公开(公告)号：KR1020140118079A

公开(公告)日：2014-10-08

申请号：KR1020130033345

申请日：2013-03-28

Applicant: 한국해양과학기술원

Inventor： 강성균 ,  이정현 ,  권개경 ,  이현숙 ,  김태완 ,  김윤재 ,  김민식 ,  전정호

IPC: C12N1/21 ,  C12N15/63 ,  C12P3/00

CPC classification number: C12P3/00 ,  C12N9/0067 ,  C12Y112/98001

Abstract: The present invention relates to a Thermococcus onnurineus NA1 mutant having improved hydrogen producibility from formate and a method for producing hydrogen using the same. According to the present, the mutated Thermococcus onnurineus NA1 has improved hydrogen producibility in a medium including formate rather than a wild type, and improved growth velocity compared to the wild type. If a strain according to the present invention is used, hydrogen can be produced from formic acid at high efficiency.

Abstract translation: 本发明涉及具有从甲酸盐提高的氢生产能力的嗜热热球菌NA1突变体和使用其产生氢的方法。 根据本发明，突变的Thermococcus onnurineus NA1在包括甲酸而不是野生型的培养基中具有改善的氢生产能力，并且与野生型相比改善了生长速度。 如果使用根据本发明的菌株，则可以高效地从甲酸生产氢。

公开(公告)号：KR1020140025686A

公开(公告)日：2014-03-05

申请号：KR1020120091634

申请日：2012-08-22

Applicant: 한국해양과학기술원

Inventor： 이정현 ,  권개경 ,  강성균 ,  이현숙 ,  차선신 ,  전정호 ,  김윤재 ,  양영렬 ,  강민선

IPC: C12N9/78 ,  C12N15/57 ,  C12P17/10

CPC classification number: C12N9/80 ,  C12P17/10 ,  C12Y305/01004

Abstract: The present invention relates to novel amidase, a gene encoding thereof, and a production method of caprolactam by using the amidase and the gene. The amidase of the present invention is capable of producing alpha-amino-epsilon-caprolactam from lysine. The alpha-amino-epsilon-caprolactam is a material for nylon and is internationally mass-produced. Therefore, the present invention provides a novel method of producing the alpha-amino-epsilon-caprolactam which is an environmentally friendly material for nylon.

Abstract translation: 本发明涉及新型酰胺酶，其编码基因，以及使用酰胺酶和基因的己内酰胺的制造方法。 本发明的酰胺酶能够从赖氨酸产生α-氨基 - 己内酰胺。 α-氨基-ε-己内酰胺是用于尼龙的材料，并且是国际上批量生产的。 因此，本发明提供了一种生产用于尼龙的环保材料的α-氨基 - ε-己内酰胺的新方法。

公开(公告)号：KR1020140022082A

公开(公告)日：2014-02-21

申请号：KR1020137034430

申请日：2009-09-07

Applicant: 한국해양과학기술원

Inventor： 이정현 ,  강성균 ,  이현숙 ,  김상진 ,  권개경 ,  차선신 ,  전정호 ,  임형순 ,  김윤재 ,  배승섭 ,  임재규 ,  김민식 ,  김태완 ,  류지영 ,  정재연

IPC: C12N9/02 ,  C12N15/53 ,  C12P3/00

CPC classification number: C12N9/0006 ,  C12N9/0067 ,  C12P3/00 ,  C12R1/01 ,  Y02P20/132 ,  Y02P20/52

Abstract: The present invention relates to novel hydrogenases separated from new hyperthermophilic strains belonging to Thermococcus genus, genes encoding the same, and a method for producing hydrogen using the same. According to a method for producing hydrogen of the present invention, a large amount of hydrogen can be produced by culturing the strains in a specific culture condition and therefore, it is more economical and effective compared to the existing method for producing hydrogen and hydrogen can be produced at a high temperature.

Abstract translation: 本发明涉及从属于Thermococcus属的新的超嗜热菌株分离出的新型氢化酶，以及使用该氢过氧化氢的方法。 根据本发明的氢的制造方法，可以通过在特定培养条件下培养菌株来生产大量氢，因此与现有的氢气制备方法相比，其更经济有效，氢可以是 在高温下生产。

公开(公告)号：KR1020140022081A

公开(公告)日：2014-02-21

申请号：KR1020137034429

申请日：2009-09-07

Applicant: 한국해양과학기술원

Inventor： 이정현 ,  강성균 ,  이현숙 ,  김상진 ,  권개경 ,  차선신 ,  전정호 ,  임형순 ,  김윤재 ,  배승섭 ,  임재규 ,  김민식 ,  김태완 ,  류지영 ,  정재연

IPC: C12N9/02 ,  C12N15/53 ,  C12P3/00

CPC classification number: C12N9/0006 ,  C12N9/0067 ,  C12P3/00 ,  C12R1/01 ,  Y02P20/132 ,  Y02P20/52

Abstract: The present invention relates to novel hydrogenases separated from new hyperthermophilic strains belonging to Thermococcus genus, genes encoding the same, and a method for producing hydrogen using the same. According to a method for producing hydrogen of the present invention, a large amount of hydrogen can be produced by culturing the strains in a specific culture condition and therefore, it is more economical and effective compared to the existing method for producing hydrogen and hydrogen can be produced at a high temperature.

Abstract translation: 本发明涉及从属于Thermococcus属的新的超嗜热菌株分离出的新型氢化酶，以及使用该氢过氧化氢的方法。 根据本发明的氢的制造方法，可以通过在特定培养条件下培养菌株来生产大量氢，因此与现有的氢气制备方法相比，其更经济有效，氢可以是 在高温下生产。

公开(公告)号：KR1020130115496A

公开(公告)日：2013-10-22

申请号：KR1020120037823

申请日：2012-04-12

Applicant: 한국해양과학기술원

Inventor： 이정현 ,  강성균 ,  이현숙 ,  권개경 ,  임형순 ,  김윤재 ,  배승섭 ,  김태완

IPC: C12M1/107 ,  C12P3/00 ,  C01B3/02 ,  C12N1/20

CPC classification number: C12M21/04 ,  C01B3/02 ,  C12M23/36 ,  C12M23/42 ,  C12M23/58 ,  C12M29/26

Abstract: PURPOSE: A carbon monoxide dissolution device for producing hydrogen using anaerobic microorganisms is provided to help hydrogen-producing anaerobic microorganisms to effectively use carbon monoxides when hydrogen is produced, thereby enhancing hydrogen production efficiency. CONSTITUTION: A carbon monoxide dissolution device (100) for producing hydrogen using anaerobic microorganisms comprises: a main body (110) with an opening (111); tube cassettes (120) which have a tube for allowing a culture medium containing hydrogen-producing anaerobic microorganisms and carbon monoxides to pass; and a fitting member (130) which provides a delayed path for delaying time for dissolving the carbon monoxides. The tubes have supply ports (123) and outlets (124) by connecting the tubes between the tube cassettes. The both ends of the tubes are exposed to the outside to help the tube to be connected to other tubes. [Reference numerals] (230) Carbon monoxide supplying unit; (240) Hydrogen collecting unit; (250) Cooling unit; (260) Culture medium supplying unit

Abstract translation: 目的：提供一种使用厌氧微生物生产氢气的一氧化碳溶解装置，用于在生产氢气时帮助产氢厌氧微生物有效地使用一氧化碳，从而提高氢生产效率。 构成：使用厌氧微生物生产氢的一氧化碳溶解装置（100）包括：具有开口（111）的主体（110）; 管筒（120）具有用于允许含有产氢厌氧微生物和一氧化碳的培养基通过的管; 以及装配构件（130），其提供用于延迟用于溶解一氧化碳的时间的延迟路径。 管通过将管连接在管盒之间而具有供给口（123）和出口（124）。 管的两端暴露于外部，以帮助管连接到其他管。 （附图标记）（230）一氧化碳供给单元; （240）集氢单元; （250）冷却单元; （260）培养基供应单元

公开(公告)号：KR1020130071976A

公开(公告)日：2013-07-01

申请号：KR1020110139494

申请日：2011-12-21

Applicant: 한국해양과학기술원

Inventor： 서현석 ,  권개경 ,  정경태

IPC: B09C1/02 ,  B01D17/02 ,  C11D3/382 ,  C02F1/40

CPC classification number: B09C1/02 ,  B01D17/02 ,  C02F1/40 ,  C11D3/382

Abstract: PURPOSE: P-organic acid mixing solvent and a removing method of weathered oil using the same are provided to remove the weathered oil drifted ashore on the seashore bedrock easily and environment-friendlily. CONSTITUTION: A P-organic acid mixing bio solvent comprises 80% of P-organic acid and 20% of soybean oil. The removing method of weathered oil drifted ashore on the seashore bedrock comprises: a step of setting up an oil fence in the corresponding area; a step of spraying the P-organic mixing bio solvent on the drifted oil at low tide and re-spraying after considerable space of time; a step of inducing floating of separated oil and P-organic acid mixing bio solvent fallen out from bedrocks, etc. by flood tide; and a step of removing the floating oil and P-organic acid mixing bio solvent using an oil absorption paper.

Abstract translation: 目的：提供P-有机酸混合溶剂和使用该有机酸混合溶剂的风化油除去方法，轻松，环境友好地除去海滨基岩上岸的风化油。 构成：P-有机酸混合生物溶剂包括80％的P - 有机酸和20％的大豆油。 沿海基岩漂浮的风化油除去方法包括：在相应区域设置油栅栏的步骤; 在低潮时喷洒P有机混合生物溶剂在漂浮的油上并在相当长的时间后再喷涂的步骤; 诱发浮选分离油和P-有机酸混合生物溶剂的步骤由于潮汐从基岩等下降; 以及使用吸油纸除去浮油和P-有机酸混合生物溶剂的步骤。

公开(公告)号：KR1020110126012A

公开(公告)日：2011-11-22

申请号：KR1020100045692

申请日：2010-05-14

Applicant: 한국해양과학기술원

Inventor： 이정현 ,  강성균 ,  김상진 ,  권개경 ,  이현숙 ,  김윤재 ,  배승섭 ,  임재규 ,  전정호 ,  조요나 ,  황영옥 ,  차선신

IPC: C12N9/12 ,  C12N15/64 ,  C12N15/66 ,  C12N15/70

CPC classification number: C12N9/12 ,  C12N15/64 ,  C12N15/66 ,  C12N15/70

Abstract: PURPOSE: A DNA polymerase F7 and genes thereof are provided to enhance elongation and processivity and to be used in PCR. CONSTITUTION: A DNA polymerase F7 contains an amino acid sequence of sequence number 2. A DNA polymerase F7 gene encoding an amino acid sequence of sequence number 2 contains a base of sequence number 1. A recombinant vector contains the polymerase F7 gene of sequence number 1. A host cell is transformed with the recombinant vector containing the DNA polymerase F7 gene.

Abstract translation: 目的：提供DNA聚合酶F7及其基因以增加伸长和持续性，并用于PCR。 构成：DNA聚合酶F7含有序列号2的氨基酸序列。编码序列号2的氨基酸序列的DNA聚合酶F7基因含有序列号1的碱基。重组载体含有序列号1的聚合酶F7基因 用含有DNA聚合酶F7基因的重组载体转化宿主细胞。

公开(公告)号：KR1020110094092A

公开(公告)日：2011-08-19

申请号：KR1020117014737

申请日：2009-09-07

Applicant: 한국해양과학기술원

Inventor： 이정현 ,  강성균 ,  이현숙 ,  김상진 ,  권개경 ,  차선신 ,  전정호 ,  임형순 ,  김윤재 ,  배승섭 ,  임재규 ,  김민식 ,  김태완 ,  류지영 ,  정재연

IPC: C12N9/02 ,  C12N15/53 ,  C12P3/00 ,  C12N15/31

CPC classification number: C12N9/0006 ,  C12N9/0067 ,  C12P3/00 ,  C12R1/01 ,  Y02P20/132 ,  Y02P20/52

Abstract: PURPOSE: A novel hydrogenase isolated from Thermococcus spp. and a method for producing hydrogen using the same are provided to produce hydrogen without high temperature and pressure. CONSTITUTION: A hydrogenase contains an amino acid sequence of sequence number 5. A gene encoding the hydrogenase contains a base sequence of sequence number 16. A method for producing hydrogen from Thermococcus spp. comprises: a step of preparing a medium; a step of culturing Thermococcus spp. in the medium; and a step of isolating hydrogen. The medium contains carbon monoxide. The Thermococcus spp. is Thermococcus onnurineus NA1(deposit number: KCTC 10859BP).

Abstract translation: 目的：从Thermococcus spp。分离出的新型氢化酶。 并且提供使用其制造氢的方法以在没有高温和高压的情况下产生氢。 构成：氢化酶含有序列号5的氨基酸序列。编码氢化酶的基因含有序列号16的碱基序列。从Thermococcus spp。生产氢的方法。 包括：制备介质的步骤; 培养热球菌属的一个步骤。 在媒体中 和分离氢的步骤。 介质含有一氧化碳。 热球菌属 是Thermococcus onnurineus NA1（保藏号：KCTC 10859BP）。

公开(公告)号：KR1020110093912A

公开(公告)日：2011-08-18

申请号：KR1020117014736

申请日：2009-09-07

Applicant: 한국해양과학기술원

Inventor： 이정현 ,  강성균 ,  이현숙 ,  김상진 ,  권개경 ,  차선신 ,  전정호 ,  임형순 ,  김윤재 ,  배승섭 ,  임재규 ,  김민식 ,  김태완 ,  류지영 ,  정재연

IPC: C12N9/02 ,  C12N15/53 ,  C12P3/00 ,  C12N1/21

CPC classification number: C12N9/0006 ,  C12N9/0067 ,  C12P3/00 ,  C12R1/01 ,  Y02P20/132 ,  Y02P20/52

Abstract: PURPOSE: A method for producing hydrogen using hydrogenase isolated from Thermococcus spp. is provided to cheaply and efficiently produce hydrogen even at high temperature. CONSTITUTION: A hydrogenase contains an amino acid sequence of sequence number 7. A gene encoding the hydrogenase contains a base sequence of sequence number 18. A method for producing hydrogen from Thermococcus spp. comprises: a step of preparing a medium; a step of culturing Thermococcus spp. in the medium at 80°C under the anaerobic condition; and a step of isolating hydrogen. The Thermococcus spp. is Thermococcus onnurineus NA1(deposit number: KCTC 10859BP). The medium contains formate.

Abstract translation: 目的：使用从Thermococcus spp分离的氢化酶产生氢的方法。 即使在高温下也能廉价高效地生产氢。 构成：氢化酶含有序列号7的氨基酸序列。编码氢化酶的基因含有序列号18的碱基序列。一种从Thermococcus spp。生产氢的方法。 包括：制备介质的步骤; 培养热球菌属的一个步骤。 在80℃的培养基中，在厌氧条件下; 和分离氢的步骤。 热球菌属 是Thermococcus onnurineus NA1（保藏号：KCTC 10859BP）。 培养基含有甲酸。

公开(公告)号：KR1020110069744A

公开(公告)日：2011-06-23

申请号：KR1020107013071

申请日：2009-09-07

Applicant: 한국해양과학기술원

Inventor： 이정현 ,  강성균 ,  이현숙 ,  김상진 ,  권개경 ,  차선신 ,  전정호 ,  조요나 ,  김윤재 ,  배승섭 ,  임재규 ,  정인순

IPC: C12N9/02 ,  C12P3/00 ,  C12R1/01 ,  C12N9/04

CPC classification number: C12N9/0006 ,  C12N9/0067 ,  C12P3/00 ,  C12R1/01 ,  Y02P20/132 ,  Y02P20/52 ,  C12Y112/01004

Abstract: PURPOSE: A novel hydrogenase isolated from Thermococcus sp. and a method for producing hydrogen using the same are provided to produce a large amount of hydrogen even at high temperature. CONSTITUTION: A hydrogenase has an amino acid sequence selected from sequence numbers 1-8. A gene encoding the hydrogenase contains a base sequence selected from sequence numbers 12-19. A method for producing hydrogen from Thermococcus spp. comprises: a step of preparing a medium; a step of culturing Thermococcus spp. in a culture container; and a step of isolating hydrogen from culture container. The Thermococcus spp. is Thermococcus onnurineus NA1(deposit number: KCTC 10859BP). The medium contains carbon monoxide, alkyl formate, or starch. The culture is performed at 80°C and under anaerobic condition.

Abstract translation: 目的：从Thermococcus sp。 提供使用其制造氢的方法，即使在高温下也能产生大量的氢。 构成：氢化酶具有选自序列号1-8的氨基酸序列。 编码氢化酶的基因含有选自序列号12-19的碱基序列。 一种从Thermococcus spp。生产氢的方法 包括：制备介质的步骤; 培养热球菌属的一个步骤。 在文化容器中 以及从培养容器中分离氢的步骤。 热球菌属 是Thermococcus onnurineus NA1（保藏号：KCTC 10859BP）。 培养基含有一氧化碳，甲酸烷基酯或淀粉。 培养在80℃和无氧条件下进行。