公开(公告)号：CA2461885C

公开(公告)日：2011-11-29

申请号：CA2461885

申请日：2002-09-24

Applicant: BIO RAD LABORATORIES

Inventor： HILLENDAHL JAMES W ,  WALDBESER DAVID E ,  NAGHIEH HARRY R

IPC: G02B6/32 ,  G01N21/25 ,  G01N21/64

Abstract: Biochemical assays that are performed in multi-well plates (11) and that utilize a system that supplies excitation light through an optical fiber (21, 22) that transmits light from an excitation light source (14) to the well (12). Emission light produced by the excitation is then collected by a collimating lens (31) and converted to a signal that is compiled for analysis. The optical fiber (21, 22) and collimating lens (31) can either be on the same side of the receptacle or on opposite sides, i.e., one above the other below. When the optical fiber and the collimating lens are both on the open side of the receptacle, they are arranged such that the direction of travel of the excitation light and the direction along which the emission light is collected are not coaxial, and preferably both are at an acute angle to the axis normal to the mouth of the receptacle.

公开(公告)号：CA2663048A1

公开(公告)日：2008-03-20

申请号：CA2663048

申请日：2007-08-07

Applicant: BIO RAD LABORATORIES

Inventor： HILLENDAHL JAMES W ,  WALDBESER DAVID E

IPC: G02B6/32 ,  G01N1/10 ,  G01N21/25 ,  G01N21/64

Abstract: Biochemical assays that utilize excitation and light emission as labels f or detection are enhanced by an illumination and detection system that suppl ies excitation light through an optical fiber that transmits excitation ligh t from an excitation light source to the sample Emission light produced by t he excitation is then collected by a lens and converted to a signal that is compiled by conventional softwar for analysis The optical fiber transfixes ( passes through) the lens via a slot or other opening and is preferably offse t from the center of the lens The optical fiber and the light collecting len s are arranged such that the direction of travel of the excitation light and the direction along which the emission light is collected are not coaxial, and preferably both are at an acute angle to the axis normal to the mouth of the receptacle.

公开(公告)号：AU2002336771C1

公开(公告)日：2005-11-17

申请号：AU2002336771

申请日：2002-09-24

Applicant: BIO RAD LABORATORIES

Inventor： WALDBESER DAVID E ,  NAGHIEH HARRY R ,  HILLENDAHL JAMES W

IPC: G01N21/25 ,  G01N21/64 ,  H04N1/04 ,  G02B006/26

Abstract: Microarrays are imaged by an illumination and detection system that supplies excitation light through one or more optical fibers, each fiber transmitting excitation light from an excitation light source to a single spot in the microarray. Emission light from each spot is then collected by a collimating lens and converted to a signal that is compiled by conventional software into an image of the entire microarray. The optical fiber(s) and the collimating lens are arranged such that the direction of travel of the excitation light and the direction along which the emission light is collected are not coaxial, and preferably both are at an acute angle to the axis normal to the plane of the microarray.

公开(公告)号：AU2002336771B2

公开(公告)日：2005-06-02

申请号：AU2002336771

申请日：2002-09-24

Applicant: BIO RAD LABORATORIES

Inventor： NAGHIEH HARRY R ,  HILLENDAHL JAMES W ,  WALDBESER DAVID E

IPC: G01N21/25 ,  G01N21/64 ,  H04N1/04 ,  G02B006/26

Abstract: Microarrays are imaged by an illumination and detection system that supplies excitation light through one or more optical fibers, each fiber transmitting excitation light from an excitation light source to a single spot in the microarray. Emission light from each spot is then collected by a collimating lens and converted to a signal that is compiled by conventional software into an image of the entire microarray. The optical fiber(s) and the collimating lens are arranged such that the direction of travel of the excitation light and the direction along which the emission light is collected are not coaxial, and preferably both are at an acute angle to the axis normal to the plane of the microarray.

公开(公告)号：CA2461499A1

公开(公告)日：2003-05-03

申请号：CA2461499

申请日：2002-09-24

Applicant: BIO RAD LABORATORIES

Inventor： NAGHIEH HARRY R ,  HILLENDAHL JAMES W ,  WALDBESER DAVID E

IPC: G01N21/25 ,  G01N21/64 ,  H04N1/04 ,  G02B6/26

Abstract: Microarrays (11) are imaged by an illumination and detection system that supplies excitation light through one or more optical fibers (20), each fibe r transmitting excitation light from an excitation light source (14) to a sing le spot (12) in the microarray (11). Emission light from each spot is then collected by a collimating lens (15) and converted to a signal that is compiled by conventional software into an image of the entire microarray (11 ). The optical fiber(s) (20) and the collimating lens (15) are arranged such th at the direction of travel of the excitation light (14) and the direction along which the emission light is collected are not coaxial, and preferably both a re at an acute angle to the axis normal to the plane of the microarray.

公开(公告)号：WO03027724A9

公开(公告)日：2004-04-29

申请号：PCT/US0230292

申请日：2002-09-24

Applicant: BIO RAD LABORATORIES

Inventor： NAGHIEH HARRY R ,  HILLENDAHL JAMES W ,  WALDBESER DAVID E

IPC: G01N21/25 ,  G01N21/64 ,  H04N1/04 ,  G02B6/26

CPC classification number: G01N21/6452 ,  G01N2021/6484 ,  Y10T436/11

Abstract: Microarrays (11) are imaged by an illumination and detection system that supplies excitation light through one or more optical fibers (20), each fiber transmitting excitation light from an excitation light source (14) to a single spot (12) in the microarray (11). Emission light from each spot is then collected by a collimating lens (15) and converted to a signal that is compiled by conventional software into an image of the entire microarray (11). The optical fiber(s) (20) and the collimating lens (15) are arranged such that the direction of travel of the excitation light (14) and the direction along which the emission light is collected are not coaxial, and preferably both are at an acute angle to the axis normal to the plane of the microarray.

Abstract translation: 微阵列（11）通过照射和检测系统成像，所述照明和检测系统通过一个或多个光纤（20）提供激发光，每个光纤将激发光源（14）的激发光传输到微阵列中的单个点（12） 11）。 然后通过准直透镜（15）收集每个斑点的发光，并将其转换为由常规软件编译成整个微阵列（11）的图像的信号。 光纤（20）和准直透镜（15）被布置成使得激发光（14）的行进方向和发射光被收集的方向不同轴，并且优选地两者都在 与垂直于微阵列平面的轴成锐角。

公开(公告)号：CA2750112A1

公开(公告)日：2010-08-05

申请号：CA2750112

申请日：2010-01-27

Applicant: BIO RAD LABORATORIES

Inventor： WALDBESER DAVID E ,  BHATT NEERAJ ,  MCDONALD KEVIN A

IPC: H05B35/00

Abstract: An adaptor is designed as an accessory to an ultraviolet transilluminator for the excitation of fluorescent molecules or labels in a planar array of biochemical samples such as a two-dimensional electrophoresis gel to enable the emissions resulting from the excitation to be detected and quantified. The adaptor is constructed to overlay the transilluminator and contains both a fluorescent dye that upon excitation by ultraviolet light emits light in the visible spectrum, and a conditioning substance that selects a portion of the wavelength band of the visible light produced by the fluorescent dye. The adaptor converts the ultraviolet light from the transilluminator to visible light while limiting the emissions reaching the detector to those that emanate from the sample. By the use of this adaptor, the transilluminator is adapted for use with samples labeled with dyes that are excitable by visible light and avoids exposure of the samples and the user to ultraviolet light.

公开(公告)号：CA2461885A1

公开(公告)日：2003-04-03

申请号：CA2461885

申请日：2002-09-24

Applicant: BIO RAD LABORATORIES

Inventor： HILLENDAHL JAMES W ,  WALDBESER DAVID E ,  NAGHIEH HARRY R

IPC: G01N21/25 ,  G01N21/64 ,  G02B6/32

Abstract: Biochemical assays that are performed in multi-well plates (11) and that utilize a system that supplies excitation light through an optical fiber (21 , 22) that transmits light from an excitation light source (14) to the well (12). Emission light produced by the excitation is then collected by a collimating lens (31) and converted to a signal that is compiled for analysi s. The optical fiber (21, 22) and collimating lens (31) can either be on the sa me side of the receptacle or on opposite sides, i.e., one above the other below . When the optical fiber and the collimating lens are both on the open side of the receptacle, they are arranged such that the direction of travel of the excitation light and the direction along which the emission light is collect ed are not coaxial, and preferably both are at an acute angle to the axis norma l to the mouth of the receptacle.

公开(公告)号：WO2008033625A3

公开(公告)日：2008-06-26

申请号：PCT/US2007075329

申请日：2007-08-07

Applicant: BIO RAD LABORATORIES ,  HILLENDAHL JAMES W ,  WALDBESER DAVID E

Inventor： HILLENDAHL JAMES W ,  WALDBESER DAVID E

IPC: G02B6/32 ,  G01N1/10 ,  G01N21/25 ,  G01N21/64

CPC classification number: G01N21/6452 ,  G01N2021/6421 ,  G01N2021/6471 ,  G01N2021/6484

Abstract: Biochemical assays that utilize excitation and light emission as labels for detection are enhanced by an illumination and detection system that supplies excitation light through an optical fiber that transmits excitation light from an excitation light source to the sample Emission light produced by the excitation is then collected by a lens and converted to a signal that is compiled by conventional softwar for analysis The optical fiber transfixes (passes through) the lens via a slot or other opening and is preferably offset from the center of the lens The optical fiber and the light collecting lens are arranged such that the direction of travel of the excitation light and the direction along which the emission light is collected are not coaxial, and preferably both are at an acute angle to the axis normal to the mouth of the receptacle.

Abstract translation: 利用激发和发光作为检测标签的生物化学测定通过照射和检测系统得到增强，所述照明和检测系统通过将激发光从激发光源传播到样品的光纤提供激发光，然后由激发产生的发射光由 透镜并转换为通过常规软件编译的信号用于分析光纤经由狭槽或其他开口经过透镜（穿过）透镜，并且优选地偏离透镜的中心。光纤和聚光透镜是 被布置为使得激发光的行进方向和发射光被收集的方向不同轴，并且优选地两者与垂直于容器的嘴的轴成锐角。

公开(公告)号：WO03027741A3

公开(公告)日：2003-07-10

申请号：PCT/US0230301

申请日：2002-09-24

Applicant: BIO RAD LABORATORIES

Inventor： HILLENDAHL JAMES W ,  WALDBESER DAVID E ,  NAGHIEH HARRY R

IPC: G01N21/25 ,  G01N21/64 ,  G02B6/32

CPC classification number: G01N21/253 ,  G01N21/6452 ,  G01N2021/6421 ,  G01N2021/6471 ,  G01N2021/6484

Abstract: Biochemical assays that are performed in multi-well plates 11 and that utilize a system that supplies excitation light through an optical fiber 21, 22 that transmits light from an excitation light source 14 to the well 12. Emission light produced by the excitation is then collected by a collimating lens 31 and converted to a signal that is compiled for analysis. The optical fiber 21, 22 and collimating lens 31 can either be on the same side of the receptacle or on opposite sides, i.e., one above the other below. When the optical fiber and the collimating lens are both on the open side of the receptacle, they are arranged such that the direction of travel of the excitation light and the direction along which the emission light is collected are not coaxial, and preferably both are at an acute angle to the axis normal to the mouth of the receptacle.

Abstract translation: 在多孔板11中进行并且利用通过光纤21,22提供激发光的系统的生物化学测定，所述光纤21,22将来自激发光源14的光传输到井12.然后收集由激发产生的发射光 通过准直透镜31并被转换为编译用于分析的信号。 光纤21,22和准直透镜31可以在插座的同一侧上或在相对侧上，即在下面一个在另一个之上。 当光纤和准直透镜均在插座的开口侧时，它们被布置为使得激发光的行进方向和收集发射光的方向不同轴，并且优选地两者都处于 与插座的嘴部垂直的轴线成锐角。