公开(公告)号：KR102092175B1

公开(公告)日：2020-03-23

申请号：KR1020190125766

申请日：2019-10-11

Applicant: 충남대학교산학협력단 ,  전남대학교산학협력단

Inventor： 박연일 ,  전진 ,  김정일

IPC: C12N15/82 ,  C07K14/415

公开(公告)号：KR101986770B1

公开(公告)日：2019-06-07

申请号：KR1020170109197

申请日：2017-08-29

Applicant: 충남대학교산학협력단

Inventor： 박연일

IPC: C07K14/415 ,  A61K8/64 ,  A61Q19/00

公开(公告)号：KR101494208B1

公开(公告)日：2015-03-09

申请号：KR1020130056766

申请日：2013-05-20

Applicant: 한국표준과학연구원 ,  충남대학교산학협력단

Inventor： 정세채 ,  박연일 ,  조성미 ,  송지영

IPC: A61K8/49 ,  A61K8/97 ,  A61K8/64 ,  A61Q17/04

CPC classification number: A61K8/49 ,  A61K8/64 ,  A61K8/97 ,  A61K2800/438 ,  A61Q17/04

Abstract: 본 발명에 따른 자외선 차단제 조성물은 자외선(UV A)에 노출 시 자외선을 흡수하여 가시광선 또는 근적외선(IR-A)의 흡수밴드를 나타내는 가시광선 및 근적외선 광수용체 (Z-form)로 변환되고, 상기 변환된 가시광선 및 근적외선 광수용체가 가시광선 또는 근적외선에 노출 시 가시광선 또는 근적외선을 흡수하여 자외선 영역의 흡수밴드를 나타내는 자외선 광수용체 (E-form)로 변환되는 광호변성 물질을 포함하는 자외선 차단용 조성물이다.

公开(公告)号：KR1020140090385A

公开(公告)日：2014-07-17

申请号：KR1020130002313

申请日：2013-01-09

Applicant: 한국생명공학연구원 ,  한국해양과학기술원 ,  충남대학교산학협력단

Inventor： 정원중 ,  안준우 ,  유장렬 ,  최한구 ,  박연일

IPC: C12N1/12 ,  C12P7/64 ,  C10L1/04 ,  C10G3/00

CPC classification number: C10L1/026 ,  C12P7/20 ,  C12P7/6463 ,  C12P7/649 ,  C12P19/04 ,  C12R1/89 ,  Y02E50/13 ,  Y02P30/20 ,  C12N1/12 ,  C10G3/00 ,  C10L1/04 ,  C12P7/64

Abstract: The present invention relates to new microalgae chlorella (Chlorella sp.) for accumulating high concentration functional starch and lipid. According to the present invention, a chlorella ArM29B cell line is confirmed as a cell line for accumulating high concentration starch and lipid in culturing, and cultured in various temperature conditions. Moreover, a nile red analysis method capable of specifically dyeing a neutral oil drop inside a cell confirms that high concentration lipid is accumulated. Therefore, the cell line can be used as a material of producing functional lipid and biodiesel. The chlorella ArM29B cell line accumulates the high concentration lipid in culturing, so the cell line is suitable for microalgae for biodiesel. All chlorella ArM29B cell lines are growing well above a freezing temperature, so there is no necessity for a specific temperature condition in culturing. All chlorella ArM29B cell lines are growing well in four seasons, so the cell line is produced and cultured throughout the year.

Abstract translation: 本发明涉及用于积聚高浓度功能性淀粉和脂质的新型微藻小球藻（小球藻）。 根据本发明，确认了小球藻ArM29B细胞系作为培养中积聚高浓度淀粉和脂质的细胞系，并在各种温度条件下培养。 此外，能够特异性染色细胞内的中性油滴的尼罗红分析方法证实高浓度脂质被积聚。 因此，细胞系可以用作生产功能脂质和生物柴油的材料。 小球藻ArM29B细胞系培养高浓度脂质，细胞系适用于生物柴油微藻。 所有小球藻ArM29B细胞系都在高于冰冻温度的条件下生长，因此培养时不需要特定的温度条件。 所有小球藻ArM29B细胞系在四个季节都有良好的生长，所以细胞系全年生产和培养。

公开(公告)号：KR1020110125576A

公开(公告)日：2011-11-21

申请号：KR1020100109292

申请日：2010-11-04

Applicant: 한국생명공학연구원 ,  충남대학교산학협력단 ,  한국해양과학기술원

Inventor： 정원중 ,  박연일 ,  최한구 ,  강성호 ,  김석원 ,  손수현 ,  유장렬

IPC: C12N1/12 ,  C12P7/64 ,  C12R1/89

Abstract: PURPOSE: A novel microalgae Chlamydomonas ArM29 is provided to accumulate lipid of high concentration. CONSTITUTION: A Chlamydomonas sp. strain is derived from arctic ocean and produces lipid of high concentration. The strain is Chlamydomonas sp. ArM29(KCTC 11695BP). The strain accumulates lipid of high concentration in a lipid body form. The temperature of culturing the strain is 0°C-30°C. A microorganism formulation for producing lipid contains the strain or culture liquid thereof as an active ingredient.

Abstract translation: 目的：提供一种新型微藻衣藻ArM29来积聚高浓度脂质。 构成：衣藻属 菌株来自北极洋，产生高浓度的脂质。 菌株是衣藻属 ArM29（KCTC 11695BP）。 该菌株以脂质体形式积累高浓度的脂质。 培养菌株的温度为0℃〜30℃。 用于生产脂质的微生物制剂含有作为活性成分的菌株或培养液。

公开(公告)号：KR1020090131309A

公开(公告)日：2009-12-29

申请号：KR1020080057115

申请日：2008-06-18

Applicant: 충남대학교산학협력단

Inventor： 박연일 ,  김수연

IPC: C12N15/52 ,  C12N15/09 ,  C12N15/64

Abstract: PURPOSE: An svr1(synechocystis 4-vinyl reductase 1) is provided to control cell size of cyanobacteria and research cell size regulation of other bacteria. CONSTITUTION: An svr1(synechocystis 4-vinyl reductase 1) regulates cell size of Synechocystis sp. The svr1 gene comprises a sequence of sequence numbr 1. A host cells are transformed using a recombinant vector containing svr1 gene. The host cell is Synechocystis sp. PCC6803. The cell size of the Synechocystis sp. is regulated by expressing svr1 gene. When the svr1 gene is overexpressed, the cell size of Synechocystis sp. is reduced. When the expression of svr1 is suppressed, the cell size of Synechocystis sp. is increased.

Abstract translation: 目的：提供svr1（synechocystis 4-乙烯基还原酶1）以控制蓝细菌的细胞大小，并研究其他细菌的细胞大小调节。 构成：svr1（synechocystis 4-乙烯基还原酶1）调节集胞藻的细胞大小。 svr1基因包含序列号1的序列。使用含有svr1基因的重组载体转化宿主细胞。 宿主细胞是集胞藻（Synechocystis sp。 6803。 集胞藻的细胞大小 通过表达svr1基因来调控。 当svr1基因过表达时，集胞藻的细胞大小 降低了。 当svr1的表达被抑制时，集胞藻的细胞大小 增加了。

公开(公告)号：KR1020070121088A

公开(公告)日：2007-12-27

申请号：KR1020060055689

申请日：2006-06-21

Applicant: 충남대학교산학협력단

Inventor： 김수연 ,  류지연 ,  최도일 ,  박연일

IPC: C12Q1/68 ,  G01N33/53

CPC classification number: C12Q1/6897 ,  C12Q1/6825 ,  G01N33/5308

Abstract: A biosensor is provided to be used for screening nutritive materials such as glucose, heavy metals or cyanogen or be applied to biopsy of herbicides or antibiotics, thereby being usefully employed to develop new drugs, detect environment-toxic materials of aquatic ecosystem, and screen residual pesticides and heavy metals at real-time in a non-destructive way. A glucose inducible biosensor comprises a microorganism including a promoter portion of a glucose inducible gene derived from Synechocystis sp. PCC6803, which is selected from the group consisting of atp I, ndbA, catD1, tkt, pgi, pdh, ppc and cydA. To screen glucose, environment-toxic materials, inhibitors of transcription or translation or harmful materials having the concentration over reference value, the biosensor is used.

Abstract translation: 提供生物传感器用于筛选葡萄糖，重金属或氰的营养物质，或用于除草剂或抗生素的活检，从而有效地用于开发新药，检测水生生态系统的环境毒性物质，筛选残留物 农药和重金属以非破坏性的方式实时传播。 葡萄糖诱导型生物传感器包括微生物，其包含源自集胞藻（Synechocystis sp。）的葡萄糖诱导型基因的启动子部分。 PCC6803，其选自atp I，ndbA，catD1，tkt，pgi，pdh，ppc和cydA。 为了筛选葡萄糖，环境毒性物质，转录或翻译抑制剂或浓度超过参考值的有害物质，使用生物传感器。

公开(公告)号：KR101669484B1

公开(公告)日：2016-10-26

申请号：KR1020160127368

申请日：2016-10-04

Applicant: 충남대학교산학협력단

Inventor： 박연일

IPC: C07K14/415 ,  C12N15/82

CPC classification number: C07K14/415 ,  C12N15/8271

Abstract: 본발명은서열번호 2의아미노산서열로이루어진, 톨리포스릭스(Tolypothrix) PCC 7910 유래의자색/오렌지색광호변성피토크롬 TpR0787 단백질, 상기단백질을코딩하는유전자, 상기유전자를포함하는재조합벡터및 서열번호 2의아미노산서열로이루어진 TpR0787 단백질의 258번째아미노산이시스테인에서세린으로돌연변이된녹색/적색광호변성피토크롬 TpR0787 C258S 변이체단백질에관한것으로, 본발명의 TpR0787 단백질은오렌지색과녹색을생장및 분화의정보원으로이용하지못하는조류와고등식물등의광합성식물에도입하면광형태형성과열형태형성을제어할수 있고, 식물체에음지내성을부여할수 있으며, 아파트형식물공장과온난화환경에서맞춤형작물의재배와생산성제고에기여할수 있는있는방법을제공할수 있을뿐만아니라광의학, 생체이미징, 세포학, 바이오에너지등 여러생명공학분야에다양하게활용될수 있을것이다.

公开(公告)号：KR101500952B1

公开(公告)日：2015-03-11

申请号：KR1020140042165

申请日：2014-04-09

Applicant: 충남대학교산학협력단

Inventor： 박연일 ,  조성미

IPC: C07K14/195 ,  C12N15/31 ,  A61K8/64

Abstract: 본 발명은 마이크로콜레우스 종 (
Microcoleus sp.) 유래의 광수용체 기능을 가지는 GAF (c
G MP-specific phosphodiesterase,
a denylyl cyclase and
F hlA) 도메인 단백질 및 상기 단백질을 코딩하는 유전자, 마이크로콜레우스 종 유래의 자외선/녹색 광호변성 색소 UGS (UV/Green light photoreversible Sensor) 단백질 및 상기 단백질을 코딩하는 유전자, 상기 유전자를 포함하는 재조합 벡터, 상기 재조합 벡터로 형질전환된 숙주세포, 상기 재조합 벡터를 숙주세포에 형질전환시켜 GAF 도메인 또는 UGS 단백질을 생산하는 방법, 상기 방법에 의해 생산된 GAF 도메인 또는 UGS 단백질 및 GAF 도메인 또는 UGS 단백질을 유효성분으로 포함하는 자외선 차단용 화장료 조성물에 관한 것이다.

Abstract translation: 本发明涉及具有衍生自Microcoleus sp的感光器功能的cGMP特异性磷酸二酯酶，腺苷酸环化酶和FhlA（GAF）结构域蛋白。 和编码该蛋白质的基因; 来自Microcoleus sp。的UV /绿光光可逆传感器（UGS）蛋白质 和编码该蛋白质的基因; 包含该基因的重组载体; 用重组载体转化的宿主细胞; 通过用重组载体转化宿主细胞来产生GAF结构域或UGS蛋白的方法; 以及含有通过该方法生产的GAF结构域或UGS蛋白质作为有效成分的UV阻断化妆品组合物。 根据本发明，预计光合作用效率将增加。

公开(公告)号：KR101337243B1

公开(公告)日：2013-12-05

申请号：KR1020120069628

申请日：2012-06-28

Applicant: 충남대학교산학협력단

Inventor： 박연일 ,  신동호

IPC: A01H5/00 ,  C12N15/29 ,  C12N15/82

CPC classification number: C12N15/825 ,  A01H4/008 ,  C07K14/415 ,  C12N15/8205 ,  C12N15/8222 ,  C12N15/8237

Abstract: The present invention relates to the following: a method of regulating the anthocyan biosynthesis of a plant body including a step of controlling the expression of a gene coding Arabidopsisthaliana derived AtPTR2 protein in a plant; a production method of a transformed plant body with the regulated anthocyan biosynthesis including a step of controlling the expression of the gene coding the AtPTR2 protein by transforming a recombination vector including the gene coding the the AtPTR2 protein on the plant body; and a composition for regulating the anthocyan biosynthesis of the plant body including the gene coding the the AtPTR2 protein, the transformed plant body with the regulated anthocyan biosynthesis, and seeds thereof.

Abstract translation: 本发明涉及以下：调节植物体花色素生物合成的方法，其包括在植物中控制编码拟南芥衍生的AtPTR2蛋白的基因表达的步骤; 具有调节花色素生物合成的转化植物体的生产方法包括通过将包含编码AtPTR2蛋白的基因的重组载体转化到植物体上来控制编码AtPTR2蛋白的基因的表达的步骤; 以及用于调节植物体的花青生物合成的组合物，包括编码AtPTR2蛋白的基因，具有调节的花青生物合成的转化植物体及其种子。