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公开(公告)号：KR101401603B1
公开(公告)日：2014-06-27
申请号：KR1020120065303
申请日：2012-06-19
Applicant: 한국해양과학기술원
CPC classification number: C12N15/67 , C07K14/195 , C12N9/0008 , C12P3/00
Abstract: 본 발명은 수소생산능이 증가된 써모코코스 온누리우스 엔에이원 돌연변이 균주 및 이를 이용한 수소생산방법에 관한 것으로, 보다 구체적으로는 CO 디하이드로게나아제, 하이드로게나아제(
mch ) 및 복합-구조체 Na
+ /H
+ 안티포터(antiporter) (
mnh3 )유전자 클러스터의 발현율이 증가된 것을 특징으로 하는 수소생산능력이 증가된
Thermococcus
onnurineus NA1 및 이를 이용한 수소 생산방법에 관한 것이다.-
公开(公告)号：KR101383546B1
公开(公告)日：2014-04-10
申请号：KR1020070104219
申请日：2007-10-16
Applicant: 한국해양과학기술원
Abstract: 본 발명은 심해 해저에서 분리된 리파제 및 에스터라제 효소 및 이의 제조방법에 관한 것으로서, 서남태평양 해역(남위 3°89′, 동경 152°49′) 깊이 1,400 미터에 이르는 심해 퇴적물, 북극 다산기지 주변 연안 퇴적물, 강화도 갯벌 퇴적물로부터 구축된 메타게놈 라이브러리를 대상으로 분리한 신규 리파제 및 에스터라제 효소, 이들을 암호화 하는 유전자 및 리파제 및 에스터라제 효소 생산방법을 제공한다.
리파제, 에스터라제-
公开(公告)号：KR1020130142251A
公开(公告)日：2013-12-30
申请号：KR1020120065303
申请日：2012-06-19
Applicant: 한국해양과학기술원
CPC classification number: C12N15/67 , C07K14/195 , C12N9/0008 , C12P3/00
Abstract: The present invention relates to a mutated thermococcus onnurineus NA1 with increased hydrogen productivity and a method for producing hydrogen using the same, and more specifically, to a mutated thermococcus onnurineus NA1 with increased hydrogen productivity and a method for producing hydrogen using the same, wherein the expression of a CO dehydrogenase, a hydrogenase (mch), a complex-structure Na+/H+ antiporter(mnh3) gene cluster is improved.
Abstract translation: 本发明涉及具有增加的氢生产率的突变型热带炎热球菌NA1和使用其的氢的制造方法，更具体地说，涉及具有增加的氢生产率的突变的on鱼属NA1和使用其的氢的制造方法，其中 CO脱氢酶，氢化酶（mch），复合结构Na + / H +反转录酶（mnh3）基因簇的表达得到改善。
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公开(公告)号：KR1020130115495A
公开(公告)日：2013-10-22
申请号：KR1020120037822
申请日：2012-04-12
Applicant: 한국해양과학기술원
CPC classification number: C12M21/04 , C01B3/02 , C12M23/36 , C12M23/42 , C12M23/58 , C12M29/26 , C12M41/12
Abstract: PURPOSE: A hydrogen producing device using anaerobic microorganisms is provided to enhance hydrogen production efficiency to allow hydrogen-producing anaerobic microorganisms to use carbon monoxides when hydrogen is produced. CONSTITUTION: A hydrogen producing device (100) using anaerobic microorganisms comprises: a culture machine (110) which stores a culture medium containing hydrogen-producing anaerobic microorganisms; a circulation line (120) which is connected to the culture machine to discharge the culture medium from the culture machine and has a pump (121) for circulation of the culture medium; a carbon monoxide supply unit (130) which supplies carbon monoxides to the culture medium; a dissolution unit (140) with a tube which provides a delayed path for delaying time for dissolving the carbon monoxides; and a hydrogen collection unit (150) which captures hydrogen from gas separated from the culture medium. [Reference numerals] (130) Carbon monoxide supplying unit; (148) Control unit; (150) Hydrogen collecting unit; (160) Cooling unit; (170) Culture medium supplying unit
Abstract translation: 目的：提供使用厌氧微生物的氢气生产装置，以提高制氢效率，使氢气产生厌氧微生物在生产氢气时使用一氧化碳。 构成：使用厌氧微生物的制氢装置（100）包括：储存含有产氢厌氧微生物的培养基的培养机（110） 与培养机连接以从培养机排出培养基的循环管线（120），并具有用于培养培养基循环的泵（121）; 向培养基供给一氧化碳的一氧化碳供给单元（130） 溶解单元（140），其具有提供用于延迟溶解所述一氧化碳的时间的延迟路径的管; 和从所述培养基分离的气体捕获氢的氢气收集单元（150）。 （附图标记）（130）一氧化碳供给单元; （148）控制单元; （150）集氢单元; （160）冷却单元; （170）培养基供给单元
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15.发明公开
公开(公告)号：KR1020130114025A
公开(公告)日：2013-10-16
申请号：KR1020130100953
申请日：2013-08-26
Applicant: 한국해양과학기술원
Abstract: PURPOSE: A recombinant microorganism is provided to show activation and growth at high temperatures and to enable culturing at high temperatures, thereby being widely used. CONSTITUTION: A recombinant microorganism which grows at high temperatures contains lon proteins of sequence number 4. The microorganism is prokaryotes. The microorganism is gram-negative bacteria, E. coli. Virus is thermally stabilized using lon protein of sequence number 4. A thermal stabilization method of the virus comprises the steps of: isolating lon protein of sequence number 4; and making the isolated proteins and the virus come in contact. [Reference numerals] (AA) Measure survivability; (BB) Measure growth
Abstract translation: 目的：提供重组微生物以显示高温下的活化和生长，并能够在高温下培养，从而被广泛使用。 构成：在高温下生长的重组微生物含有4号序列号4的蛋白质。微生物是原核生物。 微生物是革兰氏阴性细菌，大肠杆菌。 病毒使用序列号4的蛋白质热稳定。病毒的热稳定方法包括以下步骤：分离序列号4的蛋白质; 并使分离的蛋白质和病毒接触。 （附图标记）（AA）测量生存性; （BB）测量增长
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Patent Agency Ranking
公开(公告)号：KR101736485B1
公开(公告)日：2017-05-16
申请号：KR1020130033345
申请日：2013-03-28
Applicant: 한국해양과학기술원
CPC classification number: C12P3/00 , C12N9/0067 , C12Y112/98001
Abstract: 본발명은포름산염으로부터수소생산능이증가된써모코코스온누리우스엔에이원돌연변이체및 이를이용한수소생산방법에관한것이다. 본발명에따라돌연변이화된NA1는야생형보다포름산염을포함하는배지에서수소생산능력이증가되었고, 생장속도도야생형에비해증가하였다. 본발명에따른균주를이용하면포름산으로부터고효율로수소를생산할수 있다.
Abstract translation: 本发明涉及由甲酸盐产生的具有提高的产氢能力的Thermococcus onneriensis突变体以及使用该突变体产生氢的方法。 根据本发明，与野生型相比，突变型NA1增加了含有甲酸盐的培养基中的氢生产能力和生长速率。 使用根据本发明的菌株，可以从甲酸高效生产氢。
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公开(公告)号：KR101580488B1
公开(公告)日：2015-12-28
申请号：KR1020130137344
申请日：2013-11-13
Applicant: 한국해양과학기술원
CPC classification number: C12P3/00 , C07K14/195 , C12N1/20 , C12N15/67
Abstract: 본발명은 rchA 유전자의발현이증가된것을특징으로하는수소생산능력이증가된써모코커스온누리누스 MC02(MC02, 수탁번호: KCTC12511BP) 균주를제공한다. 또한, 써모코커스속 균주의 rchA 유전자의발현을증가시키는단계를포함하는것을특징으로하는수소생산능력이증가된써모코커스속 균주제조방법을제공하고, 써모코커스온누리누스 MC02 균주를이용한수소생산방법을제공한다.
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公开(公告)号：KR1020140118079A
公开(公告)日：2014-10-08
申请号：KR1020130033345
申请日：2013-03-28
Applicant: 한국해양과학기술원
CPC classification number: C12P3/00 , C12N9/0067 , C12Y112/98001
Abstract: The present invention relates to a Thermococcus onnurineus NA1 mutant having improved hydrogen producibility from formate and a method for producing hydrogen using the same. According to the present, the mutated Thermococcus onnurineus NA1 has improved hydrogen producibility in a medium including formate rather than a wild type, and improved growth velocity compared to the wild type. If a strain according to the present invention is used, hydrogen can be produced from formic acid at high efficiency.
Abstract translation: 本发明涉及具有从甲酸盐提高的氢生产能力的嗜热热球菌NA1突变体和使用其产生氢的方法。 根据本发明，突变的Thermococcus onnurineus NA1在包括甲酸而不是野生型的培养基中具有改善的氢生产能力，并且与野生型相比改善了生长速度。 如果使用根据本发明的菌株，则可以高效地从甲酸生产氢。
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公开(公告)号：KR1020140025686A
公开(公告)日：2014-03-05
申请号：KR1020120091634
申请日：2012-08-22
Applicant: 한국해양과학기술원
CPC classification number: C12N9/80 , C12P17/10 , C12Y305/01004
Abstract: The present invention relates to novel amidase, a gene encoding thereof, and a production method of caprolactam by using the amidase and the gene. The amidase of the present invention is capable of producing alpha-amino-epsilon-caprolactam from lysine. The alpha-amino-epsilon-caprolactam is a material for nylon and is internationally mass-produced. Therefore, the present invention provides a novel method of producing the alpha-amino-epsilon-caprolactam which is an environmentally friendly material for nylon.
Abstract translation: 本发明涉及新型酰胺酶，其编码基因，以及使用酰胺酶和基因的己内酰胺的制造方法。 本发明的酰胺酶能够从赖氨酸产生α-氨基 - 己内酰胺。 α-氨基-ε-己内酰胺是用于尼龙的材料，并且是国际上批量生产的。 因此，本发明提供了一种生产用于尼龙的环保材料的α-氨基 - ε-己内酰胺的新方法。
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20.发明公开개미산을 이용해 수소를 생산해 낼 수 있는 Thermococcus spp. 로부터 분리된 신규한 서열번호 7의 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법 有权序列号7的新型氢化物，从热能SPP中纯化。 通过使用FORMATE，编码它们的基因，以及使用具有基因的微生物生产氢的方法
公开(公告)号：KR1020140022082A
公开(公告)日：2014-02-21
申请号：KR1020137034430
申请日：2009-09-07
Applicant: 한국해양과학기술원
CPC classification number: C12N9/0006 , C12N9/0067 , C12P3/00 , C12R1/01 , Y02P20/132 , Y02P20/52
Abstract: The present invention relates to novel hydrogenases separated from new hyperthermophilic strains belonging to Thermococcus genus, genes encoding the same, and a method for producing hydrogen using the same. According to a method for producing hydrogen of the present invention, a large amount of hydrogen can be produced by culturing the strains in a specific culture condition and therefore, it is more economical and effective compared to the existing method for producing hydrogen and hydrogen can be produced at a high temperature.
Abstract translation: 本发明涉及从属于Thermococcus属的新的超嗜热菌株分离出的新型氢化酶，以及使用该氢过氧化氢的方法。 根据本发明的氢的制造方法，可以通过在特定培养条件下培养菌株来生产大量氢，因此与现有的氢气制备方法相比，其更经济有效，氢可以是 在高温下生产。
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