써모코커스 바로씨로부터 분리된 신규의 베타-자일로시다제 및 이의 제조방법
    5.
    发明公开
    써모코커스 바로씨로부터 분리된 신규의 베타-자일로시다제 및 이의 제조방법 审中-实审
    来自热酵母BAROSSII的新型β-谷胱甘肽和其制备方法

    公开(公告)号:KR1020140144443A

    公开(公告)日:2014-12-19

    申请号:KR1020130066378

    申请日:2013-06-11

    Abstract: 본 발명은 써모코커스 바로씨(
    Thermococcus barossii )로부터 분리된 신규의 베타-자일로시다제 및 이의 제조방법에 관한 것으로서, 서열 번호 2의 아미노산 서열을 가진 베타-자일로시다제 및 이를 암호화하는 핵산서열과 상기 핵산서열을 포함하는 재조합벡터, 상기 재조합벡터로 형질전환된 숙주 및 상기 숙주 또는 써모코커스 바로씨를 이용한 베타-자일로시다제 생산 방법에 관한 것이다.

    Abstract translation: 本发明涉及从耐热球菌(Thermococcus barossii)分离的新型β-木糖苷酶及其制备方法。 更具体地,本发明涉及:具有SEQ ID:2的氨基酸序列的β-木糖苷酶; 编码该氨基酸序列的氨基酸序列; 包含相同氨基酸序列的重组载体; 由相同重组载体转化的宿主; 以及使用相同宿主或Thermococcus barosii制备β-木糖苷酶的方法。

    심해 해저에서 분리된 에스터라제 KTL 9
    8.
    发明公开
    심해 해저에서 분리된 에스터라제 KTL 9 有权
    从深海沉积物中分离的酯酶KTL 9

    公开(公告)号:KR1020120129852A

    公开(公告)日:2012-11-28

    申请号:KR1020120116123

    申请日:2012-10-18

    Abstract: PURPOSE: An esterase KTL9 which is separated from the bottom of the sea is provided to enhance activities and to have stability in wide range of temperature. CONSTITUTION: An esterase KTL9 which is separated from the bottom of the sea has amino acid sequence of the sequence number 34. A gene ciphering the esterase has the base sequence described in the sequence number 33. A manufacturing method of the esterase gene comprises the following steps: transforming the cells into recombinant vector which includes the esterase; culturing the transformed cells; and separating the esterase from the cultured cell.

    Abstract translation: 目的:提供与海底分离的酯酶KTL9,以增强活性并在宽温度范围内具有稳定性。 构成:从海底分离的酯酶KTL9具有序列号34的氨基酸序列。加密酯酶的基因具有序列号33中所述的碱基序列。酯酶基因的制造方法包括以下 步骤:将细胞转化成包含酯酶的重组载体; 培养转化细胞; 并从培养的细胞中分离酯酶。

    DNA 중합효소 T7 및 이의 유전자들
    9.
    发明公开
    DNA 중합효소 T7 및 이의 유전자들 无效
    DNA聚合酶T7及其基因

    公开(公告)号:KR1020110126011A

    公开(公告)日:2011-11-22

    申请号:KR1020100045691

    申请日:2010-05-14

    Abstract: PURPOSE: A DNA polymerase T7 and genes thereof are provided to ensure processivity, fidelity, and elongation length and to be applied in various fields. CONSTITUTION: A DNA polymerase T7 has an amino acid sequence of sequence number 2. A DNA polymerase T7 gene encodes an amino acid sequence of sequence number 2. The DNA polymerase T7 gene contains a base sequence of sequence number 1. A recombinant vector contains the T7 gene. The polymerase T7 is prepared by culturing a host cells transformed by the recombinant vector, inducing the recombinant protein expression, and isolating polymerase protein. The recombinant vector contains the DNA polymerase T7 gene. The DNA polymerase T7 is derived from Thermococcus sp. NA1.

    Abstract translation: 目的:提供DNA聚合酶T7及其基因,以确保持续性,保真度和伸长率,并适用于各种领域。 构成:DNA聚合酶T7具有序列号2的氨基酸序列.DNA聚合酶T7基因编码序列号2的氨基酸序列.DNA聚合酶T7基因含有序列号1的碱基序列。重组载体含有 T7基因。 通过培养由重组载体转化的宿主细胞,诱导重组蛋白质表达和分离聚合酶蛋白来制备聚合酶T7。 重组载体含有DNA聚合酶T7基因。 DNA聚合酶T7衍生自Thermococcus sp。 NA1。

    단백질분해효소 및 이를 코딩하는 유전자
    10.
    发明公开
    단백질분해효소 및 이를 코딩하는 유전자 无效
    编码它们的基因和基因

    公开(公告)号:KR1020090063644A

    公开(公告)日:2009-06-18

    申请号:KR1020070131091

    申请日:2007-12-14

    Abstract: A protease and gene coding the same are provided to ensure excellent algicidal activity and usefully prevent the red tide. A protease comprises amino acids of sequence number 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137 or 139. A polynucleotide coding the protease comprises base of the sequence number 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138 or 140. The protease is secreted from a kordia algicida OT-1(KCTC 8814P) strain which kills a skeletonema costatum.

    Abstract translation: 提供了一种蛋白酶及其编码基因,以确保优异的杀藻活性,有效防止红潮。 一种蛋白酶包含序列号1,3,5,7,9,11,13,15,17,19,21,23,25,27,29,31,33,35,37,39,41,46,33,39,39,39,39,39,39,31,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39 43,45,47,49,51,53,55,57,59,61,63,65,67,69,71,73,75,77,79,81,83,85,87,89, 93,95,97,99,101,103,105,107,109,111,113,115,117,119,121,123,125,127,129,131,133,135,137或139.一种多核苷酸 编码蛋白酶的步骤包括序列号2,4,6,8,10,12,14,16,18,20,22,24,26,28,30,32,34,36,38,40,42的碱基 ,44,46,48,50,52,54,56,58,60,62,64,66,68,70,72,74,76,78,80,82,84,86,88,90,92 ,94,96,98,100,102,104,106,108,110,112,114,116,118,120,122,124,126,128,130,132,134,136,138或140中。 蛋白酶是从一个kordia algicida OT-1(KCTC 8814P)菌株分泌的,该菌株杀死骨骼水肿。

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