公开(公告)号：WO03027724A2

公开(公告)日：2003-04-03

申请号：PCT/US0230292

申请日：2002-09-24

Applicant: BIO RAD LABORATORIES

Inventor： NAGHIEH HARRY R ,  HILLENDAHL JAMES W ,  WALDBESER DAVID E

IPC: G01N21/25 ,  G01N21/64 ,  H04N1/04 ,  G02B

CPC classification number: G01N21/6452 ,  G01N2021/6484 ,  Y10T436/11

Abstract: Microarrays are imaged by an illumination and detection system that supplies excitation light through one or more optical fibers, each fiber transmitting excitation light from an excitation light source to a single spot in the microarray. Emission light from each spot is then collected by a collimating lens and converted to a signal that is compiled by conventional software into an image of the entire microarray. The optical fiber(s) and the collimating lens are arranged such that the direction of travel of the excitation light and the direction along which the emission light is collected are not coaxial, and preferably both are at an acute angle to the axis normal to the plane of the microarray.

Abstract translation: 微阵列通过照射和检测系统成像，该系统通过一个或多个光纤提供激发光，每个光纤将激发光从激发光源传输到微阵列中的单个点。 然后通过准直透镜收集每个斑点的发光，并将其转换为由常规软件编译成整个微阵列的图像的信号。 光纤和准直透镜被布置成使得激发光的行进方向和发射光被收集的方向不同轴，并且优选地两者都与垂直于该光束的轴成锐角 微阵列飞机。

公开(公告)号：EP2062079A4

公开(公告)日：2010-03-10

申请号：EP07813831

申请日：2007-08-07

Applicant: BIO RAD LABORATORIES

Inventor： HILLENDAHL JAMES W ,  WALDBESER DAVID E

IPC: G02B6/32 ,  G01N21/25 ,  G01N21/64 ,  G02B21/00

CPC classification number: G01N21/6452 ,  G01N2021/6421 ,  G01N2021/6471 ,  G01N2021/6484

公开(公告)号：EP2382843A4

公开(公告)日：2012-10-31

申请号：EP10736323

申请日：2010-01-27

Applicant: BIO RAD LABORATORIES

Inventor： WALDBESER DAVID E ,  BHATT NEERAJ ,  MCDONALD KEVIN A

IPC: H05B35/00 ,  B01L3/00 ,  G01N21/64 ,  G01N27/447

CPC classification number: G01N27/44721 ,  G01N27/44726 ,  G01N27/44782

公开(公告)号：EP1438615A4

公开(公告)日：2006-01-25

申请号：EP02773551

申请日：2002-09-24

Applicant: BIO RAD LABORATORIES

Inventor： NAGHIEH HARRY R ,  HILLENDAHL JAMES W ,  WALDBESER DAVID E

IPC: G01N21/25 ,  G01N21/64 ,  H04N1/04 ,  G02B6/26

CPC classification number: G01N21/6452 ,  G01N2021/6484 ,  Y10T436/11

Abstract: Microarrays (11) are imaged by an illumination and detection system that supplies excitation light through one or more optical fibers (20), each fiber transmitting excitation light from an excitation light source (14) to a single spot (12) in the microarray (11). Emission light from each spot is then collected by a collimating lens (15) and converted to a signal that is compiled by conventional software into an image of the entire microarray (11). The optical fiber(s) (20) and the collimating lens (15) are arranged such that the direction of travel of the excitation light (14) and the direction along which the emission light is collected are not coaxial, and preferably both are at an acute angle to the axis normal to the plane of the microarray.

公开(公告)号：EP1430341A4

公开(公告)日：2006-01-18

申请号：EP02763709

申请日：2002-09-24

Applicant: BIO RAD LABORATORIES

Inventor： HILLENDAHL JAMES W ,  WALDBESER DAVID E ,  NAGHIEH HARRY R

IPC: G01N21/25 ,  G01N21/64 ,  G02B6/32

CPC classification number: G01N21/253 ,  G01N21/6452 ,  G01N2021/6421 ,  G01N2021/6471 ,  G01N2021/6484

Abstract: Biochemical assays that are performed in multi-well plates (11) and that utilize a system that supplies excitation light through an optical fiber (21, 22) that transmits light from an excitation light source (14) to the well (12). Emission light produced by the excitation is then collected by a collimating lens (31) and converted to a signal that is compiled for analysis. The optical fiber (21, 22) and collimating lens (31) can either be on the same side of the receptacle or on opposite sides, i.e., one above the other below. When the optical fiber and the collimating lens are both on the open side of the receptacle, they are arranged such that the direction of travel of the excitation light and the direction along which the emission light is collected are not coaxial, and preferably both are at an acute angle to the axis normal to the mouth of the receptacle.