公开(公告)号：BRPI0519165A2

公开(公告)日：2008-12-30

申请号：BRPI0519165

申请日：2005-12-21

Applicant: BASF AG

Inventor： HAFNER STEFAN ,  KLOPPROGGE CORINNA ,  HEROLD ANDREA ,  SCHROEDER HARTWIG ,  ZELDER OSKAR

IPC: C12N15/77 ,  C07K14/34

Abstract: An expression unit (A) that contains several promoters is new. A new expression unit (A) comprises several promoters and includes, in the 5' to 3' direction the sequence module (I). 5'-P 1-(A x-P x) n-A y-P y-3' (I) n = integer 0-10; A x and A y = same or different chemical bonds or nucleic acid linker sequences; P 1, P x and P y = same or different promoter sequences, containing at least one RNA-polymerase binding region, and at least P y also includes a ribosome-binding, 3'-terminal segment. Independent claims are also included for: (1) an expression cassette (EC) that contains, in the 5' to 3' direction, the sequence module (II); (2) vector that contains at least one EC; (3) a genetically modified microorganism (GMO) transfected with the vector of (2) or containing EC; and (4) method for preparing biosynthetic products (X) by culturing GMO. 5'-P 1-(A x-P x) n-A y-P y-G-3' (II) G : at least one nucleic acid coding sequence functionally linked to the 5'-upstream regulatory sequence.

公开(公告)号：NO20062685L

公开(公告)日：2006-09-14

申请号：NO20062685

申请日：2006-06-09

Applicant: BASF AG

Inventor： KROGER BURKHARD ,  ZELDER OSKAR ,  SCHRODER HARTWIG ,  HAFNER STEFAN ,  KIEFER PATRICK ,  WITTMANN CHRISTOPH ,  KLOPPROGGE CORINNA ,  HEINZLE ELMAR

IPC: C12P13/08 ,  C12N9/04

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., lactate dehydrogenase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of the expression of lactate dehydrogenase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

公开(公告)号：NO20062685A

公开(公告)日：2006-09-14

申请号：NO20062685

申请日：2006-06-09

Applicant: BASF AG

Inventor： KROGER BURKHARD ,  ZELDER OSKAR ,  SCHRODER HARTWIG ,  HAFNER STEFAN ,  KIEFER PATRICK ,  WITTMANN CHRISTOPH ,  KLOPPROGGE CORINNA ,  HEINZLE ELMAR

IPC: C12P13/08 ,  C12N9/04

CPC classification number: C12N9/0006 ,  C12P13/08

公开(公告)号：AU2003229691A8

公开(公告)日：2003-10-27

申请号：AU2003229691

申请日：2003-04-16

Applicant: BASF AG

Inventor： HAFNER STEFAN ,  KLOPPROGGE CORINNA ,  SCHRODER HARTWIG ,  ZELDER OSKAR ,  KROGER BURKHARD

IPC: A23K1/00 ,  A23K1/16 ,  C12N1/15 ,  C12N9/10 ,  C12N15/09 ,  C12P11/00 ,  C12P13/12 ,  C12R1/15 ,  C12N15/63

Abstract: The invention relates to methods for the fermentative production of sulfur-containing fine chemicals, in particular L-methionine, by using bacteria which express a nucleotide sequence coding for a methionine synthase (metH) gene.

公开(公告)号：NO20062494A

公开(公告)日：2006-09-06

申请号：NO20062494

申请日：2006-05-31

Applicant: BASF AG

Inventor： KROGER BURKHARD ,  ZELDER OSKAR ,  SCHRODER HARTWIG ,  HAFNER STEFAN ,  KIEFER PATRICK ,  WITTMANN CHRISTOPH ,  KLOPPROGGE CORINNA ,  HEINZLE ELMAR

IPC: C12P13/08 ,  C12N9/16

CPC classification number: C12P13/08 ,  C12N9/16

公开(公告)号：AU2004299729A1

公开(公告)日：2005-06-30

申请号：AU2004299729

申请日：2004-12-17

Applicant: BASF AG

Inventor： KIEFER PATRICK ,  KLOPPROGGE CORINNA ,  WITTMANN CHRISTOPH ,  HAFNER STEFAN ,  HEINZLE ELMAR ,  ZELDER OSKAR ,  KROGER BURKHARD ,  SCHRODER HARTWIG

IPC: C12P13/08 ,  C12N9/16

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., fructose-1,6-bisphosphatase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of fructose-1,6-bisphosphatase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

公开(公告)号：AU2004299728A1

公开(公告)日：2005-06-30

申请号：AU2004299728

申请日：2004-12-17

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  HAFNER STEFAN ,  HEINZLE ELMAR ,  SCHRODER HARTWIG ,  KROGER BURKHARD ,  KLOPPROGGE CORINNA ,  KIEFER PATRICK ,  WITTMANN CHRISTOPH

IPC: C12N9/04 ,  C12P13/08

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., lactate dehydrogenase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of the expression of lactate dehydrogenase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

公开(公告)号：AU2003258667A1

公开(公告)日：2004-04-30

申请号：AU2003258667

申请日：2003-08-26

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHRODER HARTWIG ,  HAFNER STEFAN ,  KROGER BURKHARD

IPC: A23K1/00 ,  A23K1/16 ,  C12N15/09 ,  C12P13/04 ,  C12P13/12 ,  C12R1/15

Abstract: Fermentative production of at least one sulfur-containing fine chemical (I) by fermenting a (I)-producing coryneform bacterium (A), concentrating (I) in medium or cells, then isolating it. (A) expresses at least one heterologous nucleotide sequence (II) that encodes a protein (III) with methionine synthase (metF) activity. An Independent claim is also included for preparation of a feed additive (B) that contains L-methionine by culturing/fermenting an L-Met producing microorganisms, removing water from the fermentation broth and optionally also up to 100 % of the biomass, then drying the treated broth to recover (B) in powdered or granular form.

公开(公告)号：AU2003238385A1

公开(公告)日：2003-12-12

申请号：AU2003238385

申请日：2003-05-23

Applicant: BASF AG

Inventor： SCHRODER HARTWIG ,  KROGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  HAFNER STEFAN

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/10 ,  C12P13/12 ,  C12R1/15 ,  C12P11/00

Abstract: The invention relates to methods for the production of sulfur-containing fine chemicals, in particular L-methionine, by fermentation using bacteria in which a nucleotide sequence encoding an S-adenosylmethionine synthase (metK) gene is expressed.

公开(公告)号：AU2003242537A8

公开(公告)日：2003-12-02

申请号：AU2003242537

申请日：2003-05-13

Applicant: BASF AG

Inventor： KROGER BURKHARD ,  KLOPPROGGE CORINNA ,  SCHRODER HARTWIG ,  HAFNER STEFAN ,  LEE HEUNG-SHICK ,  ZELDER OSKAR

IPC: C12N15/09 ,  C07K14/34 ,  C12N15/31 ,  C12P13/08

Abstract: Isolated polypeptide sequence having the sequence of SEQ ID NO:1 or muteins thereof having the ability to bind cAMP and repress the expression of the aceB gene of C. glutamicum and which can be obtained from SEQ ID NO:1 by inserting, deleting or substituting up to 20% of the amino acids.