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公开(公告)号：KR101844452B1
公开(公告)日：2018-05-18
申请号：KR1020170001890
申请日：2017-01-05
Applicant: 아주대학교산학협력단
IPC: C12N15/70 , C07K14/245 , C12P7/18 , C12P21/00
CPC classification number: C12N15/70 , C07K14/245 , C12N2511/00 , C12P7/18 , C12P21/00
Abstract: 본발명은용존산소의농도에따라서작동되는나르프로모터의개량에관한것으로, 더욱자세하게는나르프로모터의 -10과 -35 보존서열에무작위돌연변이를일으켜프로모터세기를개량한나르프로모터및 이를이용한바이오케미컬생산량증대에관한것이다. 본발명에따른 -10과 -35 보존서열에무작위돌연변이에의해프로모터세기가개량된나르프로모터는목적물질생산에관여하는대사경로단백질의발현이우수하므로, 상기단백질에의해활성화된대사경로를통해산업적으로유용한바이오케미컬의생산에효과적으로사용될수 있다.
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公开(公告)号：KR1020160148470A
公开(公告)日：2016-12-26
申请号：KR1020160074415
申请日：2016-06-15
Applicant: 아주대학교산학협력단
CPC classification number: C12N15/63 , C12N15/70 , C12N15/74 , C12N15/81 , C12P5/00 , C12P5/007 , C12Y205/0102 , C12Y205/01031
Abstract: 본발명은재조합미생물을이용한천연고무의생산방법을개시한다. 구체적으로본 발명은 (a) 서열번호 2의아미노산서열로특정되는민들레유래또는서열번호 6의아미노산서열로특정되는고무나무유래천연고무합성효소인시스-프레닐트랜스퍼라제를암호화하는유전자를발현시킬수 있는발현벡터와천연고무전구체합성효소를암호화하는유전자를발현시킬수 있는발현벡터를제작하는단계, (b) 상기발현벡터를숙주미생물에형질전환시키는단계, (c) 상기형질전환된숙주미생물을배양하는단계, 및 (d) 그배양된형질전환숙주미생물로부터천연고무를분리하는단계를포함하는재조합미생물을이용한천연고무의생산방법을개시한다. 본발명의방법에따라얻어진천연고무는천연고무와동일한백색의분말성상을가지고, 그 FT-IR 스펙트럼양상등이천연고무극히유사한양상을보인다.
Abstract translation: 公开了一种使用重组微生物生产天然橡胶的方法。 特别公开了通过使用重组微生物生产天然橡胶的方法，其包括以下步骤：（a）制备能够表达编码顺式 - 异戊烯基转移酶的基因的表达载体，所述基因是由 SEQ ID NO：2的氨基酸序列或由SEQ ID NO：6的氨基酸序列表示的来自巴西双雄草的天然橡胶合成酶，以及能够表达编码天然橡胶前体合成酶的基因的表达载体; （b）用表达载体转化宿主微生物; （c）培养转化的宿主微生物; 和（d）从培养的转化的宿主微生物中分离天然橡胶。 根据本发明的方法获得的天然橡胶具有与天然橡胶相同的白色粉末形式，并且显示出与天然橡胶非常相似的FT-IR光谱图案等。
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公开(公告)号：KR1020160083426A
公开(公告)日：2016-07-12
申请号：KR1020140194792
申请日：2014-12-31
Applicant: 아주대학교산학협력단
CPC classification number: C12N15/70 , C12N9/001 , C12N9/0073 , C12N9/1048 , C12N9/1085 , C12N9/90 , C12N15/01 , C12P23/00 , C12Y103/9903 , C12Y114/13129 , C12Y205/01001 , C12Y205/01032 , C12Y505/01019 , C12N9/0071
Abstract: 본발명은카로티노이드과생산능을갖는돌연변이주및 상기돌연변이주를이용한카로티노이드생산방법에관한것이다. 본발명에따른재조합벡터로형질전환된후, 방사선조사에의하여돌연변이가유발된돌연변이주는카로티노이드생산능이우수하고, 대량생산이가능하여, 카로티노이드를이용하는화장품, 식품, 사료등 다양한산업분야에서유용하게이용될수 있다.
Abstract translation: 本发明涉及具有过度生成类胡萝卜素的能力的突变菌株和使用该突变株产生类胡萝卜素的方法。 根据本发明用重组载体转化，然后通过辐射变异的突变菌株具有优异的产生类胡萝卜素的能力，并且可以批量生产，从而可用于各种工业领域如化妆品， 食品，饲料等使用类胡萝卜素。
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公开(公告)号：KR101533352B1
公开(公告)日：2015-07-06
申请号：KR1020140146190
申请日：2014-10-27
Applicant: 아주대학교산학협력단
CPC classification number: C12Y505/01013 , C12N9/0073 , C12N9/1085 , C12N9/88 , C12N9/90 , C12P5/007 , C12P7/42 , C12P15/00 , C12Y114/13078 , C12Y205/01029 , C12Y402/03019
Abstract: 본발명은스테비오사이드의전구체인카우린생성능을가지는재조합미생물및 이를이용한카우린의제조방법에관한것이다. 본발명에따르면스테비아의추출물로부터직접분리하는것이아닌, 미생물에도입된재조합유전자에의해대사경로를조작함으로써, 간단한공정만으로도천연감미료인스테비오사이드의전구체인카우린을다량으로제공할수 있다.
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公开(公告)号：KR1020140016654A
公开(公告)日：2014-02-10
申请号：KR1020120083513
申请日：2012-07-30
Applicant: 아주대학교산학협력단 , 삼성전자주식회사
CPC classification number: C12P7/18 , C12N9/0006 , C12N9/0008 , C12P7/24
Abstract: One embodiment confirms an ability to produce 1,4-butanediol as a result of being expressed within colon bacillus by introducing, from Clostridiumsaccharoperbutylacetonicum, butyraldehyde dehydrogenase (bld) and butanol dehydrogenase (bdh), which are enzymes activities of which have not been reported so far, for two reactions (4-hydroxybutyraldehyde producing reaction in 4-hydroxybutyryl-CoA and 1,4-butanediol producing reaction in 4-hydroxybutyraldehyde) at the end among 1,4-butanediol biosynthesis paths. In particular, the production concentration of 1,4-butanediol was enhanced by strengthening activities of the butyraldehyde dehydrogenase enzyme with a method of evolution in direction.
Abstract translation: 一个实施方案证实了通过从Clostridiumsaccharoperbutylacetonicum，丁醛脱氢酶（bld）和丁醇脱氢酶（bdh）引入的结肠杆菌中产生1,4-丁二醇的能力，其中酶活性尚未被报道 在1,4-丁二醇生物合成途径中的两个反应（4-羟基丁酰辅酶A中产生4-羟基丁醛产生反应和4-羟基丁醛中产生1,4-丁二醇的反应）。 特别地，1,4-丁二醇的生产浓度通过以方向发展的方法强化丁醛脱氢酶的活性来提高。
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Patent Agency Ranking
公开(公告)号：KR1020120124287A
公开(公告)日：2012-11-13
申请号：KR1020110042098
申请日：2011-05-03
Applicant: 아주대학교산학협력단
Abstract: PURPOSE: A biosynthesis method of staphyloxanthin-like carotenoids of a novel structure is provided to obtain novel staphyloxanthin-like substances and to produce a large amount of useful substances. CONSTITUTION: A gene derived from Staphylococcus aureus has a base sequence of sequence number 1. A recombinant vector contains the gene of sequence number 1 and also contains CrtM(diapophytoene synthase) gene(sequence number 2), CrtN(diapophytoene desaturase) gene(sequence number 3), CrtP(diaponeurosporene oxidase) gene(sequence number 4), CrtQ(glycosyl transferase) gene(sequence number 5), or CrtO(acyl transferase) gene(sequence number 6). E.coli which is transformed with the recombinant vector produces carotenoid.
Abstract translation: 目的：提供一种新型结构的葡萄球菌样类胡萝卜素的生物合成方法，以获得新型葡萄球菌样物质并产生大量有用物质。 构成：来自金黄色葡萄球菌的基因具有序列号1的碱基序列。重组载体含有序列号1的基因，并且还含有CrtM（二倍体植物油合酶）基因（序列号2），CrtN（嗜睡蛾类脱氢酶）基因（序列 3号），CrtP（diaponeurosporene oxidase）基因（序列号4），CrtQ（糖基转移酶）基因（序列号5）或CrtO（酰基转移酶）基因（序列号6）。 用重组载体转化的大肠杆菌产生类胡萝卜素。
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公开(公告)号：KR101849238B1
公开(公告)日：2018-06-01
申请号：KR1020160074415
申请日：2016-06-15
Applicant: 아주대학교산학협력단
Abstract: 본발명은재조합미생물을이용한천연고무의생산방법을개시한다. 구체적으로본 발명은 (a) 서열번호 2의아미노산서열로특정되는민들레유래또는서열번호 6의아미노산서열로특정되는고무나무유래천연고무합성효소인시스-프레닐트랜스퍼라제를암호화하는유전자를발현시킬수 있는발현벡터와천연고무전구체합성효소를암호화하는유전자를발현시킬수 있는발현벡터를제작하는단계, (b) 상기발현벡터를숙주미생물에형질전환시키는단계, (c) 상기형질전환된숙주미생물을배양하는단계, 및 (d) 그배양된형질전환숙주미생물로부터천연고무를분리하는단계를포함하는재조합미생물을이용한천연고무의생산방법을개시한다. 본발명의방법에따라얻어진천연고무는천연고무와동일한백색의분말성상을가지고, 그 FT-IR 스펙트럼양상등이천연고무극히유사한양상을보인다.
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