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公开(公告)号：WO2023085605A1
公开(公告)日：2023-05-19
申请号：PCT/KR2022/014746
申请日：2022-09-30
Applicant: 삼성전자주식회사
Abstract: 공기조화기의 실외기는, 밴딩되어 연장되는 열교환기와, 열교환기를 지지하도록 열교환기의 하방에 마련되고 열교환기로부터 흐르는 응축수를 수집하도록 배수로가 형성된 베이스와 열교환기를 지지하도록 배수로의 상방에 배치되는 지지부재로서, 일단이 베이스와 분리 가능하게 결합되고, 배수로의 일부를 커버하도록 연장되어 열교환기를 지지하는 지지판과 열교환기의 외측을 지지하도록 지지판의 타단으로부터 상방으로 연장되는 외판과 외판의 상단으로부터 경사지는 형상의 가이드판을 포함하는 지지부재를 포함할 수 있다.
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2.发明公开
公开(公告)号：KR1020150039055A
公开(公告)日：2015-04-09
申请号：KR1020130117595
申请日：2013-10-01
Applicant: 삼성전자주식회사 , 아주대학교산학협력단
CPC classification number: C12N9/0006 , C12N9/0008 , C12N9/13 , C12N15/70 , C12P7/18 , C12P7/24 , C12Y101/01061 , C12Y102/01057 , C12Y102/01076 , C12Y208/03
Abstract: 일구체예는 1,4-부탄다이올생합성경로중 말단의 2개반응인 4-hydroxybutyryl-CoA에서 4-히드록시부틸알데히드생성반응및 4-히드록시부틸알데히드에서 1,4-BDO 생성반응에대해현재까지활성이보고되지않은효소인부틸알데히드디히드로게나제와부탄올디히드로게나제를클로스트리디움사카로퍼부틸아세토니쿰으로부터도입하여대장균에서발현시킨결과 1,4-BDO 생산능이있음을확인하였다. 특히부틸알데히드디히드로게나제효소를방향진화방법으로활성를강화하여 1,4-BDO의생산농도를 2배이상향상시켰다.
Abstract translation: 根据本发明的一个实施方案，由Clostridiumsaccharoperbutylacetonicum，丁醛脱氢酶和丁醇脱氢酶引入1,4-BDO的生产率得到确认，该酶是这样的酶，其中包括4-羟基丁醛生成反应的两个反应的活性在4 在1,4-丁二醇生物合成途径末端的4-羟基丁醛中产生的4-羟基丁酰辅酶A和1,4-BDO产生反应迄今尚未被报道在大肠杆菌中表达。 特别地，通过定向进化法强化丁醛脱氢酶的活性，1,4-BDO的生产浓度提高了两倍以上。
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公开(公告)号：KR1020140016654A
公开(公告)日：2014-02-10
申请号：KR1020120083513
申请日：2012-07-30
Applicant: 아주대학교산학협력단 , 삼성전자주식회사
CPC classification number: C12P7/18 , C12N9/0006 , C12N9/0008 , C12P7/24
Abstract: One embodiment confirms an ability to produce 1,4-butanediol as a result of being expressed within colon bacillus by introducing, from Clostridiumsaccharoperbutylacetonicum, butyraldehyde dehydrogenase (bld) and butanol dehydrogenase (bdh), which are enzymes activities of which have not been reported so far, for two reactions (4-hydroxybutyraldehyde producing reaction in 4-hydroxybutyryl-CoA and 1,4-butanediol producing reaction in 4-hydroxybutyraldehyde) at the end among 1,4-butanediol biosynthesis paths. In particular, the production concentration of 1,4-butanediol was enhanced by strengthening activities of the butyraldehyde dehydrogenase enzyme with a method of evolution in direction.
Abstract translation: 一个实施方案证实了通过从Clostridiumsaccharoperbutylacetonicum，丁醛脱氢酶（bld）和丁醇脱氢酶（bdh）引入的结肠杆菌中产生1,4-丁二醇的能力，其中酶活性尚未被报道 在1,4-丁二醇生物合成途径中的两个反应（4-羟基丁酰辅酶A中产生4-羟基丁醛产生反应和4-羟基丁醛中产生1,4-丁二醇的反应）。 特别地，1,4-丁二醇的生产浓度通过以方向发展的方法强化丁醛脱氢酶的活性来提高。
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公开(公告)号：KR1020140122606A
公开(公告)日：2014-10-20
申请号：KR1020130039509
申请日：2013-04-10
Applicant: 삼성전자주식회사
Abstract: 일 양상에 따른 숙시닐-코에이:아세틸 코에이 트랜스퍼라제 또는 이의 변이체를 코딩하는 폴리뉴클레오티드, 3-옥소아디필-코에이 트랜스퍼라제 또는 이의 변이체를 코딩하는 폴리뉴클레오티드, ADH6 단백질 또는 이의 변이체를 코딩하는 폴리뉴클레오티드, BDHA 단백질 또는 이의 변이체를 코딩하는 폴리뉴클레오티드, 4HBD 단백질 또는 이의 변이체를 코딩하는 폴리뉴클레오티드 및 ALD5 단백질 또는 이의 변이체를 코딩하는 폴리뉴클레오티드를 포함하는 미생물은 외래 유전자의 도입으로 새로운 대사 경로를 갖게 되어 아디페이트를 효과적으로 생산할 수 있게 되었다. 이러한 형질전환된 미생물은 화학합성에 비하여 효율적으로 아디페이트를 생산할 수 있으므로 산업적으로 유용하게 이용할 수 있다.
Abstract translation: 根据本发明的一个实施方案，包括编码琥珀酰-CoA：乙酰辅酶A转移酶或其突变体的多核苷酸的微生物，编码3-氧基己二醇-CoA转移酶或其突变体的多核苷酸，编码ADH6蛋白或其突变体的多核苷酸，编码BDHA蛋白质的多核苷酸 或其突变体，编码4HD蛋白或其突变体的多核苷酸和编码ALD5蛋白或其突变体的多核苷酸可以通过引入外源基因具有新的代谢途径来有效地产生己二酸。 与化学合成相比，这种转化的微生物可以有效地产生己二酸酯，因此可以在工业上有用地使用。
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Patent Agency Ranking
公开(公告)号：KR1020140066553A
公开(公告)日：2014-06-02
申请号：KR1020120133940
申请日：2012-11-23
Applicant: 삼성전자주식회사
Abstract: Provided are a novel promoter of Corynebacterium glutamicum; a vector including the promoter; a host cell having the vector; and a method to express gene using the host cell. When comparing and analyzing the expression of the promoter with a conventional gapA promoter, high expression level is maintained not only under an aerobic condition but an anaerobic condition. Construction of a system which can overexpress desired genes in the Corynebacterium glutamicum using the promoter can be possible.
Abstract translation: 提供谷氨酸棒杆菌的新型启动子; 包含启动子的载体; 具有载体的宿主细胞; 以及使用宿主细胞表达基因的方法。 当比较和分析具有常规gapA启动子的启动子的表达时，不仅在需氧条件下而且在厌氧条件下保持高表达水平。 可以使用启动子构建能够过量表达谷氨酸棒杆菌中所需基因的系统。
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公开(公告)号：KR1020130094115A
公开(公告)日：2013-08-23
申请号：KR1020120015526
申请日：2012-02-15
Applicant: 삼성전자주식회사
CPC classification number: C12P7/18 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/1217 , C12N9/13 , C12N9/88 , C12Y101/01001 , C12Y101/01016 , C12Y102/01016 , C12Y102/04002 , C12Y203/01019 , C12Y207/02007 , C12Y208/03005 , C12Y401/01071
Abstract: PURPOSE: A deformed microorganism for production of 1,4-butanediol is provided to produce 1,4-BDO by a biological producing process. CONSTITUTION: A deformed microorganism for production of 1.4-butanediol comprises: an activation converting alpha-ketoglutarate or succinate into 4-hydroxybutyril-CoA ("4-HB-CoA"); and an activation converting the 4-HB-CoA into 1,4-butanediols ("1,4-BDO"). The activation converting the 4-HB-CoA into the 1,4-BDO is coded by more than one kind of genes selected from a group comprising adh1, yiaY, adh4, adhB, mdh, eutG, fucO, dhaT, aldA, eutE, adhE1, adhE2 and adh2.
Abstract translation: 目的：提供用于生产1,4-丁二醇的变形微生物，以通过生物制备方法生产1,4-BDO。 构成：用于生产1,4-丁二醇的变形的微生物包括：将α-酮戊二酸或琥珀酸转化成4-羟基丁酰辅酶A（“4-HB-CoA”）的活化; 和将4-HB-CoA转化为1,4-丁二醇（“1,4-BDO”）的活化。 将4-HB-CoA转化为1,4-BDO的激活由选自包含adh1，yyY，adh4，adhB，mdh，eutG，fucO，dhaT，aldA，eutE， adhE1，adhE2和adh2。
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公开(公告)号：KR1020130089213A
公开(公告)日：2013-08-09
申请号：KR1020130011992
申请日：2013-02-01
Applicant: 삼성전자주식회사
CPC classification number: A61K9/5169 , A61K9/5123 , A61K41/0028
Abstract: PURPOSE: A solid lipid nanoparticle is provided to offer an effective method for transferring an active ingredient to the target site of body. CONSTITUTION: A solid lipid nanoparticle comprises an elastin-like polypeptide in which the moiety having more than one hydrophobic groups are bound and lipid molecule. The moiety having hydrophobic group is the saturated or unsaturated hydrocarbon group, the saturated or unsaturated acyl group, the substitute amide group having a chemical formula -C(CO)N(R1)(R2), which means the R1 and R2 are the respectively independent saturated or unsaturated hydrocarbon, or the saturated or unsaturated alkoxy group. The lipid molecule is neutral lipid molecule or amphiphilic lipid molecule. The elastic-like polypeptide includes more than one repeat unit selected from among VPGXG, PGXGV, GXGVP, XGVPG, GVPGX, and a group comprised of combination thoseof more than once. The V, P, G, and X is respectively means valine, proline, glycine, and an amino acid which is not proline. [Reference numerals] (AA) Coefficient
Abstract translation: 目的：提供固体脂质纳米颗粒，以提供将活性成分转移到身体目标部位的有效方法。 构成：固体脂质纳米颗粒包含弹性蛋白样多肽，其中具有多于一个疏水基团的部分被结合，脂质分子。 具有疏水基团的部分是饱和或不饱和的烃基，饱和或不饱和的酰基，具有化学式-C（CO）N（R1）（R2）的取代酰胺基，这意味着R1和R2分别为 独立的饱和或不饱和烃，或饱和或不饱和的烷氧基。 脂质分子是中性脂质分子或两亲性脂质分子。 弹性状多肽包括多于一个选自VPGXG，PGXGV，GXGVP，XGVPG，GVPGX的重复单元，以及由多于一种的组合组成的组。 V，P，G和X分别表示缬氨酸，脯氨酸，甘氨酸和不是脯氨酸的氨基酸。 （标号）（AA）系数
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公开(公告)号：KR1020130000688A
公开(公告)日：2013-01-03
申请号：KR1020110061366
申请日：2011-06-23
Applicant: 삼성전자주식회사
Abstract: PURPOSE: A high throughput screening system and a method of lactic acid-producing strains using two or more pH indicators are provided to quickly and accurately screen strains. CONSTITUTION: A high throughput screening system of lactic acid-producing strains contains the strain, a medium, and two or more pH indicators. The strains are a wild type strain, a mutant strain, and a recombinant strain. The pH indicators contain bromocresol green and methyl red in a ratio of 2:1-10:1. A method for screening the lactic acid-producing strains comprises: a step of adding the pH indicators to a medium; a step of culturing the strains; and a step of observing color change of the pH indicators, and measuring lactic acid production quantity.
Abstract translation: 目的：提供高通量筛选系统和使用两种或多种pH指示剂的乳酸生产菌株的方法，以快速准确筛选菌株。 构成：乳酸生产菌株的高通量筛选系统含有菌株，培养基和两种或多种pH指示剂。 菌株是野生型菌株，突变菌株和重组菌株。 pH指标含有溴甲酚绿和甲基红，比例为2：1-10：1。 筛选乳酸生产菌株的方法包括：将pH指示剂加入到培养基中的步骤; 培养菌株的一个步骤; 以及观察pH指示剂的变色，测定乳酸生成量的工序。
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公开(公告)号：KR1020120099315A
公开(公告)日：2012-09-10
申请号：KR1020110007877
申请日：2011-01-26
Applicant: 삼성전자주식회사
CPC classification number: C12Y402/0103 , C12N9/0006 , C12N9/0008 , C12N9/88 , C12P7/18 , C12P7/42 , C12Y101/01202 , C12Y102/01
Abstract: PURPOSE: A method for preparing 3-HP(3-hydroxypropionic acid) and 1,3-PDO(1,3-propandiol) using microorganisms is provided to convert a glycerol substrate into 3-HP and 1,3-PDO. CONSTITUTION: A recombinant microorganism for preparing 3-HP and 1,3-PDO contains genes producing the compounds. The genes are dhaB(glycerol dehydratease), alhH(aldehyde dehydrogenase), and dhaT(1,3-propandiol oxidoreductase). dhaB gene is derived from K. pneumoniae or C. butyricum. alhH gene is derived from E.coli. The recombinant microorganism is E.coli. A method for producing 3-HP and 1,3-PDO comprises a step of culturing the recombinant microorganism in a medium containing a carbon substrate. The carbon substrate is glucose, sucrose, cellulose, or glycerol.
Abstract translation: 目的：提供使用微生物制备3-HP（3-羟基丙酸）和1,3-PDO（1,3-丙二醇）的方法，以将甘油底物转化为3-HP和1,3-PDO。 构成：用于制备3-HP和1,3-PDO的重组微生物含有产生该化合物的基因。 基因是dhaB（甘油脱水酶），alhH（醛脱氢酶）和dhaT（1,3-丙二醇氧化还原酶）。 dhaB基因衍生自肺炎克雷伯杆菌或C.丁酸菌。 alhH基因来自大肠杆菌。 重组微生物是大肠杆菌。 制备3-HP和1,3-PDO的方法包括在含有碳底物的培养基中培养重组微生物的步骤。 碳底物是葡萄糖，蔗糖，纤维素或甘油。
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