公开(公告)号：AU702340B2

公开(公告)日：1999-02-18

申请号：AU2476697

申请日：1997-06-06

Applicant: BIO RAD LABORATORIES

Inventor： HJERTEN STELLAN ,  LIAO JIA LI

IPC: B01J20/285 ,  C08F2/44 ,  C08F18/08 ,  C08F20/06 ,  C08F20/12 ,  C08F20/32 ,  C08F20/34 ,  C08F20/56 ,  C08F20/58 ,  C08F26/02 ,  C08F28/02 ,  G01N30/56 ,  G01N30/88 ,  B01D15/08

Abstract: Preparation of a separation medium for continuous bed reversed-phase chromatography comprises: (a) forming a mixture composed of (i) a water-soluble monomer selected from vinyl, and (meth)acrylic monomers, (ii) a crosslinking agent, (iii) an alkylmethacrylate in which the alkyl group has 3-30C, and (iv) a detergent, in an aqueous medium; and (b) polymerising (i), (ii), and (iii) in a one-step reaction. Also claimed is a preparation in which the mixture is composed of (i), (ii), (iii) a water-soluble monomer having a terminal vinyl group and a charged group, (iv) an alkyl methacrylate as described, and (v) a detergent as described, with polymerisation of (i), (ii), (iii), and (iv) taking place in a one-step reaction.

公开(公告)号：AU7577198A

公开(公告)日：1998-12-08

申请号：AU7577198

申请日：1998-05-14

Applicant: BIO RAD LABORATORIES

Inventor： HJERTEN STELLAN ,  ERICSON CHRISTER

IPC: F04B19/24 ,  F04F1/04 ,  G01N30/32

公开(公告)号：CA2162336A1

公开(公告)日：1996-07-31

申请号：CA2162336

申请日：1995-11-07

Applicant: BIO RAD LABORATORIES

Inventor： HJERTEN STELLAN ,  LIAO JIA-LI

IPC: G01N27/447 ,  C12Q1/00 ,  C12Q1/68 ,  G01N27/26 ,  G01N33/561 ,  G01N33/68

公开(公告)号：DE69020246D1

公开(公告)日：1995-07-27

申请号：DE69020246

申请日：1990-01-08

Applicant: BIO RAD LABORATORIES

Inventor： HJERTEN STELLAN

IPC: G01N30/60 ,  B01D15/08 ,  B01D15/32 ,  B01D15/34 ,  B01D15/36 ,  B01D15/38 ,  B01J20/26 ,  B01J20/281 ,  G01N30/52 ,  G01N30/88

公开(公告)号：DE60038123D1

公开(公告)日：2008-04-03

申请号：DE60038123

申请日：2000-08-29

Applicant: BIO RAD LABORATORIES

Inventor： LIAO JIA-LI ,  HJERTEN STELLAN

IPC: B01J20/285 ,  C08F2/32 ,  B01J20/26 ,  B01J39/20 ,  B01J41/14 ,  C08F18/08 ,  C08F20/04 ,  C08F20/10 ,  C08F20/54 ,  G01N30/00 ,  G01N30/88

Abstract: Chromatographic beads with pores large enough to serve as through-channels for fast diffusion and a substantial absence of pores of smaller diameters that would cause band broadening due to slow diffusive transport, are formed by suspension polymerization of an aqueous solution of a monomer mixture that includes a total monomer (combination of polymer backbone monomer and crosslinking agent) concentration of 2% to 50% by weight relative to the aqueous solution and a crosslinking agent in an amount such that the mole ratio of crosslinking agent to total monomer is from about 0.1 to about 0.7. The suspension polymerization process involves forming a suspension of aqueous droplets of the monomer mixture in an organic phase.

公开(公告)号：AU769534B2

公开(公告)日：2004-01-29

申请号：AU6945800

申请日：2000-08-29

Applicant: BIO RAD LABORATORIES

Inventor： LIAO JIA-LI ,  HJERTEN STELLAN

IPC: B01J20/285 ,  B01J20/26 ,  B01J39/20 ,  B01J41/14 ,  C08F2/32 ,  C08F18/08 ,  C08F20/04 ,  C08F20/10 ,  C08F20/54 ,  G01N30/88 ,  G01N30/48

Abstract: Chromatographic beads with pores large enough to serve as through-channels for fast diffusion and a substantial absence of pores of smaller diameters that would cause band broadening due to slow diffusive transport, are formed by suspension polymerization of an aqueous solution of a monomer mixture that includes a total monomer (combination of polymer backbone monomer and crosslinking agent) concentration of 2% to 50% by weight relative to the aqueous solution and a crosslinking agent in an amount such that the mole ratio of crosslinking agent to total monomer is from about 0.1 to about 0.7. The suspension polymerization process involves forming a suspension of aqueous droplets of the monomer mixture in an organic phase.

公开(公告)号：CA2225703C

公开(公告)日：2001-10-16

申请号：CA2225703

申请日：1997-12-22

Applicant: BIO RAD LABORATORIES

Inventor： LIAO JIA-LI ,  HJERTEN STELLAN

IPC: G01N33/50 ,  B01D15/08 ,  B01J20/281 ,  B01J20/285 ,  C08F2/10 ,  C08F18/00 ,  C08F18/08 ,  C08F20/00 ,  C08F20/06 ,  C08F20/10 ,  C08F20/56 ,  C08F20/58 ,  G01N30/56 ,  G01N30/88 ,  G01N33/68 ,  G01N30/60

Abstract: A chromatography column with a continuous solid bed spanning the cross secti on of the column, the bed containing channels large enough for hydrodynamic flow, is prepared by polymerizing a mixture of monomers and ammonium sulfate in an aqueous sol ution. The monomers include a monofunctional monomer such as a vinyl, allyl, acryli c, or methacrylic compound, and a polyfunctional monomer (i.e., a crosslinker), th e total monomer concentration being in the range of 10% to 20% by weight, the mole r atio of crosslinker to total monomer being in the range of 0.3 to 0.4, and the ammon ium sulfate having a concentration in the range of 0.4 M to 0.8 M. Functional groups to impart specialized separation capabilities, notably anion and cation exchange, can be included in the monomer mixture.

公开(公告)号：AU6945800A

公开(公告)日：2001-05-10

申请号：AU6945800

申请日：2000-08-29

Applicant: BIO RAD LABORATORIES

Inventor： LIAO JIA-LI ,  HJERTEN STELLAN

IPC: B01J20/285 ,  B01J20/26 ,  B01J39/20 ,  B01J41/14 ,  C08F2/32 ,  C08F18/08 ,  C08F20/04 ,  C08F20/10 ,  C08F20/54 ,  G01N30/88 ,  G01N30/48

Abstract: Chromatographic beads with pores large enough to serve as through-channels for fast diffusion and a substantial absence of pores of smaller diameters that would cause band broadening due to slow diffusive transport, are formed by suspension polymerization of an aqueous solution of a monomer mixture that includes a total monomer (combination of polymer backbone monomer and crosslinking agent) concentration of 2% to 50% by weight relative to the aqueous solution and a crosslinking agent in an amount such that the mole ratio of crosslinking agent to total monomer is from about 0.1 to about 0.7. The suspension polymerization process involves forming a suspension of aqueous droplets of the monomer mixture in an organic phase.

公开(公告)号：CA2225703A1

公开(公告)日：1998-07-03

申请号：CA2225703

申请日：1997-12-22

Applicant: BIO RAD LABORATORIES

Inventor： LIAO JIA-LI ,  HJERTEN STELLAN

IPC: G01N33/50 ,  B01D15/08 ,  B01J20/281 ,  B01J20/285 ,  C08F2/10 ,  C08F18/00 ,  C08F18/08 ,  C08F20/00 ,  C08F20/06 ,  C08F20/10 ,  C08F20/56 ,  C08F20/58 ,  G01N30/56 ,  G01N30/88 ,  G01N33/68 ,  G01N30/60

Abstract: A chromatography column with a continuous solid bed spanning the cross secti on of the column, the bed containing channels large enough for hydrodynamic flow, is prepared by polymerizing a mixture of monomers and ammonium sulfate in an aqueous sol ution. The monomers include a monofunctional monomer such as a vinyl, allyl, acryli c, or methacrylic compound, and a polyfunctional monomer (i.e., a crosslinker), th e total monomer concentration being in the range of 10% to 20% by weight, the mole r atio of crosslinker to total monomer being in the range of 0.3 to 0.4, and the ammon ium sulfate having a concentration in the range of 0.4 M to 0.8 M. Functional groups to impart specialized separation capabilities, notably anion and cation exchange, can be included in the monomer mixture.

公开(公告)号：FR2681944B1

公开(公告)日：1995-12-29

申请号：FR9211601

申请日：1992-09-29

Applicant: BIO RAD LABORATORIES

Inventor： HJERTEN STELLAN ,  SRICHAIYO TASANEE

IPC: G01N21/05 ,  G01N27/447 ,  G01N30/60 ,  G01N30/74 ,  G01N30/86 ,  G01N21/33

Abstract: Multiple electropherograms or chromatograms representing successive stages of an electrophoretic or chromatographic separation in a capillary are obtained by training a recorder on a succession of detection windows in the capillary, spaced apart along the capillary length. A single immovable detector is used, and the capillary is either held such that each detection window is placed in alignment with the detector in succession, or is looped such that the detection windows all lie in the path of the detector at the same time.