이오 전자혀는 식품 산업에서 품질검사의 목적이나 맛 물질의 선별을 위한 도구로 사용될 수 있다.
Abstract:
PURPOSE: A method for manufacturing a bio sensor platform in which an olfactory receptor protein is pattern-evaporated, the bio sensor platform manufactured by the same, a field-effect transistor including the same, and a bio sensor are provided to introduce a functional stage by chemically processing a surface of a patterned nano structure and to function as an olfactory receptor, thereby obtaining improved selectivity and sensitivity for detecting smell substances. CONSTITUTION: A method for manufacturing a bio sensor platform in which an olfactory receptor protein is pattern-evaporated is as follows. A substrate surface is patterned with photoresist. A self-assembled monolayer of polyethylene glycol is pattern-formed on the patterned substrate surface. The photoresist is eliminated from the substrate in which the self-assembled monolayer of the polyethylene glycol is formed. A nano structure is evaporated in a portion of the substrate in which the self-assembled monolayer of the polyethylene glycol is not formed so that the polyethylene glycol and nano structure manufacture a pattern-evaporated substrate. The nano structure evaporated in the substrate is chemically processed, thereby having an amine. A surface of the nano structure is activated. The olfactory receptor protein is fixed to the activated nano structure.
Abstract:
PURPOSE: A method for preparing nano vesicles containing olfactory receptor proteins is provided to ensure high selectivity and sensitivity. CONSTITUTION: A method for preparing nano vesicles containing olfactory receptor protein comprises: a step of transforming animal cells with a gene encoding the protein; a step of treating the animal cells with a compound to secrete nano vesicles containing the proteins; and a step of centrifuging the isolated nano vesicles. A transistor in which the nano vesicle is fixed comprises: a source electrode and a drain electrode separately formed on a substrate; a nanostructure which electrically contacts with the electrodes and is formed between the electrodes; and the protein on the surface of the electrodes and nanostructure.
Abstract:
본발명은이종이합체 G 단백질연결수용체를포함하는나노베지클, 이의제조방법및 이를포함하는전계효과트랜지스터기반미각센서, 이의제조방법에관한것이다. 본발명에따른이종이합체 G 단백질연결수용체를포함하는나노베지클로기능화된전계효과트랜지스터및 미각센서는이종이합체 G 단백질연결수용체및 이를포함하는나노베지클을이용하여, 민감도및 선택도가우수하고실시간으로고특이적으로단맛물질을검출할수 있다.
Abstract:
본 발명은 이종이합체 G 단백질 연결 수용체를 포함하는 나노베지클, 이의 제조 방법 및 이를 포함하는 전계효과 트랜지스터 기반 미각 센서, 이의 제조 방법에 관한 것이다. 본 발명에 따른 이종이합체 G 단백질 연결 수용체를 포함하는 나노베지클로 기능화된 전계효과 트랜지스터 및 미각 센서는 이종이합체 G 단백질 연결 수용체 및 이를 포함하는 나노베지클을 이용하여, 민감도 및 선택도가 우수하고 실시간으로 고특이적으로 단맛 물질을 검출할 수 있다.
Abstract:
The present invention comprises: a substrate; a semiconductor channel which is made of a single-walled carbon nanotube formed on the substrate; a single-walled carbon nanotube field effect transistor (swCNT-FET) including a source-drain electrode, which is formed in both ends of the semiconductor channel; and an acetylcholine receptor protein lipid membrane which is formed in at least part of a surface of the semiconductor channel. The acetylcholine receptor protein provides the swCNT-FET based acetylcholine sensor, which selectively binds an acetylcholine .
Abstract:
PURPOSE: A functionalization method of a single-walled carbon nanotube field-effect transistor (FET) by using a self-assembling peptide, a trimethylamine detection sensor containing a single-walled carbon nanotube FET manufactured by using the same, and a seafood freshness measuring method by using the sensor are provided to improve functions by combining a substance desired for detection with amino acid. CONSTITUTION: A semiconductor channel is constructed with single-walled carbon nanotubes. The single-walled carbon nanotubes are formed on a substrate. Source-drain electrodes are formed at both sides of the semiconductor channel. An amino acid cluster is combined with the terminus of a peptide. The peptide is self-assembled on the surface of the semiconductor channel.