Abstract:
PURPOSE: A method for enhancing transformation efficiency using useful gene-introduced Agrobacterium is provided to stably transfer the gene into Chrysanthemum. CONSTITUTION: A method for enhancing transformation efficiency in Chrysanthemum comprises: a step of inoculating useful gene-introduced Agrobacterium with 0.7-1.0 of OD 600 value into a scratched leaf fragment of Chrysanthemum; a step of culturing the leaf fragment in a co-culture medium containing 20 mg/L of acetosyringone for 4 days; and a step of selecting the leaf fragments in a redifferentaition selective medium. The useful gene is a combination of CuZnSOD(copper zinc superoxide dismutase) gene and APX(ascorbate peroxidase) or NDPK2(nucleoside diphosphate kinase 2) gene.
Abstract:
PURPOSE: A composition containing yacon extract is provided to ensure strong antibacterial activity against bacteria causing dental caries or periodontitis. CONSTITUTION: An antibacterial composition against bacteria against dental caries or periodontitis contains yacon extract as an active ingredient. The bacteria causing dental caries are Streptococcus mutans, Streptococcus sobrinus, or Lactobacillus salivarius. A pharmaceutical composition for preventing or treating dental caries or periodontal diseases contains yacon extract as an active ingredient.