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公开(公告)号：KR1020120129851A
公开(公告)日：2012-11-28
申请号：KR1020120116121
申请日：2012-10-18
Applicant: 한국해양과학기술원
Abstract: PURPOSE: An esterase KTL7 which is separated from the bottom of the sea is provided to enhance activities and to have stability in wide range of temperature. CONSTITUTION: An esterase KTL7 which is separated from the bottom of the sea has amino acid sequence of the sequence number 32(SEQ ID NO:32). A gene ciphering the esterase has the base sequence described in the sequence number 31. A manufacturing method of the esterase gene comprises the following steps: transforming the cells into recombinant vector which includes the esterase of the sequence number 31; culturing the transformed cells; and separating the esterase from the cultured cell. The novel esterase gene is obtained by the following steps: extracting environmental DNA from the deposit of the depth of 1,400 M; building a library by using fosmid vector; and separating the gene from a clone which is active in the tricaprulin flat medium.
Abstract translation: 目的：提供与海底分离的酯酶KTL7，以增强活性，并在宽温度范围内具有稳定性。 构成：从海底分离出的酯酶KTL7具有序列号32（SEQ ID NO：32）的氨基酸序列。 加密酯酶的基因具有序列号31中描述的碱基序列。酯酶基因的制备方法包括以下步骤：将细胞转化成包含序列号31的酯酶的重组载体; 培养转化细胞; 并从培养的细胞中分离酯酶。 通过以下步骤获得新型酯酶基因：从1400m深度的沉积物中提取环境DNA; 使用fosmid载体构建库; 并将该基因与三权纲平台培养基中活性的克隆分离。
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公开(公告)号：KR1020110126009A
公开(公告)日：2011-11-22
申请号：KR1020100045689
申请日：2010-05-14
Applicant: 한국해양과학기술원
Abstract: PURPOSE: A DNA polymerase P7 and genes thereof are provided to ensure excellent processivity, fidelity, and elongation length performance. CONSTITUTION: A DNA polymerase P7 contains an amino acid sequence of sequence number 2. A DNA polymerase P7 gene encodes an amino acid sequence of sequence number 2. The DNA polymerase P7 gene contains a base sequence of sequence number 1. A recombinant vector contains the P7 gene. A host cell is transformed with the recombinant vector containing the P7 gene. The DNA polymerase P7 is derived from Thermococcus sp.
Abstract translation: 目的：提供DNA聚合酶P7及其基因以确保优异的持续性，保真度和伸长长度性能。 构成：DNA聚合酶P7含有序列号2的氨基酸序列.DNA聚合酶P7基因编码序列号2的氨基酸序列.DNA聚合酶P7基因含有序列号1的碱基序列。重组载体含有 P7基因。 用含有P7基因的重组载体转化宿主细胞。 DNA聚合酶P7衍生自Thermococcus sp。
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13.发明公开개미산을 이용해 수소를 생산해 낼 수 있는 Ｔｈｅｒｍｏｃｃｕｓ ｓｐｐ．로부터 분리된 신규한 서열번호 2의 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법 无效序列号2的新型氢化物，其从热能SPP中纯化。 通过使用FORMATE，编码它们的基因，以及使用具有基因的微生物生产氢的方法
公开(公告)号：KR1020110093913A
公开(公告)日：2011-08-18
申请号：KR1020117014743
申请日：2009-09-07
Applicant: 한국해양과학기술원
CPC classification number: C12N9/0006 , C12N9/0067 , C12P3/00 , C12R1/01 , Y02P20/132 , Y02P20/52
Abstract: PURPOSE: A hydrogenase isolated from Thermococcus spp. is provided to generate hydrogen at room temperature and pressure without harmful by-products. CONSTITUTION: A hydrogenase contains an amino acid sequence of sequence number 2. A gene encoding the hydrogenase contains a base sequence of sequence number 13. A method for producing hydrogen from Thermococcus spp. comprises: a step of culturing Thermococcus spp. in a medium containing formate at 80°C under the anaerobic condition; and a step of isolating hydrogen from a culture container.
Abstract translation: 目的：从Thermococcus spp。分离的氢化酶。 被提供以在室温和压力下产生氢气而没有有害的副产物。 构成：氢化酶含有序列号2的氨基酸序列。编码氢化酶的基因含有序列号13的碱基序列。从Thermococcus spp。生产氢的方法。 包括：培养Thermococcus spp的步骤。 在含有甲酸盐的培养基中，在80℃，无氧条件下， 以及从培养容器中分离氢的步骤。
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14.发明授权일산화탄소를 이용해 수소를 생산해 낼 수 있는 Thermococcus spp. 로부터 분리된 신규한 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법 有权Thermococcus spp。可用一氧化碳生产氢气。 使用新型氢化酶，编码其的基因和具有该基因的微生物生产氢的方法
公开(公告)号：KR101736484B1
公开(公告)日：2017-05-30
申请号：KR1020137034431
申请日：2009-09-07
Applicant: 한국해양과학기술원
CPC classification number: C12N9/0006 , C12N9/0067 , C12P3/00 , C12R1/01 , Y02P20/132 , Y02P20/52
Abstract: 본발명은속에속하는고호열성신균주로부터분리한신규한수소화효소(hydrogenase), 이를암호화하는유전자및 이들을이용하여수소를생산하는방법에관한것이다. 본발명의수소생산방법에따르면상기균주를특정배양조건으로배양하는것만으로많은양의수소를생산할수 있으므로, 기존의수소생산방법에비하여경제적이고효율적이며, 고온에서도수소를생산할수 있는장점이있다.
Abstract translation: 本发明涉及一种新的氢化酶，编码该氢化酶的基因，以及使用该氢化酶生产氢的方法。 由于根据本发明的制氢方法的应变可产生具有只培养haneungeot到特定的培养条件，以及经济和比现有的制氢方法高效，存在能够即使在高温下产生氢的优点大量氢的。
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公开(公告)号：KR1020160108384A
公开(公告)日：2016-09-19
申请号：KR1020167020699
申请日：2009-09-07
Applicant: 한국해양과학기술원
CPC classification number: C12N9/0006 , C12N9/0067 , C12P3/00 , C12R1/01 , Y02P20/132 , Y02P20/52 , C12Y112/01004
Abstract: 수소화효소(hydrogenase), 이를암호화하는유전자및 이들을이용하여수소를생산하는방법에관한것이다. 본발명의수소생산방법에따르면상기균주를특정배양조건으로배양하는것만으로많은양의수소를생산할수 있으므로, 기존의수소생산방법에비하여경제적이고효율적이며, 고온에서도수소를생산할수 있는장점이있다.
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Patent Agency Ranking
公开(公告)号：KR1020150125631A
公开(公告)日：2015-11-09
申请号：KR1020150137914
申请日：2015-09-30
Applicant: 한국해양과학기술원
Abstract: 본발명은포름산염으로부터수소생산능이증가된써모코코스온누리우스엔에이원돌연변이체및 이를이용한수소생산방법에관한것이다. 본발명에따라돌연변이화된NA1는야생형보다포름산염을포함하는배지에서수소생산능력이증가되었고, 생장속도도야생형에비해증가하였다. 본발명에따른균주를이용하면포름산으로부터고효율로수소를생산할수 있다.
Abstract translation: 本发明涉及具有提高从甲酸生成氢的能力的热球藻NA1突变体，以及使用其的氢制造方法。 通过本发明突变的Thermococcus onnurineus NA1显示出与含有甲酸的培养基相比产生氢的能力更高，与野生型相比增加生长速度，从而可以从甲酸高效地生产氢，如果使用 应变根据本发明。
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公开(公告)号：KR1020140145221A
公开(公告)日：2014-12-23
申请号：KR1020130065884
申请日：2013-06-10
Applicant: 한국해양과학기술원
Inventor： 임형순 , 강성균 , 정재연 , 차선신 , 오현명 , 이재학 , 양은찬 , 권개경 , 김윤재 , 최동한 , 김태완 , 전정호 , 김상진 , 이정현 , 이현숙 , 조윤성 , 고준수 , 박종화
Abstract: 본 발명은 밍크 고래로부터 분리된 신규의 리파제 및 이의 제조방법에 관한 것으로서, 밍크 고래 유래의 서열 번호 2, 4, 6, 8, 10, 12, 14 또는 16의 아미노산 서열을 가진 그룹에서 선택된 어느 하나의 아미노산 서열을 가진 리파제 및 이를 암호화하는 유전자와 상기 유전자를 포함하는 재조합벡터, 상기 재조합벡터로 형질전환된 세포 및 상기 세포를 이용한 리파제 생산방법에 관한 것이다.
Abstract translation: 本发明涉及从巴氏扁球菌基因组分离的新型脂肪酶及其制备方法，更具体地说，涉及具有任选选自SEQ ID NO：2,4,6 ，8,10,12,14和16来自Balaenoptera acutorostrata scammoni; 编码脂肪酶的基因; 包含该基因的重组载体; 用重组载体转化的细胞; 以及使用该细胞生产脂肪酶的方法。
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18.发明公开일산화탄소를 이용해 수소를 생산해 낼 수 있는 Thermococcus spp. 로부터 분리된 신규한 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법 有权从热敏SPP中纯化的新型氢化物。 通过使用碳黑单体，编码它们的基因，以及使用具有基因的微生物生产氢的方法
公开(公告)号：KR1020140022083A
公开(公告)日：2014-02-21
申请号：KR1020137034431
申请日：2009-09-07
Applicant: 한국해양과학기술원
CPC classification number: C12N9/0006 , C12N9/0067 , C12P3/00 , C12R1/01 , Y02P20/132 , Y02P20/52
Abstract: The present invention relates to novel hydrogenases separated from new hyperthermophilic strains belonging to Thermococcus genus, genes encoding the same, and a method for producing hydrogen using the same. According to a method for producing hydrogen of the present invention, a large amount of hydrogen can be produced only by culturing the strains in a specific culture condition and therefore, it is more economical and effective compared to the existing method for producing hydrogen and hydrogen can be produced at a high temperature.
Abstract translation: 本发明涉及从属于Thermococcus属的新的超嗜热菌株分离出的新型氢化酶，以及使用该氢过氧化氢的方法。 根据本发明的氢的制造方法，只要通过在特定培养条件下培养菌株即可产生大量的氢，因此与现有的制造氢气和氢气的方法相比，其更经济有效 在高温下生产。
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公开(公告)号：KR1020120129852A
公开(公告)日：2012-11-28
申请号：KR1020120116123
申请日：2012-10-18
Applicant: 한국해양과학기술원
Abstract: PURPOSE: An esterase KTL9 which is separated from the bottom of the sea is provided to enhance activities and to have stability in wide range of temperature. CONSTITUTION: An esterase KTL9 which is separated from the bottom of the sea has amino acid sequence of the sequence number 34. A gene ciphering the esterase has the base sequence described in the sequence number 33. A manufacturing method of the esterase gene comprises the following steps: transforming the cells into recombinant vector which includes the esterase; culturing the transformed cells; and separating the esterase from the cultured cell.
Abstract translation: 目的：提供与海底分离的酯酶KTL9，以增强活性并在宽温度范围内具有稳定性。 构成：从海底分离的酯酶KTL9具有序列号34的氨基酸序列。加密酯酶的基因具有序列号33中所述的碱基序列。酯酶基因的制造方法包括以下 步骤：将细胞转化成包含酯酶的重组载体; 培养转化细胞; 并从培养的细胞中分离酯酶。
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公开(公告)号：KR1020110126011A
公开(公告)日：2011-11-22
申请号：KR1020100045691
申请日：2010-05-14
Applicant: 한국해양과학기술원
Abstract: PURPOSE: A DNA polymerase T7 and genes thereof are provided to ensure processivity, fidelity, and elongation length and to be applied in various fields. CONSTITUTION: A DNA polymerase T7 has an amino acid sequence of sequence number 2. A DNA polymerase T7 gene encodes an amino acid sequence of sequence number 2. The DNA polymerase T7 gene contains a base sequence of sequence number 1. A recombinant vector contains the T7 gene. The polymerase T7 is prepared by culturing a host cells transformed by the recombinant vector, inducing the recombinant protein expression, and isolating polymerase protein. The recombinant vector contains the DNA polymerase T7 gene. The DNA polymerase T7 is derived from Thermococcus sp. NA1.
Abstract translation: 目的：提供DNA聚合酶T7及其基因，以确保持续性，保真度和伸长率，并适用于各种领域。 构成：DNA聚合酶T7具有序列号2的氨基酸序列.DNA聚合酶T7基因编码序列号2的氨基酸序列.DNA聚合酶T7基因含有序列号1的碱基序列。重组载体含有 T7基因。 通过培养由重组载体转化的宿主细胞，诱导重组蛋白质表达和分离聚合酶蛋白来制备聚合酶T7。 重组载体含有DNA聚合酶T7基因。 DNA聚合酶T7衍生自Thermococcus sp。 NA1。
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