公开(公告)号：SG10201402770YA

公开(公告)日：2014-08-28

申请号：SG10201402770Y

申请日：2010-04-02

Applicant: FLUIDIGM CORP

Inventor： MAY ANDREW ,  CHEN PEILIN ,  WANG JUN ,  KAPER FIONA ,  ANDERSON MEGAN

Abstract: The present invention provides an elastomer microfluidic device that enables simultaneous amplification of multiple nucleic acid samples with multiple pairs of primers in a matrix format as well as recovery of the products e.g. for next generation sequencing. The flow of samples and reagents as well as the compartmentalization of individual reactions in the multiple channels and chambers is controlled using valves and dilation pumping / volumetric capacitive pumping.

公开(公告)号：CA3018687A1

公开(公告)日：2010-10-07

申请号：CA3018687

申请日：2010-04-02

Applicant: FLUIDIGM CORP

Inventor： ANDERSON MEGAN ,  CHEN PEILIN ,  KAPER FIONA ,  MAY ANDREW ,  WANG JUN

IPC: G01N33/48 ,  B81B3/00 ,  B81B7/04 ,  C12M1/34 ,  C12M1/36 ,  C12Q1/6853

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

公开(公告)号：AU2015242980A1

公开(公告)日：2015-11-05

申请号：AU2015242980

申请日：2015-10-13

Applicant: FLUIDIGM CORP

Inventor： MAY ANDREW ,  CHEN PEILIN ,  WANG JUN ,  KAPER FIONA ,  ANDERSON MEGAN

IPC: C12Q1/68

Abstract: [0277] In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

公开(公告)号：DK2414547T3

公开(公告)日：2014-06-16

申请号：DK10759511

申请日：2010-04-02

Applicant: FLUIDIGM CORP

Inventor： MAY ANDREW ,  CHEN PEILIN ,  WANG JUN ,  KAPER FIONA

IPC: C12Q1/68

公开(公告)号：CA2757560A1

公开(公告)日：2010-10-07

申请号：CA2757560

申请日：2010-04-02

Applicant: FLUIDIGM CORP

Inventor： MAY ANDREW ,  CHEN PEILIN ,  WANG JUN ,  KAPER FIONA ,  ANDERSON MEGAN

IPC: C12Q1/68 ,  B81B7/04 ,  C12M1/36 ,  C12P19/34 ,  C40B20/04 ,  C40B30/04 ,  C40B70/00 ,  G01N33/50

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

公开(公告)号：CA2874343C

公开(公告)日：2021-11-09

申请号：CA2874343

申请日：2013-05-21

Applicant: FLUIDIGM CORP

Inventor： ANDERSON MEGAN ,  CHEN PEILIN ,  FOWLER BRIAN ,  KAPER FIONA ,  LEBOFSKY RONALD ,  MAY ANDREW

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/6869 ,  C40B30/00 ,  C40B70/00 ,  G01N1/28 ,  G01N1/38 ,  G01N33/48

Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analytic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out "in parallel," i.e., essentially simultaneously in the separate reaction volumes.

公开(公告)号：CA2757560C

公开(公告)日：2018-11-13

申请号：CA2757560

申请日：2010-04-02

Applicant: FLUIDIGM CORP

Inventor： MAY ANDREW ,  CHEN PEILIN ,  WANG JUN ,  KAPER FIONA ,  ANDERSON MEGAN

IPC: C12Q1/68 ,  B81B7/04 ,  C12M1/34 ,  C12M1/36 ,  C12P19/34 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/686 ,  C40B20/04 ,  C40B30/04 ,  C40B70/00 ,  G01N33/50

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

公开(公告)号：EA023190B1

公开(公告)日：2016-05-31

申请号：EA201171206

申请日：2010-04-02

Applicant: FLUIDIGM CORP

Inventor： MAY ANDREW ,  CHEN PEILIN ,  WANG JUN ,  KAPER FIONA ,  ANDERSON MEGAN

IPC: C12Q1/68

Abstract: Вопределенныхвариантахосуществленияданноеизобретениеобеспечиваетспособыамплификации, вкоторыхнуклеотиднуюметку(и) и, факультативно, штрих-кодовуюнуклеотиднуюпоследовательностьдобавляютк целевымнуклеотиднымпоследовательностям. Вдругихвариантахосуществленияданноеизобретениеобеспечиваетмикрофлюидноеустройство, котороевключаетмножествопервыхлинийвводаи множествовторыхлинийввода. Микрофлюидноеустройствотакжевключаетмножествонаборовпервыхкамери множествонабороввторыхкамер. Каждыйнаборпервыхкамернаходитсяв жидкостнойсвязис однимизмножествапервыхлинийввода. Каждыйнаборвторыхкамернаходитсяв жидкостнойсвязис однимизмножествавторыхлинийввода. Микрофлюидноеустройстводополнительновключаетмножествопервыхплунжерныхпарв жидкостнойсвязис первойчастьюмножествавторыхлинийвводаи множествовторыхплунжерныхпарв жидкостнойсвязис второйчастьюмножествавторыхлинийввода.

公开(公告)号：AU2010232439B2

公开(公告)日：2015-07-16

申请号：AU2010232439

申请日：2010-04-02

Applicant: FLUIDIGM CORP

Inventor： MAY ANDREW ,  CHEN PEILIN ,  WANG JUN ,  KAPER FIONA ,  ANDERSON MEGAN

IPC: C12Q1/68

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

公开(公告)号：EA023190B9

公开(公告)日：2018-03-30

申请号：EA201171206

申请日：2010-04-02

Applicant: FLUIDIGM CORP

Inventor： MAY ANDREW ,  CHEN PEILIN ,  WANG JUN ,  KAPER FIONA ,  ANDERSON MEGAN

IPC: C12Q1/68

Abstract: Вопределенныхвариантахосуществленияданноеизобретениеобеспечиваетспособыамплификации, вкоторыхнуклеотиднуюметку(и) и, факультативно, штрих-кодовуюнуклеотиднуюпоследовательностьдобавляютк целевымнуклеотиднымпоследовательностям. Вдругихвариантахосуществленияданноеизобретениеобеспечиваетмикрофлюидноеустройство, котороевключаетмножествопервыхлинийвводаи множествовторыхлинийввода. Микрофлюидноеустройствотакжевключаетмножествонаборовпервыхкамери множествонабороввторыхкамер. Каждыйнаборпервыхкамернаходитсяв жидкостнойсвязис однимизмножествапервыхлинийввода. Каждыйнаборвторыхкамернаходитсяв жидкостнойсвязис однимизмножествавторыхлинийввода. Микрофлюидноеустройстводополнительновключаетмножествопервыхплунжерныхпарв жидкостнойсвязис первойчастьюмножествавторыхлинийвводаи множествовторыхплунжерныхпарв жидкостнойсвязис второйчастьюмножествавторыхлинийввода.