신규한 에폭시드 가수분해효소 및 이의 용도
    4.
    发明公开
    신규한 에폭시드 가수분해효소 및 이의 용도 无效
    一种新型环氧化物水解物及其用途

    公开(公告)号:KR1020110061837A

    公开(公告)日:2011-06-10

    申请号:KR1020090118351

    申请日:2009-12-02

    CPC classification number: C12N9/14 C12N15/67 C12Y303/02009

    Abstract: PURPOSE: A novel epoxide hydrolase is provided to hydrolyze non-degradable epoxide and to attenuate SO cytotoxicity. CONSTITUTION: An expoxide hydrolase has an amino acid sequence of sequence number 1. A method for preparing the epoxide hydrolase comprises: a step of inserting a gene encoding the epoxide hydrolase to an expression vector to prepare a recombinant vector; a step of introducing the recombinant vector to a host cell for overexpression; and a step of isolating and purifying the overexpressed apoxide hydrolase. The epoxy substrate is non-degradable epoxide substrate, styrene 7,8-oxide.

    Abstract translation: 目的:提供一种新的环氧化物水解酶来水解不可降解的环氧化物并减弱SO细胞毒性。 一种环氧化物水解酶具有序列号1的氨基酸序列。制备环氧化物水解酶的方法包括:将编码环氧化物水解酶的基因插入表达载体以制备重组载体的步骤; 将重组载体导入宿主细胞以进行过表达的步骤; 以及分离和纯化过表达的过氧化物水解酶的步骤。 环氧基材是不可降解的环氧化物底物,苯乙烯7,8-氧化物。

    롱 PCR이 가능한 DNA 중합효소 및 이의 유전자들
    6.
    发明公开
    롱 PCR이 가능한 DNA 중합효소 및 이의 유전자들 有权
    用于长PCR的DNA聚合酶及其基因

    公开(公告)号:KR1020130030799A

    公开(公告)日:2013-03-27

    申请号:KR1020137000005

    申请日:2010-05-14

    CPC classification number: C12N9/1252

    Abstract: PURPOSE: A DNA polymerase is provided to ensure superior performance in PCR of long chain DNA and to be applied in various fields. CONSTITUTION: A DNA polymerase has an amino acid sequence of sequence number 2. A DNA polymerase gene encodes an amino acid sequence of sequence number 2. The DNA polymerase gene is sequence number 1. A recombinant vector contains the DNA polymerase gene. A host cell is transformed by the recombinant vector. A method for preparing the polymerase comprises a step of inducing expression of the recombinant protein and isolating the polymerase.

    Abstract translation: 目的:提供DNA聚合酶,以确保长链DNA PCR的优异性能并应用于各种领域。 构成:DNA聚合酶具有序列号2的氨基酸序列.DNA聚合酶基因编码序列号2的氨基酸序列.DNA聚合酶基因是序列号1.重组载体含有DNA聚合酶基因。 宿主细胞被重组载体转化。 制备聚合酶的方法包括诱导重组蛋白表达并分离聚合酶的步骤。

    DNA 중합효소 P7 및 이의 유전자들
    7.
    发明公开
    DNA 중합효소 P7 및 이의 유전자들 无效
    DNA聚合酶P7及其基因

    公开(公告)号:KR1020110126009A

    公开(公告)日:2011-11-22

    申请号:KR1020100045689

    申请日:2010-05-14

    Abstract: PURPOSE: A DNA polymerase P7 and genes thereof are provided to ensure excellent processivity, fidelity, and elongation length performance. CONSTITUTION: A DNA polymerase P7 contains an amino acid sequence of sequence number 2. A DNA polymerase P7 gene encodes an amino acid sequence of sequence number 2. The DNA polymerase P7 gene contains a base sequence of sequence number 1. A recombinant vector contains the P7 gene. A host cell is transformed with the recombinant vector containing the P7 gene. The DNA polymerase P7 is derived from Thermococcus sp.

    Abstract translation: 目的:提供DNA聚合酶P7及其基因以确保优异的持续性,保真度和伸长长度性能。 构成:DNA聚合酶P7含有序列号2的氨基酸序列.DNA聚合酶P7基因编码序列号2的氨基酸序列.DNA聚合酶P7基因含有序列号1的碱基序列。重组载体含有 P7基因。 用含有P7基因的重组载体转化宿主细胞。 DNA聚合酶P7衍生自Thermococcus sp。

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