公开(公告)号：JPH116822A

公开(公告)日：1999-01-12

申请号：JP36031197

申请日：1997-12-26

Applicant: BIO RAD LABORATORIES

Inventor： LIAO JIA-LI ,  HJERTEN STELLAN

IPC: G01N33/50 ,  B01D15/08 ,  B01J20/281 ,  B01J20/285 ,  C08F2/10 ,  C08F18/00 ,  C08F18/08 ,  C08F20/00 ,  C08F20/06 ,  C08F20/10 ,  C08F20/56 ,  C08F20/58 ,  G01N30/56 ,  G01N30/88 ,  G01N33/68 ,  G01N30/48

Abstract: PROBLEM TO BE SOLVED: To provide a chromatography column having continuous beds formed in column from an aqueous solution without using a porogen. SOLUTION: There is provided a chromatography column having a continuous solid bet spanning the cross section of the column. The chromatography column wherein the bed contains channels large enough for hydrodynamic flow is prepared by polymerizing a mixture of monomers and ammonium sulfate in an aqueous solution. The monomer includes a monofunctional monomer such as a vinyl, allyl, acrylic, or methacrylic compound and a polyfunctional monomer (i.e., a crosslinker). The total monomer concentration is in the range of 10% to 20% by weight, the mole ratio of crosslinker to total monomer is in the range of 0.3 to 0.4 and the ammonium sulfate has a concentration in the range of 0.4 M to 0.8 M. Functional groups to impart specialized separation capabilities, notably anion and cation exchange, can be included in the monomer mixture.

公开(公告)号：JPH102891A

公开(公告)日：1998-01-06

申请号：JP6625697

申请日：1997-03-19

Applicant: BIO RAD LABORATORIES

Inventor： HJERTEN STELLAN ,  LIAO JIA-LI

IPC: B01J20/285 ,  B01D15/38 ,  B01J20/26 ,  B01J20/281 ,  B01J20/291 ,  G01N30/02 ,  G01N30/88 ,  G01N30/48

Abstract: PROBLEM TO BE SOLVED: To attain a high selective adsorption of molecules by forming a polymer from a non-functional polymer or a monomer never forming a complex with its absorbed molecule. SOLUTION: An affinity chromatographic medium in the form of a water- insoluble polymer matrix having selectivity for a preliminarily selected molecular species is formed from a non-ionic monomer chemically and physically inactive to the molecular species by polymerizing the monomer in the presence of the molecular species, and extracting the species from the resulting insoluble polymer matrix. In the use of the thus-formed polymer, every electrostatic interaction between the adsorbed molecular species in the polymer and an individual sub-unit or the formation of complex in other forms is substantially lacked. An adsorptive gel which is selective to substantially all molecular species independent from size, and has a three-dimensional molecular structure containing those having no functional group is prepared.

公开(公告)号：JPH1067811A

公开(公告)日：1998-03-10

申请号：JP15522897

申请日：1997-06-12

Applicant: BIO RAD LABORATORIES

Inventor： LIAO JIA-LI ,  HJERTEN STELLAN

IPC: B01J20/285 ,  C08F2/44 ,  C08F18/08 ,  C08F20/06 ,  C08F20/12 ,  C08F20/32 ,  C08F20/34 ,  C08F20/56 ,  C08F20/58 ,  C08F26/02 ,  C08F28/02 ,  G01N30/56 ,  G01N30/88 ,  G01N30/48

Abstract: PROBLEM TO BE SOLVED: To obtain a separating medium for reversed-phase chromatography in a single-stage reaction by polymerizing an aqueous vinyl monomer mixture containing both a specified hydrophobic alkyl methacrylate and a specified detergent. SOLUTION: The production process comprises the steps of: forming a mixture containing a water-soluble monomer (1) selected from the group consisting of vinyl monomers, acrylic monomers and methacrylic monomers, a crosslinking agent (2), an alkyl methacrylate (3) with a 3-30C alkyl and detergent (4) in an aqueous medium and polymerizing components (1) to (3) of the mixture in a single stage reaction. It is desirable that the total amount of components (1) to (3) is 2-50wt.%, and that the molar fraction of the crosslinking agent based on the total of components (1) to (3) is 0.10-0.70. The amount of the alkyl methacrylate is desirably 0.003-0.3 in terms of a molar ratio to the total of components (1) to (3).

公开(公告)号：CA2385039A1

公开(公告)日：2001-04-12

申请号：CA2385039

申请日：2000-08-29

Applicant: BIO RAD LABORATORIES

Inventor： HJERTEN STELLAN ,  LIAO JIA-LI

IPC: B01J20/285 ,  B01J20/26 ,  B01J39/20 ,  B01J41/14 ,  C08F2/32 ,  C08F18/08 ,  C08F20/04 ,  C08F20/10 ,  C08F20/54 ,  G01N30/88 ,  G01N30/48

Abstract: Chromatographic beads with pores large enough to serve as through-channels f or fast diffusion and a substantial absence of pores of smaller diameters that would cause band broadening due to slow diffusive transport, are formed by suspension polymerization of an aqueous solution of a monomer mixture that includes a total monomer (combination of polymer backbone monomer and crosslinking agent) concentration of 2 % to 50 % by weight relative to the aqueous solution and a crosslinking agent in an amount such that the mole ratio of crosslinking agent to total monomer is from about 0.1 to about 0.7. The suspension polymerization process involves forming a suspension of aqueo us droplets of the monomer mixture in an organic phase.

公开(公告)号：CA2162336C

公开(公告)日：1999-03-30

申请号：CA2162336

申请日：1995-11-07

Applicant: BIO RAD LABORATORIES

Inventor： HJERTEN STELLAN ,  LIAO JIA-LI

IPC: G01N27/447 ,  C12Q1/68 ,  G01N27/26 ,  G01N33/68

Abstract: Electrophoresis is performed in buffers which exhibit both substantial bufferingcapacity and low electrical conductivity, permitting the separations to be performed at high field strengths without loss of resolution. Four classes of buffering agents are cited as examples: (1) buffering agents with a small number of charged groups per molecule; (2) carrier ampholytes fractionated to a narrow isoelectric point range; (3) low molecular weight buffering ampholytes with an isoelectric point which is close in value to one of the pK values of the ampholyte; and (4) high molecular weight buffering ampholytes in which the acidic and basic groups have the same or very close pK values. These buffering agents are of particular interest in capillary electrophoresis.

公开(公告)号：DE69007773T2

公开(公告)日：1994-09-22

申请号：DE69007773

申请日：1990-02-13

Applicant: BIO RAD LABORATORIES

Inventor： MING DE ZHU ,  JENG-CHYH CHEN ,  HJERTEN STELLAN

IPC: B01D57/02 ,  G01N27/447

公开(公告)号：DE69024030D1

公开(公告)日：1996-01-18

申请号：DE69024030

申请日：1990-06-21

Applicant: BIO RAD LABORATORIES

Inventor： HJERTEN STELLAN

IPC: B01D57/02 ,  G01N27/447 ,  G01N27/26

Abstract: Bulk fluid movement in a capillary electrophoresis system is achieved by imposing a temperature change on either a portion of the capillary or a bulb joined to the capillary at one end. The process may be used for sample loading, in which case a small volume introduction is made, or for flushing of the capillary with buffer solution, in which a larger volume introduction is made. The temperature change may be either an increase or a decrease, and the volume passed into or through the capillary is controlled to a high degree of precision by selection and accurate measurement of the volume of the bulb or capillary portion to which the temperature change is applied together with accurate control of the temperature change itself.

公开(公告)号：GB2259980B

公开(公告)日：1995-04-05

申请号：GB9220057

申请日：1992-09-22

Applicant: BIO RAD LABORATORIES

Inventor： HJERTEN STELLAN ,  SRICHAIYO TASANEE

IPC: G01N21/05 ,  G01N27/447 ,  G01N30/60 ,  G01N30/74 ,  G01N30/86

Abstract: Multiple electropherograms or chromatograms representing successive stages of an electrophoretic or chromatographic separation in a capillary are obtained by training a recorder on a succession of detection windows in the capillary, spaced apart along the capillary length. A single immovable detector is used, and the capillary is either held such that each detection window is placed in alignment with the detector in succession, or is looped such that the detection windows all lie in the path of the detector at the same time.

公开(公告)号：GB2259980A

公开(公告)日：1993-03-31

申请号：GB9220057

申请日：1992-09-22

Applicant: BIO RAD LABORATORIES

Inventor： HJERTEN STELLAN ,  SRICHAIYO TASANEE

IPC: G01N21/05 ,  G01N27/447 ,  G01N30/60 ,  G01N30/74 ,  G01N30/86

Abstract: Multiple electropherograms or chromatograms representing successive stages of an electrophoretic or chromatographic separation in a capillary are obtained by training a recorder on a succession of detection windows in the capillary, spaced apart along the capillary length. A single immovable detector is used, and the capillary is either held such that each detection window is placed in alignment with the detector in succession, or is looped such that the detection windows all lie in the path of the detector at the same time.

公开(公告)号：DE60038123T2

公开(公告)日：2009-03-05

申请号：DE60038123

申请日：2000-08-29

Applicant: BIO RAD LABORATORIES

Inventor： LIAO JIA-LI ,  HJERTEN STELLAN

IPC: B01J20/285 ,  C08F2/32 ,  B01J20/26 ,  B01J39/20 ,  B01J41/14 ,  C08F18/08 ,  C08F20/04 ,  C08F20/10 ,  C08F20/54 ,  G01N30/00 ,  G01N30/88

Abstract: Chromatographic beads with pores large enough to serve as through-channels for fast diffusion and a substantial absence of pores of smaller diameters that would cause band broadening due to slow diffusive transport, are formed by suspension polymerization of an aqueous solution of a monomer mixture that includes a total monomer (combination of polymer backbone monomer and crosslinking agent) concentration of 2% to 50% by weight relative to the aqueous solution and a crosslinking agent in an amount such that the mole ratio of crosslinking agent to total monomer is from about 0.1 to about 0.7. The suspension polymerization process involves forming a suspension of aqueous droplets of the monomer mixture in an organic phase.